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Brigitta Laksmi Paramita
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Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,193 Documents
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Sistematik Numerik Strain-Strain Anggota Genus Pseudomonas Pendegradasi Alkilbenzen Sulfonat Liniar Berdasarkan Sifat Fenotip dan Protein Fingerprinting Suharjono Suharjono; Langkah Sembiring; Jusup Subagja; Tri Ardyati; Lisa Lisdiana
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i1.2536

Abstract

Bacteria strains consisting of Pseudomonas sp. strain J and R isolated from river ecosystem polluted and Pseudomonas sp. strain A and B isolated from river ecosystem unpolluted by detergent were capable to degrade of LAS. The objective of this research was to determine similarity value by numerically of LAS-degrading Pseudomonas strains based on phenotype character and protein fingerprinting using three reference strains consist of Pseudomonas putida FNCC071, P. fluorescens FNCC070, and P. aeruginosa FNCC063. Phenotype characteristics examined are cellular and colony morphology, biochemical nature, capability to degrade polysaccharide, tolerance to various environmental factors and antibiotics, and ability to ferment sugar. Cellular protein fingerprinting was analyzed using SDS–PAGE discontinuous. Strains classification was determined based on Simple Matching Method similarity index by UPGMA (Unweight Pair Group Method with Average) algorithm. Based on phenotype nature, all strains have similarity value 0.61; however, based on cellular protein fingerprinting, those strains have similarity value 0.52. All strains of LAS-degraded were including in the genus of Pseudomonas.
Seleksi Dan Identifikasi Lactobacillus Kandidat Probiotik Penurun Kolesterol Berdasarkan Analisis Sekuen 16s Rna Evi Triana; Novik Nurhidayat
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i1.2537

Abstract

High fat and low fiber dietary pattern results in raising of blood cholesterol level over the normal level, namely hypercholesterolemia. Hypercholesterolemia might cause coronary disease and stroke. Blood cholesterol is able to be decreased by probiotic supplement. Lactobacillus is one of the probiotics that were well known and taken advantages. However its role as cholesterol lowering agent was less known. Therefore, screening and identification of Lactobacillus isolates which were candidates of probiotic have been carried out. Isolates Mar 8, Lac 3 and 7 p have been selected as Lactobacillus candidates for cholesterol lowering probiotic. Those isolates met criteria for cholesterol lowering probiotics. Furthermore, they have been conducted to confirm their identity as Lactobacillus. 16S RNA sequences analysis by BLAST analysis against reference strains within DNA Data Bank of Japan (DDBJ) have been carried on. Results showed that sequences of Lactobacillus Mar 8 was 100% homology with Lactobacillus plantarum, Lac 3 was 100% homology with Lactobacillus paracasei and 7 p was 99% homology with Lactobacillus plantarum. It was concluded that the three isolates were selected as candidates for cholesterol lowering probiotics. Both of them, Mar 8 and 7 p, are Lactobacillus plantarum. Another one, Lac 3 is Lactobacillus paracasei.
Eksplorasi dan Bioasai Berbagai Isolat Bacillus thuringiensis Lokal terhadap Larva Beberapa Jenis Serangga Dwi Suryanto; Chairani Chairani; Deddy Rusika; Nita A. Lubis; Yurnaliza Yurnaliza
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i1.2538

Abstract

Nine isolates of Bacillus thuringiensis of North Sumatra have been isolated from soil and dead larvae of Plutella xylostella. Bioassay of these isolates to larva of Heliothis armigera, Plutella xylostella, Aedes aegypti, and Culex sp. showed that the isolates have different spectrum and ability in controlling insect larvae. Interestingly, TU1 has similar ability to that of Dipel (B. thuringiensis var kurstaki strain HD-7) in controlling Dipteran (A. aegypti and Culex sp.) and Lepidopteran (H. armigera and P. xylostella). The others were not effective to Dipteran, but have various abilities to kill Lepidopteran. This result indicated that local isolates of B. thuringiensis of North Sumatra were varied in ability to kill different larvae.
Variasi Biokimia Genetik Populasi Ikan Betutu (Oxyeleotris marmorata, BLKr.) di Waduk Penjalin Brebes Agus Hery Susanto; Suhestri Suryaningsih
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2006): October 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.2539

Abstract

Penjalin water reservoir in Brebes Regency, Central Java, is one of the habitats of the sand goby. A study on its genetic diversity using approaches of isozyme analysis was needed to support domestication of the fish in this area. This study was aimed at the biochemical-genetic variation of sand goby population in Penjalin water reservoir based on esterase (EST), peroxidase (PER), malate dehydrogenase (MDH), aspartate amino-transferase (AAT), and acid phosphatase (ACP) polymorphisms. Visualization of the isozymes was carried out employing horizontal electrophoretic technique with potato starch gel and buffer system of L-histidin monohydrate. Of the five isozymes, ACP was not well-visualized in all samples tested while the remaining four showed no polymorphisms. It could be concluded that there is no biochemical-genetic variation of sand goby population in Penjalin water reservoir based on isozymes of EST, PER, MDH, and AAT.
Perbandingan Karakter Meristik dan Morfometrik Dua Jenis Ikan Lajur Kepala Kecil Eupleurogrammus muticus (Gray, 1831) and E. glossodon (Bleeker, 1860) (Percifomes: Trichiuridae) Andi Iqbal Burhanuddin; Yukio Iwatsuki
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2006): October 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.2540

Abstract

Penelitian ini bertujuan untuk membandingkan karakter meristik dan morfometrik antara dua jenis ikan lajur kepala kecil Eupleurogrammus muticus (Gray, 1831) dan E. glossodon (Bleeker, 1860) berdasarkan holotype, syntype dan non-type spesimen. Hasil penelitian ini menunjukkan bahwa kedua jenis ikan lajur tersebut dapat dibedakan berdasarkan karakter berturut-turut sebagai berikut: sirip dada pada jenis E. muticus terletak pada posisi sejajar dengan sirip punggung yang ke-17 dan 18 sedangkan pada E. glossodon terletak pada posisi yang ke-11 sampai 13; sirip dubur terletak pada posisi sejajar sirip pungung ke-40 sampai 42 (vs. 33-36); jumlah keseluruhan jari-jari sirip punggung sebanyak 142-148 buah (vs. 131-134); precaudal vertebrae 41-42 buah (vs. 32-36); jumlah total vertebrae 193-194 buah (vs. 160-163); kisaran panjang caudal peduncle 49% - 66% dari panjang tubuh depan dubur (vs. 29-37%). Karakter-karakter tersebut sangat penting untuk dijadikan sebagai dasar pembeda antara kedua jenis ikan lajur kepala kecil, E. muticus dan E. glossodon.
Studi Pakan Burung Perkici Pelangi (Trichoglossus haematodus Linnaeus, 1771) dalam Laboratorium Penangkaran W Widodo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2006): October 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.2541

Abstract

During the 2002-2003 period, the research was done to study 11 Rainbow Lorikeetsreared in an animal house laboratory. The aim of this research was to find the foodrations formule of the Rainbow Lorikeets so that those birds can be able to grow andbreed well. The food rations were composed of 26.3% local bird foods (521), 35.09%lampung bananas, 8.77% slice corns, 10.5% boiled quails eggs, 1.75% white bread,bean sprouts and red sugar are 8.77%, respectively. All of food materials were mixedon the plastic cup and mixed with 450 ml of water, then pulverized like sweetporridge. That porridge was given to birds in cafeteria and the water was made ready“ad libitum” everyday. The results have shown that giving food rations formula canstimulate two pairs of the Rainbow Lorikeets breeding and during the 2002-2003period they produced three young birds.
Kinetika Adsorpsi Ion Besi (II) Oleh Biomassa Chaetoceros sp. Paini Sri Widyawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2006): October 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.2542

Abstract

This research was done to study the adsorption kinetic of ferrous ionic by Chaetoceros sp. Many variables influenced the adsorption capacity of absorbent on ferrous ionic including interaction time, ferrous ionic concentration and pH. Two absorbens were used in this study i.e. pure culture and natural spoiled product called Diatomite/ Diatomae earth / Diatomaceous earth / Diatomooze of Chaetoceros sp. biomass. The adsorption capacity by biomass was measured by atomic absorption spectrophotometry method (AAS). The result showed that the adsorption process of two absorbents happened very fast. The time needed to get maximal adsorption were 10 and 15 minutes respectively. Adsorption pattern of two biomass can be interpreted by Langmuir and Freundlich isoterm showing monolayer. The adsorption capacity of Diatomite was five times higher than that of the pure culture biomass because it was influenced by surface group charge and wide surface area of porous. Acidity degree (pH) of solution determined surface active group charge and solubility of iron (II). The increased pH value, the biomass adsorption capacity was added because surface active group had negative charge. The adsorption of biomass was maximal around pH 5 while for pH higher than 5, it wasn’t significantly increased because the iron (II) formed insoluble hydroxide compound.
Sekresi Asam-asam Organik oleh Aspergillus niger YD 17 yang Ditumbuhkan dengan Batuan Fosfat Yudi Sastro; Donny Widianto; Dja'far Shiddieq
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2006): October 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.2544

