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ELIMINATION OF Escherichia coli O157:H7 AND Escherichia coli O157 ON MEAT BY ULTRAVIOLET IRRADIATION Revi Juniar Nutrihidayah; Retno Kawuri; Inna Narayani
SIMBIOSIS Vol 9 No 1 (2021)
Publisher : Jurusan Biologi Universitas Udayana

Infection is a major problem mortality and morbidity in the world, a lot of an infection in the digestive tract is causes bacteria. The Escherichia coli O157 and Escherichia coli O157: H7 strains are bacteria that cause bloody diarrhea to death. Ultraviolet light has the potential to eliminate bacteria. The purpose is to know decontamination of E. coli O157 and E. coli O157: H7 on meat after ultraviolet exposure. The method is a factorial RBD with two factors (distance and time) with variations in distances (5, 10, 15, and 20 cm) and times (5, 10, 15, 20 dan 25 minutes). All treatments were repeated twice and obtained 160 samples. The quantitative data obtained were using univariant analysis followed by the Least Significant Difference with a significant level of 5%. The irradiation for E. coli O157 on meat with 5 cm for 25 minutes showed a significant reduction (88,62%), while the farthest distance 20 cm for 25 minutes decreased (73,42%). The nearest irradiation E. coli O157: H7 on meat 5 cm for 25 minutes show decrease (86,78%), while the farthest distance was 20 cm for 25 minutes (75,16%), there was an increase colonies in several treatment variations but overall decreased. In conclusion there was significant decrease number of bacterial colonies in meat given E. coli O157 culture, while there was a fluctuation decrease number of colonies in meats given E. coli O157: H7 culture.

EFEK SEDUHAN DAUN KELOR TERHADAP PERTUMBUHAN DAN PERKEMBANGAN ANAK TIKUS PUTIH JANTAN Ida Bagus Made Suaskara; Martin Joni; Putu Ariwati; Putu Agus Sumardika; Arofi Gusman Maulana
SIMBIOSIS Vol 4 No 2 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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ABSTRCT Determine food intake growth as it relates to the energy content in food. Protein plays an important role in the process of body growth, production and reproduction. This study aims to determine the weight gain, the number of red blood cells (tbsp), and the weight of testis and epididymis as criteria for the growth and development of the pups after by steeping the leaves of Moringa. This study uses a randomized complete block design (RAK) and divided into 4 groups and each group consisted of 10 pups male rats, namely; the test group 0 (K0 / control)) given aquadem, the test group 1 (K1) is given steeping the leaves of Moringa with a dose of 50mg / kg, the test group 2 (K2) is given steeping the leaves of Moringa with a dose of 75 mg / kg, the test group 3 ( K3) Moringa leaves steeping given at a dose of 100 mg / kg. Data results of the weight, the number of red blood cells, and weight of testis and epididymis were analyzed by statistical LSD. The results of this study showed steeping the leaves of Moringa provided can improve the weight and the number of red blood cells (tbsp) mice, whereas the weight of testis and epididymis are not increased significantly p = 0.550.Keywords: moringa leaves, dose, weight, number of tablespoons, testis and epididymis

DISTRIBUTION OF BALI STARLING (Leucopsar rothschildi) IN NUSA PENIDA ISLAND FX. Sudaryanto; Jusup Subagja; Satyawan Pudyatmoko; Cut Sugandawaty Djohan
SIMBIOSIS Vol 6 No 2 (2018)
Publisher : Jurusan Biologi Universitas Udayana

Bali Starling (Leucopsar rothschildi Stresemann, 1912) is endemic bird to the Bali Island. Since 1966, Bali Starling has been categoried as Critically Endangered by the IUCN Red List of Threatened Species, and the Indonesian Government Regulation No. 7 of 1999 on Preservation of Fauna and Flora. Conservation of Bali Starling has been done in the Bali Barat National Park, but has not succeeded yet. Therefore, starting in 2006 the conservation of the Bali Starling is also done in the Nusa Penida Islands, Klungkung Regency. To examine the successful of conservation of Bali Starling in Nusa Penida Islands, need the studies as follows: How is the distribution of Bali Starling? This research aims to study the success of conservation of Bali Starling in Nusa Penida Island. Specifically, the purpose of this research were to study distribution of Bali Starling. Materials and methods used in this research was known from questioned to the people in the area, and also conducted exploration. Distribution of Bali Starling in 2006 was only in three locations, and being expanded in 2015 there were at least in 12 locations. Keywords: Bali Starling, Leucopsar rothschildi, Nusa Penida Island, Bali,distribution

KEBERADAAN JENIS BURUNG DI PERSAWAHAN SUBAK LATU, SALAH SATU DAYA TARIK EKOWISATA Ida Bagus Made Suaskara
SIMBIOSIS Vol 4, No 1 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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This study aims to determine the existence of a type of bird species as one of the charms ekowista "bird watching" inthe paddy fields Subak Latu, Abiansemal, Badung. Methodology used in this research is the method of roaming acrosslocations conducted in September and October 2015. The time observations performed in the morning (06:00 to 9:30 pm),afternoon (03:30 to 6:30 pm). The observation there are 33 types of Berung identified where six species of birds, includingprotected (L) by government regulation because it is rare and appendix, namely; brown-throated sunbird (Anthreptesmalacensis), birds honey yellow (Nectarinia jugular), cekakak Java (Halcyon cyanoventris), cekaka river (Tordirhamphuschloris), herons buffalo (Bubulcus ibis) and blekok paddy (Ardeola speciosa) and one bird species that are not protected (TL)but where is rare in Bali as well as 26 types of commonly found. Utilization of the bird as the ecotourism attraction "birdwatching" in this area is the diversity of bird species, the status of birds, bird activity and bird interactions with vegetation /habitat. Activities of interest for eco-tourism attraction is the activity of foraging, flying move, perches and nesting.Keywords: ecotourism, bird watching, bird species, activity

PROFIL STRUKTUR SERAT IBU TANGKAI DAUN ANTARA INDUK DAN ANAKAN KELAPA (Cocos nucifera L “Rangda”) Ni Made Puspawati; Eniek Kriswiyanti; I Ketut Junitha
SIMBIOSIS Vol I, No 2, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

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The research aims to determine the anatomical structure of the leaf stalk fiber Coconut(Cocos nucifera L. "Rangda") on the seedling and parent plant. Sampling was conducted atSanghyang village Melaya district, Regency of Jembrana. Preparations and microscopicobservations made in the laboratory of Plant Development Structure Biology Department,FMIPA Unud. The research was conducted in October 2012 - April 2013. Maceration methodused for preparations sklerenkim fibers and xylem elements. The results showed that theanatomical parent plant and seedling petiole were torque and mostly composed bysclerenchym fibers but in the parent plant more torque than seedling. In the parent plant about81% the sclerenchym cell was torque but in seedling only 13%, with an average number of17 ± 1 torsion of the parent plant and 6 ± 1 in seedling.Keywords: the fiber structure, torque

KEANEKARAGAMAN JENIS CAPUNG DAN PEMANFAATAN NIMFANYA SEBAGAI NILAI TAMBAH PENDAPATAN DI BENDUNGAN LATU ABIANSEMAL Ida Bagus Suaskara; Martin Joni
SIMBIOSIS Vol 8 No 1 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Capung merupakan serangga yang berperan penting dalam keseimbangan ekosistem, berperan sebagai bioindikator dan predator. Sebagian besar fase hidupnya berada di air dalam bentuk larva. Beberapa jenis capung menempati tipe habitat perairan yang spesifik. Penelitian ini bertujuan untuk mengetahui keanekaragaman jenis capung dan pemanfaatan nimfanya sebagai nilai tambah pendapatan di persawahan Bendungan Latu Abiansemal . Metode penelitian yang digunakan adalah metode deskriptif dengan cara koleksi langsung menggunakan jaring serangga (insect net) untuk diidentifikasi. Hasil penelitian menunjukkan bahwa jumlah spesies capung yang ditemukan di sekitar Bendungan Latu sebanyak 7 spesies termasuk kedalam 2 famili dengan kemelimpahan relatif Orthetrum Sabina (47%), Pantala flavescens(44%), Neurothermis terminate (4%), Agriocnemis femina (2%), Neurothemis ramburii, Orthetrum chrysis dan Aethriamanta brevipennis masing masing 1%. Pola penyebaran capung 4 spesies mengelompok dan 3 spesies acak. Indeks keanekaragaman capung sangat rendah (H=1,04) dan pemanfaatan nimfa capung oleh petani digunakan sebagai lauk pauk untuk dijual serta penjualan nimfa capung hidup untuk pemenuhan kebutuhan taman capung di Desa Kemenuh Kabupaten Gianyar. Keywords: bioindikator, Orthetrum , Pantala, Neurothemis, Agriocnemis

UJI AKTIFITAS ANTIBAKTERI EKSTRAK ETANOL KULIT BUAH MANGGIS (Gracinia mangostana L.) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli Putri Permata; Retno Kawuri; AA Ketut Darmadi
SIMBIOSIS Vol 6 No 1 (2018)
Publisher : Jurusan Biologi Universitas Udayana

Mangosteen rind has a benefical compounds liked xanthone which consisting of mangostin, mangosterol, mangostinon A and B, trapezifolixanthone, tovophyllin B, alpha and beta mangostin, garcinon B, mangostanol, gartanin, and flavonoid epikatekin that known contain an antibacterial and antidiare agent. Escherichia coliis an opportunistic bacterium that is commonly found in the human colon as a normal flora. Its unique because it can cause primary infection in the intestine such as diarrhea in children, as well as its ability to cause infection in other body tissues outside the intestine.This research was done from December 2016 to March 2017. The methods that used in this research were well diffusion test to know the inhibitory power by measuring the clear zone formed. measure the clear zone of mangosteen rind against Escherichia coli. Then to testing extract compounds, there was used phytochemicals screening test. Data there has been found will be analyzed through analysis of variance (ANOVA). In the inhibitory test of ethanol extract from mangosteen rind (G. mangostana L.), it was so effective to inhibit the growth of Escherichia coli through in-vitro test with a resistivity of 1,58 cm and categorized as a strong inhibitory, which MIC from mangosteen rind against Escherichia coli was 3,9% and the compounds that contain in ethanol extract from mangosteen rind (G. mangostana L.) were alkaloid, phenolik, flavonoid, saponin and terpenoid. Key word : antibacterial activity, Garcinia mangostana L., Escherichia coli.

