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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 299 Documents

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Kajian Penggunaan Ciprofloxacin terhadap Hematologi Ikan Botia (Botia macracanthus, Bleeker) yang Diinfeksi Bakteri Aeromonas hydrophila Maftuch Maftuch; Happy Nursyam; Sukarni Sukarni
The Journal of Experimental Life Science Vol. 2 No. 2 (2012)
Publisher : Postgraduate School, Universitas Brawijaya

Uji hematologi sangat bermanfaat sebagai alat diagnostik didalam menetapkan status kesehatan ikan. Salah satu aspek dari infeksi adalah terjadinya perubahan gambaran darah. Pemeriksaan darah dapat digunakan sebagai indikator keparahan suatu penyakit tertentu. Beberapa parameter yang dapat memperlihatkan perubahan patologi pada darah meliputi jumlah leukosit, deferensial leukosit, eritrosit. Dari hasil penelitian diketahui jumlah rata-rata sel darah merah dalam darah ikan botia sehat pada masing-masing perlakuan K-, K+, A, B, C, D berturut-turut adalah 2.470.000, 2.500.000, 2.560.000, 2.350.000, 2.440.000, dan 2.430.000. Sementara itu pada ikan botia yang terinfeksi bakteri A.hydrophila diketahui jumlah rata-rata eritrosit pada masing-masing perlakuan K-, K+, A, B, C, dan D berturut-turut adalah 2.420.0000, 1.770.000, 1.780.000, 1.890.000, 1.780.000, dan 1.870.000. jumlah rata-rata eritrosit ikan botia yang terinfeksi bakteri A.hydrophila, dan kemudian diobati dengan ciprofloxacin pada masing-masing perlakuan K-, K+,A, B, C, dan D berturut-turut adalah 2.420.000, 1.630.000, 2.090.000, 2.280.000, 2.500.000, dah 2.540.000. Sementara jumlah rata-rata sel darah putih dalam darah ikan botia yang sehat pada masing-masing perlakuan K-, K+, A, B, C, D berturut-turut adalah 97.000, 90.000, 88.000, 87.000, 93.000 dan 88.000. Sementara itu jumlah rata-rata sel darah putih pada ikan botia yang terinfeksi bakteri A.hydrophila, pada masing-masing perlakuan K-, K+, A, B, C, D berturut-turut adalah 97.000, 174.000, 174.000, 179.000, 185.000 dan 185.000. Sedangkan dari hasil penelitian terhadap ikan botia yang terinfeksi A.hydrophila dan kemudian diobati dengan ciprofloxacin diperoleh hasil perhitungan rata-rata leukosit pada masing-masing perlakuan berturut-turut adalah sebagai berikut 980.000, 189.000, 125.000, 137.000, 133.000, dan 136.000. Kata Kunci : Aeromonas hydrophila, eritrosit, hemotologi, ikan botia, leukosit

The Impact of Dissolved Nitrate and Phosphate on Maximum Growth Rate and Carrying Capacity of Oscillatoria in Intensive Shrimp (Litopenaeus vannamei) Farming Pond Situbondo, East Java, Indonesia Dian Aliviyanti; Suharjono Suharjono; Catur Retnaningdyah
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

The aims of study are to analyze the effect of dissolved nitrate and phosphate content of the intensive shrimp farming pond Situbondo to maximum growth rate and carrying capacity of Oscillatoria population density in the laboratory. This is an experimental research method using completely randomized design with three replications. The treatment were variation of nitrate and phosphate concentration (N0; N6; N12; N24; N48; P0,2; P0,4; P0.8, P1.6 mg.L-1). Experiment was done using a pure Oscillatoria culture in condition 25 watt lamp; 12 hours a day. The initial amount of Oscillatoria cells used for the treatment is 8 - 15 x 104 cell.mL-1. During the incubation process, chemical parameters were also observed including nitrate, phosphate, DO, and pH at the beginning of the incubation period. Oscillatoria cell was count every day until stationary phase for 30 days. Furthermore, a different test between treatments was conducted to determine levels of nitrate and phosphate in triggering the blooming of Oscillatoria using Oneway ANOVA analysis with SPSS Program. The results showed that the intensive shrimp pond waters of Situbondo already contain dissolved phosphate between 0.4 - 0.5 mg.L-1. Oscillatoria growth is strongly influenced by dissolved phosphate content in waters, phosphate levels of 0.2 - 0.4 or equivalent to the actual level of 0.6 - 0.7 mg.L-1 can cause the highest abundance of Oscillatoria that could endanger the ecosystem. Keywords: dissolved phosphate, intensive shrimp farming, Nitrate, Oscillatoria.

Potential of Dayak Onion (Eleutherine palmifolia (L) Merr) Extract As Antibacterial Against Pseudomonas fluorescens Immaria Fransira; Uun Yanuhar; Maftuch Maftuch
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Dayak onions (E. palmifolia (L) Merr) is a herb used as medicine and known to have antibacterial activity. This research aimed to evaluate antibacterial activity of Dayak onion (Eleutherine palmifolia (L) Merr) extract against fish pathogen Pseudomonas fluorescens. The method used in this study was extraction by maceration, UV-Vis spectrophotometry analysis on the extract and antibacterial activity test with Minimum Inhibitory Concentration (MIC) as well as disc diffusion test. The results of this study showed that the yield value was 8.87%. Based on UV-Vis analysis, it confirmed that extract of Dayak onion (Eleutherine palmifolia (L) Merr) contains predominantly flavonoids and phenols with its derivatives, that have antibacterial activity. The results of MIC and disc diffusion test showed potential antibacterial activity against P. fluorescens, as observed from the value of absorbance and inhibition zones that formed. Keywords: Antibacterial, Disc diffusion, Eleutherine palmifolia (L) Merr, MIC, Pseudomonas fluorescens.

Perkembangan Sel T Regulator Periferal dan Mekanisme Supresi in vitro Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 1 No. 1 (2011)
Publisher : Postgraduate School, Universitas Brawijaya

Sel T regulator yang ditandai dengan CD4+CD25+Foxp3 memegang peranan kunci pada sistem toleran tubuh. Sel T regulator ini diperlukan untuk mengendalikan sel efektor yang teraktivasi. Sel T regulator melakukan fungsinya sebagai pengendali sel efektor dan pembentuk sistem toleran dengan cara tidak hanya sebagai supresor namun juga pengatur sistem homeostasis. Sel T regulator mempunyai daya kendali terhadap sel lain yang terlibat pada sistem imun. Kemampuan mengendalikan sel lain ini mutlak diperlukan untuk menghindari terjadinya penyakit autoimun dan penolakan transplantasi. Kerja sel T regulator mempengaruhi respon imun terhadap alergen namun melemahkan sel efektor melawan sel tumor dan patogen. Di samping itu pada beberapa kasus diketahui bahwa sel T regulator juga menyebabkan sel efektor kehilangan fungsinya. Sampai sekarang mekanisme kerja sel T regulator secara seluler dan molekuler belum sepenuhnya diketahui, namun ada bukti tentang pentingnya sel ini dari banyak aspek biologi. Pengetahuan tentang adanya strategi mengaktifkan dan menginduksi munculnya sel T regulator dari sel T konvensional membuka kemungkinan untuk melakukan rerkayasa in vivo sebagai langkah imunoterapi khususnya pada model penyakit autoimun. Bahasan saat ini merangkum pengetahuan tentang sel T regulator sebagai imunoterapi dan harapan dimasa depan terhadap pemanfaatan sel ini.Kata kunci: autoimun, CD4+CD25+Foxp3+, sel efektor, Sel T regulator

Latent and Eggs Production of Banggai Cardinal (Pterapogon kauderni, Koumans 1933) on Various Salinity Levels: Conservation Efforts Atiek Pietoyo; Sri Andayani; Agoes Suprijanto
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

The aim of this study is to determine the best salinity level on latent and eggs production of Banggai Cardinal (Pterapogon kauderni Koumans 1993) based on first brood and brood production of Banggai Cardinal in the treatment. Water quality measurement was carried out every day to maintain water quality. Water quality measurement showed suitable salinity for Banggai Cardinal breeding. Brood latent showed no significant difference between the treatments. Total of 27 ppt is the level of salinity for Banggai Cardinal reproduction to gain positive respond on eggs production (42.3333 ± 7 eggs). Keywords: Banggai Cardinal, Eggs Production, Latent, Salinity.

Anti-hyperglycemic and Immunomodulatory Activity of a Polyherbal Composed of Sesbania grandiflora, Salacca zalacca and Acalypha indica Ahmad Zamroni; Elok Zubaidah; Muhaimin Rifa'i; Simon Bambang Widjanarko
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2018.008.03.09

Diabetes has become a serious global public health problem due to its high prevalence and mortality. Unfortunately, current anti-diabetic drugs are having some limitations and adverse effects. Therefore, searching for a new anti-diabetic agent is an urgent challenge. In this research, we examined the effectiveness of a traditional anti-diabetic polyherbal composed of Sesbania grandiflora seeds, Salacca zalacca leaves and Acalypha indica roots (2:1:1). The study was aimed to explore the anti-hyperglycemic effect of the polyherbal in STZ-induced diabetic mice and to investigate the immunomodulatory activity involved in the process of controlling hyperglycemia. Our results showed that the polyherbal water extract (150 mg.kgBW-1) could suppress blood glucose elevation and preserve pancreatic islet of diabetic mice. Moreover, the polyherbal treatment could normalize the relative amount of activated CD4+CD62L- and CD8+CD62L- T cells. The polyherbal extract also stimulated the production of CD4+CD25+FoxP3+ regulatory T cells (Tregs) which is known to play an important role in diabetes control. In addition, polyherbal treatment also increased the relative amount of anti-inflammatory cytokines IL-10 and TGF-β. These results revealed that the polyherbal extract has an anti-hyperglycemic and immunomodulatory activity that may provide beneficial function in diabetes healing. Keywords: Antidiabetes, Immunomodulator, Polyherbal, Regulatory T cell.

Bioactivity of Purple Yam Tuber (Dioscorea alata L.) on the Level of CD8+and CD8+CD462L+ T cells and Histology of Liver in BALB/c Mice Model of Digestive Allergy Yuyun Ika Christina; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 4 No. 2 (2014)
Publisher : Postgraduate School, Universitas Brawijaya

Purple yam tuber (Dioscorea alata L.) is a family of Dioscoreaceae containing diosgenin which is known as immunomodulatory agent. This study aimed to understand the quantitative changes of naïve and activated memory of T cells on mice model of digestive allergy after orally treated with ethanol extract of purple yam tuber. In this experiment, architecture of hepar histopathology is also observed. Ethanol extract of purple yam tuber with three doses of 0.167 g/kg bw (U1), 2.008 g/kg bw (U2), and 10.039 g/kg bw (U3) are applied. Data were analyzed using One-way ANOVA (p <0.05) and Tukey test using SPSS 16.0 for Windows. Ethanol extract of purple yam tuber triggers the immunocompetent activity of T cells in mice model of digestive allergy. The result showed that the number of memory type T cells in mice model of digestive allergy decreased in lower dose (0.167 g/kg bw (U1). However, the number of naïve T cells, CD8+CD62L+ in mice with digestive allergy after administration of purple yam tuber ethanol extract increased significantly in lower dose (0.167 g/kg bw (U1) compared with positive control (OVA). Dose variations of extract ethanol of purple yam tuber (0.167 g/kg bw) has a significantly effect to shift the T cell status from memory to naïve. Keywords: Digestive allergy, Dioscorea alata L., histopathology, immunomodulatory, subset T cells

Effects of Fish Oil and Tomato Powder Supplementation in Mojosari Ducks Diet on Egg Quality Faizal Andri; Eko Widodo; Irfan Hadji Djunaidi
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

This research aimed to examine the effects of fish oil and tomato powder supplementation on egg quality of Mojosari ducks. A total of one hundred and forty Mojosari ducks (consisted of 20 male and 120 female ducks), aged at 40 weeks old, were randomly allotted into 20 flocks (each flock consisted of 1 male and 6 female ducks). Method used was experiment in a Completely Randomized Design with 5 treatments and 4 replications. Dietary treatments used were T0: basal diet (control), T1: basal diet + 1% FO, T2: basal diet + 2% FO, T3: basal diet + 1% FO + 1% TP; T4: basal diet + 2% FO + 1% TP. Data were analyzed using one-way analysis of variance (ANOVA). Result showed that supplementation of fish oil and tomato powder did not significantly affect (P>0.05) external egg quality (egg shape index, shell surface area, shell thickness and specific gravity). Dietary treatments also had no significant effect (P>0.05) on internal egg quality (albumen volume, yolk volume, albumen index, yolk index and Haugh unit). It is concluded that there was no detrimental effect of fish oil and tomato powder supplementation in Mojosari ducks diet on external and internal egg quality.Keywords: antioxidant, Haugh unit, laying duck, omega-3 fatty acids, poultry.

Effect of Five Types Inert Dust to Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae) in Stored Rice Seeds Astuti, Ludji Pantja; Maula, Rohmatin; Rizali, Akhmad; Mario, Muhammad Bayu
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Rhyzopertha dominica (F.) is one of the primary pests in stored rice. Generally, pest control in the storage was done by spraying and fumigation using synthetic insecticide. The application of chemical insecticide using phosphine can cause resistant to insect pests and toxic to humans. Regarding the negative effect of insecticide application, the alternative control by using natural resources like inert dust is expected to be a good solution to control pests of stored products. This research was aimed to study the effectiveness of five inert dusts, which are: rice husks ash, volcanic ash, giant bamboo leaves ash, corncobs ash, coconut shells ash to adult mortality and the inhibition of population growth of R. dominica on stored rice seeds. The results showed that giant bamboo leaves at 8 g kg-1 caused 100% of mortality and faster than other inert dusts tested. Giant bamboo leaves and rice husks were more effective to suppress the number of eggs, larvae, pupae, and new adults (F1). Inert dust could decrease the hatchability of eggs, inhibit population growth, and decrease the weight of new adults (F1). The damage to the treated rice seeds was significantly lower than untreated rice seeds. Keywords: ash, control, inert dust, silica, stored product pest

Penggunaan Kultur Starter Bakteri Asam Laktat pada Pengolahan Sosis Fermentasi Ikan Lele Dumbo yang Diinfeksi Listeria monocytogenes ATCC-1194 Happy Nursyam
The Journal of Experimental Life Science Vol. 1 No. 2 (2011)
Publisher : Postgraduate School, Universitas Brawijaya

Penggunaan biopreservatif bakteri asam laktat pada bahan makanan sangat efektif dalam mengontrol pertumbuhan bakteri patogen dan mikroorganisme pembusuk. Bakteri asam laktat pada produk fermentasi, selain berperan sebagai biopreservatif juga berperan penting dalam meningkatkan kualitas nutrisi bahan mentah yang difermentasi. Penelitian ini merupakan kajian tentang penggunaan kultur starter Pediococcus acidilactici; Lactobacillus casei; dan kombinasi Pediococcus acidilactici dan Lactobacillus casei; serta tanpa starter kultur sebagai kontrol, terhadap karakter biopreservatif sosis fermentasi ikan lele dumbo yang diinfeksi Listeria monocytogenes selama pematangan 28 hari pada suhu inkubasi 15-22°C. Berdasarkan hasil penelitian diketahui komponen biopreservatif yang dihasilkan didominasi oleh senyawa alkohol, keton, asam-asam lemak, ester dari asam lemak, fenol, benzene, dan senyawa volatil lain. Fenol merupakan senyawa yang terbanyak. Semakin besar rasio C15:0/C17:0 dalam sosis fermentasi ikan lele dumbo, pertumbuhan Listeria monocytogenes makin sedikit. Sosis yang difermentasi menggunakan kombinasi Pediococcus acidilactici dan Lactobacillus casei starter memiliki rasio C15:0/C17:0 terbesar, dan mampu mematikan pertumbuhan Listeria monocytogenes. Rasio C15:0/C17:0 dengan nilai 79,84 merupakan dosis yang mematikan bagi Listeria monocytogenes pada suhu inkubasi 15-21,2°C secara in vitro. Kata kunci: BAL, biopreservatif, Ikan Lele Dumbo, Listeria monocytogenes

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Jehan Ramdani Hariyati

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Home Page

OAI Link

Editorial Team

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Reviewer

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Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

Journal Mail Official jehanramdani@ub.ac.id

Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA