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Jurnal Penelitian Saintek

saintek
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Published by Universitas Negeri Yogyakarta

ISSN : 14123991 EISSN : 25287036 DOI : 10.21831

Core Subject : Science,

Decision Sciences, Operations Research & Management

Arjuna Subject : -

Articles 286 Documents

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EFFECT OF MORDAN SYNTHESIS OF CANNED WASTE TO POWER IKAT DAN LAJU REMOVED METHYL VIOLET COLOR DYES BY FABRIC FIBER EFFECT OF MORDAN SYNTHESIS OF CANNED WASTE TO POWER Jaslin Ikhsan; Endang Widjayanti LFX; Sunarto Sunarto
Jurnal Penelitian Saintek Vol 19, No 1: April 2014
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

This study aims to determine: the binding capacity of cloth toward methylene violet (MV) dye, the rate of releaseof methyl violet from cloth due to washing, and the effect of the addition of the mordant to the binding capacity and the rate of release. pH, temperature, and equilibrium time were always maintained in all experiments. Data of binding capacity were collected by isothem experiments and performed by measuring the concentration of methyl violet bound by cloth at various added-concentrations of methylene violet. Collected data were analyzed with a Langmuir and Freundlich isotherm equations. Data of release rate were collected by mesuring the concentration of free methylene violet dye in solution due to washing as a function of time. The release data were analyzed using the Lagergren rate equation. The analysis showed that bonding between methylene violet and cloth occurs with releasing protons and via hydrogen bonds. Binding capacity was represented by the Freundlich isotherm equation on which the capacity was decreased by the addition of mordant, with represented bythe constants of k 1 , 2.45 x 10 -8 and 1.75 x 10-8 M MV per gram of cloth, respectively. The addition of mordant also deceleratedoubly the releaseof MV from cloth due to washing whose constants k - 2 were 2103.039 and 1013.958 mmol g-1 min-1, respectively.

THE EFFECT OF PARTICLE SIZE OF VOLCANIC ASH AND PHOSPHATE ROCK IN ANDISOL Nur Azizah; Mahfud Arifin; Maya Damayani
Jurnal Penelitian Saintek Vol 24, No 1: April 2019
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

This study was aimed at determining the effect of various particle sizes of volcanic ash and phosphate rock, including normal size (2mm), micron (200 µm) and nano (400 ղ m) on available P, P retention, delta pH and leaf area of tea plant seeds in Ciater PTPN XII Tea Plantation, West Java. The study was carried out in December 2017 until February 2018. The soil analysis was carried out at the Integrated Testing Laboratory, Vegetable Crops Research Institute, Lembang, West Java. The manufacture of micron and nano volcanic ash and phosphate rock particles was carried out at the Nanotechnology and Graphine Laboratory, Mathematics and Science Faculty, Padjadjaran University and at Tekmira Research Center, Bandung. The research design used was a Randomized Block Design (RBD) consisting of 10 treatments of the combination of volcanic ash and phosphate rocks which were repeated three times. The results show that there is a significant effect of volcanic ash and phosphate rocks of normal size to a decrease in P retention of up to 42.87%. The combination of the treatment of normal-sized volcanic ash and nano-sized phosphate rocks has a significant effect on the total leaf area of the Gambung 7 clone plant, but it did not significantly affect the available P and delta pH.PENGARUH UKURAN PARTIKEL ABU VULKANIK DAN BATUAN FOSFAT PADA ANDISOLPenelitian ini bertujuan untuk mengetahui pengaruh pemberian berbagai ukuran partikel abu vulkanik dan batuan fosfat, meliputi ukuran normal (2mm), mikron (200 µm) dan nano (400 ղm) terhadap P tersedia, retensi P, delta pH dan luas daun bibit tanaman teh di Perkebunan Teh PTPN XII Ciater, Jawa Barat. Penelitian lapangan dilaksanakan pada Desember 2017 sampai Februari 2018. Analisis tanah dilakukan di Laboratorium Penguji Terpadu, Balai Penelitian Tanaman Sayuran, Lembang, Jawa Barat. Pembuatan partikel mikron dan nano abu vulkanik dan batuan fosfat dilakukan di Laboratorium Nanoteknologi dan Graphine, Fakultas MIPA, Universitas Padjadjaran dan di Puslitbang Tekmira, Bandung. Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok (RAK) yang terdiri atas 10 perlakuan kombinasi ukuran abu vulkanik dan batuan fosfat yang diulang sebanyak tiga kali. Hasil penelitian ini menunjukkan bahwa terdapat pengaruh nyata pemberian abu vulkanik dan batuan fosfat yang berukuran normal terhadap penurunan retensi P hingga 42,87%. Kombinasi perlakuan abu vulkanik berukuran normal dan batuan fosfat berukuran nano berpengaruh nyata terhadap total luas daun tanaman teh klon Gambung 7, tetapi tidak berpengaruh nyata terhadap P tersedia dan delta pH.

