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INVENTORY OF WILD PLANT TYPE OF TRADITIONAL MEDICINE MADURA SUMENEP COMMUNITIES Ismawati Ismawati; R. Amilia Destryana
SIMBIOSIS Vol 7 No 2 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Sumenep is one of the regencies on Madura Island which is famous for traditional herbal medicine. The traditional herbal medicine ingredients of the Sumenep community come from wild plants and cultivation plants. The research aims to identify wild plant species as an effort to inventory wild plants that are used as traditional herbal ingredients. The research is an exploratory study conducted by in-depth interviews with sources who understand traditional herbal medicine such as traditional birth attendants, masseurs and herbalists. The study was conducted in District of Dungkek, Batang-Batang and Gapura. The results found 22 families of wild plants consisting of 29 types of plants with diverse benefits as ingredients of traditional herbal medicine.

POTENTIAL OF Pseudomonas sp. AND Ochrobactrum sp. ISOLATED FROM VARIOUS SOIL SAMPLE AS DEGRADING BACTERIA OF HIGH DENSITY POLYETHYLENE (HDPE) AND LOW DENSITY POLYETHYLENE (LDPE) PLASTIC Muhammad Inas Riandi; Retno Kawuri; Sang Ketut Sudirga
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

plastics on a large scale has an effect on the increase to the amount of world waste, such as High Density Polyethylene (HDPE)and Low Density Polyethylene (LDPE) plastics. This study, tried to isolate Pseudomonas and Ochrobactrum bacteria from varioussoil in dumped soil area and mangrove forest in Bali to find out the degradation ability of the bacteria against HDPE and LDPEplastics. The methods used in this study were pour plate method, Biochemical and morphology identification by using Bergey’sManual of Determinative Bacteriology 9th Edition book as a reference, screening test and the determination of bacterial species byusing Vitek 2 Compact System. Evaluation of degradation result was done by measuring the weight loss of plastics residues. Thisstudy has isolated 27 bacteria that estimated from genus Pseudomonas and Ochrobactrum with characteristics as basil shape, Gramnegative, positive catalase and classified as non-fermenter bacteria. Isolate 2 bacteria which isolated from Suwung dumped soil hasidentified as Ochrobactrum anthropi with the ability of HDPE degradation by 20% and Isolate 24 which isolated from Karangasemdumped soil has identified as Pseudomonas aeruginosa with the ability of HDPE degradation by 18,75%.Keywords : Plastics waste, Biodegradation, High Density Polyethylene and Low Density Polyetene

ISOLASI, IDENTIFIKASI DAN UJI POTENSI BAKTERI YANG BERPERAN PADA PENGOLAHAN AIR LIMBAH YANG MENGANDUNG RHODAMIN B DALAM BIOSISTEM TANAMAN Sang Ayu Sri Satya Laksmi Utari; Ida Bagus Gede Darmayasa; I Wayan Budiarsa Suyasa
SIMBIOSIS Vol 3 No 1 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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Rhodamin B is a dangerous synthetic dyes substances used as a fabric dye in thetextile industry. Biodegradation is one way that is used in the processing of liquid wasteRhodamin B. Biodegradation technique is leveraging the ability of microbial activitiesdegrade or eliminate pollutant compounds. The main objective of this research was toinvestigate the characteristic of bacteria that are capable of living on wastewater containingRhodamin B and the effectiveness of single and microbial consortium isolates to degradeRhodamin B on wastewater. The isolation of the bacteria was done by plating method and theidentification of the bacteria by using a Kit API 20E. Test of bacterial potential of RhodaminB dyes substance carried out in the Microbiology Laboratory, Biology Department, Facultyof Science, Udayana University. It was found in this research that five bacterial isolates wereobtained (Pseudomonas sp., Shigella spp., Stenotrophomonas sp., Pasteurella sp. dan Proteussp.). Pasteurella sp. had the highest percentage degrade effectiveness of 40.55%. Microbialpotential degrade Rhodamin B by Pasteurella sp. dan Proteus sp. results showed significantdifferences (P <0.05) to control.Keyword: bacteria, biodegradation, Rhodamin B

SELEKSI JENIS TUMBUHAN PAKAN DAN KANDUNGAN NUTRIEN JENIS TUMBUHAN YANG DIMAKAN SAPI BALI (Bos sondaicus) LEPAS SAPIH DI DAERAH BUKIT BADUNG SELATAN, KABUPATEN BADUNG, BALI I Wayan Heri Dismawan; I Ketut Ginantra; Ni Luh Ni Luh Suriani
SIMBIOSIS Vol 2 No 2 (2014)
Publisher : Jurusan Biologi Universitas Udayana

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This study was conducted to determine the plants species and nutrient content of plantspecies selected by weaning of bali cattle in South Badung Hill, Badung regency, Bali. Studywas conducted in three Grazing unit Bali cattle are Sawangan, Kutuh and Pecatu. InDecember 2013 to February 2014. Determination of the composition of plant species in thehabitat (n?) is determined by the square method. Determination of plants composition in diet(r?) determined by utilization methods. This method is based on the percentage bite mark ofplants species in plot sampling. Nutrient content was analyzed crude protein, energy, calciumand phosphorus. Crude protein determination with a Semi-micro Kjeldahl technique andenergy content by Bomb Calorimeter. Determination of mineral content of calcium (Ca) andPhosphorus (P) by using Atomic Absorption Spectrophotometer (ASS). Tests carried out inthe Laboratory of Nutrition and Forage Analytical Chemistry, University of Udayana. TheJURNAL SIMBIOSIS II (2): 192- 202 ISSN: 2337-7224Jurusan Biologi FMIPA Universitas Udayana September 2014results study showed 32 species of plants available in the habitat, there are nine species ofplants that are selected by weaning bali cattle, that are Desmodium heterofilum, Pleurainterupta, Polygala glomerata, Cyperus rotundus, Desmodium triflorum, Dactylocteniumaegyptium, Portulaca sp. Polygala chinensis, and Panicum eruciforme. Nutrient content ofplant species eaten a protein ranged from 11.25% - 17.14%, GE 3.10 kcal / g - 4.11 kcal / g,Ca 0.33% - 1.86%, and 0.026% P - 0.24%.Keywords: Weaning bali cattle, plants selected species, nutrient content

COMMUNITY STRUCTURE OF SEAGRASS BEDS IN PANDAWA BEACH AQUATIC, BALI Kadek Bayu Prabha Mahesswara; Ni Luh Watiniasih; I Wayan Darya Kartika
SIMBIOSIS Vol 9 No 1 (2021)
Publisher : Jurusan Biologi Universitas Udayana

This research aimed to determine the types and structure of seagrass community based on species density, species relative density, diversity index, uniformity index, and dominance index of the seagrass. This research was conducted along Pandawa Beach, Kutuh Village, South Kuta District, Badung Regency, Bali from November to December 2019. Data of seagrass were collected from 3 line transects, 3 kuadran transects from each transect, repeated 3 times. Water quality data were collected 3 times within 4 weeks. Eight species of seagrass were identified, namely: Thalassia hemprichii, Cymodocea serrulata, Halodule pinifolia, Halodule uninervis, Halophila ovalis, Halophila decipiens, Syringodium isoetifolium, and Thalassodendron ciliatum. The highest species density found at station I that was Thalassodendron ciliatum, as well as for its relative density, and the lowest was Thalassia hemprichii. At station II and III, the highest species density was found on Cymodocea serrulata and the lowest was on Halophila ovalis. The study found that the species density of seagrasses at Pandawa Beach was categorized as high density, which dominated by Thalassodendron ciliatum at station I and by Cymodocea serrulata at station II and III. Diversity index of seagrass species at station I and II was categorized as medium species diversity and low species diversity for the station III. Dominance index of seagrass species at the three station was categorized as high dominance. Similarity index of seagrass species was categorized as medium or unstable community for the station I and II, and low similarity for the station III.

KEBERADAAN JALAK BALI (Leucopsar rothschildi Stresemann 1912) DI TAMAN NASIONAL BALI BARAT I Putu Gede Ardhana; Nana Rukmana
SIMBIOSIS Vol 5 No 1 (2017)
Publisher : Jurusan Biologi Universitas Udayana

ABSTRACT The existence of the Bali Starling (Leucopsar rothschildi Stresemann 1912) in nature is increasing anxiety, because the population of this birds is increasingly endangered due to changes in its natural habitat along with northwest coast of Bali, and are rampant illegal poaching to satisfy the demand in world market as pet birds.The purpose of this research is to examine the potential for Bali Starling in natural habitat conditions are increasingly of concern which made IUCN set critically endangered status (being Critically Endangered) since 1966. While CITES for wildlife have been included in Appendix I of the Bali Starling, meaning forbidden to be traded. The research method which was in this article used is descriptive method with primary and secondary data. Primary data were obtained from observations in the field and the secondary data were obtained from studies of the literature and the annual report of the West Bali National Park.The results showed that the potential actual population numbers of Bali Starling have yet to be ascertained. Aji W (2013) indicated that from observation in the field of Menjangan Resort and West Bali National Park found only 10 individuals, while according to Birdlife International, the amount in the remaining 49 individuals nature only (Peter R, 2015). Key words: potential, Bali Starling, extinction, natural habitat

Histological Structures of Gills of Tilaphia Fish (Oreochromis mossambicus L.) As A Water Quality Indicator In The Nusa Dua Sewage Tretment Ponds, Bali Wahyu Puji Lestari; Ngurah Intan Wiratmini; A. A. Gde Raka Dalem
SIMBIOSIS Vol 6 No 2 (2018)
Publisher : Jurusan Biologi Universitas Udayana

Nusa Dua is a popular tourist resort in Bali, where many hotels and other tourist facilities were built. In this resort, the sewage is centralized processed in a Sewage Treatment Ponds called “Unit Pengolahan Air Limbah PT ITDC” which is also often called Lagoon Nusa Dua. Nusa Dua sewage treatment ponds have 5 units of ponds, namely Pond 1A, 1B, 2A, 2B, and Pond 3. In ponds 2B and 3, tilapia fish (Oreochromis mossambicus Linn.) was raised as a water quality bio-indicator. The aim of this research was to determine the histology condition of the gill of tilapia fish that raised in the Nusa Dua sewage tretment ponds which were then used as an indicator of water quality in these ponds. The research employed a complete randomized design in which 16 samples were taken from two sampling sites with eight replications. Results of this research showed that histologic changes of the gill that were observed including edemas, fusions, and hyperplasias of secondary lamellas as well as telangiectases. Keywords: Nusa Dua sewage, Oreochromis mossambicus L., gills, histology, bioindicator

UJI KEMAMPUAN SPORA CENDAWAN MIKORIZA ARBUSKULA (CMA) LOKAL BALI PADA PERTUMBUHAN TANAMAN KEDELAI (Glycine max L.) Risha Masfufah; Meitini W. Meitini W. Proborini; Retno Kawuri
SIMBIOSIS Vol 4, No 1 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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Necessity of soybean in Indonesia increase every year along with population growth. The goverment need to importthe soybeans from abroad. Soybean in Indonesia are generally planted in ricefield and upland (dryland) which have potentialof deficiency water. Water deficiency can caused the reduction of soybean production. Therefore some alternatives are neededto increase the yield of soybean productivity, one of them is applying arbuscular mycorrhizal fungi (AMF). The aim of thisresearch was to know the dosage of isolates AMF to increase the growth of soybean. The experiments conducted over 2months at Agriculture Faculty’s Greenhouse, Udayana University. The research use Completely Randomized Design whichconsists of 5 treatments, which are : without inoculation/negative control (M0), 50 spores AMF (M1), 100 spores AMF (M2),150 spores AMF (M3), and ZA/positive control (M4). The results showed that, the inoculation of spores AMF Bali indigenuswere significantly different (P<0,05) on the number of leaves, leaf length, root fresh weight, and percentage of rootcolonization. The parameter of plant height, leaf width, plant fresh weight, and plant dry weight are no significant different(P>0,05) based on analysis of variance (ANOVA) level 5%. Doses 50 spores AMF (M1) is the dose of inoculants has beenable to increase number of leaves, leaf length, root fresh weight, and percentage of root colonization.Keywords : fungi mycorrhizal arbuscular, mycorrhiza doses, soybean.

UJI VIABILITAS BENIH ANGGREK HITAM (Coelogyne pandurata Lindl.) DENGAN MASA SIMPAN YANG BERBEDA VEMY CLAUDIA; IDA AYU ASTARINI; SANG KETUT SUDIRGA
SIMBIOSIS Vol I, No 2, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

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The black orchid (Coelogyne pandurata Lindl.) is an epiphytic orchid spesies that iscurrently endangered. To protect and maintain the genetic resources it is necessary to find theproper way to store seeds in a long time. The purpose this study is to determine the viability ofthe black orchid seeds sowed in organic medium with different storage period. The result showedthat the seeds sown in fresh condition has the highest percentage of growth up to 60%, seedswith storage period of 2 weeks has growth percentage of 20%, while the storage period of 4weeks has growth percentage of 2.5%. The growth phase of sowed seeds in fresh condition andseeds storage in 2 weeks periode is phase 4 (second leaf formed embryo) and seeds storage in 2weeks periode is phase 2 (green embryo). This suggest that the storage condition of black orchidseeds is influence physiology and seed viability.Keywords: Coelogyne pandurata Lindl., seed viability, storage periode

THE POTENTIAL OF CHITINASE ENZYMES PRODUCED BY CHITINOLYTIC BACTERIA ISOLATED FROM SHRIMP SHELLS AS BIOCONTROL CANDIDATES IN CONTROLLING PLANT PESTS Faldy Efriant Pangemanan; Ida Bagus Gede Darmayasa; Joko Wiryatno
SIMBIOSIS Vol 8 No 1 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Plant pests is one of the problem in agriculture sector that can cause decreasing of harvest productivity. Plant pest invasion will be increased in rainy season, so it need to be controlled. Biological control by chitinolytic bacteria is one of many ways to avoid the using of chemical pesticide. The purpose of this research to find the type of chitinolytic bacteria and utilizing the enzyme chitinase as plant pest biocontrol. Test has been done with spray method of 5 mL with 10%, 20%. 30%, 40% and 50% concentration with 7 treatments include positive and negative controls and 3 repetition. Observation of larva death done for 24 hours after spray, where every 6 hours observations are made. Pseudomonas is a genus that has been identified as chitinolytic bacteria. Larva death is indicated by with percentage result insignificant death which is shown at the 10 – 30% concentration. The percentage result significant death which is shown at the 40 – 50% concentration. 40% and 50% is most effective concentration in killing fruit fly (Drosophila sp.).

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Abstract

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Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota denpasar,

Bali

INDONESIA