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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 299 Documents

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Water and Chlorophyll Content and Leaf Anatomy of Patchouli Planlet (Pogostemon cablin Benth.) Resulted by Shoot-tip Culture Experience Hyperhydricity after Treatment of Modification Ammonium nitrate or Macro salt Concentration on MS medium (Murashige Skoog) Aisyah Rahmawati Zen; Wahyu Widoretno; Serafinah Indriyani
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Hyperhydricity is a symptom of abnormal morphological and physiological function which inhibits the regeneration of plantlets. In general, the main symptom of hyperhydricity is a change in the condition of the plantlets which looks clear (Glassy) as a result of low levels of chlorophyll, the high water content in the plantlets, and the abnormal anatomical structure of the leaves. Hyperhydricity can be controlled by reducing cytokinin concentration, increasing gelling agent concentration, and reducing ammonium nitrate and macro salt concentration on medium. Objective of this research was to reduce hyperhydricity in shoot tip culture of patchouli by modification of ammonium nitrate and macro salt concentration on MS medium. The various treatment concentrations of ammonium nitrate were 0 mg.L-1 (0), 41.25 mg.L-1 (¼ concentration), 825 mg.L-1 (½ concentration), 1650 mg.L-1 (1 concentration) and macro salt MS with 0, ¼ MS, ½ MS, MS with 5 replications. Hyperhydricity on patchouli shoots could be lowered, as indicated by the decrease in water content from 96% to 90-91%, the increase in total chlorophyll content, and the increased number of palisade cells and stomata on the leaf treatment outcome. The concentration treatment of ammonium nitrate showed better results than the concentration of macros salt in increasing the total chlorophyll content, but it did not differ significantly in lowering water levels and increasing the number of palisade cells and stomata. ¼x concentration treatment of ammonium nitrate could increase chlorophyll content of 0.16 to 0.97 mg.g-1, but MS with 1x concentration showed the best result in the increase of number of palisade cells and stomata of the leaves. Keywords: Ammonium nitrate, Hyperhydricity, Macro Salt, Shoot-tip culture.

Comparing Vector-host and SEIR models for Zika Virus Transmission Puji Andayani; Rizal Dian Azmi; Lisa Risfana Sari
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Some mathematical models to describe Zika virus transmissions have been analyzed. In this study, we construct two models of Zika virus transmission. The first one is the vector-host model. It considers the human population as host and mosquito's population as the vector. The second model is where there is only infected human population who act as transmitter without the existence of infected mosquitoes in the population. The impact of modeling assumption of Zika virus is studied by analyzed the reproduction number using Next Generation Matrix (NGM) method. Formerly, we compare the dynamics of the two models by interpreting the reproduction number of each model. Biologically, the two models cause a similar effect. If the reproduction number is less than one, then the disease is extinct. Otherwise, an endemic condition exists. The numerical simulation also used to explain the comparison of two models. The recovery and the transmission period are solved to compare these two cases. Keywords: comparison, mathematical model, reproduction number, SEIR, Zika Virus.

Effects of Cadmium Exposure on Lipid Peroxidation and Chlorinative Stress of Rat Kidney Eko Suhartono; Triawanti Triawanti; Amin Setyo Leksono
The Journal of Experimental Life Science Vol. 5 No. 1 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

In addition to a wide range of adverse effects on human health, toxic metals such as cadmium (Cd) can promote kidney damage. In this study, we proposed the toxicological properties of these metals which are partly related to lipid peroxidation and chlorinative stress. Thus our study aimed to measure the lipid peroxidation and chlorinative stress in kidney of rats exposed to Cd. The study was performed on 32 male rats (Rattus novergicus) weighing 300±10 grams with normal activity. Rats were acclimatized for a week before the treatment. The animals were divided into two major groups with one group for control (K0) and the other groups were exposed to 3 mg/L CdSO4 through the drinking water. The animals in each groups were divided again into two small groups with different time of exposure. The time of exposure are subacute (six weeks) and subchronic (eight weeks). After the exposure the kidney from all treated animals showed the significant increase of MDA and AOPP levels. In conclusion, subacute and subchronic exposure of Cd caused the kidney damage through lipid peroxidation and chlorinative stress, as shown by increasing of MDA and AOPP levels. Keywords: AOPP, Cadmium, Chlorinative Stress, Lipid Peroxidation, MDA

Dynamical Analysis of HIV/AIDS Epidemic Model with Treatment Badria Ulfa; Trisilowati Trisilowati; Wuryansari M. K.
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

In this article, an epidemic model of HIV/AIDS with treatment is observed. This model consists of five populations: susceptible, educated susceptible, exposed, HIV infected, and AIDS infected. Antiretroviral therapy (ART) is one type of treatment that can be given to individual that is infected by HIV. This medication can prevent the growth of the virus. Exposed individuals are given short-term antiretroviral treatment called Post Exposure Prophylaxis (PPP), while for infected individuals are given treatment by combining two or three types of antiretroviral drugs. Dynamical analysis is performed by determining equilibrium points and local stability analysis. Based on the analysis results, two equilibrium points are obtained, namely disease-free equilibrium point and endemic equilibrium points. The stability analysis shows that the free equilibrium point is locally asymptotically stable if R0 < 1 and the endemic equilibrium point is locally asymptotically stable under certain conditions. Numerical simulations show that giving two medications together has a greater effect in reducing the spread of the disease. Keywords: antiretroviral, dynamical analysis, HIV/AIDS.

Mobilization of CD4+, CD8+, and B220+ on Broiler Chicken Spleen with Feed Contained Polyscias obtusa Post Infection of Salmonella typhimurium Andi Rizki A, Pradana; Muhammad Sasmito Djati; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 3 No. 1 (2013)
Publisher : Postgraduate School, Universitas Brawijaya

Farmers in Indonesia used to provide feed with additional antibiotics to prevent the endemic disease in poultry such as New Castle Disease and Avian Influenza. This has a negative impact because the antibiotic residues will accumulate in meat and harmful to consumers. The aim of this research was to determine the role of simplicia Polyscias obtusa leaves as immunostimulants in broiler feed that had been infected with Salmonella typhimurium. Sixty of DOC (days old chicks) with initial weight 37 g were used in this experiment. The broilers were offered diets containing different levels of simplicia leaves of as follows; 0% with no infection (A1), 0% (A2), 0,08% (A3), 0,16% (A4), and 0,26% (A5). At day 14th, the broiler orally infected with 108 CFU/ml Salmonella typhimurium, 500 µl for each broiler. Treatments were allocated in a completely randomized design. The variable observed were the relative number of lympohcyte cell CD4+, CD8+, and B220+ of spleen analyzed by flowcytometry. The results obtained showed that additional simplicia Polyscias obtusa leaves in feed can significantly affect the development (relative number) of lymphocytes cell, especially T cells CD8+. Treatment A4 (P. obtusa 2nd dose (0.16%) + S. typhimurium infection) had the best ability to increase the relative number of lymphocytes cell. These result strengthens Polyscias obtusa role as one of immunostimulatory agent, in terms of its active compounds (saponins and flavonoids). Keywords : Broiler, immunostimulants, lymphocyte cell, Polyscias obtusa, spleen.

The Effect of Treatment Ant Nest (Myrmecodia pendans) on Quail Production and Performance (Coturnix-coturnix japonica) Susan Elfitri Lumban Gaol; Edhy Sudjarwo; Irfan Hadji Djunaidi
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

This research was aimed to determine the effect of ant nest treatment (Myrmecodia pendans) on quail production and performance. Research sample was 250 quails. Ant nest was administered in 5 treatments and 5 replications: P0 (0%) as control treatment, P1 (0.2%), P2 (0.4%), P3 (0.6%), and P4 (0.8 %) treatments. Data were collected from quails at 28 days to 70 days old. Variables measured were the performance and quail's egg production. This study was designed using completely randomized design. Real significant difference was examined utilizing Duncan's Multiple Range Test (DMRT). Analysis of variance result showed that ant nest provides significant influence to feed intake and Hen Day Production (P <0.05). The highest average of feed consumption is treatment P4 (0.8%), whereas treatment P0 (0%) was the lowest average for feed consumption. Feed conversion rate in treatment P4 (0.8%) was more efficient compared to other treatments. Ant nests administering did not result significantly different (P> 0.05) to feed conversion and egg mass. The results of the study concluded that higher ant nest administration is capable of increasing quails feed intake and Hen Day Production. Keywords: basal feed, flavonoids, productivity.

Antifeedant Effect of Moringa oleifera (L.) Leaf and Seed Extract on Growth and Feeding Activity of Spodoptera litura (Fab.) (Lepidoptera: Noctuidae) Amelia Tridiptasari; Amin Setyo Leksono; Dian Siswanto
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

This study was conducted to evaluate the effect of Moringa oleifera leaf and seed extract on length, weight, and feeding activity of Spodoptera litura. Leaf and seed were extracted by maceration method in 70% ethanol. Bioassays were conducted by using five concentrations (0%, 5%, 10%, 15%, 20%) on 5 larvae by leaf-dip method with 5 times replication. The result showed that leaf and seed of Moringa extract contained alkaloid, flavonoid, and saponin which gave antifeedant effect to growth and feeding activity of larvae. The increased extract concentrations are lead to weight loss and the length shrinkage of larvae. The extracts promoted prolongation of the larval instar. Reduction of food intake of the larva is in line with the concentration increase of extracts. The antifeedant effect also affects time to stop feeding and give mortality of larvae.Keywords: antifeedant, leaf, moringa, seed, S. litura

The Effect of Gibberellin on Somatic Embryo Growth and Maturation and Plantlet Regeneration of Tangerine (Citrus reticulata Blanco.) var. Batu 55 Elok Rifqi Firdiana; Serafinah Indriyani; Wahyu Widoretno
The Journal of Experimental Life Science Vol. 5 No. 1 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

The effect of gibberellin at multiplication stage on somatic embryo growth and maturation and plantlet regeneration of tangerine (Citrus reticulata Blanco.) var. Batu 55 was assessed. Somatic embryo at globular phase was cultured on MT media + 30 gL-1 sucrose and various concentrations of gibberellin (0, 2, 4, 6, and 8 ppm). The somatic embryo was maturated on MT media + 500 ppm malt extract + 73 mM sorbitol + 73 mM galactose. Cotyledonary somatic embryo was regenerated into plantlet on MT media + 500 ppm malt extract + 30 gL-1 sucrose + 2 ppm GA3. The results showed that the addition of gibberellin in somatic embryo multiplication stage increased somatic embryo growth and maturation and plantlet regeneration of tangerine. Optimum concentration of gibberellin needed for somatic embryo growth was 4 ppm which yielded two-fold fresh weight compared to control. The percentage of maturation was very low below 5%. The addition of gibberellin in media at embryo multiplication stage slightly increased the percentage of maturation about 1-2%. Thirty percent of cotyledonary embryo was able to regenerate into plantlet. The addition of gibberellin in media at embryo multiplication stage increased the regeneration percentage, even the addition of 8 ppm gibberelline yielded regeneration percentage up to 70%.Keywords: gibberellin, growth, maturation, plantlet regeneration, somatic embryo

Numerical Solution of a Fractional-Order Predator-Prey Model with Prey Refuge and Additional Food for Predator Rio Satriyantara; Agus Suryanto; Noor Hidayat
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

In this paper, a fractional-order predator-prey model with prey refuge and additional food for predator is solved numerically. For that aim, the model is discretized using a piecewise constant arguments. The equilibrium points of the discrete fractional-order model are investigated. Numerical simulations are conducted to see the stability of each equilibrium point. The numerical simulations show that stability of the equilibrium points is dependent on the time step.Keywords: Additional Food, Fractional-Order, Predator-Prey, Prey Refuge.

Isolasi dan Karakterisasi Protein 100kDa dari Membran Kepala Spermatozoa Kambing Bayyinatul Muchtaromah; Sutiman Bambang Sumitro; Soemarno Soemarno; Trinil Susilawati
The Journal of Experimental Life Science Vol. 2 No. 1 (2012)
Publisher : Postgraduate School, Universitas Brawijaya

Antigen adalah protein yang dapat menginduksi terbentuknya antibodi. Berat molekul protein sebesar 100 kDa dapat bertindak sebagai imunogen yang kuat. Tujuan dari penelitian ini adalah untuk mencari kandidat vaksin imunokontrasepsi dan melakukan isolasi serta karakterisasi protein 100 kDa yang diduga mempunyai peranan penting di dalam interaksi spermatozoa-ovum. Penelitian ini merupakan penelitian eksploratif. Materi Protein 100 kDa diisolasi dari membran spermatozoa kambing menggunakan detergen N-Octyl-glycopiranoside kemudian dirunning menggunakan metode SDS-PAGE dan dikoleksi dengan elektroelusi. Karakterisasi Protein 100 kDa meliputi penentuan berat molekul melalui SDS-PAGE, penentuan titik isoelektrik (pI) dengan IEF (isoelectric focusing) dan kandungan protein dengan metode Biuret. Hasil running isolat protein membran spermatozoa kambing menggunakan metode SDS-PAGE diperoleh pita protein yang terdiri atas 7 pita dengan berat molekul 166,7; 122; 100; 82,8; 60,4; 29,9 dan 14,8 kDa. Isolat Protein 100 kDa diperoleh dengan purifikasi menggunakan metode elektroelusi. Hasil IEF gel elektroforesis Protein 100 kDa dari membran spermatozoa kambing yang diejakulasikan ini mempunyai tiga titik isoelektrik yaitu 6,37; 6,05 dan 5,64. Kandungan protein dalam isolat Protein 100 kDa sebesar 2375 ± 5,65 µg/mL. Hasil ini menunjukkan bahwa isolat protein 100 kDa dapat digunakan sebagai imunogen. Kata Kunci : isolasi, karakterisasi, membrane spermatozoa, protein 100kDa

Page 8 of 30 | Total Record : 299

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Home Page

OAI Link

Editorial Team

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Reviewer

Google Scholar

Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

Journal Mail Official jehanramdani@ub.ac.id

Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA