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The Journal of Experimental Life Sciences (JELS)
Published by Universitas Brawijaya
ISSN : 20872852     EISSN : 23381655     DOI : 10.21776/ub.jels
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 309 Documents
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Plant Growth Promoting Endophytic Bacteria of Coffea canephora and Coffea arabica L. in UB Forest Pratiwi, Esti Rizkiana; Ardyati, Tri; Suharjono, Suharjono
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.07

Abstract

Plant Growth Promoting (PGP) Endophytic bacteria are used as an alternative biofertilizer to support soil health and plant productivity. This research aimed to isolate, analyze the potential, and identify the endophytic bacteria of Robusta and Arabica coffee plants as biofertilizer agents. Endophytic bacteria were isolated from the roots of coffee plants and tested for their potential to produce IAA, phosphate-solubilizing, and nitrogen fixation. Potential endophytic bacterial isolates were identified based on 16S rDNA sequence similarity. Total isolates from Robusta coffee consisting of ten IAA-producing bacteria, eight phosphate-solubilizing, and seven nitrogen fixation bacteria isolates. Total isolates from Arabica coffee roots were 12 isolates of IAA-producing bacteria, seven isolates of phosphate-solubilizing bacteria, and six isolates of nitrogen fixation bacteria. The highest potential of the isolate from Robusta roots was SS.E2 isolate to produce IAA 110.73 μg.mL-1; SS.P3 isolate to dissolve phosphate 4.42 μg.mL-1, and SS.N2 isolate to produce ammonium 3.15 μg.mL-1. The highest potential of the isolate from Arabica roots was SW.E9 isolate to produce IAA up to 257.16 μg.mL-1; SW.P5 isolate to dissolve phosphate up to 4.55 μg.mL-1; and SW.N6 isolate to produce ammonium up to 1.16 μg.mL-1. Isolates SS.E2, SW.E9, SS.P3, SW.P5, SS.N2, and SW.N6 were respectively identified as Bacillus cereus ATCC 14579, Bacillus cereus ATCC 14579, Rahnella aquatilis B35, Kluyvera intermedia TPY16, Rahnella aquatilis B35, and Pseudomonas tolaasii NCPPB 2192. Potential PGP isolates can be developed as biofertilizer agents for the coffee plant. Keywords: Coffee, Endophytic bacteria, IAA, Nitrogen, Phosphate
Differential Intestinal Microbiota Composition Inhibits the Lactobacillus Growth in Rheumatoid Arthritis Patients in Malang, Indonesia. Mufidah, Mufidah; Suyanto, Eko; Sutanti, Viranda; Meidinna, Hazna Noor; Fatchiyah, Fatchiyah
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.02

Abstract

Rheumatoid arthritis (RA) is an autoimmune disease that can cause progressive damage to the joints of patients. The number of patients is expected to increase, along with the exact cause of this disease remains unknown. However, there are several risk factors associated with RA, including dysbiosis. The purpose of this study was to characterize the composition of intestinal microbiota in the RA and control groups through fecal analysis and reveal the association of microbiota composition with RA disease in Indonesia, especially Malang. Fecal samples were obtained from RA patients and controls. Fecal analysis was carried out through several stages, namely the calculation of total bacterial colonies, isolation and characterization of anaerobic bacteria, calculation of the Simpson diversity index, and DNA isolation. Analysis of bacterial composition profiles in fecal was carried out using 6 specific primer sets through PCR analysis. The results of the 16S rRNA PCR analysis showed different microbiota compositions between RA patients and controls. The number of Enterococcus bacterial group was lower in the control patients than the RA group, whereas the Lactobacillus bacteria decreased in RA patients. In addition, our study found that the existence of bacterial isolate 11 changed the composition of microbiota in RA patients, and the DNA band only appeared in Universal primers. The diversity of bacterial species can provide symbiotic and pathogenetic effects in RA patients.Keywords: Dysbiosis, intestinal microbiota, PCR, rheumatoid arthritis.
The Sensitivity of Leersia hexandra Sw. to Gamma-Ray Irradiation Sukmasari, Pritha Kartika; Widoretno, Wahyu; Siswanto, Dian
The Journal of Experimental Life Science Vol. 11 No. 1 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.01.02

