cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Jehan Ramdani Hariyati
Contact Email
jehanramdani@ub.ac.id
Phone
+6282333752235
Journal Mail Official
jehanramdani@ub.ac.id
Editorial Address
Jl. Veteran Malang 65145
Location
Kota malang,
Jawa timur
INDONESIA
The Journal of Experimental Life Sciences (JELS)
Published by Universitas Brawijaya
ISSN : 20872852     EISSN : 23381655     DOI : 10.21776/ub.jels
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 299 Documents
 25   26   27   28   29 
Influence of Marsilea crenata and Alpinia purpurata Ethanol Extract on MDA and SOD Testicular Cells of Hyperglycemia Mice Damayanti, Febriane Eka; Rahayu, Sri; Widyarti, Sri
The Journal of Experimental Life Science Vol. 13 No. 3 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.03.04

Abstract

Hyperglycemia can induce testicular damage that leads to male infertility. Herbal plants, such as Marsilea crenata and Alpinia purpurata, are used for antioxidant defense systems to repair reproductive disorders due to hyperglycemia. This study aims to evaluate the effect of M. crenata and A. purpurata ethanol extracts on MDA and SOD testes of hyperglycemia mice. This study used a completely randomized design with seven treatment groups (n=4), namely N (control group), H (hyperglycemia mice), Met (hyperglycemia mice given metformin), D1 (0. 09 mg.g-1 M. crenata), D2 (0.2 mg.g-1 A. purpurata), D3 (0.09 mg.g-1 M. crenata + 0.2 mg.g-1 A. purpurata), and D4 (0.09 mg.g-1 M. crenata + 0.4 mg.g-1 A. purpurata). The extracts were administered orally for 17 days. Analysis of testicular MDA and SOD levels was performed by flow cytometry. Data analysis was performed with a one-way ANOVA test and continued with the Tukey test. The results showed that the D4 treatment group, compared to D3, D2, D1, Met, H, and N, showed a better decrease in MDA levels (4.47%) and an increase in SOD levels in the D4 group (4.77%). The research concludes that the combination of 0.09 mg.g-1 M. crenata and 0.4 mg.g-1 A. purpurata was an optimal dose to decrease MDA levels and increase SOD levels in the testes of hyperglycemic mice. Keywords: Alpinia purpurata, Marsilea crenata, MDA, SOD, testes.
Phylogenetic Inference on Limnonectes kuhlii Complex in Java and Sumatra Reveals Significant Novel Diversity Fahmi, Muhamad; Kadafi, Ahmad Muammar; Priambodo, Bagus; Fauzi, Muhammad Alif; Hamidy, Amir; Firdaus, Anggun Sausan; Smith, Eric Nelson; Kurniawan, Nia
The Journal of Experimental Life Science Vol. 13 No. 3 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.03.02

Abstract

The fanged frog, Limnonectes kuhlii, exemplifies the complexity of cryptic species. Though originally described from Java, subsequent studies indicate that L. kuhlii encompasses multiple lineages, suggesting the presence of several undescribed species. Suspecting the existence of multiple undescribed species within the L. kuhlii complex in Sumatra, we collected 17 specimens from Java and Sumatra. We extracted the 12S, tRNAval, and 16S mitochondrial DNA from these specimens for phylogenetic analysis using Maximum Likelihood and Bayesian Inference methods and for estimating time divergence. Our findings uncovered two novel diversity within the L. kuhlii complex both in Sumatra and Java. Furthermore, our estimation of time divergence suggests that the diversification of L. kuhlii in Java and Sumatra was influenced by geological and climatic events, including landmass emergence and sea-level fluctuations. In conclusion, our research provides critical insights into the evolutionary complexity and diversity of the L. kuhlii complex in Sumatra and Java. While our molecular evidence suggests novel diversity, further comprehensive morphological studies are imperative to confirm and describe these potential new species. Furthermore, additional sampling across its range and deeper investigations integrating both molecular and morphological data are crucial. Keywords: fanged frogs, Limnonectes kuhlii, Limnonectes sisikdagu, phylogenetics.
Effect of Stirring Speed and Solid to Solvent Ratio on Fucoidan Yield from Sargassum sp. Hasah, Naili Uswatun; Wati, Dewi Purnama; Sugiono, Sugiono
The Journal of Experimental Life Science Vol. 13 No. 3 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.03.05

Abstract

Brown alga Sargassum sp. has bioactive alginate and fucoidan, which have the potential for raw materials in the biorefinery industry with core processing of fucoidan and alginate extraction. Fucoidan is a polysaccharide found in the cell wall matrix of brown alga Sargassum sp. and functions as an anti-cancer, immunomodulatory, and anti-inflammatory. This research aims to determine the effect of stirring speed and solid-to-solvent ratio on fucoidan yield. A 2K factorial experimental design was used to determine the effect of process conditions, stirring speed, and solid-to-solvent ratio on fucoidan yield. Analysis of yield response data and model accuracy was carried out by regression analysis using the Design Expert program. The results showed that the stirring speed and solid-to-solvent ratio affected the yield of fucoidan. The highest fucoidan yield of 3.77% occurred at a stirring speed of 750 rpm and a solid-to-solvent ratio of 1:20 (g.mL-1). The first-order experimental model is quadratic. The model test shows that the curvature is significant at the confidence level α = 0.05. Keywords: Fucoidan, Sargassum sp, stirring speed, solid to solvent ratio.
Effectiveness of Extract Supplements (Apium graveolens L.) to Estrogen Receptors Expression and Oocyte Diameter at Nucleolar Chromatin and Perinucleolar Stages in Female of Nile tilapia (Oreochromis niloticus L.) Rikardo, Novy Kurnia; Marhendra, Agung Pramana Warih; Kurniawan, Nia; Riawan, Wibi; Rahayu, Sri
The Journal of Experimental Life Science Vol. 13 No. 3 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.03.03

Abstract

The study of the use of celery (Apium graveolens L.) in the health sector has been widely discussed. The analysis of the use of celery in the process of oocyte development still needs to be addressed. This study was conducted to see the effect of celery extract on the expression of estrogen receptors on the gonads and oocyte diameter in tilapia for the first time. Fish are divided into four groups: P0 = Control treatment, feed without mixture; P1 = Treatment with celery extract concentration of 2,000 mg.kg-1 of feed; P2 = Treatment with celery extract concentration of 4,000 mg.kg-1 of feed; P3 = Treatment with celery extract concentration of 6,000 mg.kg-1 of feed. The results showed celery extracts expressed estrogen receptors on the gonads. The expressed estrogen receptor is the estrogen receptor β, whereas the estrogen receptor α is not detected in the gonad. Group statistical tests ANOVA showed no significant results in the development of oocytes. On the other hand, celery extract also impacted the development of oocytes at the nucleolar stage of chromatin, with a markedly greater oocyte diameter. Meanwhile, in the perinucleolar stage, celery extract reduces the oocyte diameter. Keywords: Apium graveolens L, ERα, ERβ, nucleolar chromatin, perinucleolar.
Effect of Combination between the Extract of Marsilea crenata and Alpinia purpurata K.Schum Rhizome on Sperm Quality of Hyperglycemia Mice Shofi Nur Aliyah; Sri Rahayu; Agung Pramana Warih Marhendra
The Journal of Experimental Life Science Vol. 13 No. 3 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.03.06

Abstract

This study aims to analyze the effect of the combination between water clover (Marsilea crenata) and red galangal rhizome (Alpinia purpurata) on sperm quality of hyperglycemia model mice. Mus musculus were randomly divided into eight groups: non-hyperglycemia (P0), hyperglycemia (P1), metformin 0.375 mg.kg-1 (P2), hyperglycemia with M. crenata extract dose 0.09 mg.g-1 (P3), A. purpurata dose 0.20 mg.g-1 (P4), combination of M. crenata (0.09 mg.g-1) - A. purpurata (0.20 mg.g-1) (P5), combination of M. crenata (0.09 mg.g-1) - A. purpurata (0.40 mg.g-1) (P6), and combination of M. crenata (0.18 mg.g-1) - A. purpurata (0.20 mg.g-1) (P7). Hyperglycemia mice were induced by streptozotocin 145 mg.kg-1. STZ induction and oral extract addition were carried out for 20 days. Sperm quality parameters observed in this study included motility, viability, and concentration. Sperm quality data were statistically analyzed with One-Way ANOVA followed by the Tukey HSD test using the SPSS 23 for Windows program (p<0.05). This research demonstrates that adding M. crenata, A. purpurata, and their combination for 20 days can significantly increase motility, viability, and sperm concentration in hyperglycemia-model mice. The combination of M. crenata and A. purpurata in the ratio of 1:1 was considered effective in improving the sperm quality of hyperglycemia model mice. Keywords: A. purpurata, hyperglycemia, M. crenata, oxidative stress, sperm quality.
Reduction of Remazol Brilliant Blue-R Dye Levels Through Electrocoagulation Process at Laboratory Scale to Reduce Pollution Levels of Textile Industrial Waste in River Streams Sholichin, Moh.; Ismuyanto, Bambang; Saptati N. H., A. S. Dwi; Susilo, H.
The Journal of Experimental Life Science Vol. 13 No. 3 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.03.07

Abstract

The textile industry produces liquid waste, which is the main cause of pollution in rivers. Textile industry wastewater has a dye content of about 40%. Electrocoagulation is one method that is able to reduce waste by using electrolysis reactions. This study aims to analyze the level of the electrode plan’s capability to reduce the level of dye waste pollution from the industry on a laboratory scale. The submerged electrode material was 7 cm x 8 cm, 7 cm x 10 cm, and 7 cm x 12 cm in dimensions. The electrocoagulation process was run for 120 minutes with a voltage of 30 volts and stirring at 200 rpm. Samples were taken at 0, 5, 15, 30, 45, 60, and 120 minutes for the electrocoagulation process. Determination of the maximum wavelength of Remazol Brilliant Blue-R was carried out by measuring 60 ppm of Remazol Brilliant Blue solution at various wavelengths in the range of 400 - 800 nm with 1 nm intervals. Measurements were carried out using a UV-Vis spectrophotometer. First-order and second-order reaction kinetics models were used to study the reaction kinetics of the Remazol Brilliant Blue-R electrocoagulation process. The highest efficiency in the Remazol Brilliant Blue-R reduction process was obtained at 86% at 120 minutes. The kinetics of the Remazol Brilliant Blue-R electrocoagulation process using aluminum-based electrodes was under the first order with an R2 value > 0.96. Keywords: Electrocoagulation, Industrial Wastewater, Remazol Brilliant Blue-R, UV-Vis Spectrophotometer.
Preventive Effect of Antioxidant From Purple Rosella Yoghurt on Duodenum and Colon Histopathology of Rattus norvegicus Exposed to Rhodamine B And Saccharin Haskito, Ajeng Erika Prihastuti; Nariswari, Bintari; Safitri, Anna; Noviatri, Aldila; Untari, Handayu; Sari, Citra
The Journal of Experimental Life Science Vol. 14 No. 1 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.01.04

Abstract

Rodhamine B and Saccharin are often misused by food producers as food additive, which, if their use is not monitored, can produce free radicals that cause oxidative stress conditions in the body, leading to dangerous damage to the body. The addition of purple rosella in yoghurt can increase the functional value of the yoghurt, namely increasing the antioxidant content. The purpose of this study was to determine the benefits of giving purple rosella yoghurt in preventing damage to the duodenum and colon of (Rattus norvegicus) caused by Rodhamine B and Saccharin. This study used Completely Randomized Design (CRD) with 28 male R. novergicus, Wistar strain in aged 8-12 weeks. Group K was not given any treatment; R was given Rodhamine B, S was given Saccharin, KRS was given Rodhamine B and Saccharin, YR was given Rodhamine B and yoghurt, YS was given Saccharin and yogurt, YRS was given Rodhamine B, Saccharin and yogurt. The dose of Rodhamine B was 22.5 mg.kg-1, Saccharin was 157.77 mg.kg-1, and the volume of yoghurt was 1 mL.head-1. They were administered orally with a gastric tube for 14 days. The concentration of purple rosella extract added to yoghurt was 15% (v/v). Parameters were observed in histopathology of the duodenum and colon with the scoring method. Data analysis was done with the Kruskal-Wallis test followed by the Mann-Whitney test. The KRS group showed the heaviest damage, namely necrosis, villi erosion, and inflammatory cell infiltration of >75% of the total visual field in the duodenum and colon. The YR, YS, and YRS groups showed significant changes compared to the R, S, and KRS groups, namely necrosis, villous erosion, and inflammatory cell infiltration, which appeared to be 25-50% of the total visual field. However, this did not match the condition of group K, which appeared to be <25% of the total visual field. The provision of purple rosella yoghurt can prevent 50% damage to the duodenum and colon of Rattus novergicus due to Rodhamine B and Saccharin. Keywords: colon, duodenum, Rodhamine B, Saccharin, yoghurt.
Potency of Coconut Shell Biochar to Remediate Ion Chromium in Contaminated Water Faris, Salman; Titah, Harmin Sulistiyaning; Pratikno, Herman
The Journal of Experimental Life Science Vol. 13 No. 3 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.03.08

Abstract

Many heavy metals contaminate our environment, including chromium (Cr(VI) contaminated water. The aim of this study was to determine the removal of ion Cr(VI) using the coconut shell biochar. Biochar activation processes were conducted at 350℃ and 550℃. The characteristic tests of biochar were conducted, such as testing for moisture, ash, volatile, and carbon content. Additionally, a scanning electron microscope was also performed. The remediation test was carried out on a laboratory scale with a batch system. The variables were the mass of coconut shell biochar (0, 8, and 16 r) and the time of process were 0, 30, and 60 min. Based on the results, the characteristics of coconut shell biochar showed a high carbon content, which reaches 80.4% for activation temperature at 500℃ and 73.02% for 350℃. Coconut shell biochar can reduce ion Cr(VI) in water; it reached 73.78% to 88.15%. The percentage of Cr(VI) reduction in water reached 88.15% using biochar that was activated at a temperature of 350℃ and a mass of 16 g at a detention time of 60 min. In conclusion, coconut shell biochar has good potential to reduce chromium in water. Keywords: Biochar, Chromium, Coconut Shells, Remediation.
Histological and Biochemical Evaluation of the Protective Potential of Ascorbate and Alpha-Tocopherol against Cypermethrin-Induced Toxicity Adeniyi, Temidayo; Moronkeji, Akinpelu; Fikayomi, Akinrimisi
The Journal of Experimental Life Science Vol. 14 No. 1 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.01.05

Abstract

The unrestricted and unsystematic use of cypermethrin pesticides has detrimental effects on the organs, ranging from short-term sickness to long-term effects. The ameliorating effect of alpha-tocopherol and ascorbate was investigated singly and in combination with cypermethrin-induced oxidative stress using murine models. Additionally, the livers and kidneys of rats were histologically evaluated. Twenty-five (25) adult male Wistar rats with an average weight of 190 g were allocated randomly into five groups consisting of five rats each. Group I consists of the unexposed control rats, while rats in groups II-V were the test group exposed to cypermethrin at standard doses of 10 mg.kg-1 bw. While rats in group II were exposed and untreated, group III-V was administered with ascorbate (5000 mg.kg-1 bw), alpha-tocopherol (3000 mg.kg-1 bw), and co-administered with both vitamins at their standard doses, respectively. Regimen administration was by gavage for 28 days, and while the vitamins were administered daily, cypermethrin exposure was done twice a week. At the end of the experiment, rats were euthanized, and blood obtained via cardiac puncture was used for biochemical analysis, while the liver and kidneys excised were processed for histopathological evaluation. Results revealed elevated aspartate transferase (AST), alanine transferase (ALT), malondialdehyde (MDA), and creatinine levels. At the same time, a decrease in superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activities was observed in the test group (p<0.05). Additionally, treatment with ascorbate and alpha-tocopherol co-administration reversed the biochemical parameters in the exposed rats. Conclusively, ascorbate and alpha-tocopherol ameliorate oxidative damage associated with cypermethrin exposure. Keywords: Cypermethrin, Histopathology, Pesticides, Toxicity, Vitamins.
Elephantopus scaber L. Ethanolic Leaves Extract Modulates IL-2 Production and T-Lymphocyte Activation in Pulmonary Fibrosis Mice Model Izzah, Fathiyah Nurul; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Rifa'i, Muhaimin; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 14 No. 1 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.01.01

