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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 299 Documents

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Extract of Caesalpinia sappan L. as Antibacterial Feed Additive on Intestinal Microflora of Laying Quail Anang Widigdyo; Eko Widodo; Irfan Hadji Djunaidi
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Caesalpinia sappan L. is a phytobiotic plant that serves as an antibacterial. Active compound such as flavonoids in the C. sappan L. extract acts as an antibacterial. TheÂ protein food sources in Indonesia including poultry such as quail. Several studies demonstrated that quail are very susceptible to bacterial infections including Escherichia coli and Salmonella. Thus C. sappan has been used as food additive for quail in laying eggs. This research objective was to study the effects of C. sappan L. extract against intestinal miclofora of quail in laying eggs. This study used 168 quails with 4 treatments and 5 replications, each treatment used 7 quails. This study used 4 Wood Extract C. sappan L. treatment with different concentrations including 0%, 0.2%, 0.4%, 0.6%. The results showed that administration of Extract Wood C. sappan L. was not significantly different (P> 0.05) to the total number of bacteria E. coli with the average P0 (6.0903 Log CFU), P1 (6.0903 log CFU), P2 (6.0887 Log CFU), and P3 (6.0868 Log CFU). Meanwhile, it was significantly different (P <0.05) to the total number of Salmonella bacteria in the gut laying with the average number of bacteria P0 (5.4059 Log CFU), P1 (5.4048 Log CFU), P2 (5.4045 Log CFU), and P3 (5.4039 Log CFU). It is concluded that flavonoids compounds in C. sappan L. extract could decrease the number of Salmonella, but not E. coli, in the intestine of quail.Keywords: Caesalpinia sappan L., Eschericia coli, Flavonoid, Salmonella.

Phytochemical Screening and Antimicrobial Activity of Roselle (Hibiscus sabdariffa L.) Flower Extract Against Aeromonas hydrophila Sitti Khairul Bariyyah; Arief Prajitno; Ating Yuniarti
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Medicinal plants as an antimicrobial agent may provide an alternative way to replace the use of antibiotics to control disease agents in aquaculture. Roselle flowers (Hibiscus sabdariffa L.) has been used in many sectors as a source of functional food, natural coloring agents as well as antimicrobial agents. The objectives of this study were to evaluate the phytochemical compound in methanolic extract of roselle flower and their antimicrobial activity against Aeromonas hydrophila. The phytochemical composition of roselle flower was evaluated using phytochemical screening and FTIR. While the antimicrobial activity was performed by using the disc diffusion agar and co-culture with A. hydrophila. The results of phytochemical screening confirmed the presence of alkaloids, flavonoids, saponins, steroids, triterpenoids, and tannins. The results of FTIR revealed that Roselle flower extract had the main phenolic compounds. The result of disc diffusion and co-culture method indicated that the roselle flower extract had antibacterial activity against A. hydrophila. This antibacterial activity depended on the concentration applied.Keywords: antibiotics, Co-culture, Disk diffusion, flavonoid, Hibiscus sp.

Effect of Maintenance at Different Salinity against White Spot Syndrome Virus (WSSV) Infection Level in Post Larvae Litopenaeus vannamei Shrimp Attabik Mukhammad Amrillah; Sri Widyarti; Yuni Kilawati
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

White spot syndrime virus (WSSV) is a highly contagious disease in shrimp culture and causing 100% death within 3-10 days of clinical symptoms. WSSV can infect shrimp in post larvae stage (PL) to a size of 40 g. This study was aimed to determine the effect of different salinity against WSSV infection level in post larvae vannamei shrimp (Litopenaeus vannamei). In this study PL 40 size of vannamei shrimp infected with WSSV in virus concentration 20 μg.mL-1 in three different salinity is 5 ppt, 15 ppt, 25 ppt for 4 hours using the soaking method then maintened for 7 days post-infection and then infection level and water quality observed. Data analysis used simple linear regression and F test with a confidence level 95% (P <0.05). The results indicate changes in behavior between healthy and infected shrimp in three levels (mild, moderate, severe). The highest total mild infection in salinity treatment of 25 ppt by 30%, the highest total medium infection at salinity treatment 25 ppt by 37%, the highest total severe infection at salinity treatment 5 ppt by 57%. Shrimp that have mild level of infection are increasing in concominant higher salinity, while the severe infection level has decreases with increasing salinity. Keywords: Infection Level, Litopenaeus vannamei, Post Larvae, Salinity, WSSV

