cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
mkb.fkunpad@gmail.com
Editorial Address
Gedung Rumah Sakit Pendidikan Unpad/Pamitran Unpad (Teaching Hospital Building) Faculty of Medicine Unpad, 3rd Floor Jl. Prof. Eyckman No. 38 Bandung, 40161, Indonesia
Location
Kota bandung,
Jawa barat
INDONESIA
Majalah Kedokteran Bandung
Published by Universitas Padjadjaran
ISSN : 0126074X     EISSN : 23386223     DOI : 10.15395/mkb
Core Subject : Health,
Medicine & Pharmacology
Majalah Kedokteran Bandung (MKB)/Bandung Medical Journal publishes peer-reviewed original articles and case reports in basic medical research, clinical research, and applied medical science. This journal is published quarterly (March, June, September, and December) by Faculty of Medicine Universitas Padjadjaran. Articles are original research that needs to be disseminated and written in English. All submitted manuscripts will go through the double-blind peer review and editorial review before being granted with acceptance for publication.
Arjuna Subject : -
Articles 651 Documents
 18   19   20   21   22 
Penurunan Aktivitas Tirosinase dan Jumlah Melanin oleh Fraksi Etil Asetat Buah Malaka (Phyllantus emblica) pada Mouse Melanoma B16 Cell-Line Hindritiani, Reti; Dhianawaty, Diah; Sujatno, Muchtan; Sutedja, Endang; Setiawan, -
Majalah Kedokteran Bandung Vol 45, No 2 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.747 KB)

Abstract

Akumulasi melanin menyebabkan hiperpigmentasi dan bila terdapat pada wajah dapat menimbulkan masalah psikososial. Bahan depigmentasi dari tanaman semakin banyak digunakan. Bahan yang dikembangkan harus efektif menghambat sintesis melanin serta tidak toksik terhadap melanosit. Penelitian ini bertujuan menguji efek fraksi etil asetat buah Phyllanthus emblica (P. emblica) atau buah malaka, terhadap sintesis melanin dengan mengukur jumlah melanin dan aktivitas tirosinase, yang merupakan enzim utama sintesis melanin, secara spektrofotometrik pada kultur sel melanosit mouse melanoma B16 cell-line. Sitotoksisitas diukur dengan metode 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Penelitian dilakukan pada November–Desember 2009 di Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, Korea Selatan. Hasil penelitian menunjukkan aktivitas tirosinase dan jumlah melanin menurun bergantung pada dosis perlakuan berbagai konsentrasi fraksi etil asetat buah P. emblica dengan inhibition concentration (IC) 50 berturutturut 95,63 dan 16,90 μg/mL, serta lethal dose (LD) 50 pada konsentrasi 106,64 μg/mL. Simpulan, fraksi etil asetat buah P. emblica mempunyai potensi sebagai bahan depigmentasi, karena mampu menurunkan sintesis melanin melalui inhibisi aktivitas tirosinase. [MKB. 2013;45(2):118–24]Kata kunci: Aktivitas tirosinase, etil asetat, melanin, Phyllanthus emblicaReduction of Tyrosinase Activity and Melanin Amount by Ethyl Acetate Fraction from Malaka (Phyllanthus emblica) Fruit in Mouse Melanoma B16 Cell-LineMelanin accumulation can lead to hyperpigmentation, and if it occurs on the face can cause psychosocial problem. Depigmenting agents derived from plants are increasingly utilized. Agents being developed have to be effective in inhibiting melanin synthesis and should not be toxic to melanocyte. This study aimed was to examine the effect of ethyl acetate fraction from Phyllanthus emblica (P. emblica) fruit, also known as malaka fruit, towards melanine synthesis, which was measured from the melanin amount and tyrosinase activity, the key regulatory enzyme in melanin synthesis, spectrophotometrically towards melanocytes of mouse melanoma B16 cell-line. The cytotoxic effect towards melanocytes was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. This study was conducted on November−December 2009 in Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, South Korea. The result of this study showed that tyrosinase activity and melanin amount decreased in a dose-dependent manner towards various concentrations of ethyl acetate fraction of P. emblica fruit with inhibition concentration (IC) 50=95.63 and 16.90 μg/mL, respectively and lethal dose (LD) 50 concentration 106.64 μg/mL. In conclusion, ethyl acetate fraction of P. emblica fruit is a potential depigmenting agent, since it can reduce melanin synthesis by inhibition of tyrosinase activity. [MKB. 2013;45(2):119–24]Key words: Ethyl acetate, melanin, Phyllanthus emblica, tyrosinase activity DOI: http://dx.doi.org/10.15395/mkb.v45n2.115
Hubungan BRAF V600E dan EGFR dengan Metastasis ke Kelenjar Getah Bening pada Adenokarsinoma Kolorektal Ariyanni, Fenny; Hassan, Abdul Hadi; Hernowo, Bethy S.
Majalah Kedokteran Bandung Vol 47, No 3 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1628.075 KB)

Abstract

Adenokarsinoma kolorektal adalah tumor ganas epitel kolorektal yang berdiferensiasi kelenjar. Metastasis ke kelenjar getah bening sangat memengaruhi prognosis dan penatalaksanaan penderita karsinoma kolorektal. Penelitian ini untuk mengetahui hubungan BRAF V600E dan EGFR dengan metastasis ke kelenjar getah bening. Disain penelitian potong lintang analisis kategorik tidak berpasangan terhadap kasus adenokarsinoma kolorektal yang dipilih secara konsekutif dari blok parafin massa tumor yang dilakukan pemeriksaan imunohistokimia BRAF V600E dan EGFR di Departemen Patologi Anatomi Fakultas Kedokteran Universitas Padjadjaran/RSUP Dr. Hasan Sadikin Bandung pada bulan Februari sampai Juni 2014. Hasil penelitian menunjukkan tidak terdapat hubungan antara imunoekspresi BRAF V600E positif dan metastasis ke kelenjar getah bening dengan p=0,269 (uji chi-kuadrat) dan imunoekspresi EGFR yang positif dengan metastasis ke kelenjar getah bening dengan p=0,713 (uji chi-kuadrat). Imunoekspresi BRAF V600E dan EGFR yang positif tidak berhubungan dengan metastasis ke kelenjar getah bening, p=0,427 (Uji Fisher Exact). BRAF dan EGFR berperan pada epithelial mesencymal transition sehingga sel mampu bermigrasi, tetapi kemampuan migrasi ini tidak berperan pada kejadian metastasis tumor adenokarsinoma kolorektal ke kelenjar getah bening. Simpulan, imunoekspresi BRAF V600E dan EGFR pada adenokarsinoma kolorektal tidak dapat digunakan sebagai petanda agresivitas tumor adenokarsinoma kolorektal. [MKB. 2015;47(3):179–85]Kata kunci: Adenokarsinoma, BRAF V600E, EGFR, kelenjar getah bening, kolorektalAssociation of BRAF V600E and EGFR with Lymph Nodes Metastasis in Colorectal AdenocarcinomaAbstractColorectal adenocarcinoma is an epithelial malignant tumor with glandular differentiation. Lymph node metastasize affects the prognosis and management of colorectal carcinoma patients. In this study, association of BRAF V600E and EGFR with metastasis of the lymph nodes was investigated. This was a cross sectional study with unpaired categorical analysis of colorectal adenocarcinoma obtained from archival paraffin blocks from consecutively selected samples. The blocks were stained by BRAF V600E and EGFR antibody at the Department of Anatomical Pathology, Faculty of MedicineUniversitas Padjadjaran/Dr. Hasan Sadikin General Hospital during the period of February to June 2014. There was no association between positive BRAF V600E immunoexpression  and lymph node metastasis, p=0.269 (p>0.05, chi-square test). Similarly, there was no association between positive EGFR immunoexpression and lymph node metastasis, p=0.713 (p>0.05, chi-square test). Positive BRAF V600E immunoexpresion and positive EGFR immunoexpression also had no association with lymph node metastasis, p=0.427 (Fisher Exact test). BRAF and EGFR may play a role in the epithelial mesencymal transition to increase cell migration and invasion. However, in colorectal adenocarcinoma, BRAF V600E and EGFR were not associated with lymph node metastasis. In conclusions, positive BRAF V600E immunoexpression and positive EGFR immunoexpression in colorectal adenocarcinoma should not be used as markers formetastazing potentials of colorectal adenocarcinoma tumors. [MKB. 2015;47(3):179–85]Key words: Adenocarcinoma, BRAF V600E, colorectal, EGFR, lymph node DOI: 10.15395/mkb.v47n3.600
Gambaran Faktor Risiko Prolaps Organ Panggul Pasca Persalinan Vaginal di Daerah Istimewa Yogyakarta Pangastuti, Nuring; Sari, Dwi Cahyani Ratna; Santoso, Budi Iman; Agustiningsih, Denny; Emilia, Ova
Majalah Kedokteran Bandung Vol 50, No 2 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (677.733 KB) | DOI: 10.15395/mkb.v50n2.1321

Abstract

Prolaps organ panggul merupakan salah satu bentuk disfungsi dasar panggul pada perempuan. Disfungsi dasar panggul adalah keadaan terganggunya fungsi dasar panggul merupakan salah satu kondisi kesehatan yang banyak dikeluhkan perempuan. Lebih dari 46% perempuan dengan riwayat persalinan vaginal mengalami disfungsi dasar panggul,dan peningkatan jumlah paritas berkorelasi dengan peningkatan kejadian prolaps organ panggul. Tujuan penelitian ini adalah untuk mengetahui gambaran karakteristik faktor risiko prolaps organ panggul pasca persalinan vaginal di wilayah Daerah Istimewa Yogyakarta. Penelitian dilakukan pada Januari–Maret 2018. Hasil penelitian didapatkan 51 subjek penelitian dengan persalinan vaginal yang dapat dilakukan pemeriksaan POPQ pada 3 bulan pascapersalinan. Prolaps organ panggul didapatkan pada sebagian besar pasien pascasalin, yaitu sejumlah 46 orang, prolaps uteri 33 orang, sistokel 44 orang, serta rektokel pada 46) orang. Hanya 5 orang yang tidak memiliki gambaran prolaps organ panggul pada 3 bulan pascapersalinan.Dari penelitian ini dapat disimpulkan bahwa faktor risiko prolaps organ panggul pascapersalinan vaginal di Daerah Istimewa Yogyakarta terdiri atas faktor intrinsik (usia ibu, panjang genital hiatus maupun perineal body), dan ekstrinsik (paritas, indeks massa tubuh overweight dan obes, kenaikan berat badan selama hamil >15 kg, serta dilakukan episiotomi dan terjadi robekan perineum). Upaya pencegahan terutama edukasi, perbaikan gaya hidup, pengendalian indeks massa tubuh, pengaturan diet, olahraga penguatan otot dasar panggul, pembatasan jumlah kehamilan dan persalinan, serta pertolongan persalinan sesuai Asuhan persalinan normal. Kata kunci: Disfungsi dasar panggul, faktor risiko, persalinan vaginal, prolaps organ panggul Risk Factors for Pelvic Organ Prolapse in Women with History of Vaginal Delivery in YogyakartaPelvic organ prolapse is one form of pelvic floor dysfunctions in women that impairs the pelvic floor function and also one of the most frequently conditions complained by women. More than 46% women with history of vaginal delivery have experienced pelvic floor dysfunction where higher number of parity correllates to increased pelvic organ prolapse incident. The aim of this study was to understand the  characteristic risk factors of pelvic organ prolapse incident post vaginal delivery in Special Region of Yogyakarta. The study ws performed during the period of January to March 2018 on 51 subjects with history of vaginal delivery evaluated using POPQ in 3 months postpartum. Pelvic floor dysfunction was present in most postpartum patients, i,e, 46 people while 33 experienced uterine prolapse, 44 experienced cystocele, and 46 experienced rectocele. Only 5 subjects had no pelvic floor dysfunction in any form. Risk factors for pelvic organ prolapse in post vaginal delivery in the Special Region of Yogyakarta consist of intrinsic factors (maternal age, genital length of hiatus, and perineal body), and extrinsic factors (parity, overweight status and obese status based on body mass index, weight gain during pregnancy exceeding 15 kg,  episiotomy and the occurrence of perineal rupture). Prevention actions include  education followed by lifestyle improvement, control of body mass index, dietary regulation, exercise, restrictions on the number of pregnancies and childbirth, and safe delivery according to normal birth care standard.Key words: Pelvic floor dysfunction, pelvic organ prolapse, risk factors, vaginal delivery
Perbandingan Efek Musik Klasik Mozart dan Musik Tradisional Gamelan Jawa terhadap Pengurangan Nyeri Persalinan Kala I Fase Aktif pada Nulipara Oktavia, Nike Sari; Gandamiharja, Supriadi; Akbar, Ieva B.
Majalah Kedokteran Bandung Vol 45, No 4 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1210.65 KB)

Abstract

Rasa nyeri persalinan yang kadang luar biasa pada sebagian wanita muncul akibat refleks fisik dan respons psikis ibu. Musik terbukti dapat meningkatkan konsentrasi, mengurangi kecemasan, dan mengalihkan perhatian rasa nyeri yang dialami pasien. Tujuan penelitian ini untuk mengetahui apakah musik klasik Mozart dan tradisional gamelan jawa dapat mengurangi nyeri persalinan, dan apakah musik klasik Mozart lebih baik daripada tradisional gamelan jawa terhadap pengurangan nyeri persalinan kala I fase aktif pada nulipara. Penelitian ini menggunakan metode eksperimen semu dengan total 30 orang nulipara yang berada dalam kala I fase aktif persalinan. Penelitian dilakukan di RSIA Arvita Bunda Kabupaten Sleman Yogyakarta selama Desember 2010–Maret 2011. Subjek penelitian berada dalam 3 kelompok: Mozart (n=10), gamelan jawa (n=10), dan kontrol (n=10). Penilaian nyeri dilakukan dua kali pada masing-masing kelompok dengan numerical rating scale (NRS), yaitu saat ibu berada di awal fase aktif dan di akhir kala I persalinan. Uji analisis statistik menggunakan Wilcoxon Signed Ranks (Z) dan Uji Mann Whitney. Hasil yang didapatkan antara lain, pada kelompok Mozart terdapat nilai median 7,5 (7–10) menjadi 6 (5–9), pada kelompok gamelan jawa median 7 (5–9) menjadi 7,5 (5–10), sementara pada kontrol median 7 (4–9) menjadi 9,5 (6–10). Terdapat perbedaan nyeri antara kelompok Mozart dan kontrol (p=0,001), terdapat perbedaan nyeri antara kelompok gamelan jawa dan kontrol (p=0,022), dan perbandingan antara musik klasik Mozart dan musik tradisional gamelan jawa (p=0,124). Kesimpulan penelitian ini, musik klasik Mozart dan tradisional gamelan jawa mengurangi nyeri persalinan kala I fase aktif pada nulipara, serta tidak ada perbedaan antara keduanya. [MKB. 2013;45(4):218–25]Kata kunci: Musik klasik Mozart, musik tradisional gamelan jawa, nyeri persalinanComparison of Classical Music Mozart Efect and Javanese Gamelan Music Efect to Relief Labor Pain in Stage I Active Phase for NulliparaLabor pain that may sometimes extreme in some pregnant women arises because of the physical reflection and psychological response of the mother. Music is proven to increase concentration, create comfort, and distract the feeling of pain in patients. The aim of this study was to explore wether Mozart classical music and Javanese gamelan music can decrease the level of pain in the process of labor and whether Mozart is better than gamelan in reducing pain in nulipara during stage 1 active phase of labor. This study was conducted by using quasy experiment method with a total of 30 nullipara patients in stage 1 active phase in RSIA Arvita Bunda, Sleman Yogyakarta during the period of December 2010 to March 2011. The subjects were divided into three groups: Mozart (n=10), Javanese gamelan (n=10), and control (n=10). Pain assesment were conducted twice for each group using the numerical rating scale (NRS), which was performed during the beginning of stage I active phase of labor and the end of stage 1 active phase of labor. The data were analyzed using Wilcoxon Signed Ranks (Z) and Mann Whitney statistical tests. The results of this study showed a change in the median value from 7.5 with a range of 7–10 to median 6 (5–9) in Mozar group, from the median value of 7 (5–9) to 7.5 (5–10) in gamelan group and from median 7 (4–9) to 9.5 (6–10) in control group. There was a significant difference between Mozart and control groups (p=0.001), and between Javanese gamelan group and control group (p=0.022), and the comparison between Mozart classical music and Javanese gamelan music (p=0.124). It is concluded that Mozart classical music and Javanese gamelan relief pain in stage 1 active phase of nullipara and no differences are found between the two. [MKB. 2013;45(4):218–25]Key words: Javanese gamelan music, labor pain, Mozart classical music DOI: http://dx.doi.org/10.15395/mkb.v45n4.174
Korelasi Ekspresi Reseptor Vitamin D (VDR) dengan Derajat Diferensiasi dan Stadium Adenokarsinoma Kolorektal Usman, Hermin Aminah; Erida, Yulie; Yulianti, Herry; Hassan, Abdul Hadi
Majalah Kedokteran Bandung Vol 48, No 2 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.125 KB) | DOI: 10.15395/mkb.v48n2.387

Abstract

Keganasan terbanyak ketiga di dunia pada organ kolorektal adalah karsinoma yang berasal dari lapisan epitel mukosa. Modalitas yang digunakan untuk terapi karsinoma kolorektal stadium lanjut selain operasi adalah kemoterapi dan saat ini dikembangkan terapi target sebagai alternatif terapi, yaitu metabolit aktif vitamin D, calcitriol. Calcitriol bekerja sinergis dengan agen kemoterapi yang aktivitasnya dimediasi oleh vitamin D reseptor (VDR). Penelitian ini bertujuan mengetahui korelasi imunoekspresi VDR dengan stadium dan derajat diferensiasi (grading) pada adenokarsinoma kolorektal. Menggunakan 35 blok parafin adenokarsinoma kolorektal yang diambil dari kolektomi, di Departemen Patologi Anatomi Rumah Sakit Dr. Hasan Sadikin Bandung periode 1 Januari 2009–30 Juni 2014, terbagi dalam 3 kelompok stadium (I, II, dan III) dan 3 kelompok derajat diferensiasi (well, moderately, dan poorly differentiated). Imunohistokimia dilakukan pada sampel untuk melihat ekspresi VDR pada massa tumor menggunakan antibodiprimer VDR. Data dianalisis menggunakan uji statistika korelasi Rank Spearman. Terdapat korelasi imunoekspresi VDR dengan stadium (p=0,045; R=-0,341) dan derajat diferensiasi (p=0,000; R=0,558). Pada penelitian ini VDR berperan dalam menentukan stadium dan derajat diferensiasi adenokarsinoma kolorektal. Hal ini dapat dijadikan pertimbangan dalam pemberian calcitriol sebagai bagian dari terapi target pada adenokarsinoma kolorektal dan acuan untuk penelitian selanjutnya dalam perkembangan terapi target. [MKB. 2016;48(2):123–8]Kata kunci: Adenokarsinoma kolorektal, derajat diferensiasi, stadium, imunoekspresi VDRCorrelation of Vitamin D Receptor (VDR) Expression with Differentiation Staging and Grading in Colorectal AdenocarcinomaColorectal carcinoma (CRC) is a malignancy from mucosal epithelium of the colon/rectum. The treatment modalities used for advance stage colorectal carcinoma therapy is chemotherapy, in addition to surgery. Targeted therapy is currently being developed as an alternative therapy. One of the agents used in this therapy is calcitriol. Calcitriol is an active metabolite of vitamin D. Calcitriol works synergistically with chemotherapeutic agents and its activity is mediated by the vitamin D receptor (VDR). VDR plays a role in the inhibition of tumor progression via induction of cellular differentiation and proliferation inhibition. In this study, imunoexpression of vitamin D receptor was examined in conjunction with the staging and degree of differentiation (grading) of colorectal adenocarcinoma. The study objects include 35 colorectal adenocarcinoma paraffin blocks created from colectomy which were collected at the Department of Patology Anatomic of Dr. Hasan Sadikin General Hospital Bandung from January 2009–June 2014. The blocks were divided into three groups of staging (I, II, and III) and  three groups of grading: well, moderately, and poorly differentiated. Immunostaining was used to evaluate the VDR immunoexspression by histo-score. The data were tested using rank spearman test. There was a weak negative correlation between VDR imunoexpression and staging(p=0.045, R=-0.341), and a moderate positive correlation between VDR imunoexpression and grading (p=0.000, R=0.558). It is concluded that a significant correlation of VDR imunoexpression with staging and grading of colorectal adenocarcinoma is found and this finding can be used as a reference for further studies in the development of targeted therapies. [MKB. 2016;48(2):123–8]Key words: Adenocarcinoma colorectal, grading, staging, VDR imunoexpression
Kadar Interleukin-6 Serum pada Karsinoma Payudara Lanjut Lokal dan yang Bermetastasis Sapari, Toha; Abdurahman, Maman; Tjandrawati, Anna
Majalah Kedokteran Bandung Vol 46, No 1 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1312.532 KB)

Abstract

Karsinoma payudara merupakan kanker pada wanita dengan frekuensi tertinggi di dunia dan merupakan penyebab kematian utama. Pasien karsinoma payudara mengalami periode stres fisik dan emosional yang mendorong diproduksinya protein seperti interleukin-6 (IL-6). Interleukin-6 berperan penting dalam tumorigenesis karsinoma payudara melalui aktivasi jalur janus kinase (JAK). Penelitian ini mengukur kadar IL-6 serum yang berperan penting pada patogenesis molekuler proses metastasis pada karsinoma payudara lanjut lokal (KPLL) dibandingkan dengan karsinoma payudara bermetastasis (KPM). Rancangan penelitian adalah potong lintang, analitik komparatif dengan membandingkan kadar IL-6 serum rata-rata pada kedua kelompok KPLL dan KPM. Penelitian dilakukan di Rumah Sakit Dr. Hasan Sadikin Bandung (RSHS) dari Juni–November 2012. Subjek penelitian adalah 35 pasien yang terdiri atas 18 KPLL dan 17 KPM. Kadar IL-6 diukur dengan teknik pemeriksaan enzyme-linked immunosorbent assay (ELISA). Hasil yang diperoleh dari penelitian ini kadar IL-6 serum rata-rata pada KPLL 4,99 pg/mL dan kadar IL-6 serum pada KPM 32,73 pg/mL. Terdapat perbedaan bermakna kadar IL-6 serum antara KPLL dan KPM, p<0,01. Hasil analisis dengan uji chi-kuadrat pada derajat kepercayaan 95%, bahwa terdapat hubungan kadar IL-6 serum dengan kejadian metastasis. Subjek dengan kadar IL-6 serum >9,85 pg/mL memiliki risiko kejadian metastasis 37,5 kali dibandingkan dengan kadar IL-6 serum ≤9,85 pg/mL. Simpulan, kadar IL-6 serum pada KPM lebih tinggi dibandingkan dengan KPLL. [MKB. 2014;46(1):15–21]Kata kunci: Interleukin-6, KPLL, KPMSerum Interleukin-6 Levels in Locally Advanced and Metastatic Breast CancerBreast carcinoma is a cancer in women with the highest frequency in the world and is the leading cause of death. Patients with breast carcinoma experience periods of stress both physical and emotional which encourages them to produce proteins such as interleukin-6 (IL-6). Interleukin-6 plays an important role in tumorigenesis via activation of janus activated kinase (JAK). This study measured the levels of serum IL-6 which plays an important role in the molecular pathogenesis of metastasis in locally advanced breast cancer (LABC) compared to metastatic breast cancer (MBC). The study design was cross-sectional, comparative analytics by comparing the mean levels of IL-6 in both groups: LABC and MBC. The study was conducted at the Dr. Hasan Sadikin Hospital Bandung (RSHS) from June–November 2012. Subjects were 35 women consisting of 18 LABC and 17 MBC. Levels of serum IL-6 were measured by enzyme-linked immunosorbent assay (ELISA) technique. The results of this study show that the mean levels of serum IL-6 on LABC 4.99 pg/mL and the mean levels of serum IL-6 on MBC 32.73 pg/mL. There was a significant difference between the levels of serum IL-6 on LABC and MBC, p<0.01. Results of the analysis with chi-square test shows there is a correlation between level of IL-6 with metastasis incidence. Subjects with levels of serum IL-6>9.85 pg/mL had 37,5 times higher risk for incidence of metastasis. In conclusion, the levels of serum IL-6 on MBCare higher than LABC. [MKB. 2014;46(1):15–21]Key words: Interleukin-6, LABC, MBC DOI: 10.15395/mkb.v46n1.222
Analisis Tipe Staphylococcal Cassette Chromosome mec (SCCmec) Isolat Methicillin Resistant Staphylococcus aureus (MRSA) Sudigdoadi, Sunarjati
Majalah Kedokteran Bandung Vol 42, No 4
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (362.23 KB)

Abstract

Resistensi methicillin resistant Staphylococcus aureus (MRSA) terhadap berbagai antimikrob terutama didasari adanya insersi mobile genetic elements yang disebut Staphylococcal cassette chromosome mec (SCCmec) pada kromosom Staphylococcus aureus. SCCmec tersusun atas gen rekombinase (ccr), gen kompleks mec, gen resisten tambahan, dan insertion sequences. Struktur gen rekombinase memungkinkan SCCmec dapat berpindah dari satu bakteri ke bakteri lainnya. Identifikasi dan analisis SCCmec sangat diperlukan untuk mengetahui dasar genetik resistensi dan memperkirakan penyebaran bakteri ini. Penelitian ini bertujuan menganalisis tipe SCCmec dan hubungannya dengan pola kepekaan MRSA terhadap berbagai antimikrob. Penelitian ini adalah penelitian observasional analitik dengan pendekatan molekuler berupa typing SCCmec dan uji kepekaan terhadap antimikrob, periode Juli–Desember 2007. Pembiakan dan identifikasi 45 isolat MRSA dilakukan di Departemen Mikrobiologi Fakultas Kedokteran Unpad, sedangkan penentuan gen mecA dan PCR multipleks SCCmec dilakukan di Fakultas Kedokteran Universitas Sriwijaya, Palembang. Seluruh 45 isolat MRSA yang diteliti, dipastikan memiliki gen mecA. Analisis PCR multipleks menunjukkan 40 isolat memiliki SCCmec tipe III dan 5 isolat memiliki tipe IV. Semua isolat dengan SCCmec tipe III bersifat multiresisten, sedangkan tipe IV tidak bersifat multiresisten. Dapat disimpulkan bahwa MRSA dengan genotip SCCmec tipe III berhubungan, sedangkan tipe IV tidak berhubungan dengan sifat multiresisten. [MKB. 2010;42(4):149–54].Kata kunci: Methicillin resistant Staphylococcus aureus(MRSA) , pola kepekaan, tipe SCCmecStaphylococcal Casette Chromosome mec (SCCmec) Type Analysis of Methicillin Resistant Staphylococcus aureus (MRSA) IsolatesResistance of methicillin resistant Staphylococcus aureus (MRSA) were based mainly on insertion of mobile genetic elements namely Staphylococcal cassette chromosome mec (SCCmec) in the chromosome of Staphylococcus aureus. SCCmec consists of recombinase genes (ccr), mec genes complex, additional resistance genes, and insertion sequences. Recombinase genes structure mediates transfer of SCCmec from one bacteria to another. Identification of SCCmec is very important to know basic genetic resistance and to predict spreading of MRSA. The aim of this research was to analyze SCCmec type and antimicrobial susceptibility patterns. The design of this study was observational analytic study by typing SCCmec and antimicrobial susceptibility testing on July– December 2007. Isolation and identification of 45 MRSA isolates was performed in the Department of Microbiology, Faculty of Medicine, University of Padjadjaran, whereas identification of mecA gene and typing of SCCmec by multiplex PCR was performed in the Department of Microbiology, Faculty of Medicine, Sriwijaya University, Palembang. The result showed that all isolates contained mecA gene. Multiplex PCR revealed that 40 MRSA isolates had SCCmec type III and 5 isolates with type IV. All SCCmec type III isolates were multiresistant and all of the type IV were not multiresistant. In conclusion, MRSA isolates with SCCmec type III was associated with multiresistant whereas type IV was not. [MKB. 2010;42(4):149–54].Key words: Methicillin resistant Staphylococcus aureus (MRSA), susceptibility patterns, type of SCCmec DOI: http://dx.doi.org/10.15395/mkb.v42n4.28
Hubungan antara Gejala Gangguan Depresi dan Tension-Type Headache (TTH): Studi Eksploratif Setiawan, Cecilia J.; Sadeli, Henny Anggraini; A. Sapiie, Tuti Wahmurti
Majalah Kedokteran Bandung Vol 45, No 1 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (876.068 KB)

Abstract

Prevalensi gangguan depresi semakin meningkat, termasuk yang berkomorbiditas dengan penyakit fisik. Kondisi medik yang telah lama dikaitkan dengan gangguan depresi adalah tension-type headache (TTH). Komorbiditas ini berhubungan dengan perjalanan penyakit TTH kronik. Tujuan penelitian ini untuk mengetahui gejala gangguan depresi yang paling banyak terjadi pada penderita TTH serta menganalisis hubungan antara gejala tersebut dan tipe TTH yang dialaminya. Penelitian ini merupakan studi potong lintang terhadap 32 penderita TTH yang berobat jalan ke Instalasi Rawat Jalan Departemen Ilmu Penyakit Saraf Rumah Sakit Dr. Hasan Sadikin Bandung selama bulan November hingga Desember 2011 yang didiagnosis gangguan depresi. Subjek diperiksa dengan menggunakan instrumen Hamilton Depression Rating Scale (HDRS). Analisis korelasi dilakukan antara skor gejala gangguan depresi yang paling banyak terjadi dengan tipe TTH, dilanjutkan dengan analisis multivariabel untuk mencari rasio prevalens gejala gangguan depresi yang berkorelasi dengan tipe TTH. Angka kejadian gangguan depresi pada TTH didapatkan pada 32/38 penderita, sedangkan gejala gangguan depresi yang paling banyak ditemukan adalah suasana perasaan depresif, kelelahan/berkurangnya minat, dan ansietas psikis. Suasana perasaan depresif dan kelelahan/berkurangnya minat berkorelasi positif dengan tipe TTH (rs=0,411; p=0,019 dan rs=0,379; p=0,032). Dari analisis regresi logistik, hanya suasana perasaan depresif yang meningkatkan risiko TTH kronik, yaitu sebesar 4,74 kali (IK 95% 1,24–18,02). Simpulan, suasana perasaan depresif merupakan gejala yang paling banyak terjadi dan dapat digunakan untuk penapisan dini gangguan depresi pada penderita TTH serta meningkatkan risiko TTHkronik. [MKB. 2013;45(1):28–34]Kata kunci: Gangguan depresi, gejala gangguan depresi, tension-type headacheRelationship between Depressive Disorder Symptoms and the Tension-Type Headache (TTH): An Explorative StudyThe prevalence rate of depressive disorders is increasing, including those having comorbidity with physical illnesses. One of the medical conditions that has been related to depressive disorder is tension-type headache (TTH). This comorbidity is related to the chronic course of TTH. This research aims to know which kind of depressive symptoms are most frequently found in TTH patients and to analyze the correlation between those symptoms and the type of TTH. This was a cross sectional study on 32 TTH patients who visited the outpatient clinic of the Neurology Department of Dr. Hasan Sadikin Hospital Bandung during the period of November to December 2011 and who were diagnosed as having depressive disorder. They were examined using Hamilton Depression Rating Scale (HDRS). We correlated the scores of depressive disorder symptoms with the type of TTH, followed by mutivariable analysis to find the prevalence ratio of depressive disorder symptoms which correlated with the type of TTH. The results showed the prevalence rate of depressive disorder in TTH was 32/38 patients while the most frequent depressive disorder symptoms of the subjects were depressive mood, fatigue and psychological anxiety. Depressive mood and fatigue were positively correlated with the type of TTH (rs=0.411, p=0.019 and rs=0.379, p=0.032). Logistic regression analysis showed that only depressive mood increased the riskof chronic TTH with a prevalence ratio of 4.74 (IK 95% 1.24–18.02). In conclusions, depressive mood, which is the most frequent symptoms of depressive disorder, can be used in the early screening of depressive disorder in TTH patients and this symptom increased the risk of chronic TTH. [MKB. 2013;45(1):28–34]Key words: Depressive disorder, depressive disorder symptoms, tension-type headache DOI: http://dx.doi.org/10.15395/mkb.v45n1.92
Pola Antibodi Antinuklear Sebagai Faktor Risiko Keterlibatan Sistem Hematologi Lupus Eritematosus Sistemik pada Anak Ghrahani, Reni; Setiabudiawan, Budi; Sapartini, Gartika
Majalah Kedokteran Bandung Vol 47, No 2 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.35 KB)

Abstract

Lupus eritematosus sistemik (LES) adalah penyakit autoimun kronik yang melibatkan berbagai sistem organ ditandai dengan produksi berbagai autoantibodi. Penyakit ini memiliki manifestasi klinis yang sangat bervariasi. Antibodi antinuklear diketahui memiliki pola-pola tertentu yang diduga berkorelasi dengan keterlibatan sistem organ tertentu pada LES. Penelitian ini dilakukan untuk melihat hubungan pola antibodi antinuklear (ANA) dengan  keterlibatan berbagai sistem organ pada anak yang menderita LES. Studi potong lintang dilakukan terhadap 93 anak dengan diagnosis LES yang datang ke Departemen Ilmu Kesehatan Anak Fakultas Kedokteran Universitas Padjajaran/Rumah Sakit Dr. Hasan Sadikin Bandung, pada periode September 2006–April 2015. Analisis data dilakukan dengan uji chi kuadrat dan uji-t. Subjek terdiri atas 85 (91%) perempuan dan 8 (9%) laki-laki, dengan rasio perempuan:laki-laki adalah 10,6:1. Usia rata-rata adalah 10,5±3 tahun dan rentang usia 2–17 tahun. Pola ANA terbanyak adalah speckled (58%) dan homogen (19%). Subjek dengan pola ANA homogen lebih berisiko mengalami keterlibatan hematologi yaitu anemia (OR 4,8; IK 95%: 1,1–19) dan leukopenia (OR 3,9; IK 95%: 2,0–7,5) dibanding subjek dengan pola ANA bukan homogen. Tidak didapatkan hubungan pola ANA dengan keterlibatan sistem organ lain. Titer antidsDNA pada subjek dengan pola ANA homogen lebih tinggi dibanding subjek dengan pola ANA bukan homogen (p=0,02). Simpulan, subyek dengan pola ANA homogen memiliki risiko lebih besar mengalami keterlibatan hematologi dibanding dengan pola ANA yang lain. [MKB. 2015;47(2):124–28]Kata kunci: Keterlibatan sistem organ, lupus eritematosus sistemik, pola antibodi antinuklear (ANA)Antinuclear Antibody Pattern as a Risk Factor in Hematological System Involvement in Pediatric Systemic Lupus ErythematosusAbstractSystemic lupus erythematosus (SLE) is a chronic autoimmune disease that can involve any organ system with the evidence of autoantibody production. The disease has a wide range of clinical manifestation. Antinuclear antibody is known to have particular staining patterns and suspected have a correlation with multiorgan involvement. The objective of this study was to define  antinuclear antibody (ANA) staining pattern correlation from multiorgan involvement in 93 children with SLE. This was a cross-sectional study conducted at the Department of Child Health, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital Bandung during the period of September 2006 to April 2015. Data were analyzed using chi-square test and t-test. This study involved 93 children with SLE, consisted of 85 (91%) females and 8 (9%) males, with a ratio of 10.6:1. Mean age was 10.5±3 years with age range of 2 to 17 years. The most frequent ANA staining patterns were speckled (58%) and homogenous (19%). Subjects with homogenous pattern have a higher hematology involvement risk, which are anemia (OR: 4.8, CI 95%, 1.1–19) and leukocytopenia (OR 3.9, 95% CI 2.0–7.5). Subjects with homogenous ANA pattern had a higher titer of anti-dsDNA than those with other patterns (p=0.02). In conclusion, subjects with homogenous pattern have a higher hematology involvement risk. [MKB. 2015;47(2):124–28]Key words: Antinuclear antibody staining pattern, multisystem organ involvement, systemic lupus erythematosus  DOI: 10.15395/mkb.v47n2.571
Peran Terapi Adjuvan Vitamin D3 Terhadap Kadar T Regulator dan Gejala Klinis Penderita Rinitis Alergi Yang Mendapat Imunoterapi Subkutaneus Initial Build Up Dose Sudiro, Melati; Madiadipoera, Teti; Setiabudiawan, Budi; Boesoirie, Thaufiq S.
Majalah Kedokteran Bandung Vol 50, No 2 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (61.23 KB) | DOI: 10.15395/mkb.v50n2.1220

Abstract

Imunoterapi spesifik (ITS) merupakan pilihan terapi pada rinitis alergi yang tidak memberikan respons perbaikan klinis dengan medikamentosa. Peran zat adjuvan diduga dapat meningkatkan efektivitas dan efikasi ITS. Penelitian ini bertujuan menganalisis peran adjuvan vitamin D3 pada ITS terhadap percepatan timbulnya respons imun toleran. Penelitian analitik komparatif numerik dua kelompok tidak berpasangan dengan rancangan uji klinis acak terkontrol ganda ini dilakukan di Klinik Rinologi-Alergi THT-KL RSUP dr. Hasan Sadikin Bandung sejak Mei 2016–April 2017. Sampel terdiri atas 18 pasien menerima ITS+kalsitriol dan 18 pasien menerima ITS+plasebo yang memenuhi kriteria penelitian diambil secara consecutive sampling. Dilakukan pemeriksaan jumlah sel Tregulator dengan flowcitometry pada baseline, minggu ke-8 dan 15 dan skor gejala hidung.  Analisis dengan Uji Mann-Whitney, uji Friedman, uji T berpasangan dan analisis Post hoc. Perbandingan kenaikan rerata median jumlah sel Treg kelompok intervensi dengan kontrol pada minggu ke 8 menunjukkan nilai p=0,04, rerata MFI Treg pada kelompok intervensi dengan kontrol nilai p=0,002. Analisis post hoc terhadap MFI Treg menunjukkan peningkatan pada kelompok intervensi dengan p=0,001 pada minggu ke-8. Disimpulkan perubahan di tingkat imunologi mulai terjadi pada minggu ke-8, tetapi perbaikan gejala klinis terlihat setara.Kata kunci: Imunoterapi spesifik, rinitis alergi, sel t-toleran perifer, sel T-regulator, vitamin D3 Role of Adjuvant Therapy with Vitamin D3 on T-Regulator in Allergic Rhinitis Patient with Subcutaneus Immunotherapy Allergen-specific immunotherapy (AIT) is a potentially disease-modifying therapy that is useful for the treatment of allergic rhinitis, especially in those who do not response to pharmacotherapy. Currently, the role of adjuvant is expected to increase the clinical efficacy in AIT.  The aim of this study was to analyze the role of vitamin D3 adjuvant in AIT to induce immune tolerance. This was an unpaired comparative analytic research with a randomized controlled trial dstudy conducted at the Rhinology-Allergy Clinic ORL-HNS of Dr. Hasan Sadikin General Hospital Bandung from May 2016 to April 2017. Eighteen subjects with AIT+ calcitriol treatment and 18 subjects with AIT+placebo treatment who met the study criteria were selected through consecutive sampling. All subjects were evaluated for Treg cells using flow citometry at baseline, week 8, and week 15. Nasal symptom score was also evaluated.  Statistical analysis performed in this study included Mann-Whitney test, Friedman test, T-Paired test,and Post hoc analysis. There was a significant different of Treg cells average results between the intervention group and control at week 8 with a p-value of 0.04. The mean delta value on MFI Treg reflected significant differences within intervention group (p=0.002). Post hoc analysis results presented a significant increase in MFI Treg in the intervention group at week 8 (p=0,001). Therefore, immunological changes start in week eight but the improvement of clinical symptoms looks similar. Key words: Allergic rhinitis, peripheral t-cell tolerance, regulatory t(treg) cell allergen specific immunotherapy, vitamin D3

Page 20 of 66 | Total Record : 651

 18   19   20   21   22 

Filter by Year

2012 2025


Reset
Filter By Issues
All Issue Vol 57, No 4 (2025) Vol 57, No 3 (2025) Vol 57, No 2 (2025) Vol 57, No 1 (2025) Vol 56, No 4 (2024) Vol 56, No 3 (2024) Vol 56, No 2 (2024) Vol 56, No 1 (2024) Vol 55, No 4 (2023) Vol 55, No 3 (2023) Vol 55, No 2 (2023) Vol 55, No 1 (2023) Vol 54, No 4 (2022) Vol 54, No 3 (2022) Vol 54, No 2 (2022) Vol 54, No 1 (2022) Vol 53, No 4 (2021) Vol 53, No 3 (2021) Vol 53, No 2 (2021) Vol 53, No 1 (2021) Vol 52, No 4 (2020) Vol 52, No 3 (2020) Vol 52, No 2 (2020) Vol 52, No 1 (2020) Vol 51, No 4 (2019) Vol 51, No 3 (2019) Vol 51, No 2 (2019) Vol 51, No 1 (2019) Vol 50, No 4 (2018) Vol 50, No 3 (2018) Vol 50, No 2 (2018) Vol 50, No 1 (2018) Vol 49, No 4 (2017) Vol 49, No 3 (2017) Vol 49, No 2 (2017) Vol 49, No 1 (2017) Vol 48, No 4 (2016) Vol 48, No 3 (2016) Vol 48, No 2 (2016) Vol 48, No 1 (2016) Vol 47, No 4 (2015) Vol 47, No 3 (2015) Vol 47, No 2 (2015) Vol 47, No 1 (2015) Vol 46, No 4 (2014) Vol 46, No 3 (2014) Vol 46, No 2 (2014) Vol 46, No 1 (2014) Vol 45, No 4 (2013) Vol 45, No 3 (2013) Vol 45, No 2 (2013) Vol 45, No 1 (2013) Vol 42, No 3 Vol 44, No 4 (2012) Vol 44, No 3 (2012) Vol 44, No 2 (2012) Vol 44, No 1 (2012) Vol 43, No 4 Vol 43, No 3 Vol 43, No 2 Vol 43, No 1 Vol 42, No 4 Vol 42, No 2 Vol 42, No 1 Vol 41, No 4 Vol 41, No 3 Vol 41, No 2 Vol 41, No 1 More Issue