Abstract

Information on ability of Aspergillus niger to secrete organic acid is important in using A. niger as phosphate rock-solubilizing microorganism. This research was aimed to examine the ability of A. niger YD 17 secreting organic acid when it was grown with phosphate rock. An A. niger YD 17 was obtained from Laboratory of Microbiology, Faculty of Agriculture, GMU. The phosphate rock used was Christmas Island phosphate rock. Organic materials consisted of tapioca waste industry, rice bran, and starch. The study was conducted in Pikovskaya liquid medium and soils that were taken from Jasinga, Banten, West Java (ultisol) and Karang Jati, Ungaran, Central Java (inceptisol). The type and level of organic acid production were determined using high performance liquid chromatography (HPLC). The results indicated that A. niger YD 17 was able to secrete organic acid when it was grown with phosphate rock. The level of organic acid in the Pikovskaya liquid medium reached 255.7 g.ml-1, whereas in the soil reached 2992.5 g.g-1. Malate dominated organic acid in the Pikovskaya liquid medium, while in the soils dominated by oxalate. The type and level of organic acid secreted by A. niger YD 17 were influenced by carbon and phosphorus sources, concentration of inoculums, and characteristic of the soils.
Skrining Tumbuhan Atraktif terhadap Lalat Buah Jantan Bactocera carambolae D&H Almahdy A; Deddi Prima Putra; Ahsol Hasyim; Mardinus Mardinus
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2006): October 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.2545

Abstract

Odour and frequently visited plants by Bactocera sp. were screened for fruit fly attractants. The attractiveness of hexane extracts for B. dorsalis were determined by Y-tube method. Of 20 species treated, 5 species such as Cymbopogon nardus, Ocimum basillicum, Cymbopogon citratus, Cananga odorata, and Hyptis suaveolens showed the attractiveness and the presence of methyl eugenol (ME). These plants could be used as the source of ME for controlling fruit flies infestation. It was also found that Ruta graveolens (Rutaceae) revealed the absence of ME peak by GC-spectrum but responded to fruit flies B. carambolae. The results reveal that the chemical compounds existing in the extracts of R. graveolens may have great potential for development as attractants of the Oriental fruit fly. However, further isolation, identification, and evaluation of active compounds are needed in future studies.
Karakterisasi Genetik Anjing Kintamani Menggunakan Petanda Mikrosatelit I Ketut Puja
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2006): October 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.2546

Abstract

Karakterisasi molekuler anjing Kintamani berperan penting dalam penentuan status trah dan mencegah penurunan kemurnian akibat perkawinan silang dengan trah anjing lain. Anjing Kintamani sangat popular di Bali. Anjing Kintamani dipercaya berasal dari Kintamani, Bangli, tetapi asal usulnya sampai kini belum jelas. Mikrosatelit merupakan runutan nukleotida terulang yang didistribusikan secara acak dalam gen vertebrata. Lokus mikrosatelit ini telah diketahui sangat polimorfik, karena keragaman jumlah nukleotida yang terulang. Mikrosatelit merupakan petanda allel utama yang dapat digunakan untuk pemetaan gen, genetika populasi dan identifikasi individu serta alat untuk mengungkap karakter genetik hewan. Pada penelitian ini diungkap karakteristik genetik anjing Kintamani menggunakan mikrosatelit. Jumlah dan ukuran allel pada 425 anjing Kintamani dianalisis menggunakan 116 macam primer mikrosatelit. DNA diisolasi dari sel hasil swab pipi. Amplifikasi 116 lokus mikrosatelit menggunakan PCR dalam 12 multiplek. Produk PCR dipisahkan dengan gel bis-akrilamid 6% dalam automated DNA sequencer. Flurosesnsi yang dihasilkan dideteksi dengan program Genescan 3.1, dan program Strand versi 2.2.39 digunakan untuk menghitung jumlah allel. Hasil penelitian menunjukkan bahwa jumlah allel yang didapat adalah 1128. Jumlah allel perlokus berkisar antara 3 (AHT136) sampai 41 (FH2138). Rataan PIC adalah 0,68 dan semua lokus bersifat polimorfik.

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