Biological Analysis of Blue Swimmer Crab (Portunus pelagicus) from Collectors in Lamongan, Tuban, Pasuruan, and Rembang, Java, Indonesia Lini Murni; Diana Arfiati; Andi Kurniawan; Yuni Kilawati; Herwati Umi Subarijanti; Suliswanto Suliswanto; Muhammad Nafar Amani Syams; Agum Bayu Gumelar; Alif Raditya Amirul Huda
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Blue swimmer crab (Portunus pelagicus) can increase the country's incomes due to its high demand of export. This study aims to determine biological analysis on the population of blue swimmer crab in Lamongan, Tuban, Pasuruan, and Rembang. The sampling location was based on the fishing area and the method used survey method from collectors in each location. The blue swimmer crab's biological characteristics which observed are the correlation on wide of carapace and blue swimmer crab weight, sex ratio, and gonad maturity level. The results showed the correlation between the wide and weight of carapace in Lamongan (male: 113.80-122.99 mm and 87.98-121.60 g then female: 108.21-117.27 mm and 71.80-99.74 g), Tuban (male: 113.26-117.74 mm and 89.63-102.76 g g then female: 114.36 mm and 106.51 g), Pasuruan (male: 87.66-94.43 mm and 39.68-49.90 g then female: 101.94-110.89 mm and 66.98-82.89 g), and Rembang (male: 120.30-126.69 mm and 93.53-118.43 g then female: 125.30-132.13 mm and 134.42-157.47 g); sex ratio (male: female) in Lamongan (1.4: 1), Tuban (1: 1.5), Pasuruan (2.8: 1), and Rembang (1: 1.46); and also gonad maturity level in Lamongan (dominant male at level III was 30.29%, female at level II and III was 30.40%), Tuban (dominant male at level II was 41.93%, female at level III was 33.33%), Pasuruan (dominant male at level III was 25.79%, female at level III was 48.10%), and Rembang (dominant male at level II was 32.35%, female at level II was 33.33%). These results indicate that the total of Portunus pelagicus which was being caught had fulfilled the standards rules by No.1/PERMEN-KP/2015. However, it requires continuous direct monitoring and counseling to maintain the stock of blue swimmer crab resources. Keywords: Blue swimmer crab (Portunus pelagicus), gonad maturity level, sex ratio, wide and weight.

Hibiscus Sabdariffa Linn) terhadap NF-ĸβ, TNF-α dan ICAM-1 pada Human Umbilical Vein Endothelial Cells (HUVECs) Cultured yang dipapar Low Density Lipoprotein (LDL) Teroksidasi Dwi Sarbini; Djanggan Sargowo; Muhammad Saifur Rohman
The Journal of Experimental Life Science Vol. 1 No. 2 (2011)
Publisher : Postgraduate School, Universitas Brawijaya

Tujuan penelitian ini adalah mengetahui efek dan mekanisme kerja ekstrak teh Rosella merah (Hibiscus sabdariffa Linn) terhadap aktifasi NF-ĸβ dan ekspresi protein TNF - α serta ICAM-1 yang menjadi mediator inflamasi pada aterosklerosis. Penelitian ini menggunakan kultur sel endotel yang diisolasi dari vena umbilikalis manusia ( HUVECs). Kelompok kontrol digunakan HUVECs tanpa paparan ox-LDL (kontrol negatif) dan HUVECs yang dipapar 40 µgml-1 Ox-LDL (kontrol positif). Kelompok perlakuan adalah HUVECs yang dipapar dengan berbagai dosis teh Rosella merah (0,01 mgml-1, 0,005 mgml-1 dan 0,001 mgml-1) dan diberikan selama 2 jam sebelum dipapar ox-LDL. Pengukuran aktifasi NF-ĸβ dilakukan setelah 30 menit paparan Ox-LDL menggunakan imunohistokimia. Ekspresi protein TNF-α dan ICAM-1 diukur setelah 24 jam dipapar Ox-LDL menggunakan imunohistokimia. Berdasarkan analisis ANOVA (p<0.01) terdapat efek penghambatan ekstrak teh Rosella merah (Hibiscus sabdariffa Linn) terhadap aktifasi NF-ĸβ dan ekspresi protein TNF -α serta ICAM-1 yang manjadi mediator terjadinya inflamasi pada aterosklerosis melalui penghambatan aktifasi NF-ĸβ. Terdapat hubungan negatif antara aktifasi NF-ĸβ dan ekspresi protein TNF -α serta ICAM-1 dengan dosis ekstrak teh Rosella merah (Analisis Spearman's [p<0,01, Correlation Coeff = -1]). Kata Kunci: atherosklerosis, ICAM-1, NF-ĸβ, Ox-LDL, Rosella merah (Hibiscus sabdariffa Linn), TNF-α

