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JURNAL PEMULIAAN TANAMAN HUTAN
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Core Subject : Agriculture,
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Articles 249 Documents
KERAGAMAN GENETIK EMPAT POPULASI Arenga pinnata MERR BERDASARKAN PENANDA ISOZIM Liliek Haryjanto; Prastyono Prastyono; Burhan Ismail
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 1 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.1.13-21

Abstract

Aren tree or Sugar Palm (Arenga pinnata Merr) is one of bioethanol-producer which has advantages compared to other plant producing bioethanol. This study is  aimed to determine the genetic diversity of aren tree populations in its natural distribution. Genetic diversity of four populations were investigated by isozyme markers with 4 enzyme systems namely Esterase (EST), Glutamate oxaloacetate transaminase (GOT), Diaphorase (DIA) and 6-Phospogluconate dehydrogenase (6Pg). The number of alleles identified by 9 alleles are distributed on   four polymorphic loci. The research revealed that mean alleles per locus was 2,2500. The mean effective number of alleles per polymorphic locus was 1,8377. All loci were of  polymorphic. The mean genetic diversity within population was 0,4381and the proportion of genetic variation among populations was 0,0702. The UPGMA cluster analysis based on Nei’s standard genetic distance reflected two main clusters. The first cluster consisted of Central Java and South Kalimantan populations; while second cluster included the populations of Bengkulu and North Sulawesi.
UJI SOMATIK INKOMPATIBILITAS Ganoderma philippii UNTUK MENGETAHUI POLA SEBARAN PENYAKIT BUSUK AKAR PADA TANAMAN Acacia mangium Desy Puspitasari; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 4, No 1 (2010): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2010.4.1.49-61

Abstract

Ganoderma  philipii is a fungi causing root rot disease that have spread widely in Acacia mangium plantation. Study on mode of disease spreading is necessary to develop ways of controlling the spread of the disease. Somatic incompatibility test was carried out using fungal cultures that has been identified as G. philippii from two sites of different rotation. Each location showing different type of incompatibility. Compatible reaction was observed at Logas of first rotation indicating that the isolates are not genotypically distinct and may come from closely related clones. At this site the spread of root rot occurred by root to root contact. Incompatible reaction was observed at Deras of second rotation indicating that G. philipii isolate are genotypically distinct individuals and of different clones, eventhough they share same morphological characters. Genetic diversity found in G. philippii suggested that sexual reproduction of basidiocarps in an important factor and is strongly implicated as one of the main modes of dispersal as well as root to root contact.
EFEKTIFITAS FUNGI MIKORIZA ARBUSKULA DAN PLANT GROWTH PROMOTING BACTERIA TERHADAP PERTUMBUHAN Aquillaria crassna Ragil S.B Irianto
Jurnal Pemuliaan Tanaman Hutan Vol 9, No 3 (2015): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.031 KB) | DOI: 10.20886/jpth.2015.9.3.149-158

Abstract

Aquilaria crassna is an exotic plant producing agarwood. This research aimed to observe arbuscular mychorrhizal fungi (AMF) and plant growth promoting rhizobacteria (PGPR) effectiveness on growth of A. crassna in nursery and field. Experiments were arranged in a randomized completed blockdesign in the field. Seedlings were inoculated with Glomus ACA, Glomus SWM, PGPR, Glomous ACA+PGPR, and uninoculated seedlings as a control. Result showed that inoculation with Glomus ACA, Glomus SWM, dan PGPR could accelerated height and diameter of 8-months-old seedling significantly as much as 127, 128, 94; dan 51, 52, 39% compared to control. Inoculation Glomus ACA, Glomus SWM, still could be accelerated plant growth of height and diameter of 6-months-old young trees as much as 67.81% and 66.7% compared to control.
PENGUJIAN KARTU FTA SEBAGAI ALAT SAMPLING DNA JAMUR PATOGEN DARIBERBAGI BAGIAN TANAMAN YANG TERINFEKSI Purnamila Sulistyawati
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 2 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.2.93-104

Abstract

FTA  card offers a simple and fast method for retrieval of DNA samples at room temperature and storage of DNA in the short and long term. This will fasiliate the detection and identification of plant pathogens rapidly; increasing the number of samples can be collected, stored and transported in the field, especially from remote locations. The purpose of this study is to investigate  the suitability of FTA cards as a new method for sampling DNA from multiple infected of  plant tissues such as leaves, bark and roots. The results of this study shows that the FTA card suitable for use as one method of smpling because its quick and accurate. Series of PCR using ITSIF/ITS4 and ITS3/ITS4 primer pairs were done to detect the fungal DNA from the FTA card Nested PCR has been done using species – specific primers to identify the fungus without cloning. Optimization of methods to maximize the use of FTA cards as a method of sampling and DNA extraction which is simple, fast, accurate and safe for the environment is still needed. 
PENGUJIAN PENANDA JENIS SPESIFIK PADA JAMUR YANG BERPOTENSI SEBAGAI AGENS PENGENDALI HAYATI PENYAKIT BUSUK AKAR PADA AKASIA Istiana Prihatini; Anto Rimbawanto; Desy Puspitasari; Dayin Fauzi
Jurnal Pemuliaan Tanaman Hutan Vol 12, No 1 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.059 KB) | DOI: 10.20886/jpth.2018.12.1.1-11

Abstract

Root rot caused by Ganoderma philippii (Bres. & Henn. ex Sacc.) Bres., is an important disease in Acacia plantation. A strategy to control this disease is currently being developed, particularly on the application of biological control agent (BCA). Species specific primers for rapid identification of potential fungi as BCA were developed. This study aimed to obtain the best DNA condition and the best primers for species specific identification. DNA with 20x dilution is the best condition for amplification of the ITS fragment thus used for rapid species identification. The best primer set to detect Cerrena sp. is CrF1/CrR1, PbF2/PbR2 is the best primer set for Phlebiopsis sp. 1. There was no specific primer suitable to detect Phlebia sp. 1 and Phlebia sp. 2 only but Pl-2F1/Pl-2R4 is the best primer set for Phlebia spp.
FRAGMEN POLIMORFIK PENANDA RAPD UNTUK ANALISIS GENETIK SENGON (Falcataria moluccana) Vivi Yuskianti
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 1 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.1.55-61

Abstract

This research was conducted to support tree improvement program for sengon (Falcataria moluccana (Miq.) Barneby & J.W. Grimes). Polymorphic fragments were surveyed in 288 RAPD primers using 24 materials from Okinawa, Japan. Carefully selections of the polymorphic fragments were applied to overcome a problem of low reproducibility of RAPD markers. The first screening using 4 materials found that 285 out of 288 primers successfully produced fragments. The second screening using 16 materials finally obtained 48 polymorphic fragments. High discrimination ability (DP=0.483, in average) was obtained from all the selected fragments. The selected fragments can be used to support tree improvement program of the species for genetic diversity study and to facilitate a development of more specific markers such as Sequence Characterized Amplified Region (SCAR) marker.
KERAGAMAN GENETIK POPULASI Calophyllum inophyllum MENGGUNAKAN PENANDA RAPD (RANDOM AMPLIFICATION POLYMORPHISM DNA) ILG Nurtjahjaningsih; Titin Haryanti; AYPBC Widyatmoko; Sapto Indrioko; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 9, No 2 (2015): Jurnal pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.852 KB) | DOI: 10.20886/jpth.2015.9.2.103-115

Abstract

The aims of this study were to assess genetic diversity within populations and genetic relationship among populations of C. inophyllum. Leaf samples as template DNA were collected from 10 natural populations and 1 plantation. Five random amplified polymorphism DNA (RAPD) markers consisted 30 loci were conducted to genetic analysis. Results showed genetic diversity within populations were in low to moderate level (mean HE=0.186). There is no private allele in any populations. The analysis of molecular variance (AMOVA) showed that genetic differentiation among Islands was insignificant; but the differentiation was siginificant among populations and individual trees. Genetic distance among populations was in low to moderate level (mean Da=0.250). Cluster analysis clearly divided the 11 populations into 2 clusters; cluster I consisted Selayar, Lombok, Gunung Kidul and Padang populations; cluster II consisted Way Kambas, Madura, Ketapang, Dompu, and Yapen populations. The genetic relationships did not associate with their geographical locations. In conclusion, genetic diversity and genetic relationship among populations of C. inophyllum was in moderate level.
ANALISIS SISTEM PERKAWINAN MERBAU (Intsia bijuga O. Ktze) DI PAPUA BERDASARKAN PENANDA ISOENZIM Mahfudz Mahfudz; Mohammad Na'iem; Sumardi Sumardi; E B Hardiyanto
Jurnal Pemuliaan Tanaman Hutan Vol 4, No 2 (2010): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2010.4.2.109-116