Abstract

Gamma-ray irradiation as a physical mutagen has high penetrating power. Therefore, it is most often used to increase genetic variability or produce new mutant plants. This research was conducted to obtain the lethal dose of gamma-rays in Leersia hexandra plants. The used plant part was a single node stolon which had a length of 10 cm with the node in the middle of the stolon. The irradiation doses given were 0, 25, 50, 75, 100 Gy. Stolons were inserted into plastic clips and irradiated using a Cobalt-60 gamma irradiation source at the Center for Irradiation and Radioisotope Applications (PAIR), National Nuclear Energy Agency (BATAN) Jakarta. Gamma-irradiation has a significant effect on inhibiting shoot growth. The growth of irradiated Leersia hexandra with the best number of plants and the highest shoots was obtained at a dose of 25 Gy and decreased with increasing irradiation dose. The lethal dose (LD50) was determined eight days after irradiation using CurveExpert 1.4 software. Leersia hexandra plants that can sprout and regenerate followed the linear equation y = 1.02 - 7.5x with LD50 at 68.85 Gy and LD20 at 29.36 Gy.Keywords: gamma irradiation, genetic variation, Leersia hexandra, lethal dose, mutant plant.
The Genetic and Morphoagronomy Character Diversity of Black Soybean Plant (Glycine soja (L.)): Responses to Mutation Induction by Gamma Rays Irradiation and Ethyl Methane Sulfonate Fauziah, Shaddiqah Munawaroh; Savitri, Evika Sandi; Arumingtyas, Estri Laras
The Journal of Experimental Life Science Vol. 10 No. 3 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.03.02

Abstract

Indonesia has a high dependence on imports of black soybeans. National black soybean needs continue to increase. Therefore, some efforts are needed to increase soybean production. Genetic resources of Black Soybeans with high diversity is also needed to develop superior varieties. One of the ways to increase soybean production is by mutation induction. Therefore, genetic resources of Black Soybeans with high diversity is needed to develop superior varieties. Mutagenesis using gamma rays and EMS can be used to increase genetic diversity. Doses used in this research were 1000 Gy gamma rays, 1% EMS, and combinations. This research was using a completely randomized design with morphoargonomy and molecular parameters. Morphoagronomy parameters observed were plant height, leaf area, seed weight, number of leaves, number of pods, and number of branches. ISSR markers with four primers (UBC 888, ISSR3, UBC876, and UBC889) were used to determine genetic variation of Black Soybeans. Based on the analysis of variance (ANOVA), it was shown that all parameters were able to give an effect on the morphoagronomy of black soybean plants. Gamma rays 1000 Gy were more effective in improving morphoagronomy of the plant in terms of plant height, seed weight, number of leaves, number of pods, leaf area, and number of branches. UBC 888 was the most effective primer to identify the genetic diversity of black soybeans that have been given mutation treatments.   Keywords: Black soybean, EMS, gamma rays, genetic variation, morphoagronomy.
Effect of Cold Storage Time (4°C) on Malondialdehyde (MDA) Level, Motility and Viability Spermatozoa of Cyprinus carpio L. Punten Strain Wirastuti, Rosyi; Marhendra, Agung Pramana Warih; Souhaly, Jantje Wiliem; Rahayu, Sri
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.08

Abstract

The aim of this research is to know the level of MDA, motility, and viability spermatozoa of C. carpio L. in cold storage time at 4°C. This study used treatment extenders that were NaCl 0.9% as control, diluent of egg yolk with concentrate 0%, 5%, 10%, 15%, and storage in 0 h, 24 h, 48 h, 72 h, and 96 h at 4°C. The data were analyzed using ANOVA (P<0.05). The results showed that the quality (motility and viability) of spermatozoa C. carpio L. punten strain in cold storage could be maintained at 96 h. The optimum storage for motility was found in diluent of egg yolk concentrate 5% at 48 h. MDA levels of semen from C. carpio L. Punten strains after being stored at cold temperatures increased at 0 h to 24 h, then decreased after 48 h of storage and increase at 72 h - 96 h stored. Egg yolk concentration and storage time had a significant effect on MDA levels. There is a negative correlation between MDA levels and motility. There was no correlation between MDA levels and the viability of spermatozoa C. carpio L. punten strain. Keywords: C. carpio L, Egg yolk, Malondialdehyde, Motiliy, Viability
Effects of Bitter Melon (Momordica charantia L.) and Starfruit (Averrhoa bilimbi L.) on Proinflammatory Cytokines Produced by Hyperglycemic Mice Model Wardani, Bella Pradina Novinda; Rahayu, Sri; Rifa'i, Muhaimin
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.03