EFFECT OF DIFFERENT METHODS OF COMPONENTS SOURCE OF OUTLINE OF NEW PLANT OIL (Artemisia vulgaris L) AND AN EFFECT OF ANTIBOYTE Hartati Sulastri; Elizabeth Betty Elok; Lilik Linawati
Jurnal Penelitian Saintek Vol 19, No 2: Oktober 2014
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Mugwort (Artemisia vulgaris L) from Tawangmangu Central Java was extracted by water distillation, water steam and steam distillation. Essential oil Mugwort were analized by GCMS. The result showed that the chemical composition of mugwort essential oil from water distillation were composed of 36 compounds, water-steam distillation showed 34 compounds whereas steam distillation showed 29 compounds. Nevertheless three distillation methods gave similar 6 dominant compounds in different concentration. Six dominant compounds were 3,5-dimethyl-4-ethylidene-cyclohex-2-ene-1-one, filifolone, germacrene-D, gamma-caryophyllene, eucarvone and 1,8-cineol, the amounts of each of them was more than 3%. Bioautography test showed that mugwort essential oil have a good antibacterial effect especially to E.coli bacteria

PRODUKSI PIGMEN DAN ASAM γ-AMINOBUTIRAT (GABA) OLEH Monascus purpureus PADA KONSENTRASI INOKULUM DAN WAKTU INKUBASI YANG BERBEDA Maria Sarah Fadillah; Endang Kusdiyantini; Wijanarka Wijanarka
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Penelitian ini bertujuan untuk mengetahui produksi pigmen dan GABA oleh M. purpureus dengan konsentrasi inokulum dan waktu inkubasi yang berbeda. Fermentasi dilakukan menggunakan metode fermentasi cair dengan konsentrasi inokulum serta waktu inkubasi yang berbeda. Pengukuran pigmen intraselular dilakukan dengan mengekstraksi pelet sel menggunakan etanol 95%. Produksi GABA ditentukan menggunakan metode ninhidrin. Spektrofotometer digunakan untuk mengukur pigmen pada panjang gelombang 500, 470, dan 400 nm, sementara GABA pada panjang gelombang 401 nm. Penelitian menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan dua faktor. Hasil penelitian menunjukkan rerata nilai pigmen merah, jingga dan kuning tertinggi pada pigmen ekstraseluler (P0,05) terjadi pada perlakuan C3, sementara pigmen intraseluler (P0,05) pada perlakuan C1, dengan perlakuan waktu inkubasi (P0,05) W14. Interaksi antar kedua perlakuan (C*W) terjadi pada pigmen ekstraseluler merah dan jingga (P0,05). Produksi GABA tidak berbeda secara signifikan pada pada perlakuan konsentrasi inokulum (P0,05), tetapi berbeda secara signifikan pada perlakuan waktu inkubasi (P0,05) dan perlakuan W14 menunjukkan produksi tertinggi (6,1085 mg/ml). Tidak adanya interaksi antardua perlakuan dalam produksi GABA (P0,05).PRODUCTION OF PIGMENTS AND γ-AMINOBUTYRIC ACID (GABA) BY Monascus purpureusThis study was aimed at examining the production of pigments and GABA by M. purpureus in varied inoculum concentration and incubation time. The fermentation was carried out by submerged fermentation method with inoculum concentration and varied incubation time. Cell pellet was extracted using 95% ethanol for intracellular pigment measurement. GABA production was determined by ninhydrin method. Pigments were measured at 500, 470, and 400 nm wavelength by spectrophotometry, and GABA was measured at 401 nm wavelength. The experimental design was Completely Randomize Design (CRD) factorial with two factors. The higher colour value of extracellular (P0,05) red, orange and yellow pigments showed at C3 while intracellular (P0,05) showed at C1 with incubation time at W14. There are some interactions between two factors (C*W) for red and orange extracellular pigments (P0,05). It was observed that inoculum concentrations have no significant difference (P0,05) for GABA production. In other hand, there is significant difference for incubation time factors (P0,05) with the highest production at W14 (6,1085 mg/ml). There is no interaction between two factors for GABA production (P0,05).