Abstract

Pulmonary fibrosis is a chronic disease characterized by progressive connective tissue deposition that replaces healthy lung tissue. This study aimed to investigate the effect of Elephantopus scaber L. Ethanol Extract (ESEE) treatment on the relative number of IL-2 cytokine and lymphocyte activation in bleomycin (BLM)-induced pulmonary fibrosis mice model. Fifty-six male BALB/c mice were divided into seven treatment groups: N (normal); V or vehicle (corn oil); PF or Pulmonary Fibrosis (BLM 2 mg.kg-1); Dex (Dexamethasone 3 mg.kg-1 + BLM); D1-D3 (ESEE at doses of 0.0504, 0.1008, and 0.2016 mg.kg-1 BW + BLM). ESEE, dexamethasone, and corn oil were administered orally, followed by intraperitoneal bleomycin injection daily for 14 days. Mice were dissected on days 7 and 14, and spleens were isolated to analyze cell populations expressing CD4+IL-2+, CD8+IL-2+, CD4+CD62L+, and CD8+CD62L+. The results showed that bleomycin injection could increase the relative number of IL-2 and decrease the relative number of naive T cells compared to normal mice. ESEE treatment significantly reduced the relative number of IL-2, thus decreasing naive T cell activation after one week of bleomycin injection compared to the mice model. In contrast, the increased IL-2 production led to the increasing naive T cell activation after two weeks of bleomycin injection. Therefore, ESEE treatment has the potential to maintain homeostasis through modulation of IL-2 production and T-lymphocyte activation in the pulmonary fibrosis mice model. Keywords: Elephantopus scaber, IL-2, lymphocytes, mice, pulmonary fibrosis.

Page 27 of 30 | Total Record : 299

 25   26   27   28   29 

Filter by Year

2011 2025


Reset
Filter By Issues
All Issue Vol. 15 No. 3 (2025) Vol. 15 No. 2 (2025) Vol. 15 No. 1 (2025) Vol. 14 No. 3 (2024) Vol. 14 No. 2 (2024) Vol. 14 No. 1 (2024) Vol. 13 No. 3 (2023) Vol. 13 No. 2 (2023) Vol. 13 No. 1 (2023) Vol. 12 No. 3 (2022) Vol. 12 No. 2 (2022) Vol. 12 No. 1 (2022) Vol. 11 No. 3 (2021) Vol. 11 No. 2 (2021) Vol. 11 No. 1 (2021) Vol. 10 No. 3 (2020) Vol. 10 No. 2 (2020) Vol. 10 No. 1 (2020) Vol. 9 No. 3 (2019) Vol. 9 No. 2 (2019) Vol. 9 No. 1 (2019) Vol. 8 No. 3 (2018) Vol. 8 No. 2 (2018) Vol. 8 No. 1 (2018) Vol. 7 No. 2 (2017) Vol. 7 No. 1 (2017) Vol. 6 No. 2 (2016) Vol. 6 No. 1 (2016) Vol. 5 No. 2 (2015) Vol. 5 No. 1 (2015) Vol. 4 No. 2 (2014) Vol. 4 No. 1 (2014) Vol. 3 No. 2 (2013) Vol. 3 No. 1 (2013) Vol. 2 No. 2 (2012) Vol. 2 No. 1 (2012) Vol. 1 No. 2 (2011) Vol. 1 No. 1 (2011) More Issue