Abstract

Hyperglycemia is a condition of excessive blood glucose in blood plasma caused by damage to the pancreatic beta-cell structure that causes impaired insulin secretion. This study aimed to investigate the combination of Bitter Melon and Star Fruit ethanol extract (SBME) on pro-inflammatory cytokines production in hyperglycemic albino mice models. This study was treated in 25 female albino mice weighing 25-30 and 9 weeks old. STZ was given in a dose of 145 mg.kg-1 BW intraperitoneally. Hyperglycemic mice were given orally with SBME ethanol extract at doses 10, 40, and 160 mg.kg-1 BW for two weeks. After treatment, pro-inflammatory molecules were analyzed by flow cytometry from the splenic cell. This study showed that SBME treatment can reduce the production of pro-inflammatory cytokines especially IL-1b, but increases IL-6 in hyperglycemic mice. After treatment with SBME at a dose of 40, the IL-1b pro-inflammation molecule decreased significantly (p>0,05). It reached a normal physiological level, but the dose has not been able to reduce the IL-6 pro-inflammation molecule significantly. The conclusion of this study was Bitter Melon and Star Fruit ethanol extract (SBME) with a treatment of 40 mg.kg-1 BW can suppress IL-1b pro-inflammatory cytokines in hyperglycemic mice models, but has not been able to reduce the expression of IL-6 pro-inflammatory cytokines. Suggesting this medicinal herb might be a useful strategy for future therapeutic interventions in degenerative diseases or diseases involving cell activation, but a study of doses is needed.Keywords: Averrhoa bilimbi L., Hyperglycemia, Inflammation, Momordica charantia L.
Study of Integrated Pest Management Strategy on The Population of Fruit Flies (Bactrocera spp.) in Red Chili Cultivation (Capsicum Annuum) Muhlison, Wildan; Haryadi, Nanang Tri; Kurnianto, Agung Sih; Ahmada, Bugar Syarif
The Journal of Experimental Life Science Vol. 11 No. 1 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.01.03

Abstract

The fruit fly Bactrocera spp. is the main pest other than Thrips in red chilies, which can reduce plant productivity by 30-60%, so that a specific method of handling this pest is needed. This study examines the application of conventional and Integrated Pest Management (IPM) strategies to fruit fly populations in red chili cultivation (Capsicum annuum). Observation of fruit fly population used the comparative method with methyl eugenol traps and incubation of infected fruit. Determination of the research sample based on purposive sampling method and analyzed using descriptive analysis. The test parameters were the fruit fly population indicated by the host's density and hosts' availability in the applied IPM and conventional treatments. The results showed that the fruit fly species encountered were dominated by B. dorsalis with a percentage of 98.18% and B. carambola 1.82%. The fruit fly population's fluctuation in IPM and conventional treatments were significantly different, as evidenced by a one-way variance test at a significance level of 99%. The population of fruit flies in the IPM concept was 547 flies, while the conventional concept was 1546 flies. The percentage of fruit fly population in red chili plants with IPM treatment was 48% smaller than conventional treatments.Keywords: Bactrocera spp., IPM, Population, Red chilies.
Identification of Sago-Pulp Amylolytic Bacteria and Its Utilization for Granulated Fertilizer Hasanah, Uswatun; Ardyati, Tri; Siswanto, Dian
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.02

Abstract

Previous research stated that bacteria isolated from sago waste from the traditional sago industry in Palopo had the potency to produce amylase. This study aims to confirm the ability of bacteria isolated from sago pulp waste to produce amylase, evaluate the ability of these amylolytic bacteria to produce IAA fix nitrogen, identify selected bacteria, and apply selected bacteria into granules biofertilizers. Bacteria were isolated from sago pulp waste and grown on a 1% starch agar medium. The amylolytic activity was analyzed qualitatively using iodine and quantitatively using the 3,5-Dinitrosalicylic Acid (DNS) method at pH 6. Amylolytic bacteria were analyzed for IAA production using Salkowsky reagent and nitrogen fixation ability through the Serra Kit method. Potential bacteria were identified based on the similarity of the 16S rDNA sequence. The selected bacteria were grown in a 10mL NB medium, and then bacteria were inoculated and air-dried to obtain the granules. The selected bacteria were put into granules and re-isolated using the total plate count (TPC) method. Eight amylolytic bacteria were obtained from sago pulp waste. L1E isolate had the highest amylolytic activity of 1.228 U.mL-1, and L1D isolate had the highest IAA production of 69.8 g.mL-1. Isolate L1E was identified as Alcaligenes faecalis with a 99.45% similarity index, and L1D was identified as Serratia surfactantfaciens with a 99.09% similarity index. Isolate L2G was identified as Alcaligenes aquatilis with a similarity index of 99.8% of the 16S rDNA sequence. The viability of bacteria from granules was 1.41 x 108 CFU.g-1.Keywords: Amylase enzyme, amylolytic bacteria, granulated fertilizer.
The Effectiveness of Weed as Beetle Bank Against Abundance of Soil Arthropods on Corn (Zea mays. L) Sa'adah, Anis; Haryadi, Nanang Tri
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.05

