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The Journal of Experimental Life Sciences (JELS)
Published by Universitas Brawijaya
ISSN : 20872852     EISSN : 23381655     DOI : 10.21776/ub.jels
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 299 Documents
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Phaleria macrocarpa Leaves Extract Reduce Tumors Growth and Improve Histological Changes of Liver and Kidney on 4T1 Breast Cancer Mice Model Aminullah, Lela -; Christina, Yuyun Ika; Rifa'i, Muhaimin -; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 12 No. 2 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.02.02

Abstract

Breast cancer is a type of cancer that highly occurs globally and causes death cases. Of the many ways of treating breast cancer, chemotherapy is the most recommended, even though it causes various disturbing side effects. Therefore, alternative medicine using bioactive compounds of medicinal plants has begun to be widely used, for example, Phaleria macrocarpa, a plant native to Indonesia with anticancer and antioxidant activity. The liver and kidneys are important organs that function to maintain body homeostasis. The use of crude extracts of medicinal plants often causes damage to those organs at inappropriate doses. This research aimed to get an effective dose for reducing breast cancer growth and is safe for the liver and kidneys. A total of 36 mice were divided into six groups, including healthy control, cancer control, cisplatin, and three doses of P. macrocarpa extract (58.9, 117.8, and 235.6 mg.kg-1). The experimental animals were injected using a 4T1 cell line and treated orally using P. macrocarpa leaf ethanol extract for two and three weeks. The tumor volume of mice was measured periodically. At the end of treatment, mice were sacrificed, and their liver and kidney organs were isolated. Both organs were then prepared for H&E staining and observed using a microscope. The results showed that a dose of 58.9 mg.kg-1 and 117.8 mg.kg-1 of P. macrocarpa extract could reduce tumor volume by more than 90%, and the 117.8 mg.kg-1 dose is the safest dose to use because it does not affect the kidney and cause chronic damage to liver tissue. Keywords: extract, kidney, liver, tissue damage, tumor.
Analysis of Microplastics in Water and Biofilm Matrices in Metro River, East Java, Indonesia Sari, Safitri Permata; Kartikaningsih, Hartati; Yanuar, Adi Tiya; Kurniawan, Andi
The Journal of Experimental Life Science Vol. 12 No. 1 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.01.04

Abstract

The Metro River flows from upstream to downstream across East Java to support several human activities such as household, toilets, or agriculture. The utilization of water rivers must be balanced with water quality monitoring so that the quality of the water can be monitored. This study aims to analyze the abundance of microplastic in the Metro River. Moreover, the water parameters (pH, dissolved oxygen, temperature, flow velocity) were also measured. Sampling was carried out at three different stations, namely station 1 representing the agricultural activity area, station 2 representing the household activity area, and station 3 representing industrial activity. This study shows the abundance of microplastics in biofilm matrices and surrounding river water. The types of the microplastics are fibers, fragments, and films. The total abundances of microplastics in river water ranged from 0.8 - 1.61 particle.mL-1, while the biofilm matrices ranged from 7.4 to 9.5 particle.gram-1. The results of water quality parameters at all stations are still relatively good compared to quality standards. To the best of our knowledge, this study was the first study that reports the microplastics in the water of and inside biofilm formed on Metro River.
Analysis of Microplastics in Water and Biofilm Matrices in Lahor Reservoirs, East Java, Indonesia Pertiwi, Putri Rahayu; Mahmudi, Mohammad; Pramudia, Zulkisam; Kurniawan, Andi
The Journal of Experimental Life Science Vol. 12 No. 2 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.02.03

Abstract

One of the aquatic ecosystems that are very susceptible to contamination is the reservoir ecosystem. Reservoirs have different characteristics from other water bodies because they receive continuous water input from the river that flows through them. The river water contains organic and inorganic materials that can cause pollution caused by various kinds of human activities. The pollutant that is currently getting more attention is microplastics. Hence, various ways are developed to monitor the presence of microplastics in environments. The biotic component that may adsorb and accumulate microplastics is microbes that formed biofilm matrices as a predominant habitat. This study analyzes the presence of microplastics in water and biofilm matrices in Lahor Reservoir. The water parameters (pH, dissolved oxygen, temperature, and flow velocity) were also measured. Samplings were carried out at three stations, namely station A (inlet channel), station B (middle), and station C (outlet channel). This study revealed that water quality parameters were still relatively good according to the environment's quality standards. The average value of microplastic abundance in water at station A was 0.0013 particles.mL-1, station B was 0.00083 particles.mL-1, and station C was 0.00072 particles.mL-1. The average abundance of microplastics in the biofilm at station A, station B, and station C was 7.55 particles.g-1, 7.26 particles.g-1, and 4.59 particles.gram-1, respectively. This study indicates that the abundance of microplastics in the biofilm in the Lahor Reservoir was thousands of times higher than in the water. According to the results of this study, the biofilm can be used as a biological agent in monitoring the presence of microplastics in aquatic ecosystems such as the Lahor Reservoir, East Java, Indonesia. Keywords: Aquatic Ecosystem, Biofilm, Microbial Ecology, Microplastics, Water Pollutant.
Relationship between Metallothionein and Mercury (Hg) in the Gill Tissue of the Barbonymus altus in the Brantas River Jombang, East Java Aang Setyawan Anjasmara; Hertika, Asus Maizar Suryanto; Yanuhar, Uun
The Journal of Experimental Life Science Vol. 13 No. 1 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.01.09

Abstract

Pollution of river waters is most often the result of human activities in managing agricultural land, industry, and transportation. The entry of pollutants such as heavy metal mercury (Hg) into the waters can interfere with the survival of fish waters. Red Bader Fish (Barbonymus altus) can be used as a biomarker in analyzing aquatic environmental conditions in the Brantas River, Jombang, East Java. This study aimed to analyze the relationship between metallothionein and heavy metal mercury in the gill tissue of Barbonymus altus. Sampling was conducted at three stations with three replications from February to March 2022. Data analysis used regression and correlation methods to determine the relationship between metallothionein and metal mercury in gill tissue. The density and intensity of metallothionein in the gill tissue of Barbonymus altus were analyzed using the immunohistochemical method. The mercury (Hg) content in the gill tissue of Barbonymus altus was analyzed using Atomic Absorption Spectroscopy (AAS). The results showed that the metallothionein density at each station included station 1 of 252.52 × 10-6 – 497.79 × 10-6 MT.µm-2, station 2 of 277.78 × 10-6 – 378.78 × 10-6 MT.µm-2, and station 3 of 303.03 × 10-6 – 404.04 × 10-6 MT.µm-2. The relationship between the metallothionein density value and mercury in the gill tissue has a moderate correlation. In contrast, the metallothionein intensity value with mercury in the gill tissue strongly correlated with a significance value of sig < 0.05. The Hg levels in the gill tissue, density, and intensity have significant values at the all three locations based on the real difference test (sig. 0.00).   Keywords: Barbonymus altus, density, intensity, mercury, river.
Relationship of Circulating Endothelial Cells, Endothelial Progenitor Cells, and Endothelial Dysfunction after High-Intensity Exercise: Role of Nitric Oxide Kinanti, Rias Gesang; Sargowo, Djanggan; Widjajanto, Edi; Rifa’i, Muhaimin
The Journal of Experimental Life Science Vol. 13 No. 2 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.02.01

Abstract

Endothelial dysfunction after high-intensity exercise occurs through various mechanisms characterized by decreased nitric oxide (NO) bioavailability. This study aims to determine the relationship between circulating endothelial cells (CEC), endothelial progenitor cells (EPC), and endothelial dysfunction after high-intensity exercise through an experiment using male Wistar strain rats. This study uses the randomized control group posttest-only design. A total of 32 male Wistar rats aged 20-22 weeks with a body weight of 140±20 g were taken randomly and divided into two groups. Group 1 (n=16), the control group, was given low-intensity exercise. Meanwhile, group 2 (n=16), named the treatment group, was given high-intensity exercise. The groups were run on a special treadmill where light-intensity exercise is done at a speed of 5-8 meters.minute-1 for 30 minutes and high intensity at 25-30 meters.minute-1 for 30 minutes. The treatment was performed at 5 to 10 pm, 3 times weekly for 12 weeks. Blood and aortic tissue samples were taken after the mice were rested for 12 hours after the last exercise. ELISA and Flow cytometry measured NO, CEC, and EPC levels. This research showed that CEC and EPC expression and high-intensity exercise have a positive relationship, yet not significant to the occurrence of endothelial dysfunction (NO), with a value of Sig. (0.111); p > 0.05, and the relationship value is r = 0.414 (R²= 17.15%). In conclusion, endothelial dysfunction after high-intensity exercise is not associated with CEC and EPC expression, which indicates endothelial damage.  Keywords: exercise, circulating endothelial cells, endothelial dysfunction, endothelial progenitor cells, nitric oxide
The Effect of Alpinia Purpurata K. Schum Rhizome Extract Supplementation in Tris-Egg Yolk Extender on the Quality of Cryopreserved Bali Bull Sperm Firlina Laila Putri; Sri Rahayu; Agung Pramana Warih Marhendra
The Journal of Experimental Life Science Vol. 13 No. 2 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.02.03

Abstract

This study aimed to analyze the effect of Alpinia purpurata K. Schum rhizome extract (APRE) supplementation into tris-egg yolk extender on the sperm quality of Bali bull after freezing. This study used experimental method with a completely randomized design (CRD), consisting of five treatments, denoted as P0 (semen diluted using 100% Tris-Egg Yolk (TEY)), P1 (semen diluted using 98% TEY and 2% APRE), P2 (semen diluted using 96% TEY extender + 4% APRE), P3 (semen diluted using 94% TEY + 6% APRE), and P4 (semen diluted using 92% TEY + 8% APRE). Semen quality was assessed both before and after freezing, using several indicators such as motility, viability, and abnormality of sperm. The antioxidant activity of RGE was determined using the DPPH (1,1-diphenils-2-picrylhydrazils) technique. The data were analyzed using ANOVA with further analysis using post hoc tests in the SPSS 25 program. The results showed that APRE has strong antioxidant activity, with an IC50 value 38.004 μg.mL-1. Meanwhile, this research has shown that the addition of 6% RGE in the tris-Tris-egg yolk extender was able to maintain sperm quality before and after freezing. Keywords: Alpinia purpurata K. Schum, sperm quality, tris-egg yolk.
Piper betle L. Leaves Extract Potentially Reduce the Nitric Oxide Production on LPS-Induced RAW 264.7 Cell Lines Dwijayanti, Dinia Rizqi; Puspitarini, Sapti; Widodo, Nashi
The Journal of Experimental Life Science Vol. 13 No. 2 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.02.02

Abstract

Chronic inflammation can lead to several diseases that represent the leading causes of mortality worldwide. Conventional treatment of inflammation can carry some risks. Therefore, research on herbal medicine that are suspected of having anti-inflammatory effects, such as Piper betle L., is important. This study aims to investigate the effect of P. betle L. extract on nitric oxide as a pro-inflammatory mediator. The dried leaves of P. betle L. were extracted by ethanol. RAW 264.7 cells were treated with LPS and P. betle L. extract (PBE). Nitric oxide was measured by the Griess method. Antioxidant activity was determined using 2,2–Diphenyl-1-picrylhydrazyl (DPPH) method. Total flavonoids and phenolic content were also identified by aluminium chloride and Folin–Ciocalteu colorimetric assay, respectively. This study demonstrated that the PBE has excellent NO suppression activity with the IC50 56.22±16.41 μg.mL-1, without cytotoxicity. PBE also has DPPH inhibitory concentration IC50 values of 279.67±11.36 ppm. Interestingly, PBE has a flavonoid content of 50.17±3.14 mg QE.g-1 and phenolic content of 128.92±1.2 mg GAE.g-1. These compounds are thought to be responsible for its anti-inflammatory as well as antioxidant. This study proved that P. betle L. leaves extract could be used as a candidate for anti-inflammatory drugs. Nevertheless, further research about the biological activity mechanism and their bioactive compounds' purification is still required. Keywords: Inflammation, lipopolysaccharide, Macrophage, Nitric Oxide, Piper betle. 
Synergism of Lecanicillium lecanii (Zimm) and Chromolaena odorata L. Leaf Extract to Control Aphis gossypii (Glover) in Chili Plants Nurhayati, Wiwik; Haryadi, Nanang Tri
The Journal of Experimental Life Science Vol. 12 No. 1 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.01.05

Abstract

The purpose of the study was to determine the effect of the appropriate and effective application of the L. lecanii fungus and C. odorata L. leaf extract in increasing the mortality of A. gossypii. The study was divided into two stages. The first stage was in vitro test of L. lecanii synergism plus C. odorata L. leaf extract in PDA media with four treatments, namely LK0= L. lecanii 107 conidia.mL-1 plus 0% C. odorata L. leaf extract, LK1= L. lecanii 107 conidia.mL-1 plus 10% C. odorata L. leaf extract, LK2= L. lecanii 107 conidia.mL-1 plus 25% C. odorata L. leaf extract; LK3= L. lecanii 107 conidia.mL-1 plus 40% C. odorata L. leaf extract. The second stage was the toxicity test of the application of L. lecanii suspension and C. odorata L. leaf extract on mortality of A. gossypii. The toxicity test was based on the results of the synergism test, where the addition of C. odorata L. leaf extract to L. lecanii growing media showed incompatible results. Therefore, the toxicity test was carried out separately with five treatments, namely H0= Control (aqua dest); H1= Conidia suspension L. lecanii 107 conidia mL-1; H2= 10% C. odorata leaf extract; H3= 25% leaf extract of C. odorata; H4= 40% C. odorata leaf extract. The results showed that the compatibility test of L. lecanii with leaf extract of C. odorata L. was incompatible and classified as toxic. The addition of C. odorata L. leaf extract in concentrations of 10%, 25%, and 40% could significantly inhibit colony growth, sporulation, and conidia viability of L. lecanii, with a higher level of inhibition as the concentration of C. odorata L. leaf extract, was added. The toxicity test of a separate application of C. odorata L. leaf extract and L. lecanii suspension had a significant effect on mortality of 3rd instar nymph A. gossypii, with the highest mean mortality found in a single application of 40% C. odorata L. leaf extract with an average mortality of 100% at 96 HAA(Hours After Application) observations. Keywords: A. gossypii, C. odorata L.  leaf extract, L. lecanii, synergism.
Expression of IL-17 on Breast Cancer Mice Treated by Combination of Phyllanthus Urinaria and Catharanthus roseus Extract Shofiyah, Aya; M. Sasmito Djati; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 12 No. 2 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.02.05

Abstract

This study aimed to evaluate the effect of combination Phyllanthus urinaria and Catharanthus roseus in breast cancer mice based on the expression of IL-17. 7,12-Dimethylbenz(α)anthracene (DMBA) was injected intraperitoneally into normal mice at dose 1.5 mg.kg-1 weight to obtain breast cancer mice. Total of 24 experimental mice divided into normal mice (N), breast cancer mice (K), breast cancer mice with cisplatin (C) treatment (5 mg.kg-1 weight), breast cancer mice with combination extract Dose 1 (P. urinaria 500 mg.kg-1 weigt + C. roseus 15mg.kg-1 weight), breast cancer mice with combination extract Dose 2 (P. urinaria 1000 mg.kg-1 weight + C. roseus 75mg.kg-1 weight), and breast cancer with combination extract Dose 3 (P. urinaria 2000 mg.kg-1 weight + C. roseus 375 mg.kg-1 weight). Cheral was given orally for 14 days. The level of IL-17 was evaluated by flow cytometry analysis. The combination can suppress the expression of IL-17 which down regulation of IL-17 indicate a good prognosis for the breast cancer mice, for 6.17% in breast cancer condition to 0.93% with Dose 3 treatment. The combination can be used as immunomodulatory agent in humoral immunity through the regulation of IL-17. Keywords: Breast cancer, Catharanthus roseus, IL-17, Phyllanthus urinaria
Effect of Active Detergent Ingredients on Successful Fertilization and Embryo Development of Sea urchins Tripneustes gratilla (Linnaeus, 1758) Molle, Aprilia Paskarani; Marhendra, Agung Pramana Warih; Sri Rahayu
The Journal of Experimental Life Science Vol. 12 No. 2 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.02.04

Abstract

The success of fertilization and development of sea urchin embryos Tripneustes gratilla can be used as a bioindicator of the water quality against the accumulation of pollutants. One of the contaminants that are often used is detergent with an active ingredient in the form of LAS. The purpose of this study was to analyze the effect of LAS on the success of fertilization and development of sea urchin embryos. This research was conducted in the Zoology Laboratory, Pattimura University, Ambon. Tripneustes gratilla were treated with exposure to the active ingredient LAS with a concentration of 0.0; 0.5; 1, and 2 mg.L-1. The parameter used to assess the success of fertilization is the formation of membrane fertilization. Parameters of embryo development are the division of 2, 4 , 8 , 16 , and 32 cells to form a morula, blastula, and hatching blastula. The results showed that the active ingredient of detergent LAS with a concentration of 0.5 mg.L-1 caused delays in the process of fertilization and embryo development. Meanwhile, the active ingredient LAS with concentrations of 1 and 2 mg.L-1 caused failure in the fertilization process and the embryonic development process of T. gratilla. Keywords: Detergent, embryo, fertilization, Tripneustes gratilla.

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