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The Future of Gene Therapy: Revolutionizing Modern Medicine Akram, Muhammad; Khan, Alam Afroz Urooj; Umaru, Isaac John
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i2.5326

The use of gene therapy paired with advanced technology may one day enable chronic illnesses, cancerous diseases, and even genetic defects to be treated via DNA alterations. With developments and promising results in gene CRISPR-Cas9 editing technology, improved treatments and promising results from clinical trials have made gene therapy a possibility. It is also predicted that gene therapy will be used in the future to combat heart issues, cancer, neurological diseases, and other hereditary attributes. Nonetheless, metastatic issues pertaining to ethics, engagement, spending, and the probability of negative consequences over time still remain. In the case of augmented medicine, incorporating stem cells with new artificial intelligence technology will ensure better and more accurate treatment along with greater personalization of gene therapies. This is expected to lead to a whole new realm of precision medicine.

Effects of Shea Butter. (Vitellaria paradoxa) Bark Extracts as Root Dips on Root-knot Nematodes (Meloidogyne Spp) Infestation and Growth of Tomato (Lycepersicon Iycopersicum L.) in Adamawa, Nigeria Jada, M. Y.; Zirafilla, O. A.; M., Maryam Y.; J, Akwayamai P.; O, Pheobe A.; Timon, Freedom
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i2.5385

The management of root-knot nematodes(Meloidogyne spp.) presents significant challenges, particularly when synthetic (artificial) chemicals are employed. These chemicals are expensive for Nigerian farmers who lack resources and pose health and environmental risks. As a result, an experiment was conducted to evaluate the effectiveness of plant extracts, which are readily soluble and may be less expensive, as a root dip. A field study was carried out using root dips for tomato seedlings to evaluate the effectiveness of shea butter bark extracts against Meloidogyne spp. infestation. The field experiments were set up in a split plot design at the Loko and Mayo Belwa locations. There were sixteen treatments that were replicated three times, with four different concentrations (control, 10, 30, and 60%) and four different exposure times (5, 15, 30, and 60 minutes). The main plot was given concentrations, and the subplots were given exposure times. Data on the number of branches, fresh and dry shoot weight (g), and plant height (cm) following transplanting (WAT) were collected from the two sites. Galling index and fresh and dry root weight (g). ANOVA was used to analyze the data, and at the five percent probability level, means were separated using the Least Significant Difference (LSD). The field experiment's establishment and growth results at the Loko and Mayo Belwa locations demonstrated that the root dip exposed to 60% concentration in shea butter bark extracts for 60 minutes produced significant results (P=0). At 4, the plant height is higher (05). At Loko, WAT is 45.92, 69.75, and 73.25 cm, while at Mayo Belwa, it is 40.00, 57.00, and 67.25 cm. Additionally, it demonstrated a highly significant (P=0.01) difference between concentration and duration at 4.8, a significant (P=0.05) interaction between location and concentration at all times (4,8, and 10 WAT), and a significant (P=0.05) interaction between location and duration at 4WAT. 10WAT in addition to a significant (P=0.05) location difference. duration and focus at 4WAT exclusively. One could draw the conclusion that V. Extracts from paradoxa promoted establishment and growth by preventing or discouraging Meloidogyne spp. from attacking the dipped roots.

Biosafty Evaluation of “Makann” A Bi-herbal Formulation on Female Mice: Kidney and Liver Itemire, Anne Oghenekevwe; Idu, MacDonald; Enitome, Bafor Evi; Eze, Gerald Ikechi; Gabriel, Benjamin Ogunma
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i2.5424

