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The Journal of Experimental Life Sciences (JELS)
Published by Universitas Brawijaya
ISSN : 20872852     EISSN : 23381655     DOI : 10.21776/ub.jels
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 299 Documents
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Microbial Agents in Terrorism, Biomarkers, and Public Health Challenges Orole, Olukayode; Chuku, Aleruchi; Olawuyi, Kayode; Mantu, Eno; Philips, Alexander; Okoli, Chukwuma
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.09

Abstract

Bioterrorism agents are mostly microorganisms with the capacity to deal explosive and lethal harm to humans, animals, and food crops. These microorganisms spread in the form of gases, whole organisms, or products of secondary metabolism of microorganisms. Classification of the agents is into three categories based on the ease of dissemination and end effects on a living population. While most health institutions are equipped to take care of sick people and treat suspected cases of infections, these institutions lack basic means of identifying bioterrorism acts. Special diagnostic equipment to identify causal organisms or agents is not available. Lack of training on what to do when terrorists strike using biological agents can cumulatively increase the lethal effects of such agents. Molecular techniques of identifying microorganisms to species level are as promising as they are time-consuming, while technical expertise and a conducive environment for managing such equipment are mostly not available in the African setting. The governments in Africa as a matter of urgency should provide an atmosphere where the teaming population of people without jobs are employed, while hospitals are adequately equipped, and training of health workers on what to do immediately after cases of terrorism are reported. The review highlights these agents and the diagnostic tools necessary to facilitate early response to bioterrorism. Keywords: Bioagents, Bioterrorism, Classification, Identification, Spread.
Isolation and Identification of Nitrogen-Fixing Rhizobacteria associated with Cocoa plantation (Theobroma cacao L) as Biofertilizer Agent Nurul Afiyatul Jannah; Mustafa, Irfan; Jatmiko, Yoga Dwi
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.01

Abstract

Ringinkembar Village, Sumbermanjing Wetan District, Malang Regency, is one of the centers for cocoa plantations using an organic farming system. However, over time this organic farming system experienced a decrease in fruit production, possibly from soil fertility and biofertilizers that were less available in the soil. This study aims to analyze the nitrogen-fixing ability and identify rhizosphere isolates that excel in nitrogen-fixing obtained from the rhizosphere of cacao (Theobroma cacao L) plant. Bacteria were isolated from the soil surrounding cocoa plant roots and grown on Nfb (Nitrogen free Bromothymol Blue) agar media. The nitrogen-fixing bacteria were analyzed with quantitative and qualitative methods. Six potential nitrogen-fixing isolates were identified based on the 16S rDNA sequence. The total number of isolates obtained from nitrogen-fixing isolation was six isolates. The R3.FN1 isolate showed the highest ammonia index at 0.52 µg.L-1 and was identified as Stenotrophomonas maltophilia KB13 with 99.87% similarity to the 16S rDNA sequence. Keywords: Cocoa, Nitrogen-fixing, Rhizosphere, Stenotrophomonas maltophilia.
Curcuma mangga Ethanol Extract Improves Sperm Quality of Mice Exposed to Monosodium Glutamate Silvia Aini; Marhendra , Agung Pramana Warih; Rahayu, Sri
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.04

Abstract

The current study aimed to determine variations effect in the dose of Curcuma mangga ethanol extract on male mice exposed to monosodium glutamate (MSG). The 24 adult male mice with the body weight of 25-30 g and 3-4 months old were used, then divided into six groups (n= four per group). The groups are K- (untreated mice), K+ (induced MSG) for 45 days, P1 (MSG + C. mangga extract 25 mg.g-1 BW), P2 (MSG + C. mangga extract 50 mg.g-1 BW); P3 (MSG + C. mangga extract 75 mg.g-1 BW); and P5 (MSG + C. mangga extract 100 mg.g-1 BW) for 30 days. Animals were dissected on the 46th day. The epididymis was isolated, and sperm quality was evaluated. The results showed that25-75 mg.g-1 BW C. mangga ethanolic extract could increase sperm viability by 24-29%, while at a dose of 25-100 mg.g-1 BW the sperm motility decreased by 58-92%. The administration of 100 mg.g-1 BW C. mangga could decrease it by 58% and concentration at a dose of 100 mg.g-1 BW increased sperm concentration by 96%. The use of MSG can increase sperm abnormalities compared to controls. This study showed that C. mangga effectively improved the sperm quality of mice exposed to MSG. Keywords: Curcuma manga, Epididymis, Monosodium Glutamate, Sperm quality.
Anti-inflammatory Activity of Elephantopus scaber and Sauropus androgynus Combination in Pregnant Mice Infected with Escherichia coli Asfi, Nida; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Rifa’i, Muhaimin; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.06

Abstract

The current study aimed to investigate the effectiveness of Elephantopus scaber and Sauropus androgynus as anti-inflammatory agents in pregnant mice infected with Escherichia coli. This study used seven treatments group (n=3): K- (healthy pregnant mice), K+ (pregnant mice infected with E. coli), P1 (pregnant mice infected with E. coli + E. scaber 100%), P2 (pregnant mice infected with E. coli + E. scaber 75% and S. androgynus 25%), P3 (pregnant mice infected with E. coli + E. scaber 50% and S. androgynus 50%), P4 (pregnant mice infected with E. coli + E. scaber 25% and S. androgynus 75%), P5 (pregnant mice infected with E. coli + S. androgynus 100%). Flow cytometry analysis was used to analyze cell populations expressing CD4+TNFα+, CD4+IFNγ+ and regulatory T cells (CD4+CD25+CD62L+). All treatment groups significantly (p<0.05) decreased TNFα and IFNγ levels, while the P2 group was more effective in increasing regulatory T cells at the 1st and 2nd trimesters of the pregnancy. This study showed that E. scaber and S. androgynus combination alleviated inflammation by reducing inflammatory cytokines (TNFα and IFNγ) and increasing T-regulatory cells. Therefore, E. scaber and S. androgynus combination could suppress the inflammation during pregnancy and infection. Keywords: Elephantopus scaber, immune system, infection, inflammation, Sauropus androgynous.
Aquatic Environmental Analysis of the Hematological Profile of Barbonymus altus in the Brantas River, Jombang, East Java Aang Setyawan Anjasmara; Hertika, Asus Maizar Suryanto; Yanuhar, Uun
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.07

Abstract

The Brantas River is the longest in East Java. One of the areas included in the flow of the Brantas River is the Jombang Regency. The Jombang Brantas River is used by the community to meet their daily needs in agriculture, plantations, industry, and fishing grounds. The input of waste generated from several community activities will result in the survival of fish in the waters. Fish as a biomarker agent is quite significant in estimating the level of heavy metal pollution. Red Bader Fish (Barbonymus altus) is often found and lives around the Jombang Brantas River. This study aims to determine the condition of the aquatic environment by observing the health of the fish through hematological observations of the Red Bader Fish (B. altus). Sampling was carried out at three stations with three replications every two weeks, which was carried out in February – March 2022 in the Jombang Brantas River. Data analysis used the Canonical Correspondence  Analysis (CCA) method as an analytical method to determine the correlation between the dependent variable (hematological profile) and the independent variable (water quality conditions). In this study, the results showed that the water quality at Station 1 and Station 2 was classified as normal, while at Station 3, it was classified as polluted. Data analysis showed that erythrocytes, hemoglobin, and hematocrit had a relationship with temperature, pH, DO, BOD, ammonia, TSS, and TDS in low concentrations. Meanwhile, leukocytes and micronuclei showed a relationship with high concentrations of ammonia, BOD, TDS, and TSS. Keywords: Brantas River, Bader fish (Barbonymus altus), CCA, hematology, water quality.
Isolation and Screening of Lactic Acid Bacteria From Sumbawa Buffalo Milk (Bubalus bubalis) as Potential Starter Cultures Harmoko, Deni; Ardyati, Tri; Jatmiko, Yoga Dwi
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.03

Abstract

The Sumbawa buffalo (Bubalus bubalis) is one of the ruminant livestock in Indonesia that not only contributes to fulfilling meat requirements but also milk. Besides containing nutrients that are very beneficial for human health, buffalo milk is also a potential source of lactic acid bacteria (LAB) with technological and functional properties. Lactic acid bacteria have been utilized as starter cultures in various fermented products. This study aimed to isolate LAB from Sumbawa buffalo milk and to identify the potential isolate as a starter culture. The screening of LAB as a starter culture was based on some technological properties, including proteolytic activity, lipolytic activity, exopolysaccharide (EPS) production, antibacterial activity, antibiotic sensitivity, hemolytic activity, and acidification activity Data were analyzed statistically using one-way ANOVA and Tukey’s post hoc test at a 5% significance level. A total of 21 isolates were isolated from fresh buffalo milk, with a LAB total was 4.7x105 CFU.mL-1. All the isolates were characterized as Gram-positive with cocci-shaped. The SA8 isolate was selected as the most potential candidate as a starter culture because it has fulfilled the criteria such as the highest proteolytic activity, the lowest lipolytic activity, producing EPS, potential antagonistic activity against Bacillus cereus, Escherichia coli, and Salmonella Typhi, and sensitivity to cefazolin, intermediate to erythromycin and cinoxacin, non-pathogen, as well as the most rapid acidification activity. The SA8 isolate was identified as Enterococcus lactis with a similarity level of 99.99% towards strain BT159. This indigenous LAB was a potential starter culture of Sumbawa fermented buffalo milk to increase the diversification of products derived from buffalo milk. Keywords: Enterococcus lactis, lactic acid bacteria, technological properties, starter culture, Sumbawa buffalo milk.
Textile Industry Waste Pollution in the Konto River: A Comparison of Public Perceptions and Water Quality Data Adjid, Galang Ayuz Firstian Adjid; Kurniawan, Andi; Nazriati, Nazriati
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.05

Abstract

Textile industry production activities in one of the Badas areas can have implications for the occurrence of dye liquid waste pollution in the Konto river flow. This implication leads to the public perception that there has been river pollution from textile dyeing liquid waste. In this case, public perceptions need to be analyzed as a form of river environmental monitoring activities. Therefore, public perceptions of pollution were compared with actual water quality data, especially related to dyes in river water, to link public perceptions with environmental resource management efforts. This study aims to analyze public perceptions of textile dye waste pollution and compare the analysis results with the river water quality analysis. Analysis of river water quality (dye content, BOD, COD) was conducted at five sampling points in Badas, Kuwik, Balungjeruk, and Wonorejo villages. Public perception was measured descriptively through interviews with respondents referring to the Slovin method. The analysis of public perceptions shows that the Public considers that there has been pollution of river water, mainly due to textile industry waste, along the Konto River. The results of the water quality analysis showed that dye concentrations were found at four sampling points except for Wonorejo Village. This result shows that the correlation between public perception and data on dye contamination only occurs in Badas, Kuwik, and Balungjeruk villages. In addition, the BOD/COD ratio indicates that pollution has occurred at all observation points in this study. Keywords:  Aquatic Ecosystem, Dyes, Pollution, Public Perception, Waste.
Exploring YidR Gene from Klebsiella pneumoniae To Design a Multi-epitope Vaccine Using Immunoinformatics Approach Wildan Seputra, Maulana; Susiati, Hani; Raras, Tri Yudani Mardining
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.08

Abstract

Klebsiella pneumoniae is the main leading cause of hospital-acquired infection, leading to severe infections such as pneumonia and urinary tract infection. There is an urge to develop an efficient Klebsiella vaccine, and epitope-based vaccines may offer a practical choice. Therefore, this study aims to design an epitope-based vaccine construct. YidR gene is expressed in all strains of Klebsiella with 97.6% of sequence homology, which makes it a potential vaccine candidate. In silico approach was used to make a multi-epitope vaccine construct consisting of immunogenic epitopes against the YidR gene of Klebsiella. Acquired multi-epitopes vaccine was analyzed for antigenicity, allergenicity, toxicity, and various physicochemical parameters. Molecular docking simulation was performed using TLR2 and TLR4 to asses binding affinity with the designed vaccine construct. The results showed that the predicted multi-epitope-based vaccine candidate is non-allergen, non-toxic, and has an efficient binding affinity towards TLR2 and TLR4 that could effectively induce immune responses. Keywords: In Silico, Klebsiella pneumoniae, vaccine design, YidR gene.
The Relationship of Chemical Compounds and Crystal Development in Porang Tuber (Amorphophallus muelleri Blume) Chairiyah, Nurul; Harijati, Nunung; Mastuti, Retno
The Journal of Experimental Life Science Vol. 13 No. 1 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.01.01

Abstract

Chemical compounds are presumed to interact with each other and potentially affect the crystal development of porang tubers. This study focused on glucomannan and calcium oxalate (CaOx) as chemical compounds found in porang. Crystal development was analyzed by microscopic observation. The tuber tissue was used for microscopic slides and was harvested two weeks before the plants lost their leaves, when the plants lost their leaves, and two weeks after the plants lost their leaves. The CaOx and glucomannan content was measured using a modified extraction method. The increasing of CaOx content tends to increase the number of CaOx crystals. The analysis showed that the crystal density could affect the increasing of tuber weight possibility. There was a development process in raphide crystals that showed by different sizes at three harvest times, which the others were not. The results also indicated that glucomannan might influence the crystal density and the CaOx content. It can be concluded that glucomannan can potentially induce CaOx synthesis and crystal count increase. Keywords: Caox, Crystal, Development, Glucomannan, Interact, Porang.
Chemical Characterization of Silver Nanoparticle Compounds using Red Algae (Fucus vesiculosus) in Freeze Dry Methods Savitri, Evika Sandi; Shinta; Eko Budi Minarno; Rahmi Annisa
The Journal of Experimental Life Science Vol. 13 No. 1 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.01.08

Abstract

The nanoparticle green synthesis method is an alternative method for synthesizing nanoparticles or nanomaterials. The nanoparticle green synthesis method is a synthesis method that forms metal nanoparticles using natural materials such as plants as bioreductor. Red algae contain active ingredients that are the potential as antioxidants and anti-aging. Fucus vesiculosus is one of the red algae species with bioactive as an anti-aging and antioxidant compound. This study aims to synthesize, characterize and determine the antioxidant activity of F. Vesiculosus nanoparticles compounds. Characterization for nanoparticle compounds is Scanning Electron Microscope (SEM), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), and Particle Size Analyzer (PSA). The antioxidant activity test used the DPPH method. The results of the SEM characterization of nanoparticles showed the results of the formation of spherical particle surfaces known as a spherical formation. SEM-EDX shows several constituent elements of silver nanoparticles F. vesiculosus, namely C, O, Na, Mg, Cl, and K. Characterization silver nanoparticles using PSA measuring the particle size are 74.06 nm at 99.5% distribution. Characterization of silver nanoparticles using XRD showed crystalline character. The FTIR pattern shows the functional groups of alkenes, aromatic rings, ethers, amines/amides, alkenes, and alkanes. The antioxidant test results showed that the inhibition concentration for ascorbic acid was 1.13 ppm, silver nanoparticles F. vesiculosus 177.6 ppm, F. vesiculosus crude extract 1335 ppm, and respectively, ascorbic acid > silver NP > crude extract F. vesiculosus. The test results show that the antioxidant activity of the nanoparticle compounds increased from the very weak into the strong category.  Keywords: antioxidant activity, Fucus vesiculosus, Nanoparticle.

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