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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

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Morphometric performances of Katingan cattle in Central Kalimantan Utomo, Bambang Ngadji; Noor, R.R; Sumantri, C; Supriatna, I; Gunardi, E.D
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 3 (2010)
Publisher : Indonesian Animal Sciences Society

The information of Katingan cattle as an indigenous cattle in Central Kalimantan is very limited, especially phenotypic characterization. A number of 72 heads, consist of 57 heads female and 15 heads male of Katingan cattle in adults category were used in this study. The samples were obtained from three sub districts in district of Katingan, those were Tewah Sanggalang Garing (Pendahara Village), Pulau Malan (Buntut Bali Village) and Katingan Tengah (Tumbang Lahang Village). The location were used as the variable of groups (subpopulation). This study was conducted to describe morphometric performance, which was analysed by discriminant and canonical to estimate the phylogenic relationship and to determine the discriminant variable among the group (subpopulation). The SAS package program version 6.0 and MEGA 2 program was used to analyze data. The observed parameters were body length, wither height, chest width, chest depth, chest circumference, hip heigth, hip width, head height, head length and head width. The chest depth and hip height of female cattle from group of Buntut Bali were lower (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The parameter of: chest width, hip width and body length of Buntut Bali groups were higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The average body weight of male Katingan cattle from Buntut Bali was higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups but there were not different (P > 0.05) on bodyweight of female Katingan cattle among location. The highest similarity value was Katingan cattle from group of Tumbang Lahang (92,86%) followed by group of Pendahara (40.91%) and group of Buntut Bali (38.89%), repectivelly. Katingan cattle from group of Pendahara and Tumbang Lahang had closest genetic distance (1.406), while the longest genetic distance (2.046) was between group of Buntut Bali and group of Tumbang Lahang. Genetic distance between group of Buntut Bali and Pendahara was 1.929. Katingan cattle from Pendahara and Tumbang Lahang within one cluster. Body length in canonical structure 2 can be used as discriminant variable. Key Words: Katingan Cattle, Morphometric, Genetic Distance, Discriminant Analysis, Canonical

Genetic characteristic of protein membran of avian influenza viruses H5N1 subtype Dharmayanti, N.L.P Indi; Hewajuli, D.A; Ratnawati, A; Indriani, R; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 3 (2010)
Publisher : Indonesian Animal Sciences Society

In 2006-2008 there were findings about the antigenic drift on AI virus due to vaccination and the AI H5N1 subtype viruses which was similar to H5N1 viruses in human. The findings indicated that the AI viruses continue and undergoing to mutate and try to adapt with their environment.Â The objective of this study was to characterize the mutation of recent AI viruses (2009) on the membran protein namely Hemagglutinin (HA), Neuraminidase (NA) and Matrix 2 (M2). In this study RT-PCR â sequencing methods and genetic analysis for the protein membran of AI viruses were used. Result revealed that there were specific mutation belong to AI 2009 viruses on HA and NA protein such as AI virus mutation in 2008 which was isolated from backyard chicken. The mutations were non synonimous and not caused by immunological pressure. Furthermore, M2 analysis indicated that the viruses were resistant to amantadine. Key Words: Mutation, AI Subtype H5N1 Viruses, Membran Protein

Characterisation of the H5 and N1 genes of an Indonesian highly pathogenic Avian Influenza virus isolate by sequencing of multiple clone approach Hartawan, Risza; Robinson, Karl; Mahony, Timothy; Meers, Joanne
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 3 (2010)
Publisher : Indonesian Animal Sciences Society

Hemagglutinin and neuraminidase are the main antigenic determinants of highly pathogenic avian influenza (HPAI) virus. The features of these surface glycoproteins have been intensively studied at the molecular level. The objective of this research was to characterise the genes encoding these glycoproteins by sequencing of multiple clones. The H5 and N1 genes of isolate A/duck/Tangerang/Bbalitvet-ACIAR-TE11/2007 were each amplified in one or two fragments using reverse transcriptase-PCR (RT-PCR), and subsequently cloned into pGEM-T Easy TA cloning system. The sequencing result demonstrated high homology between respective clones but with several variations that were identified as single nucleotide polymorphisms (SNPs). A total of 1,707 base pair and 1,350 base pair of H5 and N1 genes respectively were successfully assembled from multiple clones containing the genes of interest. The features of both H5 and N1 genes from this isolate resemble the typical characteristics of Indonesian strains of H5N1 virus from sub-clade 2.1.3. Key Words: Avian Influenza, Characterization, Gene Cloning, Hemagglutinin, Neuraminidase

Utilization of oligosaccharides component from soybean meal and sweet potatoes on broiler feed Haryati, Tuti; ., Supriyati
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 4 (2010)
Publisher : Indonesian Animal Sciences Society

The investigation was carried out to study the effect of oligosaccharides from soybean meal (SBM) and sweet potatoes as feed additives on feed to improve the productivity of broiler. The amounts of 704 day-old chicks from Arbor Arcres were randomly distributed into 16 treatments with 4 repetionsÂ and with 11 chicks in each pens. The treatmentsÂ were: 2 level of sweet potato oligosaccharides (0.1 and 0.2%), 2 levels of residue ofÂ sweet potato extracts (0.4 and 0.8%), 2 levels of SBM oligosaccharides (0.1 and 0.2%), 2 levels of residue of SMB extracts, 2 levels of MOS commercial (0.01 and 0.02%) 2 levels of commercial inulin (0.1 and 0.5%), 2 levels of commercial FOS (0.2 and 0.4%) and control with and without antibiotic. The results showed that better FCR (P < 0.05) was seen on the addition of 0.2% sweet potato oligosaccharides at 3 weeks trial, meanwhile on 0.1% addition was able to improved the absorption of Ca and P. The trial up to 5 weeks, only treatment with 0.8% of residue have a better FCR value (P < 0.05). The effect of utilization of SMB oligosaccharides was only observed on 0.8% residue at 5 weeks trial which have a better FCR value. It could be concluded that the extract of oligosaccharides from soybean meal and sweet potatoes could improve the effisiency of broiler feed. Key Words: Oligosaccharides, Sweet Potato, Soy-Bean Meal, Productivity, Broiler

The Utilization of Indigofera sp as the sole foliage in goat diets supplemented with high carbohydrate or high protein concentrates Ginting, Simon P; Krisnan, Rantan; Sirait, Juniar; ., Antonius
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 4 (2010)
Publisher : Indonesian Animal Sciences Society

Indigofera sp is a tree leguminous forage species known to be adaptable to the drought climate and saline soil and so it has potential as alternative feed resource to support the ruminant animal production. This study aimed to study the Â responses of goats fed Indigofera sp as the sole foliages in their diets. Twenty male Boer x Kacang crossbred goatsÂ (6 months of age;16 Â± 2,1 kg BW) were randomly assigned to four dietary treatments containing fresh or wilted leaves of Indigifera sp. as the sole foliages and supplemented with high carbohydrate (HCC) or protein (HPC)concentrates. Consumable parts i.e. leaves and twigs of about 8 to 10 months of age of Indigofera sp. were harvested by hand plucking every day in the morning. The experiment was a 2 x 2 factorial design arranged in a completely randomized design. The ruminal pH ranged from 6.14-6.85 and were Â not affected (P > 0.05) by wilting nor by the type of concentrates. Wilting did not affect (P > 0.05) the concentration of Â ruminal NH3-N, butÂ it was significantly higher (P < 0.05) in HPC group (32.36-32.54 mg dL-1) than those in HCC group(17.94-23.57 mg dL-1). Total VFA concentration were not affected by wiltingÂ nor by the type of concentratesÂ (P > 0.05). Goats in the HCC group,however,numerically have higher total VFA concentration (178.5-183.75 mmol L-1) than those in the HPC group (142.21-174.64 mmol L-1). The apparent digestibility coeficients Â of DM, OM, CP and energy of the diet were not different (P > 0.05) when contained wilted or fresh Indigofera foliage.Â Significant increases (P < 0.05)Â Â in the DM and Â OM, but not in the CP and energy Â apparent digestibility coeficients were observed in the HPC goats. Dry matter intakes were not different (P > 0.05) among dietary treatments, but the ADG of goats in the HCC group (60-63 g d-1) were significantly lower (P < 0.05) than thoseÂ of goats in the HPC treatment (80-87 g d-1). Blood glucosa concentration was not affected by wilting nor by type of concentrates (P > 0.05). Â BUN was not affected by wilting process, but providing high protein concentrates significantly (P < 0.05) increased the BUN concentration. It is concluded that foliage of Indigofera sp could be used as the sole forage in intensive production of goats. Wilting the foliage prior to feeding seemed to be unnecessary, since this process does not improve the animal productivity in term of daily gain and efficiency of feed utilization. Key Words: Indigofera sp, Supplements,Â Ruminal Fermentation, Goats

Effects of concentration of Follicle Stimulating Hormone (FSH) on the rates of ovulations and twinning birth Situmorang, Polmer; Sianturi, R; Kusumaningrum, D. A; Triwulaningsih, E
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 4 (2010)
Publisher : Indonesian Animal Sciences Society

Twinning in monotocous domestic species dairy cattle is most frequently by dyzygote in which two eggs is ovulated by treatment with gonadotropin hormon. A study to see the effects of concentration of Follicle Stimulating Hormone (Folltropin) on the ovulation rates and twinning birth was conducted. The experiment designed was commpletely randomized designed with 3 treatment of total concentration of FSH (A: 12, B: 6 and C 3 ml) and 4 lactating cows for a replications. Hormon was intramussculary injected in decreasing dossis method twice a day with 12 hours apart for 4 days. Data recorded were the diameter of ovary (DO), total corpus luteum (TCL), concentration of progesterone on the day 12 of estrus cycle (P), percentage of pregnancy and number of birth. FSH significantly increased the ovulation rates. The means DO was significantly (P < 0.05) higher after injection (6.3 cm) than those before injection (2.0 cm). The TCL and P were highly significant (P < 0.01) higher after injection. The TCL and P were 1.2 and 0.8 ng/ml and 8.5 and 3.0 ng/ml before and after hormone treatment respectively. Concentration of FSH significantly increase (P < 0.05) the TCL and P but not for DO. The means DO, TCL and P were 5.0, 4.5 and 1.6, 5.5, 8,8 and 1.8 and 8.4cm, 12.3 and 5.3 ng/ml for treatments C, B and A respectively. Persentage of pregnancy was decreasing by the increasing of ovulations rates and the highest percentage of pregnancy 75% was obtained on treatments C. The normal single birth was found for treatment C and neonatal death were occurred for treatment B and A where the number of calves is higher than 2. In conclusion the FSH increased the ovulation rates in dairy cattle and the ovulation rates obtained was also affected by the concentration of hormone. Key Words: Hormone, FSH, Ovulation, Twin Birth

Aplication of growth hormone genes familly (GH, GHR, GHRH and Pit-1) for detecting genetic variation of buffaloes in Pandeglang and Lebak districts in Banten Province Sumantri, Cece; Diyono, R; Farajallah, A; Anggraeni, A; Andreas, E
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 4 (2010)
Publisher : Indonesian Animal Sciences Society

Selection using genetic markers are commonly performed to improve livestock productivity in the livestock industry. The objectives of this study were to identify growth hormone genes family (GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI) polymorphisms of Banten buffalo population consisted of Pandeglang and Lebak subpopulations. A total number of 209 blood samples were collected from 15 districts. Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques, then PCR products of GH, GHR, GHRH and Pit-1 Genes were digested with MspI, AluI, HaeIII and HinfI enzyme restriction. Fragments of GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI were detected by EtBr method. The results showed that GH|MspI and GHRH|HaeIII loci were polymorphic, GH|AluI, GHR|AluI and Pit-1|HinfI, loci were monomorphic. GH allele (-) at locus GH|MspI was only found in Cisata (0.03) and Menes (0.11). Allele B at locus GHRH|HaeIII only found in Cibadak (0.42), Cisata (0.30) and Menes (0.11). In the total population of Banten locus GH|MspI have low diversity (He = 0.02) and polymorphic information content (Pic = 0.02), whereas GHRH|HaeIII locus has a higher diversity (He = 0.23) and Pic (0.22). Key Words: Polymorphism, Growth Hormone Genes, Buffalo

In vitro study of larvacidal effect of Chrysomya bezziana between atsiri oil (Piper betle leaf) from Sri Lanka and Bogor Wardhana, April H; Muharsini, S; Santosa, S; Arambewela, L.S.R; Kumarasinghe, S.P.W
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 4 (2010)
Publisher : Indonesian Animal Sciences Society

Treatment of myiasis caused by the larvae of Chrysomya bezziana resulted variation results. The aim of the experiment are to compare larvacidal effect of atsiri oil of Piper betle leaf originated from Sri Lanka and Bogor agains Chrysomya bezziana larvae and to identify atsiri oil using Thin Layer Chromatography (TLC).Â Silica gel plate GF254 with toluena and ethyl ecetate as diluents was used in TLC. L1 and L2 of Chrysomya bezziana larvae were used for in vitro assay using agar plate contained atsiri oil of three concentrations ie. 2, 3 and 4%. Asuntol 1% and aquadest sterile were used as positive and negative control, respectively. Ten larvae were used in five replicates. The larvae were observed every 30 minutes for four hours. Larval mortality were counted and probit analysed using POLO-PC software, therefore the lethal concentration (LC50 and LC95) and lethal time (LT50 and LT95) were defined. The results showed there was a difference on migration distance between atsiri oil from Sri Lanka and Bogor on nRf 79-88 analysed using TLC. In vitro assay showed that both lethal concentration and lethal time of atsiri oil from Sri Lanka was higher than from Bogor. This results indicated that larvacidal effect of atsiri oil from Bogor was stronger than atsiri oil from Sri Lanka in in vitro both on L1 and L2. Key Words: Piper Bitle Leaf, Atsiri Oil, Chrysomya bezziana, Sri Lanka, Bogor

Purification and production of monospecific antibody to the hemagglutinin from Subtype H5N1 influenza virus Tarigan, Simson; Indriani, R; Hewajuli, D
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 4 (2010)
Publisher : Indonesian Animal Sciences Society

The purpose of this study was to purify the hemagglutinin from H5N1 virus and to generate monospecific antibody appropriate for production of sensitive and specific immunoassay for H5N1 avian influenza. For this purpose, a local isolate H5N1 virus (A/Ck/West Java/Hamd/2006) was propagated in chicken embryos. The viral pellet was dissolved in a Triton-X-100 solution, undissolved viral particles were pelleted by ultracentrifuge, and the supernatant containing viral surface glycoproteins (Hemagglutinin and neuraminidase) was collected. The neuraminidase in the supernatant was absorbed by passing the supernatant through an Oxamic-acid-superose column. After dialyzing extensively, the filtrate was further fractionated with an anion exchange chromatography (Q-sepharose) column. Proteins adsorbed by the column were eluted stepwisely with 0.10, 0.25, 0.25 and 0.75 M NaCl in 20 mM Tris, ph 8. Hemagglutinin (H5) was found to be eluted from the column with the 0.5 M NaCl elution buffer. The purified H5 was free from other viral proteins based on immunoassays using commercial antibodies to H5N1 nucleoprotein and neuraminidase. When used as ELISAâs coating antigen, the purified H5 proved to be sensitive and specific for hemagglutinin H5. Cross reactions with other type-A-influenza virus, H6, H7 dan H9, were negligibly low. For the production of monospecific antiserum, the purified H5 was separated with SDS-PAGE, the band containing the H5 monomer was cut out , homogenised and injected into rabbits. The antiserum was capable of detecting the presence of inactivated H5N1 virus in a very dilute suspension, with a detection limit of 0.04 heagglutination (HA) unit. The purified hemagglutinin and the serum raised against it should be useful for developing specific, sensitive and affordable immunoassay for H5N1 avian influenza. Key Words: H5N1, Hemagglutinin, Triton, Oxamic-acid Sepharose, Q sepharose, ELISA

Effect of mixing banana stem juice and subsequent heating on rumen degradability of soy bean meal Yulistiani, D; Puastuti, W; Mathius, I.W
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 1 (2010)
Publisher : Indonesian Animal Sciences Society

The study was conducted to evaluate the rumen degradability of soy bean meal when it was mixed with banana stem juice and was subsequently heated.Â Banana stem juice was used as source of tanin to protect protein in soy bean meal from degradation in the rumen. The banana stem juice (in volume) was mixed with soy bean meal at three ratio, which were 1 : 1, 1:2 and 1 : 3 (w/v). These mixing was then heated at 60oC and 90oC. The heating was done in an oven. Sample from each treatment was then filled into polyester bag with porosity of 45 mm and the size of 5 x 9 cm. The bags were incubated in the rumen of fistulated sheep for 0, 2, 4, 8, 16, 24 and 48 hours. The experiment was carried out in factorial 3 x 2, with three levels of banana stem juice and 2 levels of heating and was arranged in completely randomized design. Untreated soy bean meal was also incubated used asÂ control. Degradation data of dry matter and crude protein at each incubation time was fitted into exponential equation,Â P = a + b (1 - ect). Results shows that there was a significant interaction factor of stem juice ratio and heating on water soluble substrate (constant A) where heating at 90oC with ratio 1 : 2 (w/v) resulted in the highest a. The insoluble but fermentable substrate (B) was decreased by the increasing concentration of banana stem juice, while the rate of protein degradation of b fraction was lower compared to control. In conclusion, banana stem juice can reduce the rate of degradation of soy bean meal in the rumen. Key words: Degradation, Crude Protein, Soy Bean Meal, Banana Stem Juice

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Ahmadi Riyanto

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medpub@litbang.deptan.go.id

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Journal Mail Official
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Kota bogor,

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