This study evaluated the biosafety of “Makann”, a bi-herbal formulation (Garcinia kola and Carica papaya), on the kidneys and liver of female mice. The collected, washed, shade-dried and processed plant extract (the biherbal formulation) was administered in various groups of female mice at 2 g/kg as a single dose for 24 hours and 14 days, and 14 days daily dose, focusing on body weight, temperature, haematological parameters, organ weight, and histology of the organs using standard analytical methods. The results indicated the biherbal formulated extract at 2 g/kg had no significant difference in the body weight and temperature of the treated animals across single doses of 24 hr and 14 days and 14 daily doses when compared with the control. There was a significant increase in white blood cell count, lymphocyte count, red blood cell count, hemoglobin level, and mean corpuscle hemoglobin concentration in the 24-hr single-dose treated group. Platelet count was significantly increased in all treatment groups compared with the control (p<0.05). The effects of the biherbal extract showed no significant difference in organ weights (liver and kidney) in single doses of 24 hr and 14 days, and daily doses of 14 days when compared with the control groups (p<0.05). The histopathological evaluation of the understudied vital organs revealed no lesions or signs of hepatorenal toxicity across the treatment groups when compared with the control. The insignificant toxicity observed in the biherbal formulation across the studied parameters suggested that the biherbal formulation may exhibit less or no toxicity at the studied dose of 2000 mg/kg. In conclusion, the biherbal formulation may be used at 2 g/kg for safety purposes; further studies on its safety at long-term and other dose administrations should be carried out.

Emerging Applications and Challenges of Nanotechnology in Medicine and Nutrition Akram, Muhammad; Mahmood, Abid; Hasan, Mohammed Khudhair; Umaru, Isaac John; Abdulghafoor, Hind A.; Khan, Fahad Said; Ozdemir, Fethi Ahmet; Sołowski, Gaweł; Ali, Jaouher Ben
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i2.5484

Emerging applications of nanotechnology in medicine and nutrition present both significant opportunities and challenges that warrant thorough investigation. This study aims to explore the intersection of nanotechnology and genomic nutrition, focusing on how these advancements can enhance preventive care and health outcomes. Employing a qualitative descriptive methodology, we conducted a comparative analysis of existing literature and case studies to assess the implications of these technologies on dietary customization and patient-provider relationships. Our findings reveal that nanotechnology facilitates the development of targeted nutritional interventions that align with individual genetic profiles, thereby improving adherence to dietary guidelines and mitigating disease risk. Furthermore, we identify that the integration of continuous health monitoring technologies fosters a collaborative partnership between patients and healthcare providers, enhancing communication and decision-making processes in treatment and preventive initiatives. The implications of this research underscore the necessity for healthcare systems to adopt technological advancements to address the complexities of modern medicine effectively. By embracing these innovations, the potential for precision medicine is significantly amplified, paving the way for improved health outcomes and personalized nutrition strategies in the future.

Musashi Proteins as Prognostic Biomarkers: Role in Leukemic Cancer and Stem Cells Growth Baig, Muhammad Haris; Umaru, Isaac John
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i2.5581

Leukemia stem cell's (LSC) ability to self-renew and survive depends on the RNA-binding regulators known as Musashi proteins (MSI1 and MSI2). By stabilizing the mRNAs of key oncogenes like HOXA9 and MYC, MSI2 encourages leukemia growth and treatment resistance, especially in acute myeloid leukemia (AML). On the other hand, MSI1 enhances Notch1 signaling, which helps explain the traits of cancer stem cells in leukemia and solid tumors. Since dysregulation of these proteins is linked to recurrence, treatment resistance, and poor prognosis, they are crucial therapeutic targets. Preclinical research indicates that treatments targeting MSI proteins have potential results. Small-molecule inhibitors and RNA-based methods are being developed to disrupt MSI RNA connections, lowering LSC self-renewal and enhancing chemotherapeutic responses. Inhibiting MSI2 can reduce key pathways such as β-catenin and STAT3, improving therapeutic success in AML. CRISPR-Cas9 technology has also shown promise in overcoming therapeutic resistance by deactivating MSI2.

Evolution of DNA Technology in Treating Animal Diseases Akram, Muhammad; Umaru, Isaac John; Mahmood, Abid; Hasan, Mohammed Khudhair; Abdulghafoor, Hind A.; Khan, Fahad Said; Ozdemir, Fethi Ahmet; Sołowski, Gaweł; Ali, Jaouher Ben; Al-Musawi, Jehan Mohammad
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i2.5582

A single gene mutation can result in aberrant cell activity and the production of a faulty protein. The cell will certainly operate very poorly or not at all as a result of this mutation. Given that the genetic material may be a therapeutic agent; this might be seen as qualitatively distinct from other conventional drugs. Gene therapy may be able to rectify or perhaps cure the pathophysiology of a disease by changing the genetic composition of cells. In veterinary medicine, genetic engineering has been used to xenografts, and detects, prevent, and treat illnesses. Among the challenges that gene therapy faces include transfection, intracellular vector stability, cellular and nuclear entrance, and ethical concerns. Deficits in gene transfer vectors and a lack of knowledge about the biological interactions between these vectors and the host are two examples of fundamental challenges.

Microbial Genetics: Foundations, Applications, and Future Directions in Science and Biotechnology Akram, Muhammad; Umaru, Isaac John; Mahmood, Abid; Hasan, Mohammed Khudhair; Abdulghafoor, Hind A.; Khan, Fahad Said; Ozdemir, Fethi Ahmet; Sołowski, Gaweł; Ali, Jaouher Ben; Al-Musawi, Jehan Mohammad
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i2.5652

This field is essential to comprehending not just basic biological processes but also how they are used in biotechnology, agriculture, and medicine. Microbial genetics is the study of genetic material, which includes horizontal gene transfer, DNA replication, gene expression, and mutation. Many microorganisms, in contrast to larger species, have genomes that are small and relatively basic, enabling researchers to accurately examine the regulation and function of genes. Mechanisms like transformation, transduction, and conjugation, which speed up the acquisition and spread of genetic characteristics like antibiotic resistance, are primarily responsible for the genetic plasticity of microorganisms, especially bacteria. Given that existing treatment approaches are being challenged by the growth consequences. Additionally, using cutting-edge molecular methods like whole-genome sequencing, recombinant DNA technology, and CRISPR-Cas systems, microbial genetics aids in the creation of novel antibiotics, vaccines, and diagnostic instruments. The intricacies of microbial communities, or microbiomes, and their interactions with hosts and the environment have also been clarified by research in microbial genetics. Our knowledge of microbial ecology, pathogenicity, and symbiosis has increased as a result. The discipline is still developing in industrial settings, but the combination of systems genetics and computational biology holds promise for deciphering intricate regulatory networks and enabling predictive modeling of microbial behavior. As microbial genetics advances, ethical issues—especially those pertaining to gene editing and the discharge of genetically engineered organisms—become more significant. To sum up, microbial genetics is a fundamental component of contemporary biology and biotechnology, offering deep understanding of microbial life and laying the groundwork for advancement in a wide range of scientific and industrial domains. Addressing global health, sustainability, and environmental management concerns requires ongoing study in this area.

Cell Dynamics: Mechanisms, Technologies, and Applications in Health and Disease Akram, Muhammad; Umaru, Isaac John; Mahmood, Abid; Hasan, Mohammed Khudhair; Abdulghafoor, Hind A.; Khan, Fahad Said; Ozdemir, Fethi Ahmet; Sołowski, Gaweł; Ali, Jaouher Ben; Al-Musawi, Jehan Mohammad
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i2.5653

The intricate and well-coordinated activities of cells in response to both internal and external stimuli are referred to as cell dynamics. These behaviors help living things grow, differentiate, maintain, and adapt. Using a variety of interdisciplinary techniques from molecular biology, biophysics, computational modeling, and live-cell imaging, this field examines the dynamic processes that control cell morphology, intracellular transport, cytoskeletal reorganization, signal transduction, and cell motility. The capacity of cells to alter their structure and function in real time, allowing for quick reactions to changes in the environment, damage, or developmental signals, is a key component of cell dynamics. The complex coordination of cytoskeletal filaments, vesicle trafficking channels, and signaling networks that underpin activities including mitosis, migration, immunological surveillance, and synaptic plasticity has been made visible by developments in high-resolution imaging and single-cell analysis. Recent research has demonstrated that cellular activities incorporate stochastic fluctuations, spatial compartmentalization, and emergent features resulting from the combined actions of molecular complexes, in addition to deterministic biochemical routes. It has been demonstrated that the mechanical characteristics of the cellular microenvironment, such as the extracellular matrix composition and substrate stiffness, are essential for regulating dynamic cellular responses, especially during wound healing, tissue formation, and cancer progression. Additionally, the enormous datasets produced by live-cell imaging and omics technologies are being decoded more and more using computational models and artificial intelligence tools, which offer fresh perspectives on the temporal and spatial control of cellular activities. In addition to expanding our understanding of basic cell biology, an understanding of cellular dynamics may help develop therapeutic approaches that target dysregulated cellular activities in conditions including cancer, dementia, and immunological disorders. In order to anticipate cellular outcomes and manipulate cell destiny for applications in synthetic biology and regenerative medicine, it will be crucial to combine quantitative modeling with experimental data as we continue to clarify the fundamentals of cellular structure and adaptability.

Comparative Study on the Proximate and Amino Acids Levels in Selected Edible Mushroom Species Adebisi, Arowora Kayode; Joseph, Iornenge Terungwa; Adams, Idris Habiba; Mohammed, Ikrimah Usman; David, Bando Christopher; Efoseh, Danlami; Umaru, Isaac John
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i2.5892

Mushrooms represent an ancient group of heterotrophic organisms classified under Thallophytae, and based on their chemical composition and utility, they are broadly categorized as edible or poisonous. Edible mushrooms can further be divided into wild and cultivated types. This study compares the amino acid profiles and proximate compositions of two edible mushrooms, tea tree mushroom (Agrocybe aegerita) and oyster mushroom (Pleurotus ostreatus), collected from Takum Local Government Area of Taraba State, Nigeria. Freshly harvested samples were air-dried for three days, pulverized, and subjected to amino acid analysis using isocratic high-performance liquid chromatography (HPLC) and proximate analysis following standard protocols from the Association of Official Analytical Chemists (AOAC). Results showed that Agrocybe aegerita exhibited higher concentrations of amino acids compared to Pleurotus ostreatus, with a non-significant decrease (p > 0.05) observed in Pleurotus ostreatus for essential and non-essential amino acids including lysine, methionine, threonine, isoleucine, leucine, phenylalanine, valine, tryptophan, histidine, arginine, serine, cysteine, tyrosine, alanine, aspartic acid, glutamic acid, glycine, and proline. Conversely, Pleurotus ostreatus demonstrated slightly higher values in proximate components such as carbohydrate, fiber, ash, and moisture, whereas Agrocybe aegerita showed higher but non-significant values in protein, fat, and energy content. The study concludes that Agrocybe aegerita is nutritionally superior in terms of amino acid composition, while Pleurotus ostreatus is marginally better in proximate nutritional content, supporting their complementary roles in dietary applications.

Zinc Oxide Based Nanomaterials and Their Application in the Removal of Toxic Contaminants for Waste Water Matilda, Manasseh Ilumunter; Umaru, Isaac John; Umaru, Kerenhappuch Isaac; Akram, Muhammad
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i2.5893

The increasing global demand for clean water underscores the urgency of addressing environmental pollution, particularly through effective wastewater treatment. Rapid urbanization, population growth, and industrial expansion have significantly contributed to the degradation of water quality, making contamination a persistent and complex challenge. Despite numerous scientific efforts to develop remediation strategies, many existing techniques remain difficult to replicate or scale down effectively for widespread application. In this context, nanotechnology has emerged as a promising field, offering innovative solutions for environmental remediation. Among various nanomaterials, zinc oxide (ZnO) nanoparticles stand out due to their unique physicochemical and optical properties, which are substantially enhanced at the nanoscale. ZnO nanoparticles have gained considerable attention in material science, especially for their applications in photocatalysis, antimicrobial treatments, and environmental detoxification. However, their synthesis and functional performance are highly sensitive to several factors, including the pH of the reaction medium, calcination temperature, reaction time, stirring speed, the nature of capping agents, and the concentration of metal precursors. These variables critically influence the size, morphology, and surface activity of ZnO nanoparticles, thereby determining their effectiveness in water treatment applications. Understanding and optimizing these synthesis parameters are crucial for enhancing the reproducibility, efficiency, and scalability of ZnO-based nanotechnologies in real-world environmental contexts.

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M. Irwan Hadi

Contact Email
office@yasin-alsys.org

Phone
+6285799379817

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Editorial Address
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Location
Kab. lombok timur,

Nusa tenggara barat

INDONESIA

Abstract

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Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name M. Irwan Hadi

Contact Email office@yasin-alsys.org

Phone +6285799379817

Journal Mail Official -

Editorial Address -

Location Kab. lombok timur, Nusa tenggara barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
M. Irwan Hadi

Contact Email
office@yasin-alsys.org

Phone
+6285799379817

Journal Mail Official
-

Editorial Address
-

Location
Kab. lombok timur,

Nusa tenggara barat

INDONESIA