Abstract

Merbau (Intsia bijuga O. Ktze) is an important species to supply wood in Indonesia. Mating system on natural population of merbau can influence genetic diversity of merbau. The objectives of this study were to investigate mating system on natural forest of merbau in Papua. The research used 200 samples of merbau from 10 mother trees in Gunung Meja Forest Research Manokwari-Papua. Isoenzyme analysis was done using four enzyme systems i. e. Peroxidase (POD), Esterase (EST), Glutamate Oxaloacetate Transaminase (GOT) dan Diaphorase (DIA). Data were analysed using MLTR program. The result showed that average genetic diversity (He) 0.463 and total heterozygosity (HT) 0.496. The multilocus population outcrossing rate (tm) and singlelocus population outcrossing rate (ts) were tm = 1.00 and ts = 0.992. Probability of inbreeding rate (tm  - ts) = 0.008.
PENINGKATAN KUALITAS BIBIT NYAMPLUNG (Calophyllum inophyllum L) DAN MALAPARI (Pongamia pinnata L) DENGAN APLIKASI MIKORIZA DAN Trichoderma spp. Benyamin Dendang; Aditya Hani; Encep Rachman
Jurnal Pemuliaan Tanaman Hutan Vol 12, No 1 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (453.269 KB) | DOI: 10.20886/jpth.2018.12.1.75-84

Abstract

Nyamplung and Malapari a potential bioenergy crops on marginal land to be developed on the coastal land. The constraints of the coastal land is the less of water and soil nutrient. Input technology is needed in order to suceed the crop planting such as using bio fertilizer. Mycorrhizae plays an essential role in helping plants to absorb phosphate and increase the resistance to drought. Trichoderma spp involves in producing beneficial hormones for metabolism and helps break down organic matter. Utilization of biofertilizer is still rarely used as treatment in bioenergy plant seedlings. This study aimed to determine the quality of nyamplung and malapari seedlings after mycorrhizal and Trichoderma spp application in the nursery. Experiments were arranged in a complete randomized block design (RCBD). The treatment of media: soil as control (T), soil+organic fertilizer with ratio of 3:1(TK), soil+organic fertilizer+mycorhizae 10 g (TKM), soil+organic fertilizer+Trichoderma spp 10 g (TKT), soil+organic fertilizer+mycorrhizal 10 g+Trichoderma 10 g (TC). Application was done when the seedlings reached 1 (one) month old. Biofertilizer was sown around seedling roots. Each treatment consisted of 50 samples and 3 replications so the total seedlings were 750 seedlings. The results showed that after 6 and 12 months treatment malapari with organic fertilizer + mikoriza + Tricoderma spp (TC) gained 67.5% taller and 427% larger on dry weight of leaf than the control, whereas in nyamplung seedlings, TC treatment resulted better growth with diameter 8% larger, root length 78.56% longer, and total dry weight 7.88% heavier compared to control (T).
FLOWERING AND FRUITING STUDY OF Melaleuca cajuputi subsp. cajuputi Powell AT PALIYAN SEEDLING SEED ORCHARD, GUNUNGKIDUL, YOGYAKARTA Liliana Baskorowati; R Umiyati; Noor Khomsah Kartikawati; Anto Rimbawanto; Mudji Susanto
Jurnal Pemuliaan Tanaman Hutan Vol 2, No 2 (2008): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2008.2.2.189-202

Abstract

Observation on of flowering and fruiting phenology  were undertaken at M. cajuputi subsp. Cajuputi seedling seed orchard Paliyan, Gunungkidul from February to October 2002. Seed viability was tested out at Forest Biotechnology and Tree Improvement Research Institute, Yogyakarta. The objectives of this study are: (1) to identify the flowering time, (2) to determine the time of buds development and (3) to observe the reproductive success and the number of germination seeds per capsule of open pollination seeds. The study showed that peak flowering of M. cajuputi subsp cajuputi at Paliyan seedling seed orchard occurred in March; several trees at this population also flowered during February to June. The buds initiation to capsules maturity took on average of 277 days. Three months after fertilisation, the capsules were ready to harvest. Mean number of reproductive success of open pollination seeds is 3.6%. The mean number of seed viability varied between provenance range from 6 to 32 seeds/capsule.

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