EFEKTIVITAS EKSTRAK DAUN SUKUN (Artocarpus communis Forst.) DALAM MENURUNKAN KADAR GLUKOSA DARAH DAN MEMPERTAHANKAN JUMLAH SPERMA PADA TIKUS (Rattus norvegicus L.) I Putu Ari Wijana Dipa; Ni Wayan Sudatri; Ngurah Intan Wiratmini
SIMBIOSIS Vol 3 No 2 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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Breadfruit (Artocarpus communis Forst.) leaves have a high antioxidant content. This research aims to know the effect of breadfruit leaf extracts on blood sugar levels and the amount of sperm in rat. This study used a Completely Randomized Design (CRD) using 30 rats aged 2-3 months and weighing 150-200 grams. Male rats were divided into six groups, i.e. negative control group (K1) without treatment, groups K2, P1, P2, P3 and P4 are given then NaCl 0.9%, breadfruit leaf extracts 50 mg/kg, 100 mg/kg, 200 mg/kg body weight, and glibenclamide 1 mg/kg body weight respectively. The treatments were given every day for 21 days. Blood sugar levels were measured before and after aloksan administration, day 7, 14, and 21 after treatment as well as counting the number of sperm after 21 days. The data obtained were analyzed with ANOVA and Duncan Multiple Range Test (DMRT), using the program of SPSS For Windows version 22. Results of the study showed that breadfruit (Artocarpus communis Forst.) leaf extracts could lowered blood glucose levels and the most effective concentration was 100 mg/kg body weight of breadfruit leaf extracts given for 21 days with the effectiveness of 66,77%. Breadfruit leaf extracts did not inhibit spermatogenesis. So the amount of rat sperm did not different significantly among treatment. Keywords: blood sugar levels, breadfruit, rat, sperm count

STRUKTUR HISTOLOGI HATI MENCIT(Mus musculus L.) SETELAH PEMBERIAN EKSTRAK DAUN EKOR NAGA (Rhapidhophora pinnata Schott) Ni Komang Tia Pramesti; Ngurah Intan Wiratmini; Ni Putu AdrianiAstiti
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

ABSTRACT Rhapidhophora pinnata, Schott is a traditional medical plant that has been use remedy for treatment of hypertension, stroke. Aims of this research is to determine the effect of Rhapidhophora pinnata Schott leaf extract toward mouse (Mus musculus L) liverhistological structure. This research used completely randomized design with 28 female mice that were divide into 4 groups of P0 (control) received 0,9% NaCl, group P1, P2, and P3 received 50, 100 and 150 mg/kg bw respectively by oral administration. The treatment were given daily for 14 days. All of the mice were sacrificed by ether after 15 days. The liver were examined for their histopathological changes, namely fatty degeneration, hydropic degenaration and necrosis. Results were statistically analised by Kruskal-Wallis method. Treatment groups showed no significant differences with regard of liver histopatological changes, however hemorrhage, sinusoid congestion and inflamatory cell infiltration were found in liver. Keyword : Rhapidhophora pinnata Schott, liver, mice

SPECIES OF CAPUNG (ORDER ODONATA) IN RICE PLANTS IN SEVERAL SAWAH SEKITAR DENPASAR, BALI Ni Made Suartini; Ni Wayan Sudatri
SIMBIOSIS Vol 7 No 1 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Dragonfly is an insect which belongs to the Odonata order and consists of the suborder Anisoptera (dragonflies) and the suborder Zygoptera (damselflies). Its habitat is very wide including in the rice field. Dragonflies and damselflies are predatory insects, both in the form of nymphs and adults, so their role is very important in maintaining the balance of the ecosystem. The research was conducted to finding out the dragonfly species found in rice plantations in rice fields around the Denpasar area. Sampling was done by catching using insect nets on rice plantations, in the morning from 08.00 to 11.00 WITA, and in the afternoon from 15.00 to 17.00 WITA. Dragonfly samples obtained were then preserved to be identified by observing the morphological characters based on Hanum et al. (2013), and Sigit et al. (2013). Dragonfly species found in rice plantations areas in several rice fields around Denpasar are as many as 8 species which are included in 2 suborder, namely suborder Anisoptera (5 species) and suborder Zygoptera (3 species), Species of suborder Anisoptera (Potamarcha congener) only found in rice plantations in East Denpasar. Keywords: insects, dragonfly habitat, identification, morphological characters

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Contact Name
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Journal Mail Official
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Location
Kota denpasar,

Bali

INDONESIA