Abstract

Conservation of natural enemies on maize can be done by utilizing grass weeds as Beetle banks. Natural enemies of shelter and get food from the weed grasses that are in the land irrigated corn. Natural enemies observed in this research were the predator of ground arthropods in the form of ground beetles, tomcat, and predators arthropods in other lands. The trap used is the Pitfall trap. The percentage of arthropod predators was 69%, and arthropods herbivore was 31%. The abundance of soil arthropods in the treatment of weeds grass is higher than the control treatment It is because of the treatment of weed grass soil arthropods get habitat for a place to stay. Weed grasses provide food sources for the arthropods and get protection from an environment that is less supportive. The use of weed grasses in addition to Beetle banks also serves as a place to breed. The highest abundance of arthropods was situated on the treatment Eleusine indica that has a lush lawn and has a characteristic odor favored by arthropods. Arthropods that acts as a predator in the land are the family of Formicidae, Philodromidae, Carabidae, Staphylinidae, Pentatomidae. Some Arthropods from the Pentomidae family have a role as a pest and others as natural predators or predators. Arthropods found in the research was an arthropod that had a role as natural enemies or predator. The Pentomidae family that has a position as a predator is Picromerus Bidens. Ground beetles were found in the C. fossor and Pheropsophus sp. Ground beetles are dominant picking weeds Setaria sp. and Eleusine indica as habitat and place in search of food and life's survival. Keywords: Beetle bank, soil arthropods, weeds.
Antimicrobial Activity of Combination Bacteriocin and Asam Sunti Extract (Averrhoa bilimbi L. fermented) Against Multidrug Resistant Escherichia coli in Lettuces (Lactuca sativa) Kimbal, Angie Via Resty; Jatmiko, Yoga Dwi; Ardyati, Tri
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.06

Abstract

The ready-to-eat vegetables are often associated with the presence of multidrug-resistant (MDR) bacteria. This study aimed to evaluate the potency of bacteriocin, Asam Sunti extract, and their combination against MDR E. coli in lettuce. Their antimicrobial activity was assessed using the disk diffusion method and bacterial enumeration after direct application in pre-inoculated lettuce with MDR E. coli. The bacteriocin was produced by Lactobacillus plantarum BP102 at optimum production time or during the stationary phase at 18 h. These bacteriocins were able to inhibit five MDR E. coli isolates, while Asam Sunti extract and the combination of bacteriocin and Asam Sunti extract were only able to inhibit three MDR E. coli (LL1.2, LL3.11, and LL3.12) and (LL1.2, LL1.3, and LL3.11), respectively. In direct application to pre-inoculated fresh lettuce, higher inhibition of MDR E. coli was observed after applying the combination of bacteriocin and Asam Sunti extract with a ratio of 1:1 and 1:2, compared to bacteriocin alone. However, the inhibitory activity of this combination treatment was not significantly different (p>0.05) with the Asam Sunti extract alone. The highest rate of decrease in total bacteria in lettuces was 97% occurred in isolate LL1.2 with bacteriocin treatment alone, and isolate LL3.11 with combination treatment of bacteriocin and Asam Sunti extract (1:2). While on MCA media, the best reduction rate of 94% occurred in isolate LL1.2 with treatment using bacteriocin only, Asam Sunti extract only, and their combination (1:2). The inhibition of MDR E. coli in fresh lettuces by bacteriocin, Asam Sunti extract, and their combination was strain-dependent which was indicated by various inhibition results in all treatments.Key words: Asam Sunti extract, Bacteriocin, multidrug resistant.

Page 21 of 31 | Total Record : 309

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