EFFECT OF SODIUM BISULFIT CONCENTRATION TO VITAMIN C RESIDENCE, TOTAL RESIDUAL SULFITE AND CONSUMER EQUIPMENT LEVEL ON HAIR SIRUP Nani Ratnaningsih; Yuriani Yuriani
Jurnal Penelitian Saintek Vol 10, No 2: Oktober 2005
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Effect of concentration of sodium bisulfite to vitamin C content, totalsulfite residue and consumer hedonic level of rambutan syrup has been studied. Research method was experiment by complete random design. Vitamin C content was analyzed by the iodium titration, the total sulfite residue by distillation analysis (Rangana, 1977), and consumer hedonic level by hedonic scale test with 80 untrained panelists. Analysis data was done by analysis variance at 5% level of significance and continued with the Tukey test. Based on research result showed that concentration of sodium bisulfite have a significant effect to vitamin C content and total sulfite residue of rambutan syrup. Vitamin C content and total sulfite residue increased with the increasing concentration of sodium bisulfite. Concentration of sodium bisulfite has a significant effect to aroma of rambutan syrup, otherwise no influence at color andflavor.

EFFECT OF ALGINAT CONCENTRATION VARIATION AND LIPASE CONCENTRATION TO HYDROLYSIS ACTIVITY AND SPECIFIC ACTIVITY OF AMPILE LIPASE IN LIPASE AMOBILIZATION PROCESS FROM Rhizopus delemar Nani Ratnaningsih
Jurnal Penelitian Saintek Vol 9, No 1: April 2004
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Effect of lipasefrom Rhizopus delemar and alginate concentration during immobilization preparation on the hydrolytic activity and the specific activity of immobilized lipase has been studied Immobilization of lipase was done by entrapping lipase onto Ca-ulginate beads with 0.2 M CaC/] Immobilized lipase made from 9 variations bv mixing lipase at same weight with 1%.2 % and 3 % (VI'IV) alginate solution concentration and10 11'1210,ml and 30 ml alginate volume. The stability of immobilizedlipase activity for repeated used reactions were also observed The selected immobilized Iipasefromfirst step wasfree:e driedfor 24, 36 and48 hours. Both the hydrolytic activitv and the specific activity of immobilized lipase were affected hv lipase and alginate concentration. The hydrolytic activity of immobilized lipase decreased with the reduction of lipase concentration, however. the specific activity increased accordingly. After freeze drying, the activity of immobilized lipase was also affected by alginate and lipase concentration. The activity of immobilized liposes were stabil, however, increased afterfreeze drying.

EXAMINE ANTIBACTERIA ACTIVITY OF COMMERCIAL FERMENTED DAIRY PRODUCTS AGAINST PATHOGENS BACTERIA Nur Khikmah
Jurnal Penelitian Saintek Vol 20, No 1: April 2015
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

The aim of this research was to examine antibacteria activity of commercial fermented dairy products against pathogens bacteria. The antibacteria activity was determined using the well diffusion method. The presence of antibacteria activity were shown by clear zone which formed around well containing fermented dairy products. The results show that yakult and probiotics yoghurt able to inhibit the growth of pathogens bacteria. The most sensitive pathogen bacteria to fermented dairy products was Salmonella thypii. Inhibition yakult and probiotics yoghurt against gram negative bacteria were greater than gram positive bacteria. Yakult containing Lactobacillus casei strain shirota was able to inhibit all pathogens bacteria, such as Salmonella thypii, Escherichia coli, Bacillus cereus, and Staphylococcus aureus.

DIFFERENCE OF VITAMIN C CONTENT IN PICKLED MANGO FRUIT Sri Palupi
Jurnal Penelitian Saintek Vol 8, No 2: Oktober 2003
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

his research is a survey one which aims at knowing the content of vitamin C of salted mangoes and knowing existances or non-extances of the content of vitamin C of salted mangoes in Jogjakarta Special Region (DIY). Knowing existances or nonextances of the differences of the content of vitamin C of salted mangoes the supply of vitamin C will -be known so that the need of vitamin C for someone can be considered as references. The samples are salted mangoes sold at Mirota Kampus, Gardena and Ramai shop. These shops only sell them every sample was analyzed to find out its water and vitamin C contents using "titrasi yodium" with four time repetitions. These data then was analyzed statistically using two ways of variant analysis. The summary of this research shows that the content of vitamin C every lOO-grama salted mango driedfrom Mirota Kampus has 0,141 gram, Gardena has 0,094 gram. There significant differences of the content of vitamin C of the salted mangoes from the 3 shops. A Null hypothesis (Ho) accepts, p 0,05. It is suggested that people buys the salted mangoes at Mirota Kampus shop because its vitamin C content is higher.