Dynamical Analysis of Fractional-Order Hastings-Powell Food Chain Model with Alternative Food Moh Nurul Huda; Trisilowati Trisilowati; Agus Suryanto
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

In this paper, a fractional-order Hastings-Powell food chain model is discussed. It is assumed that the top-predator population is supported by alternative food. Existence and local stability of equilibrium points of fractional-order system are investigated. Numerical simulations are conducted to illustrate analysis results. The analysis results show that alternative food can give a positive impact for top-predator population.Keywords: Alternative food, Fractional-order, Grunwald-Letnikov approximation, Hasting-Powell model, Stability.

Effects of Monosodium Glutamate Oral Administration on LH and Testosterone Levels in Serum of Adult Male Rats (Rattus norvegicus) Riska Annisa; Moch. Sasmito Djati; Sri Rahayu
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

This study aims to determine the effect of monosodium glutamate on LH and testosterone levels in serum of adult male rats (Rattus norvegicus). A total of 12 male Wistar-strained rats divided into three groups with 4 rats per group. Control group was given distilled water, while M1 was given MSG at a dose of 4mg g-1 BW for 15 days and M2 was given MSG at a dose of 4 mg g-1BW for 45 days. All substances were given orally. At the end of the study, rats were sacrificed with a dislocation to draw blood from the heart. Then, the blood was incubated at room temperature to obtain serum. Serum was used to analyze LH and testosterone levels using the ELISA kit. Data were analyzed using one way ANOVA (p ≤ 0.05) with SPPS 16.0 for windows. The results showed that serum LH levels after MSG treatments for 45 days was significantly decreased compared with the control. The serum testosterone levels M2 was significantly difference with the control group (p ≤ 0.01). This study predicts that monosodium glutamate have a direct and indirect effect on male reproductive organs. Keywords: Luteinizing hormone, Monosodium glutamate, testosterone.

Identifikasi Protein Adhesi Pili Proteus Mirabilis P355 dan Protein Reseptor pada Vesika Urinaria Kelinci Dian Chusna Mufida; Sumarno Sumarno; Sanarto Santoso
The Journal of Experimental Life Science Vol. 1 No. 1 (2011)
Publisher : Postgraduate School, Universitas Brawijaya

Infeksi saluran kemih (ISK) merupakan infeksi nosokomial yang sering terjadi di rumah sakit. Penyebab ISK nosokomial ini diantaranya adalah Proteus mirabilis p355 dan sering dijumpai pada pasien yang memakai kateter. Proteus mirabilis mempunyai berbagai macam faktor virulensi, diantaranya adalah fimbria atau pili. Tujuan penelitian ini untuk mengidentifikasi protein pili Proteus mirabilis (molekul adhesin) serta mencari protein reseptor bakteri tersebut pada epitel vesika urinaria kelinci. Penelitian ini dilakukan dengan isolasi protein pili, selanjutnya dilakukan uji hemaglutinasi dan uji hambat adhesi. Uji hambat adhesi mempergunakan protein pili yang telah dielektroelusi dan didialisa, disalutkan pada sel epitel vesika urinaria kelinci dengan dosis 400 µl, 200 µl, 100 µl, 50 µl, 25 µl, 12,5 µl dan 0 µl sebagai kontrol. Selanjutnya dilakukan identifikasi protein reseptor dengan cara menyalut bakteri Proteus mirabilis dengan matriks ekstra seluler dari epitel vesika urinaria kelinci. Hasil penelitian ini menunjukkan adanya reaksi hemaglutinasi dari protein pili dengan berat 35,2 kDa dengan titer tertinggi 1/521. Hasil uji hambat adhesi epitel vesika urinaria yang disalut protein pili dengan berat 35,2 kDa menunjukkan bahwa semakin tinggi dosis protein yang diberikan, semakin sedikit bakteri yang melekat pada epitel vesika urinaria. Penurunan ini terjadi secara signifikan dengan indeks regresi (r) = 0,95 dan p-value = 0,00. Berdasarkan penelitian ini dapat disimpulkan bahwa protein pili Proteus mirabilis dengan berat molekul 35,2 kDa merupakan protein adhesin, sedangkan protein reseptor Proteus mirabilis diprediksi mempunyai berat molekul, 36 kDa, 24,5 kDa dan 24 kDa. Kata kunci : adhesi, pili, protein reseptor, Proteus mirabilis

