cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Isna Rasdianah Aziz
Contact Email
isna-rasdianah@uin-alauddin.ac.id
Phone
+6285242001984
Journal Mail Official
biogenesis@uin-alauddin.ac.id
Editorial Address
Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar. Jl. H.M. Yasin Limpo No. 36 Samata, Gowa, South Sulawesi, Indonesia. 92113
Location
Kab. gowa,
Sulawesi selatan
INDONESIA
Biogenesis: Jurnal Ilmiah Biologi
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23021616     EISSN : 25802909     DOI : https://doi.org/10.24252/bio
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic publication for Islamic scientists, which could contribute to the advancement of tropical biology studies in the Islamic world. The journal invites research articles, short communication, critical and comprehensive scientific reviews concerning tropical biosciences in Asia, specifically in the following subjects: Islamic-integrated tropical bioscience Tropical biodiversity and conservation Biosystematics of tropical species Biochemistry and physiology of tropical species Ecological study and behavior of tropical species Tropical genetics, biotechnology, and bioinformatics
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 14 Documents
 1   2 
Search results for , issue "Vol 12 No 2 (2024)" : 14 Documents clear
Antioxidant potential of ethanol, ethyl acetate, n-hexane extracts from leaves, fruits, stems, and roots of red okra (Abelmoschus esculentus) Putri, Salsabilla Wiyana; Hapsari, Lukiteswari Dyah Tri; Junairiah; Wahyuningsih, Sri Puji Astuti
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.43666

Abstract

Abelmoschus esculentus L. Moench has been known for its abundant and affordable antioxidant potential. This plant has different antioxidant levels regarding the parts of the plant. Red okra is rich in flavonoids and polyphenols, including strong antioxidant as quercetin ad anthocyanin. This research is intended to determine the IC50 value and compare the total content of phenolic and flavonoid from ethanol, ethyl acetate, and n-hexane extract of red okra (A. esculentus) leaves, fruits, stems, and roots. Extraction process was done by gradual maceration method using ethanol, ethyl acetate, and n-hexane. IC50 value interprets the antioxidant activity obtained from DPPH test. The results showed a very strong antioxidant activity from red okra stem extract with IC50 of 25.22 ppm and the lowest from fruit n-hexane extract with 821.55 ppm. From TPC test, the highest content was obtained from stem ethanol extract with 156.01 mg GAE/g and the lowest from stem n-hexane extract with 25.99 mg GAE/. From TFC test, the highest content was obtained from fruits ethanol extract with 108.15 mg QE/g and the lowest from stem ethanol extract with 2.26 mg QE/g. In conclusion, this research showed that red okra (A. esculentus) contains a high level of phenol and flavonoid compound and the highest antioxidant content was shown in ethanol extract.
Shoots induction of axillary buds in blueberries (Vaccinium corymbosum L.) in vitro with the addition of BAP and IBA Restanto, Didik Pudji; Budha, Al Sura Tri; Prayoga, Mohammad Candra; Slameto, Slameto; Khozin, Mohammad Nur
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.46312

Abstract

Blueberry Vaccinium corymbosum L. is a fruit plant that has high economic value and many benefits. Blueberries in Indonesia are not yet in great demand so domestic needs are still met by imports. Conventional blueberry propagation is done using stem cuttings. The disadvantages of conventional propagation are that seedling growth is slow, requires a large source of planting material, and is ineffective. The aim of the research was to determine the effect of the combination of BAP and IBA hormones on the induction of axillary shoots in effective in vitro propagation of blueberries. The explants used were axillary buds of blueberry, sterilized using fungicide, bactericide, bayclin, sodium hypochlorite and alcohol. The research design used a completely randomized design (CRD) with a combination of BAP and IBA hormones. The BAP hormone consists of three concentrations, namely 0.5, 1.0, and 1.5 mg/L. The IBA hormone consists of three concentrations, namely 0, 0.1, and 0.2 mg/L. Based on research results, the combination treatment of BAP and IBA succeeded in inducing shoots, but was not able to induce roots. The BAP 0.5 mg/L treatment showed the best results with a shoot emergence rate of 4.33 weeks, shoot height of 1.68 cm, number of shoots 1.67, and number of leaves 12.67. The findings of this study highlight the potential of BAP and IBA hormone combinations in accelerating blueberry shoot induction, offering an alternative propagation method that could support domestic production and reduce dependence on imports.
Potensi Senyawa Bioaktif Andrographolide Pada Daun Sambiloto (Andrographis paniculata) Sebagai Kandidat Anti Photo-Aging Secara In Silico Zuhriyah, Nur; Fatkhurrohman, Fajar; Aida, Monika; Apriliani, Sindi; Setiyono, Eko
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.57447

Abstract

Skin aging caused by UV radiation has become a growing concern among the Indonesian population. The effects of UV-induced skin aging can be mitigated by antioxidant compounds. Although synthetic antioxidants are commonly used to combat aging, their long-term use is discouraged due to potential systemic side effects and carcinogenic risks. Therefore, natural antioxidant compounds are needed as a safer alternative. Andrographis paniculata, known as Sambiloto, contains a natural antioxidant compound called andrographolide. This study aims to evaluate the potential of andrographolide by determining its binding affinity toward target protein receptors involved in the photoaging mechanism triggered by increased levels of Reactive Oxygen Species (ROS). Through in silico analysis, the potential of andrographolide was assessed using reverse docking techniques with databases such as PubChem, PharmMapper, SwissTargetPrediction, SuperPred, and PASS Online. Subsequent molecular docking was performed using PyRx 0.8 and PyMol software. The in silico data revealed interactions between the ligand compound and its receptors, including binding activity and binding positions based on hydrophobic interactions and hydrogen bonds, which were further analyzed. The results showed that andrographolide binds effectively to the IL-1, NF-κB, and IL-6 receptors with relatively low binding affinities of -7.5 (IL-1), -6.6 (NF-κB), and -6.5 (IL-6). Low binding affinity indicates stronger and more stable interactions between the ligand and receptor. Based on the results, andrographolide from A. paniculata exhibits promising potential as a candidate for anti-photoaging agents that can serve as an alternative to synthetic antioxidants.
Protective effect of juwet fruit extract (Syzygium cumini L.) on duodenum histomorphometry and histopathology of male mice exposed to pyrantel pamoate Susilowati, Rina Priastini; Santoso, Adit Widodo; Sabini, Jasmine Harumi
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.56552

Abstract

Pyrantel pamoate, known as an anthelmintic under the brand name Combantrin®, is effectively used to kill worms in the intestines. However, long-term use of it has been associated with side effects and risk of drug resistance. Syzygium cumini L.is known for its antioxidant and anti-inflammatory which may support antiparasitic effects and tissue healing. This study aims to examine the effect of juwet (S. cumini L.) fruit water extract (JFWE) on the duodenum structure of mice suspected to be infected with worm, through histomorphometric analysis and histopathological examination. The pyrantel pamoate was only administered once after the acclimatization period, while the JFWE was administered for 3 weeks. A total of 30 male mice were divided into six groups: negative control (aquadest), positive control (pyrantel pamoate, Combantrin® 20 mg/mL), and JFWE at doses of 5 mg/mL, 10 mg/mL, 20 mg/mL, and 40 mg/mL, administered for 3 weeks. Histomorphometric and histopathological evaluations were conducted by microscopically observing the duodenum structures, including intestinal villi, and the thickness of the mucosa, submucosa, and muscularis layers. The results showed a significant difference for the decrease in intestinal villi height and thickness of the mucosa, submucosa, and muscularis layers in JWFE doses of 5 mg/mL and 10 mg/mL administration group, compared to the negative control group (aquadest), the treatment groups of pyrantel pamoate, and JFWE doses of 20 mg/mL and 40 mg/mL (p<0.05). The conclusion is that the administration of juwet fruit water extract doses of 20 mg/mL and 40 mg/mL can improve the histomorphometry structure and also the histopathology of the duodenum of male mice.
Biofertilizers improve growth rate, nitrate reductase activity, and productivity of shallot (Allium cepa L.) under drought stress Lena Mardiana; Siswanti, Dwi Umi
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.57317

Abstract

Shallot (Allium cepa L.) is one of Indonesia's horticultural crops and represents as superior commodity. However, shallots are highly susceptible to drought and require sufficient water for growth. Biofertilizers contain various microbes that provide nutrients and increase the resistance of shallots to drought stress. This study aimed to determine the effect of biofertilizers on the growth rate, nitrate reductase activity, and productivity of shallot plants under drought stress. The research was conducted using a Factorial Completely Randomized Design with two factors. The first factor was the doses of biofertilizer with 0, 10, 15, and 20 L ha-1. The second factor was the drought stress with treatments of 25, 50, 75, and 100% field capacity. Parameters measured included plant height rate, leaf number rate, number of tillers, tuber wet weight, tuber dry weight, and nitrate reductase activity. Data were analyzed by ANOVA using SPSS followed Duncan’s Multiple Range Test (DMRT) at 5% significance level. The results showed that biofertilizer application did no significantly different on the growth rate, but significantly affected the number of tillers, tuber weight, and nitrate reductase activity of shallots under drought stress. The optimum dose of 10 L ha-1 biofertilizer increased the number of tillers (9.33). The optimum dose of 20 L ha-1 biofertilizer increased the growth rate, tuber wet weight (4.46 g), tuber dry weight (0.63 g), and nitrate reductase activity (1.11 µmol NO2- g-1 leaf wet weight h-1 of incubation). It is concluded that biofertilizer application, particularly at 10–20 L ha-1, can improve shallot performance under drought stress and is recommended as a drought mitigation strategy in shallot cultivation.
Sensitifitas Tahapan Spermatogenesis dan Perubahan Profil Stress Oksidatif Jaringan Testis akibat Paparan Toksikan dalam Lindi Yulianty, Nurfitri; Istanti, Edwina; Yusuf, Ayda Trisnawaty; Irawan, Afri
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.57633

Abstract

Exposure to environmental waste such as landfill leachate has the potential to cause genotoxic effects and oxidative stress impacting the mammalian reproductive system. This study examined the effects of Sarimukti Landfill Leachate (LTS) on sperm DNA damage and testicular oxidative stress in male Wistar rats. Rats were exposed to LTS at concentrations of 0%, 35%, and 63% during distinct spermatogenesis stages: mitosis (MTS), meiosis (MSS), and spermiogenesis (SMGS). Sperm DNA damage was assessed using the Sperm Chromatin Dispersion (SCD) assay, while testicular oxidative stress was measured by quantifying Malondialdehyde (MDA) and total protein levels. The results showed dose-dependent sperm DNA damage across all spermatogenesis stages, with the most significant damage observed at the 63% LTS dose. Sperm DNA damage levels were highest in the MTS group (93.53%), followed by MSS (87.5%) and SMGS (86.8%). The 63% LTS dose also increased testicular lipid peroxidation, as indicated by elevated MDA levels (4.63 µM/mL). This suggests that LTS can damage lipid components in testicular tissue. Additionally, the 63% LTS dose reduced total protein levels in testicular tissue to 63.55 µg/mL, compared to 108.31 µg/mL in the control group. Heavy metals (arsenic, plumbum, nickel, chromium) and organic pollutants in LTS generated reactive oxygen species (ROS) in germ cells, disrupting cellular repair mechanisms also inducing lipid and protein oxidation. This oxidative stress further exacerbated sperm chromatin fragmentation. These findings highlight the reproductive toxicity of landfill leachate and emphasize the need for protective measures during early spermatogenesis stages. This study provides insights into the environmental impacts on male fertility and underscores the risks of genotoxic exposure.
Staphylococcus aureus contamination in traditional Balinese Indonesian stem food: Tum bungkil, Tum pusuh and Tum biu batu Kusumaningsih, Purwaningtyas; Puspaningrum, Dylla Hanggaeni Dyah; Dachi, Milafebriani; Mboru, Adriana Dehi
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.55600

Abstract

Tum is a traditional Balinese Indonesian dish made from various parts of the stone banana tree, including the stone banana root bulb (bungkil), stone banana flower (pusuh), and stone banana fruit (biu batu). However, when tum is made by hand without gloves, there is a risk of contamination with Staphylococcus aureus. Although the steaming process may reduce some bacteria, contamination can still occur. S. aureus can survive in ready-to-eat foods prepared under poor hygiene conditions. The toxins produced by S. aureus can lead to skin infections, respiratory problems, and sepsis. This study aims to investigate the presence of S. aureus in processed tum. Purposive sampling was conducted in two districts that sell tum bungkil, pusuh, and biu batu. Six samples were tested using mannitol salt agar (MSA) which is specific media from a dilution level of 10-3. Identification is conducted based on morphology, gram staining and total plate count (TPC) results. The results indicated that the highest level of contamination was found in sample 3 (S3) were 1.7x102CFU/g, which contained tum pusuh. This study implies that it is essential to improve hygiene practices during the preparation of tum pusuh to minimize the risk of contamination.
Lipid from microalgae isolated from Cengklik Reservoir, Boyolali, Central Java for prospective biodiesel sources Sari, Siti Lusi Arum; Suratman, Suratman; Sunarto, Sunarto; Purwoko, Tjahjadi; Larasati, Dewi; Herina, Stefanus Junliem Nugratama
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.55910

Abstract

The availability and sustainability of the algae-based biodiesel industry depend on the adequate selection of microalgae strains. Based on this, it is important to explore local microalgae from Indonesia as a feedstock for biodiesel. This study aimed to isolate and screen freshwater microalgae with lipid-producing abilities. A total of three microalgal isolates have been successfully isolated from the Cengklik Reservoir (-7.506712, 110.723582). Based on the cell morphology, the three isolates were identified as Chlorella sp., Scenedesmus sp., and Oscillatoria sp. Nile red staining indicated that the three microalgal isolates contained neutral lipid droplets in their cells, as shown by the presence of a yellow or orange luminescence under a fluorescence microscope. The results of this study indicated that Scenedesmus sp. and Oscillatoria sp. had more potential as feedstocks for biofuels due to their higher lipid content and productivity. This research succeeded in obtaining microalgae isolates from Cengklik reservoir, Boyolali, Central Java which can be used as a source of lipids for biodiesel.
Genetic diversity analysis among Philodendron spp. by Random Amplified Polymorphic DNA Karmawan, Listya Utami; Gunawan, Renaldy
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.58061

Abstract

Variety development in ornamental plants increased rapidly due to the high demand during the COVID-19 outbreak. Therefore, analyzing the genetic diversity of ornamental plants is essential to assist breeders in improving and developing new cultivars with desirable traits. In this study, the genetic diversity of Philodendron species was analyzed. P. erubescens, P. 69686, P. burle-marx, P. lacerum, and P. micans were selected as the most popular species cultivated in Indonesian nurseries. These cultivars were assessed both genetically using Random Amplified Polymorphic DNA (RAPD) markers and morphologically. The genetic relationship among Philodendron spp. species was analyzed using DARwin 6.0 software, and a dendrogram was constructed based on the UPGMA algorithm. Based on the two selected RAPD primers, the five samples showed no clear genetic relatedness to each other, and the primers were insufficient to construct a single consensus dendrogram.
A comparative study of bacterial colony counts and Enterobacteriaceae isolates in pregnant women across pregnancy trimesters Suwanto, Yustiana Arie; Suryani , Annisa Saffana Azmi
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.58208

Abstract

Urinary tract infections (UTIs) are common during pregnancy and may lead to serious complications for both mother and foetus. Anatomical and hormonal changes throughout gestation, particularly bladder compression and urinary stasis, create favourable conditions for bacterial colonisation and subsequent infection. Escherichia coli and other Enterobacteriaceae are the predominant pathogens, and urine culture remains the definitive diagnostic method, with a threshold of ≥10⁵ CFU/mL indicating infection. The objective of this study is to investigate the prevalence and distribution of bacterial colony counts and the presence of Enterobacteriaceae in pregnant women across all trimesters, as well as to assess the relationship between gestational age, bacterial load, and the incidence of UTIs. This observational cross-sectional study involved 46 pregnant women at various gestational stages. Midstream random urine samples were collected and subjected to microbiological culture. The number of colony-forming units per millilitre (CFU/mL) and the species of bacteria isolated were recorded and analysed descriptively. UTIs was identified in 9 out of 46 participants (19.6%), characterised by bacterial counts ≥10⁵ CFU/mL. The trimester-specific prevalence showed that 11% of cases occurred in the first trimester, 33% in the second trimester, and 56% in the third trimester. The most frequently isolated pathogens were Escherichia coli (80%), Staphylococcus spp. (75%), Enterococcus spp. (25%), and Klebsiella pneumoniae (20%). UTIs were more prevalent in the later stages of pregnancy, with the third trimester showing the highest incidence. E. coli remained the leading uropathogen. These findings underscore the importance of early screening through urine culture to prevent adverse maternal and neonatal outcomes, particularly during the third trimester.

Page 1 of 2 | Total Record : 14

 1   2 

Filter by Year

2024 2024


Reset
Filter By Issues
All Issue Vol 13 No 1 (2025) Vol 12 No 2 (2024) Vol 12 No 1 (2024) Vol 11 No 2 (2023) Vol 11 No 1 (2023) Vol 10 No 2 (2022) Vol 10 No 1 (2022) Vol 9 No 2 (2021) Vol 9 No 1 (2021) Vol 8 No 2 (2020) Vol 8 No 1 (2020) Vol 7 No 2 (2019) Vol 7 No 1 (2019) Vol 6 No 2 (2018) Vol 6 No 1 (2018) Vol 5 No 2 (2017) Vol 5 No 1 (2017) Vol 4 No 2 (2016) Vol 4 No 1 (2016) Vol 3 No 2 (2015) Vol 3 No 1 (2015) Vol 3, No 1 (2015) Vol 2 No 2 (2014) Vol 2, No 2 (2014) Vol 2 No 1 (2014) Vol 2, No 1 (2014) Vol 1, No 2 (2013) Vol 1 No 2 (2013) Vol 1, No 1 (2013) Vol 1 No 1 (2013) More Issue