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The Journal of Experimental Life Sciences (JELS)
Published by Universitas Brawijaya
ISSN : 20872852     EISSN : 23381655     DOI : 10.21776/ub.jels
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 299 Documents
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Analiysis of Phytoplankton Diversity on the Productivity of Vannamei Shrimp (Litopenaeus vannamei) Intensive Pond, Jatisari Village, Banyuwangi Nabilla Anggi Juliyanto; Maftuch Maftuch; Endang Dewi Masithah
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.01

Abstract

Phytoplankton can be used to estimate the potential for vannamei shrimp production. It can be used as a provider of nutrient sources and has an important role in improving water quality. This study aims to analyze the phytoplankton community structure on the productivity of intensive vannamei shrimp ponds. The research was conducted in Jatisari Village, Banyuwangi, East Java, Indonesia. Carried out parameters were the calculation of density, phytoplankton diversity index, culture performance (SR, FCR, ADG), and water quality (temperature, water transparency, salinity, pH, DO, NO2, NO3, PO4, NH4). The results showed that there were six classes and 33 genera from both ponds, Chlorophyceae (10 genera), Bacillariophyceae (8 genera), Cyanophyceae (9 genera), Dinophyceae (3 genera), Euglonophyceae (1 genus), Criptophyceae (1 genus). The index value of the two ponds shows moderate diversity, H 'pond 1 is 1.76, and pond 2 is 2.02. The two plots' cultivation performance was SR 92% and 80%, FCR 1.08 and 1.13, ADG 0.31 g.day-1 and 0.35 g.day-1, respectively. The physical and chemical parameters of the research showed a good enough value for the life of vannamei shrimp and phytoplankton.Keywords: Cultivation performance, Phytoplankton, Vannamei shrimp.
Analysis of Phytoplankton Structure Community, Water Quality and Cultivation Performance in Litopenaeus vannamei Intensive Pond Located in Tembokrejo Village, Muncar, Banyuwangi Gesang Maulana Dwi Katmoko; Yenny Risjani; Endang Dewi Masithah
The Journal of Experimental Life Science Vol. 11 No. 3 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.03.01

Abstract

Phytoplankton plays an important role in Litopenaeus vannamei cultivation. It plays a role as natural feed, water quality control, and indicator of shrimp cultivation success. The community structure of phytoplankton can be influenced by organic matter concentration in ponds. On the other hand, water quality and phytoplankton community structure also influence the productivity of shrimp cultivation. The research aimed to analyze the phytoplankton structure community, cultivation performance and the water quality in one of vananmei shrimp cultivation located in Muncar District. The research used the descriptive method. The research was conducted in two shrimp intensive system ponds in Tembokrejo Village, Muncar District, Banyuwangi, on February-March 2020. Parameters observed were composition, diversity, and density of phytoplankton, water quality parameters, and production performance in each pond. Based on the results, five phytoplankton classes was found: Bacillariophyceae (12 genera), Chlorophyceae (4 genera), Cyanophyceae (8 genera), Dinophyceae (2 genera), and Euglenophyceae (1 genus). Based on the density, Cyclotella and Chlorella were dominated in both ponds. Diversity index values on ponds 1 and ponds 2 were 1.64 and 1.71, respectively. The productivity of both ponds was 10.794 kg.ha-1 and 11.698 kg.ha-1, FCR (Feed Conversion Ratio) were 0.99 and 1.18, and ADG (Average Daily Growth) on both ponds were 0.16 g.day-1. Water quality parameters in both ponds showed an optimal range for vannamei cultivation, except phosphate, which is quite high. Overall, the cultivation performance of both ponds in our research showed good results. However, cultivation performance obtained in this research was not on its best performance yet due to Infectious Myonecrosis (IMNV) infection.Keywords: Cultivation performance, Litopenaeus vannamei, muncar, phytoplankton.
Preventive effects of yogurt fortified with purple roselle extract against cardiotoxicity in rats exposed with 2,3,7,8-tetrachlorodibenzo-p-dioxin Ani Setianingrum; Iffa Fanadila; Herlina Pratiwi; Ajeng Erika Prihastuti Haskito
The Journal of Experimental Life Science Vol. 11 No. 3 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.03.02