Potency of Green Cincau Leaves (Premna oblogifolia merr) Juice as Prebiotics and its Effect on Laying Hen Performances Anna Lidiyawati; Eko Widodo; Edhy Sudjarwo
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

This study investigated the potency of green cincau leaves (Premna oblongifolia merr) juice as prebiotics end its effect on laying performances (feed intake, egg weight, feed conversion and hen day production) based on pH and digestive viscosity. The potency of green cincau leaves juice as prebiotic was tested. The result showed that green cincau leaves juice was effectively support growth of Lactic Acid Bacteria (LAB) with 4.3 x 102 CFU.g-1 samples but did not support the growth of Escherichia coli. The effect of green cincau leaves juice on laying performances was examined. Two hundred fifty 56-wk-old Hisex Brown layers received different diets namely 0, 5, 7.5, 10 and 12.5 ml of green cincau leaves juice added in the basal feed. The method used in this study was experiment based on Completely Randomized Design and followed by Duncanâ€™s Multiple test if significant effect occurs. The result showed that increase level of P. oblongifolia merr in feed did not significantly affect (P>0.05) feed intake, egg weight, feed conversion and hen day production.Keywords: feed additives, laying hens performance, prebiotic, Premna Â oblongifolia merr juice.

Immunostimulant Activity of Neem Leaf (A. indica A. Juss) Ethanol Fraction on Tilapia (Oreochromis niloticus) Rika Putri; Sri Andayani; Ating Yuniarti
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Intensive cultivation of Tilapia with high stock density and continuous artificial feeding will decrease the water quality and increase the growth of pathogenic bacteria. That situation led to the fish stressed so that the fish become the target of bacterial pathogen attacks. Alternatively, the environmental friendly treatment using herbal immunostimulant to solve that problem is required. The herbal in this study used neem leaf (Azadirachta indica A. Juss). The aim of this study was to evaluate ethanol fraction of neem leaf on immunostimulant activity of Oreochromis niloticus. In this study, the extraction method used maceration. Fractionation methods were using Thin Layer Chromatography (TLC) and Column Chromatography (CC). The biological component was analyzed using Phytochemical, FTIR and GC-MS methods. TLC test showed that ethyl acetate: n-hexane (1:1, v/v) result in brightest luminescence colors on the plates of silica gel under UV light (365 nm). The chromatography column from the ethanol extract of neem leaf (A. indica A. Juss) showed that separation of the fraction was done based on the formation of color, which obtained 5 (five) types of fractions. The results of the phytochemical test for the fraction 4 of neem leaf contains two compounds, i.e. flavonoids (+) and triterpenoids (++). The FTIR test showed that fraction 4 of neem leaves contain a class of terpenoid and flavonoid compounds. GCMS test results on fraction 4 of the neem leaves (A. indica A. Juss) obtained the high peak, which has 4 terpenoid compounds, 1 compound of flavonoid, and 1 type of lauric acid. The total leukocyte of the fish test increased after treated with fraction 4 of neem leaf (A. indica A. Juss) compared with control on day 7 after injection, i.e. 25.29 x 104 cells.mL-1.Keywords: fraction, leukocyte, Neem Leaves, Tilapia, Phytochemicals.

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Contact Name Jehan Ramdani Hariyati

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Editorial Address Jl. Veteran Malang 65145

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA