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BIOTROPIA - The Southeast Asian Journal of Tropical Biology

biotropia
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Published by Southeast Asian Regional Centre for Tropical Biology

ISSN : 02156334 EISSN : 1907770X DOI : http://dx.doi.org/10.11598/btb

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 520 Documents

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Anatomical Characters of Aquilaria microcarpa Interacting with Fusarium sp Siburian, Rima HS; Siregar, Ulfah J.; Siregar, Iskandar Z.; Santoso, Erdy; Wahyudi, Imam
BIOTROPIA Vol. 20 No. 2 (2013): BIOTROPIA Vol. 20 No. 2 December 2013
Publisher : SEAMEO BIOTROP

AbstractAquilaria microcarpa is one of agarwood-producing plants, Interaction of pathogenic fungi may cause physiological changes in plants that have an impact on visual changes in cells, tissues or organs of plants. This study aims to determine differences in the anatomical characters of Aquilaria microcarpa that have been inoculated with Fusarium sp. Observations of anatomical characters showed that traits of inoculated and un-inoculated Aquilaria microcarpa in general are partly different. The difference was found in wood color, odor/aroma, the pores of wood and leather inserted visible. Chemical compounds contained in the plant interact with fusarium was Elemol, Baimuxinal, 3-phenyl-2-butanone, chromen-4-one.

Addition of Essential Oil Source, Amomum Compactum soland ex Maton and Its Effect on Ruminal Feed Fermentation In-Vitro Kurniawati, Asih; Widodo, Widodo; Artama, Wayan Tunas; Yusiati, Lies Mira
BIOTROPIA Vol. 26 No. 3 (2019): BIOTROPIA Vol. 26 No. 3 December 2019
Publisher : SEAMEO BIOTROP

Essential oil (EO), as a feed additive, is known to increase feed efficiency and reduce methane production in ruminants. This research was conducted to study the effect of Java cardamom (Amomum compactum Soland ex Maton) essential oil as a feed additive on ruminal feed fermentation. The in vitro gas production technique was used in this research to determine the effect of cardamom on nutrient digestibility or fermentation in the rumen. Cardamom meal was added into the feed sample to obtain final EO concentrations in the fermentation medium of 0, 25, 50, 75, and 100 mg/L. The substrate consisted of Pennisetum purpureum, rice bran, and wheat pollard. The addition of cardamom did not significantly affect dry matter digestibility, except at 100 mg/L, where it decreased. Protein digestibility decreased when the diet was supplemented with cardamom, whereas organic matter and crude fiber digestibility increased by up to 13.5% and 24% at the 100 mg/L EO level, respectively. The production of volatile fatty acids (acetate, propionate, butyrate), pH, and microbial protein synthesis, except ammonia concentration, were not affected by cardamom addition. Similarly, methane production and protozoa population did not significantly change. The utilization of Java cardamom as a feed additive positively affected ruminal fermentation by increasing organic matter and crude fiber digestibility, while reducing protein digestibility.

GENETIC DIVERSITY ANALYSIS OF THERMOPHILIC BACTERIA FROM CANDRADIMUKA CRATER IN CENTRAL JAVA EMPLOYING PCR-RFLP OF 16S-rRNA GENE DESILIYARNI, TEMMY; SuwANTOAAAo, ANTONIUS; SUHARTONO, MAGGY T.; PURWADARIA, TRESNAWATI
BIOTROPIA No. 14 (1999)
Publisher : SEAMEO BIOTROP

The specific primers for bacteria (63f and 1387r) were used to amplify the 16S-rRNA genes from total community genomic DNA of thermophilic bacteria. The total community genomic DNA was obtained from muds and water samples of Candradimuka crater, Dieng Plateau, Central Java. PCR products were cloned into vectorÂ pCR*2.1-TOPO (3.9 kb) and transformed into Escherichia coli TOPIC. TwoÂ tetrameric restriction endonucleasesÂ RsalÂ andÂ HhalÂ were employed to generate Restriction Fragment Length Polymorphisms (RFLP) paterns. These enzymes yielded 10 and 9 groups of 16S-rRNA profiles or OTU (Operational Taxonomic Units) from 27 16S-rRNA gene clones. Rsal was found to be more discriminative in differentiating the clones than Hhal. Rsal-RFLP indicated that OTU 7 and OTU 3 represented the most abundant clones, i.e. 6 and 5 clones respectively. The distribution of 16S-rRNA gene clones couldÂ indicate relative distribution ofÂ specific groups ofÂ thermophilicÂ bacteriaÂ inÂ theirÂ natural habitat. Analysis of diversity at the DNA level could represent both culturableÂ andÂ unculturable bacteria in theÂ environment. Similarity analysis showed thatÂ at levelÂ 0.600 thereÂ were 8 differentÂ groups from 10Â RFLPÂ profiles generated byÂ RsalÂ digestion. This study indicated that there were at least 8 groups of different thermophilic bacteria occupying Candradimuka crater. Key words: Thermophiles, 16S-rRNA, Candradimuka crater.

Mortality and Ingrowth Pattern of Dipterocarps in Forest Recovery in East Kalimantan Susanty, Farida H.; Suhendang, Endang; Jaya, I Nengah Surati; Kusmana, Cecep
BIOTROPIA Vol. 22 No. 1 (2015): BIOTROPIA Vol. 22 No. 1 June 2015
Publisher : SEAMEO BIOTROP

In primary and logged-over natural foresttrees conditions tree structure, mortality and ingrowth rates , the such as will vary according to the species characteristic. Quantitative management variables become very important to support yield regulation tools for achieving sustainable forest management. The objective was to determine studymortality and ingrowth rates to formulate biometric characteristic variability Dipterocarps forest in logged-over of forests based on time series The was Labanan, East Kalimantan Province. Permanent data.study sitelocated in measurement within -overwere located to represent three, i.e plots logged forest different logging techniques. a) reduced impact logging with diameter limit 50 cm (RIL 50); b RIL 60; c conventional logging; and d) primary forest ) ) as control Total plot permanent area was 48 ha and was measured periodically every 2 years within 17 years after .aboutlogging. For data analysis purpose, trees were divided into Dipterocarps and non-Dipterocarps. two major groups, i.e. Range of mortality rates for all species in logged-over forest were 2.5-29.3% per ha per 2 years which was close very to primary forest at year-5 after logging. While range of ingrowth rate for all species in logged-over forest were 1.3-21.3% per ha per 2 years which were higher than those for the primary forest within 17 years. The mortality and ingrowth rates fluctuation of Dipterocarps species group were different from those of non-Dipterocarps.

POPULATION STRUCTURE OF TOPSHELLS (TROCHUS NILOTICUS) IN SAPARUA ISLAND, CENTRAL MOLUCCAS-INDONESIA Leimena, Handy Erwin Pier; Subahar, Tati S.; Adianto
BIOTROPIA Vol. 14 No. 2 (2007): BIOTROPIA Vol. 14 No. 2 December 2007
Publisher : SEAMEO BIOTROP

Lola snail (Trochus niloticus) is one of the largest sea snails that live in coral reefsat Indo Pacific region and Indonesia including Saparua Island, the province of Moluccas. In a few recent years, the exploitation of lola snails has been increasing, so that the population of lola snails in nature decreases.. The yield of lola snail in Saparua Island and in the archipelagos of Banda from 1979 until 1992 showed a decrease from around 4 tons of dry shells to only around 0.25 tons. Furthermore, this research was aimed to estimate the size structure, age structure, and the sex ratio of lola snails population in Saparua Island, the regency of Central Moluccas. The sampling of lola snails used transects strip method. Â Each transect was 100 m long and 2 m wide, which were divided into 10 segments of 10 m x 2 m. Transect was laid perpendicular to the coast line started from the lowest fall of the tides to the coast. The Bhattacharya method was applied for data analysis. The frequency distribution of shell diameter revealed that lola snails in Saparua Island consisted of nine size classes and two different age classes, which indicated that the population of lola snails in Saparua Island had two periods of spawning. Finally, from this research it could be concluded that the sex ratio of lola snail was 1:1.Â

POTENCY OF VIBRIO ISOLATES FOR BIOCONTROL OF VIBRIOSIS IN TIGER SHRIMP (PENAEUS MONODON) LARVAE WIDANARNI WIDANARNI; A. SUWANTO; SUKENDA SUKENDA; B. W. LAY
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 20 (2003)
Publisher : SEAMEO BIOTROP

This study was carried out to obtain Vibrio isolates able to function as biocontrol of vibriosis in shrimp hatchery. Thirty one Vibrio isolates were isolated from tiger shrimp larvae and hatchery environments, i.e. Labuan, Pangandaran, and Lampung, Indonesia. PathogenicÂ V. harveyi MR5339 was obtained from Maros, South-Sulawesi and was made as a rifampicin resistant mutant (RFR) to screen for those 31 Vibrio isolates in in vitro assays and to allow us to monitor their presence in shrimp larvae and larval rearing water. Almost all VibrioÂ isolates could inhibit the growth of pathogenic V. harveyi MR5339 RFR. SKT-b isolate from Skeletonema was the most effective to inhibit the growth of V. harveyi MR5339 Rf* and significantly reduced larval mortality in pathogen challenge assays. These prospective biocontrol bacteria, at concentration of 10" CFU/ml, did not show pathogenicity to shrimp larvae. SKT-b was Gram negative, short rod-shape, exhibited yellow colonies on TCBS and swarming on SWC-agar media, motile, utilized glucose and sucrose but not lactose: produced extra-cellular protease and amylase, but did not produce chitmase. Partial sequencing of 16S-rRNA gene SKT-b showed SKT-b similarity to Vibrio alginofyticus. Keywords: shrimp larvae / biocontrol bacteria / vibriosis.

Modeling of Tree Growth After Forest Fire in Mount Ciremai National Park, Indonesia Rozak, Andes Hamuraby; Setiadi, Tedi
BIOTROPIA Vol. 23 No. 2 (2016): BIOTROPIA Vol. 23 No. 2 December 2016
Publisher : SEAMEO BIOTROP

Forest fire is a massive threat to tropical forests, causing various negative impacts on nature and human beings. Forest fires often lead to alterations of forest structure and its functions. This study on tree growth after forest fire was conducted using a simulation model. The model was performed at the individual level of the plant community and built to analyze the potential for tree growth and scenarios for post-fire recovery. Five important tree species from the montane forest of Mount Ciremai were chosen to build the model based on four main parameters: plant growth rate, diameter at breast height (DBH), tree-to-grass competition, and tree-to-tree competition. The scenario for post-fire recovery was simulated by replanting similar species with 5 cm DBH seedlings. Predictions from our model showed that most of the chosen species would recover to their pre-fire condition after 37–50 years. Considering the limitations of competition after replanting, it is suggested to minimize tree-to-tree competition and apply silvicultural treatments to maximize tree growth and community recovery.

SULFATE AMMONIUM FERTILIZER ON THE OFF-SEASON PRODUCTION OF SNAKE FRUIT (Salacca sumatrana Becc.) Rasmita - Adelina; irfan Suliansyah; Auzar Syarif; Warnita - -
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 28 No. 2 (2021): BIOTROPIA Vol.28 No.2, Agustus 2021
Publisher : SEAMEO BIOTROP

Sidimpuan snake fruit is one of the local specialties of Padangsidimpuan City in Sumatra. The fruit is known for its sweet, sour and astringent taste which differentiates it from Pondoh and Balinese snake fruit. However, snake fruit farmers have been experiencing an increasing failure rate of fruit-set during the off-season which has led to a decrease in production. Â Â Use fertilization and drip irrigation in the off-season has been suggested as a solution. This research investigates the use of these to overcome the failure of fruit set to optimize production of Sidimpuan snake fruit throughout the year by determining the optimal dosage of ammonium sulfate fertilizer and drip irrigation for fruit set and production in the off-season. This research used a split-plot design with the main plot for drip irrigation and the subplot for ammonium sulfate. Observation parameters include the number of flower and fruit bunches, fruit set percentage and a nutrient analysis of the leaves. The results revealed that drip irrigation had a significant effect on the observed variables of fruit set and number of harvested fruit bunches.Â Drip irrigation had a significant effect on the fruit set percentage and the number of harvested fruit bunches. The best combination of treatments was also discovered to be 400 g ammonium sulfate fertilizer per plant and drip irrigation 3000 ml/plant. The fertilization period of July-Sept gave an off season harvest that was able to match the fruit set percentage (10.76% difference) and number of fruit bunches that were formed (25.65% difference ) by the April-June fertilization for the on-season harvest demonstrating that drip irrigation and ammonium sulfate can overcome fruit set failure in Sidimpuan snake fruit during the off-season.

POLLINATION EFFECTIVENESS OF APIS CERANA FABRICUS AND APIS MELLIFERA LINNAEUS (HYMENOPTERA: APIDAE) IN JATROPHA CURCAS L. (EUPHORBIACEAE) ATMOWIDI, TRI; RIYANTI, PUJI; SUTRISNA, ANDENG
BIOTROPIA Vol. 15 No. 2 (2008): BIOTROPIA Vol. 15 No. 2 December 2008
Publisher : SEAMEO BIOTROP

Pollinators are well known to provide key ecosystem. Animal pollinators are thought to contribute between 15 and 30% of global food production and bees are recognized to be the most important taxon. The pollination effectiveness of two species of bees, Apis cerana and A. mellifera (Hymenoptera: Apidae) in Jatropha curcas (Euphorbiaceae) was studied.Â Three cages, made of insect screen were set up.Â Each cage contains three individual plants. One colony of A. mellifera and A. cerana were placed in the first and second cage, while the third cage was used for control plants. Bee colonies were placed during one month in the first and second cages.Â Â Fruit set of plants pollinated by A. mellifera, A. cerana, control plants, and open plants were counted and compared.Â Pollination by honeybees increased the number of fruits per plant and fruits per raceme of J. curcas. Based on measuring of fruit produced by plants, pollination effectiveness of A. mellifera was higher than A. carana. Key words: Pollination, effectiveness, Apis cerana, A. mellifera, seed set, Jatropha curcas.

LOCALIZATION OF GFDD4-1 EXPRESSED PROTEIN IN Physcomitrella patens CELLS RATNADEWI, DIAH
BIOTROPIA Vol. 13 No. 1 (2006): BIOTROPIA Vol. 13 No. 1 June 2006
Publisher : SEAMEO BIOTROP

The expression of a new dehydration-related gene of Physcomitrella patens, GFDD4-I, was traced for its localization in the plant cells. This revelation is useful to predict the possible roles of the protein in plant tolerance to environmental stress. This gene was fused toÂ gfpÂ marker gene and transfected into the plant protoplasts. Under a confocal laser microscope, it was detected that the GFDD4-1 protein associated with the OFF started to generate at the cell periphery and developed more intensively inwards to cytoplasm, forming vesicles and cystemal structures or network. The protein might be membrane protein which may involve directly in membrane maintenance or cellular protection against stress conditions. Key words : Protoplast transformation, protein localization, dehydration-related gene, GFP, Physcomitrella patens

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Home Page

OAI Link

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Contact Name Knowledge Management Department

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Editorial Address SEAMEO BIOTROP Jl. Raya Tajur Km. 6 Bogor 16134 INDONESIA

Location Kota bogor, Jawa barat INDONESIA

Abstract

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Contact Name
Knowledge Management Department

Contact Email
kmd@biotrop.org

Phone
+62251-8323848

Journal Mail Official
biotropia@biotrop.org

Editorial Address
SEAMEO BIOTROP Jl. Raya Tajur Km. 6 Bogor 16134 INDONESIA

Location
Kota bogor,

Jawa barat

INDONESIA