Abstract

Dioxin is one of the environmental persistent organic pollutants that harm animal and human health. Prolonged exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) can disrupt normal organ function including heart. Yogurt is known to have antioxidant properties, and fortification natural plant-based antioxidant purple roselle (Hibiscus sabdariffa L) extract into yogurt can improve the antioxidant potential. The purpose of this study was to determine the preventive effect of yogurt fortified with purple roselle on dioxin exposure rats (Rattus norvegicus) based on the levels of Malondialdehyde (MDA) and histopathological of the heart. This experimental study using a completely randomized design (CRD). A total of 30 male Wistar rats were divided into 5 groups (NC, PC, T1, T2, T3), a dose of 200 ng/kg BW TCDD and 1 mL of roselle yogurt 0.5%, 1.0%, and 1.5% were given orally for 12 days. MDA levels were analyzed using the Thiobarbituric Acid (TBA) assay and histopathology of the heart using the Haematoxylin Eosin (HE) staining method. Statistical analysis using one-way Analysis of Variance (ANOVA). Histopathology changes were analyzed descriptively. The results showed that TCDD exposure induced oxidative stress in heart tissue and yogurt roselle has the potential to prevent an increase of MDA levels significantly (p<0.05). Furthermore, TCDD can cause histopathological alterations such as necrosis and hemorrhage, and yogurt roselle was proven to prevent cardiotoxicity due to TCDD exposure.
Dynamical Analysis and Parameter Estimation of Hepatitis B Disease Model in Malang Fitroh Aulani; Wuryansari Muharini Kusumawinahyu; Isnani Darti
The Journal of Experimental Life Science Vol. 11 No. 3 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.03.03

Abstract

In this article, a model representing the spread of Hepatitis B disease is constructed as a nonlinear autonomous system. The model divides the considered human population into three classes, namely susceptible, infected, and recovered class. The dynamical analysis shows that there are two equilibrium points in the model, namely a disease-free equilibrium point and an endemic equilibrium point. The existence and stability of the equilibrium points depend on the basic reproduction number (R_0). The disease-free equilibrium point is local asymptotically stable when R_0<1 while the endemic equilibrium point exists and is local asymptotically stable if R_0>1. The five parameters of the model are estimated by applying Downhill Simplex (Nelder-Mead) Algorithm and by using the infected data cases taken from such a hospital in Malang. The estimated parameters are the transmission of infection rate, the saturation rate, the vaccination rate, the recovery rate, and the immunity loss rate. The resulting parameter estimation supports the analytical result and is used to illustrate the analytical results numerically. Based on the considered model and the result of the parameters estimation, it can be concluded that the Hepatitis B spread in Malang is controllable. Keywords: downhill simplex (Nelder-mead) algorithm, dynamical analysis, hepatitis B model, parameter estimation. 
In Silico Study to Predict the Potential of Beta Asarone, Methyl Piperonylketone, Coumaric Acid in Piper Crocatum as Anticancer Agents Ahmed Hasan Abkar; Moch. Sasmito Djati; Widodo Widodo
The Journal of Experimental Life Science Vol. 11 No. 3 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.03.04

Abstract

Piper Crocatum Ruiz & Pav leaves often be used to treat various diseases, including cancer, empirically. This study aimed to analyze the anticancer activities of Piper Crocatum bioactive compounds via In Silico analysis. The methods were biological activity analysis, cell line cytotoxicity activity, SwissADME, STITCH, molecular docking, and molecular dynamics simulation. The investigated bioactive compounds were β-asarone, methyl piperonylketone, and coumaric acid. The results showed the biological activities of the compounds related to anticancer were anti-mutagenic, TNF expression inhibitive, and MMP9 expression inhibitive. The prediction of cytotoxicity analysis results showed that investigated bioactive compounds were toxic on various tumor cell lines. Based on swissADME results, almost all compounds have good pharmacological properties, except coumaric acid. Docking analysis demonstrated the presence of bioactive compounds inhibited TNFa, HER2, and MMP9 as the target protein. The molecular dynamic result was confirmed using molecular dynamic and it shows that β-asarone interaction was stable against MMP9 and TNF protein showed by the low RMSD value. The study found the investigated bioactive compounds of Piper crocatum have the activity of anti-cancer via the inhibition of TNFa and MMP9 protein. However, further research still needs to be done to confirm the prediction results of this In Silico study. Keywords: anticancer, In Silico, molecular docking, Piper crocatum.
Expression Virus-Like Particles (VLPs) at Geomembrane and Concret in Asian Pacific Shrimp Culture (Litopenaeus vannamei) Venny Nur Hidayah; Muhammad Musa; Yuni Kilawati
The Journal of Experimental Life Science Vol. 11 No. 3 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.03.06

Abstract

Asian Pacific Shrimp is an invertebrate that the most culture in aquaculture. But the high demand for shrimp makes farmers cultivate a high density. There are several ways to increase the density of shrimp culture, usually called geomembrane plastic and concrete ponds. The ponds are located in the Laboratory of Brackishwater and Seawater Fisheries, Probolinggo and Lucky Windu, Situbondo. Day of Culture (DOC) on Laboratory of Brackishwater and Seawater Fisheries in Probolinggo pond is 85 days both, while in Lucky Windu Situbondo is 81 days on pond 2, and pond  3B 133 days. The study aimed to evaluate correlations of VLPs towards geomembrane and concrete ponds. The research method has two observations: 1. Periodic observations; nitrite, ammonium, and shrimp were observed; 2. Last observation; sampling water for analysis VLPs was observed. Data of sampling water in the different ponds were then analyzed using Pearson correlation analysis to look for the level of tightness and direction of the relationship. The results have obtained that geomembrane VLPs and concrete VLPs has a very strong relationship, while the type of geomembrane and concrete was close to 1 > 0.5 and the result is 0.90, 0.96, 0.69, and 0.82. Water quality showed pH 8.1 at geomembrane, nitrite 0.2mg.L-1 at geomembrane, and ammonium of 0.44 mg.L-1 at concrete. The pond area in geomembrane pond 1 and 2 was 1600 m2, meanwhile in concrete both is 4300 m2, which mean in concrete ponds more the existence of VLPs is because due to the different pond area factors and indicates from detections of WSSV that the result was positive that makes environment factor in abundances VLPs. But VLPs have no effect on the growth of shrimp vaname during the cultivated process.Keywords: Asian Shrimp Pasific (L. vannamei), Virus-Like Particles (VLPs), Water Quality, and WSSV.
Screening of Potential Biosurfactant Producing Bacteria from Tanjung Perak Port, Surabaya Marlinda Elvina Susanti; Maftuch Maftuch; Asep Awaludin Prihanto
The Journal of Experimental Life Science Vol. 11 No. 3 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.03.05

Abstract

Bacteria are bioremediation agents that are advised to overcome water pollution. This study aims to isolate biosurfactant-producing bacteria for bioremediation from Tanjung Perak Port, Surabaya. The isolates grown in specific media were tested for drop collapse, oil spreading test, and emulsification index. A total of 12 bacterial isolates were isolated from Tanjung Perak seawater contaminated with diesel oil. In the drop collapse test, four isolates had the highest positive score from this test, isolate LU-5, LU-7, LU-9, and LU-11. While in the second test, the oil spreading test, the highest positive score results were obtained in three isolates, isolates LU-2, LU-7, and LU-11. The emulsification index (E24) value showed that isolates LU-7, LU-9, and LU-11 were significantly higher than the others, which was above 10%. This study concludes that in Tanjung Perak, seawater-contaminated oil isolated several bacteria that produce biosurfactants, which have the potential to be developed as bioremediation agents.Keywords: bacteria, bioremediation, biosurfactant, diesel oil
Phytoplankton and Its Relationship to White Leg Shrimp (Litopenaeus vannamei) Culture Productivity in Alasbulu, Banyuwangi Hasna Kamilia; Bambang Budi Sasmito; Endang Dewi Masithah
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.03

Abstract

Shrimp culture is related to phytoplankton's existence as a primary producer and water quality. Aside from their function as natural feed, phytoplankton has a role in maintaining the stability of the pond ecosystem. It is indicated by the high abundance and diversity of phytoplankton, as well as suitable water quality to support the productivity of shrimp culture. This research aimed to determine the correlation between phytoplankton structure community, water quality parameters, and shrimp productivity. This research used a descriptive method. This research was conducted in shrimp culture intensive system Alasbulu Village, Wongsorejo District, Banyuwangi on February-March 2020. The parameters observed are diversity and abundance of phytoplankton, water quality parameters, and production performance in each pond. Based on the results, six phytoplankton classes were identified: Bacillariophyceae (10 genera), Cyanophyceae (8 genera), Chlorophyceae (5 genera), Dinophyceae (2 genera), Euglenophyceae (1 genus), and Cryptophyceae (1 genus). Chlorophyceae dominated both ponds, followed by Cyanophyceae. Diversity index values on ponds 1 and ponds 2 were 1.39 and 1.50, respectively. Productivity of both ponds were 1.8 kg.m-2 and 1.4 kg.m-2; FCR (Feed Convertion Ratio) 1.1 and 1.3; ADG (Average Daily Growth) 0.3 g.day-1 and 0.25 g.day-1. It can be concluded from this research that high density of Chlorophyceae in phytoplankton community is one of the main causes that supported shrimp cultivation. Keywords: Banyuwangi, Litopenaeus vannamei, phytoplankton, productivity. 
Effect of Prebiotic and Probiotic Fish Feed on Physical, Chemical and Biological Quality of Feed M. Indra Wahyu Pratama; Anik Martinah; Ating Yuniarti
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.04

Abstract

The limitations of fish in digesting food depend on the presence of enzymes protease, amylase, and lipase that react with substrates in the digestive channel of fish. Supplementation methods can be performed to increase fish growth by adding prebiotics and probiotics to the feed. This study aims to determine the influence of the difference in the length of time fermented prebiotic feed (sweet potato extract) and probiotics (Bacillus megaterium) on the quality of feed. The results of the physical analysis of fish feed showed the color looks brown, fishy smell, texture, and general conditions of feed seemed completely normal. Chemical analysis showed that the proteins were best improved in B3 treatment, with the period of fermentation approximately 72 hours. Biological analysis suggests that the longer the fermentation time taken, the higher the abundance of bacteria obtained.Keywords: B. megaterium, chemistry and biology of fish feed, physical analysis, sweet potatoes (Ipomoea batatas L)
Isolation and Characterization of α-Amylase Enzyme on Brown Planthopper (Nilaparvata lugens Stal) On Rice Syakuro, Muhammad Yusuf Abdan; Haryadi, Nanang; Muhlison, Wildan; Sucipto, Irwanto
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.02

Abstract

Brown leafhopper (Nilaparvata lugens Stal) is an important pest in Indonesia that causes heavy losses. The rice was damaged by N. Lugens sucking plant liquid in the form of carbohydrates in the form of starch. Environmentally friendly control methods are needed to reduce the increasingly high use of inorganic pesticides. Control of N. lugens with biotechnology can be done by inhibiting the metabolic cycle in these insects. This method utilizes the proteins available in rice seeds to stop the performance of enzyme α-amylase in the digestive system of N. lugens. α-amylase is an enzyme that plays a role in the process of starch degradation so that it becomes a simpler form both in microorganisms. The characterization of α-amylase enzymes in insects is an important first step to the determination of appropriate protein inhibitors so that they can be used to produce pest-resistant Genetically Modified Organisms crops. The research was conducted in the Agrotechnology laboratory, University of Jember. The research began with isolation and purification of α enzymes from brown stems and then continued with testing of α-amylase activity. Test parameters include the effect of temperature, pH, and substrate concentration on the activity of the α-amylase enzyme. The results showed that supernatant extracted from brown planthoppers produces a clear zone in the agar medium, which means the activity of enzymes α-amylase in the hydrolysis of starch. The pH value of 6 provides the most optimum conditions for the activity of α enzymes. The α-amylase enzyme is able to work optimally in the temperature range of 30°C - 45°C, and experiences a decrease in activity when the temperature reaches 50°C. The α-amylase enzyme shows the ability to hydrolyze the amylase substrate to a concentration of 0.8 μg.μL-1. Keywords: α-amylase, Nilaparvata lugens Stal, Rice.

Page 22 of 30 | Total Record : 299

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