THE UTILIZATION OF GLYCEROL, BIODIESEL SIDE PRODUCT OF USED COOKING OIL AS GLYCEROL ACETATE MATERIAL SYNTHESIS Nelly Fadliyani; Sri Atun
Jurnal Penelitian Saintek Vol 20, No 2: Oktober 2015
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

The research aimed at using glycerol of biodiesel synthesis side product from used cooking oil as glycerol acetate material synthesis, and charactering synthesized material based on data of IR spectroscopy and GCMS. The method used to synthesize glycerol acetate was reﬂ ux during 50 minute at 80 C. Glycerol from biodiesel synthesis side product from used cooking oil was puriﬁ ed with acetic anhydride reacted at 80 0C, for 50 minutes. The synthesis product was characterized by IR and GCMS spectroscopies. The result of this research shows that glycerol derived from synthesizing glycerol 1–monoacetate and glycerol 2-monoacetate in the form of yellow liquid with 145 148 0 0 C boiling point, soluble in ethanol and insoluble in n-hexane. IR spectrum shows the presence of C=O carbonyl, C-O ester, -OH, and C-H aliphatic group; GCMS spectrum shows a mixture from two glycerol monoacetate with SI (Similiar Index) of 97 and 93.

GENETIC DIVISION OF URANIUM RESISTEN BACTERIA AND URANIUM BIOREMEDIATION STRATEGY Bernadetta Octavia; Siti Umniyatie; Aris Bastianudin; L Sembiring
Jurnal Penelitian Saintek Vol 16, No 1: April 2011
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Penelitian tentang keanekaragaman genetik bakteri resisten uranium yang berpotensi dalam strategi bioremediasi uranium telah dilakukan. Pada tahun pertama penelitian multi tahun ini telah berhasil memperoleh 4 isolat bakteri resisten uranium dari limbah uranium fasa organik TBP- kerosin yang berasal dari reaktor riset "Kartini" BATAN Yogyakarta.Metode isolasi diawali dengan metode enrichment dalam medium Glukosa cair dengan penggojokan yang dimaksudkan untuk mengaktifkan kembali metabolisme bakteri aerob dalam sampel limbah uranium. Selanjutnya dilakukan isolasi selektif dengan Quadrant streak method pada medium Nutrien agar yang mengandung uranium dalam bentuk garam uranium yaitu uranil nitrat sebesar 20 ppm. Isolat-isolat bakteri resisten uranium yang diperoleh diseleksi berdasarkan pola pertumbuhan dan pola resistensinya dalam variasi konsentrasi uranium 20, 40, 60, 80, 100 ppm, pada interval waktu 24 jam dengan mengukur optical density masing-masing kultur bakteri pada panjang gelombang 620 nm menggunakan spektrofotometer, Karakterisasi dan identifikasi fenotip dilakukan berdasarkan pengujian karakter morfologi koloni, morfologi sel, sifat fisiologis dan sifat biokimiawi masing-masing isolat bakteri. Hasil karaktersasi dan identifikasi fenotip dianalisis dengan sistematik numerik-fenetik untuk melihat kemiripan di antara isolat bakteri resisten uranium dan genus bakteri acuan.Hasil penelitian menunjukkan bahwa ke 4 isolat bakteri resisten uranium memiliki perbedaan yang cukup signifikan dengan ke 4 genus bakteri acuan karena hanya memiliki level kemiripan 60%. Sedangkan ke 4 isolat bakteri resiten uranium juga menunjukkan perbedaan meskipun memiliki level kemiripan 90%, dan ini berarti bahwa terdapat keanekaragaman isolat bakteri resistan uranium yang diisolasi dari limbah uranium fase organik TBP- kerosin. Berdasarkan pola pertumbuhan dan pola resistensinya maka ke 4 isolat bakteri ini berpotensi untuk dikembangkan dalam program bioremediasi uranium, setelah ditumbuhkan selama 48 jam. Kala kunci: keanekaragaman genetic, resistensi, uranium, bioremediasi

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