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FLORAL DIVERSITY IN MINCIDAN VILLAGE, KLUNGKUNG, BALI TO SUPPORT ECOTOURISM Made Ria Defiani; Eniek Kriswiyanti
SIMBIOSIS Vol 7 No 1 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Flora diversity is one of natural resources for living of organism. The study aimed to explore type and usage of plants for medicinal at planned tracking area Mincidan Village to give benefit for education and ecotourism purposes. Method was descriptive analysis by surveying and giving some question to people around the village for plant conservation. Plants that were grown along the tracking site can be grouped as medicinal, ornamental, traditional uses on offerings. Based on the results, the plant can be identified 102 species, 71 genera in 38 families. The plants can be used for material on offering and traditional medicine.

JENIS LEBAH TRIGONA (APIDAE: MELIPONINAE) PADA KETINGGIAN TEMPAT BERBEDA DI BALI Niko Susanto Putra; Ni Luh Watiniasih; Made Made Suartini
SIMBIOSIS Vol 4, No 1 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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The aim of this study was to determine the of trigona species (stingless bee) live in different altitude in Bali. Samples were collected using purposive sampling technique, following earlier field observations to determine the location of the colonies. Samples were collected from three different locations, those were at Mawang and Taro Village at Gianyar Regency with the altitude of 750,87 m asl. Tua Village in Tabanan Regency with the altitude of 493,007 m asl, and Ngis Village in Karangase Regency with the altitude of 166,18 m asl, Species identifications were based on the morphological characters of nest entrance, the coloration of the body, wing venations, and the structure of bristle of hind tibia. Species identification were referred to Michener (2007), Schwarz (1939), Sakagami (1979), Sakagami and Inoue (1985), and Rassmusen and Cameron (2010). The result shows that out of 13 colonies collected from different altitude, ony 1 species was identified that was Trigona laeviceps. Keywords: Altitude, nest entrance, morphology, Trigona laeviceps

UJI FITOKIMIA DAN DAYA HAMBAT EKSTRAK DAUN JUWET (Syzygium cumini) TERHADAP PERTUMBUHAN Escherichia coli DAN Staphylococcus aureus ATCC Kadek Sudarmi; Ida Bagus Gede Darmayasa; I Ketut Muksin
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

Bacterial Escherichia coli dan Staphylococcus aureus ATCCis a normal flora bacteria that naturally exist human body. This bacteria may by pathogenic if it exceeds acertain limits. To prevent the disease caused, need to be overcome by using herbal ingredients one of them is juwet leaves (Syzygiumcumini).The purpose of the study was to determine wheter the extract of juwet leaf (S. cumini) can inhibited the growth of E. coli and S. aureus ATCC and to know compounds contained in juwet leaf extract (S. cumini). This research used diffusion wells methodwith 6 treatments and 4 replications. Date analysis using completely randomized design (CRD) with using ANOVA variance analysis. If the date obtained has a real difference test level at the 5% (P? 0,5) that continue with Duncan test. Concentration of the extract tested was 0%, 5%, 10%, 15%, 25% and 50%. Juwet leaf extract is able to inhibited the growth of E. coli and S. aureus ATCC which is shown by the formation of clear zone. Concentration extract leaf which is effective to inhibit E. coli and S. aureus ATCC is the concentration 50% (18,9 mm) for E. coli and (16,5 mm) for S. aureus ATCC. Screening of phytochemical test of juwet leaf extract positive containing alkaloids, phenolic, steroids and saponins. Keywords : juwet leaf extract (Syzygium cumini), Escherichia coli, Staphylococcus aureus ATCC

IN SILICO DNA PROBE DESIGN FOR MUTATION DETECTION OF QUINOLON RESISTANCE AREA gyrA AND gyrB GENE Mycobacterium tuberculosis Tasya Pramiswari; Jennifer Tamara; Ni Made Febrianti; Sagung Chandra Yowani
SIMBIOSIS Vol 7 No 2 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Fluoroquinolone (FQ) is the main drug used in MDR-TB therapy resistance to FQ can cause death and increase the risk of treatment failure in MDR-TB patients. Mutations in gyrA gene and gyrB gene from Mycobacterium tuberculosis are responsible for the occurrence of FQ resistance. The highest mutation of gyrA gene in QRDR was found in codon 94, while mutations in gyrB gene was found in codon 500. M. Tuberculosis which resistant to FQ can be detected using the Real Time Polymerase Chain Reaction (RT-PCR) method with DNA probe. This study will design the nucleotide sequence of the TaqMan type probe using the Clone Manager Suite 9.2 program. The results of the DNA probe design were then analyzed in two stages, which is based on the probe criteria in general and based on the TaqMan probe labeling criteria. The design of the mutant probe DNA using the program produced 1 probe for Asp94Ala specific mutations in the gyrA gene and 33 probes for Asp500Ala specific mutations in the gyrB gene. After being analyzed by the two criteria, it was obtained the A94MA1 probe with the 5 '-TCGATCTACGCCAGCCTGGT-3' sequence and B500MA12 probe with the order of 5 '-TACCACAAGCTCGTGCTGATGGC-3'. The results of these probes meet both criteria and can be used to detect mutations in codon 94 gyrA genes and codons 500 gyrB genes of Mycobacterium tuberculosis.

REDUCINGSUGAR CONTENTAND VITAMINE C IN BANANA (Musa paradisiaca forma typica)AFTER CURING BY ETHREL AND PLANT LEAVES Ni Putu Harta Diani Pande; Made Ria Defiani; Ni Luh Arpiwi
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

longer than other bananas and the availability is limited. One way to accelerate the availability of banana is by treating it naturaly andchemical. How to do in determining the ripeness of bananas as morphological and chemical analysis.The objectives of this study wereto explore the utilization of Gliricidia sepium leaf, cocoa leaf, banana leaf and ethylene and the duration of treatment to the maturitylevel of Musa paradisiaca forma typica, vitamin C, vitamin A, reducing sugar content as well as ripening time and rotting. CompletelyRandomized Design was used with 2 factors, the duration of curing with 3 treatments ie L1 (curing for 3 days), L2 (curing for 6 days)and L3 (curing for 9 days) and type of calibration method used was C0 (control), C1 (curing with ethylene 0,5%), C2 (curing withbanana leaf), C3 (curing with Gliricidia sepium leaf) and C4 (curing with cocoa leaf) . Each treatment combination was repeated 4times with 3 experimental units. The results showed that curing with cocoa leaves and 0.5% ethylene affected banana maturity.Ethylene (0.5%) increase the maturation score and vitamin C. Cocoa increased maturation score, reducing sugar and vitamin Ccontent. The longer the curing the more increase the maturation score and increase the reducing sugar content.Keywords: Musa paradisiaca forma typica, ethylene, cocoa leaf, banana leaf, G. sepium leaf

HUBUNGAN KEKERABATAN 12 KULTIVAR BROKOLI (Brassica oleracea L.) BERDASARKAN KARAKTER ANATOMI STOMATA Ni Putu Sri Risa Dewi; Eniek Kriswiyanti; I Gusti Ayu Sugi Wahyuni
SIMBIOSIS Vol 3 No 1 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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This research aims to know the relationship of kinship in 12 cultivars Broccoli grownin Horticultural Nursery at Kembang Merta village Bedugul Bali. Broccoli leaf samples weretaken from Horticultural Nursery at Kembang Merta village Bedugul Bali. The method usedin this research is by utilizing fresh preparations of upside/downside leaf epidermal incisionor Leaf Clearing method, the incision of the preparations and also the observations werecarried out at the Laboratory of Plant Structure and Development Department of Biology,Faculty of Science and Mathematics, Udayana University. The results showed the existenceof variations in epidermal cells, stomata structure, density and density of stomata on the 12cultivars of Broccoli (Brassica oleracea L.) with the range index of similarity between68,79% - 92,18% and formed the five groups that have different similarity indices in eachgroup.Keywords: anatomy, broccoli (Brassica oleracea L.), similarity index of stomata

KERUSAKANKROMOSOM BAWANG MERAH (Allium cepaL.) AKIBAT PERENDAMAN DENGAN ETIDIUM BROMIDA Eka Fibayani Imaniar; Made Pharmawati
SIMBIOSIS Vol 2 No 2 (2014)
Publisher : Jurusan Biologi Universitas Udayana

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The aim of this research was to identify thedamage of onion’s(AlliumcepaL.) chromosomes causedbyethidiumbromide submersion for 6and12hoursat 500ppm. The methodused to study chromosome damage of onionroot tip wassquash technique. The result showed several types of chromosomedamagesuch as the formation of, micronuclei, nuclear buds and chromosome bridges. At 6 hours submersion,the average percentageof chromosomal damage was 2.99 %, while in submersion for 12 hours, the average percentage of chromosomal damage was 6.81 %. Keywords:Ethidium bromide, chromosome damage, Allium cepa L.

USE OF MEDIA TYPES AND FERTILIZER TREATMENTS FOR THE GROWTH OF DENDROBIUM ORCHIDS Ida Ayu Meva Lianawati; Ni Putu Adriani Astiti; Ni Luh Suriani
SIMBIOSIS Vol 9 No 1 (2021)
Publisher : Jurusan Biologi Universitas Udayana

ABSTACT Reseach on dendrobium orchids was planted in the media of wood charcoal and coconut fibers and it is needed with Gandasil B fertilizer wood charcoal and coconut fiber media and is needed with Gandasil B and Multitonik fertilizer. This study aims to determine the effect of media and fertilizer on orchid growth and to determine pests and diseases in Dendrobium orchid plants. This reseach was conducted in May until July 2019 in the Plant Physiology Laboratory Biologi Prodi Studi Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana. Qualitative and quantitative data are used that combine the Gandasil B fertilizer and fibers and wood charcoal media Fertilizer Gandasil B fertilizer with concetration G0 (0 g L-1), G2 (20 g L-1), G3 (30 g L-1), G4 (40 g L-1), G5 (50 g L-1). Fertilizer Multitonik M0 (0 g L-1), M1 (1,1 g L-1), M2 (1,2 g L-1), M3 (1,3 g L-1), M4 (1,4 g L-1), M5 (1,5 g L-1). Each fertilizer concentration was repeated 6 times. The results showed the best orchid growth was found in the use of Multitonik fertilizer and the best orchid growth was found in the use of Multitonik number of leavers and root growth were found in the use of coconut fibers media

ANALISIS KEKERABATAN BEBERAPA TANAMAN MANGGA (Mangifera spp.) BERDASARKAN KARAKTERISTIK MORFOLOGI DAN ANATOMI DAUN Luh Putu Melandani; Eniek Kriswiyanti; Made Ria Defiani
SIMBIOSIS Vol 5 No 1 (2017)
Publisher : Jurusan Biologi Universitas Udayana

ABSTRACT The study was aimed to explore the characteristics of morphological, anatomical, and phylogenetic relationship between some types of mango (Mangifera sp.). Research conducted in Structures Laboratory of Plant Development Department of Biological Science, University of Udayana from April 2015 - July 2015. Samples were taken from three districts in Bali, that Denpasar, Gianyar and Badung. Ten species of plants of mango (Mangifera indica : ‘poh arum manis’, ‘poh bukit’, ‘poh gedang’, ‘poh madu’, ‘poh apel’,‘poh golek’, ‘poh sambuk’, ‘poh bali’, ‘poh manalagi’) and Wani (Mangifera caesia.) shows the character of morphology and anatomy of leaves varies as well as the analysis of kinship showed ten types of mangoes grouped into two major groups based on the degree of similarity. Fenogram shows the level of similarity of the ten types of mangoes in Bali grouped (cluster) into two: group I ‘poh arum manis’, ‘poh bukit’, ‘poh gedang’, ‘poh bali’, ‘poh apel’, and group II, namely ‘poh madu’, ‘wani’, ‘poh golek’, ‘poh sambuk’, ‘pohmanalagi’ where two clusters have a degree of similarity ? 63.91%.

THE APPLICATION OF ISOLATE LACTIC ACID BACTERIA (LAB) Lactobacillus bulgaricus and Streptococcus thermophilus IN BODY SCRUB PRODUCTION Bayu Putri Handayani; Retno Kawuri; Ni Luh Suriani
SIMBIOSIS Vol 6 No 2 (2018)
Publisher : Jurusan Biologi Universitas Udayana

The majority of body scrub that are distributed in the market contain paraben. Paraben is a type chemicals that are safe to use in the appropriate amount. But it could cause skin irritation. This research was conducted to know the durability from natural body scrub by yogurt and rice flour without chemical preservative and safe for skin. The research methods are comparison of the use of Lactobacillus bulgaricus and Streptococcus thermophilus with concentration 25:25; 30:20; 20:30. The yogurt will be mixed with rice flour and the treatment control using factory yogurt with concentration of rice flour comparing with yogurt namely 10:14; 10:16; 10:18. This product was tested to 50 panelists. This product was kept in room temperature ±28oC for a month and conducted the identification of microbial impurities that contaminate the product with Gram staining method and catalase test. Data was analyzed by analysis of variance (ANOVA). Starter with different comparison 25:25, 30:20; 20:30 are not making real difference. All of the treatment produce yogurt with typical aroma of yogurt, white colored, and soft texture. The formula in K2 (10:16) treatment with the highest average that obtained 3.38 (very like) in color parameter and 3.08 (very like) in texture parameter. Although in aroma parameter the average value that obtained is 2.79 (like). In A1, A2, and B2 treatment had the highest durability percentage that obtained 83,3% and the lowest was shown in K1 and K3 treatment that obtained 0%. Microbial impurities that identified was bacteria from Genus Bacillus and Genus Streptobacillus, fungal impurities from Genus Aspergillus, Rhizopus, and Trichoderma. Keywords : lactic Acid bacteria, yogurt, Lactobacillus bulgaricus, Streptococcus thermophilus, body scrub

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Journal Mail Official
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Location
Kota denpasar,

Bali

INDONESIA