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Journal of the Indonesian Tropical Animal Agriculture
Published by Universitas Diponegoro
ISSN : 20878273     EISSN : 24606278     DOI : -
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers
Articles 676 Documents
Association of growth hormone (GH) gene polymorphism with growth and carcass in Sumba Ongole (SO) cattle Agung, P. P.; Anwar, S.; Putra, W. P. B.; Zein, M. S. A.; Wulandari, A. S.; Said, S.; Sudiro, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.153-159

Abstract

A study was conducted to identify the polymorphism in the intron 3 of the Growth Hormone (GH) gene and also to evaluate the association of the GH gene polymorphism with growth parameters and dressing percentage in the Sumba Ongole (SO) cattle. A total of 267 individual DNA samples were used in the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The SO cattle growth parameters data (n=44) including birth weight (BW), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365) and also dressing percentage (DP) (n=122) were investigated in this study. There were three genotypes (AA, AB, and BB) of the GH gene based on the PCR-RFLP analysis with allele frequency was 0.87 and 0.13 for A allele and B allele respectively. The highest genotype frequency in the SO cattle is AA (0.76) and the lowest is BB (0.02). The Heterozygosity Observed (Ho) value in the SO cattle population is 0.23 and Polymorphism Information Content (PIC) value is 0.20. Therefore, the genetic diversity in the SO cattle based on the GH gene polymorphism is quite low. There is no association (P>0.05) in BW, WW205, YW365, and DP with genotypes of the GH gene. As the result, the GH gene in this study cannot be used as a genetic marker in the SO cattle breeding program.
THE EFFECT OF CONCENTRATE CONTAINING PROBIOTICS ON FERMENTATION CHARACTERISTICS, METHANOGENESIS AND In vitro NUTRIENT DIGESTILITY Santoso, B.; Hariadi, B. Tj.; Abubakar, H.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.210-216

Abstract

The aim of the experiment was to evaluate the effect of probiotic addition in concentrate onfermentation characteristics, methane (CH4) production and in vitro nutrient digestibility. Two strainslactic acid bacteria (LAB) i.e. Lactobacillus plantarum and Lactobacillus acidhophilus, and one strainyeast of Saccharomyces cerevisiae was used as probiotic.This experiment was arranged in a completelyrandomized design consisted of 4 treatments as follows grass silage (G); grass silage + concentratewithout probiotic (G+A); grass silage + concentrate containing L. plantarum and. S. cerevisiae (G+B);grass silage + concentrate containing L. acidophilus and. S. cerevisiae (G+C); grass silage + concentratecontaining L. plantarum and L. acidophilus (G+D). Data were analyzed as completely randomizeddesign and followed by Duncan’s multiple range test. The results showed that the concentrate containingLAB varied 1.5 × 106 and 3 × 107 cfu/g, and 3 × 103 cfu/g of S. cerevisiae. Combination between L.plantarum and S. cerevisiae (G+B), and L. acidophilus and S. cerevisiae (G+C) in concentrate increased(P<0.01) propionic acid proportion. The average CH4 production in concentrate containing probiotic(G+C, G+D and D+E) was lower by 6.9% (P<0.01) compared to concentrate without probiotic (G+B). The in vitro dry matter (DM) and neutral detergent fiber (NDF) digestibility were higher (P<0.01) by25.7% and 6.3% respectively, in grass silage substrate with concentrate containing probiotic (G+C, G+Dand G+E) than in grass silage with concentrate without probiotic (G+B). In conclusion, addition ofprobiotic in concentrate could increase in vitro propionic acid proportion, DM and NDF digestibility andreduce CH4 production.
MICROBIOLOGICAL AND PHYSICAL PROPERTIES OF BEEF MARINATED WITH GARLIC JUICE Nurwantoro, N.; Bintoro, V.P.; Legowo, A.M.; Ambara, L.D.; Prakoso, A.; Mulyani, S.; Purnomoadi, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 3 (2011): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.3.166-170

Abstract

The study was carried out to investigate the effect of marination, performed by soaking of beef ingarlic juice, on microbiological and physical properties. The study was committed to a completelyrandomized design, with 5 treatments, i.e.: T0 (unmarinated beef, as a control), T1, T2, T3, and T4 thatbeef were marinated in garlic juice for 5, 10, 15, and 20 minutes, respectively, at room temperature(25⁰C). Each treatment consisted of 4 replications. Examination upon experimental parameters wasconducted after marinated (and control) beef was stored for 8 hours at room temperature. Total bacteria,total coliform and water holding capacity of beef were significantly (P<0.05) affected by marinationwith garlic juice. Conversely, cooking loss was not significantly affected (P>0.05) by the treatments. Asa conclusion, marination of beef with garlic juice could reduce total bacteria, total coliform, and waterholding capacity, but could not reduce cooking loss.
Inclusion effect of ginger and turmeric mixture combined with Lactobacillus spp. isolated from rumen fluid of cattle on health status and growth of broiler Risdianto, D.; Suthama, N.; Suprijatna, E.; Sunarso, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 4 (2019): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.4.423-433

Abstract

This study was conducted to evaluate the combination of ginger and turmeric extracts added with Lactobacillus spp. (GTL) on body resistance and productivity of broilers. 576 broilers with an initial body weight of 36 ± 0.99 g were used and were observed for 35 days. GTL containing ginger extract (0.2 g/liter), turmeric extract (0.4 g/liter) and Lactobacillus spp. (2,997 x 107 cfu/ml), and was given since 1-day old. Parameters observed were bursa fabriscius, spleen, and thymus weights, gluthationine peroxidase (GSH-Px), superoxide dismutase (SOD), Lactobacillus spp., Coliform, and Eschericia coli, feed consumption, body weight gain, and feed conversion ratio (FCR). A completely randomized design (CRD) was assigned with 3x4 factorial pattern (3 types of feed, 4 levels of GTL), with 4 replications (12 birds each). First factor, types of feed (A): self-formulated feed (A1), combination of self-formulated and commercial feed with 50 portion each (A2) and commercial feed (A3). Second factor, levels of GTL (B): 0 ml (B0), 2 ml (B2), 4 ml (B4) and 6 ml (B6) per litre drinking water. Data were subjected to ANOVA and continued to Duncan test at 5% probability. Results indicated that self-formulated feed with inclusion of GTL at 4 ml (A1B4) improve body resistance and increase production performance of broilers.
The expression of heat shock protein 70 gene with organic selenium supplementation and its effetc on productivity of broilers in tropical environment Amizar, R.; Suharti, S.; Jakaria, J.; Mutia, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 4 (2017): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.4.279-287

Abstract

The purpose of this experiment is to study the effect of organic selenium (Se) supplementation on the expression of heat shock protein 70 gene (HSP70), glutathione peroxidase (GSH-Px) enzyme activity, malondialdehyde (MDA) and productivity of broilers in tropical environment. Three kinds of environmental pens were designed in this experiment: comfortable environment pens with temperature of air conditioner adjusted at 22oC (R0), tropical environment pens (±30oC ) without organic Se (R1), and tropical environment pens supplemented with 0.30 ppm organic Se (R2). One hundred and twenty broiler chickens (unisex) were used in this study. There were 40 chicks per pen for each treatment. The experimental design was completely randomized with four replications for each treatment. The data were statistically analyzed using the general linear model of SAS program. Results showed that R0 and R2 groups had significantly increased (P<0.05) feed intake, body weight, body weight gain, and decreased feed conversion ratio compared to R1 groups. Meanwhile, the expression of HSP70, GSH-Px enzyme activity and MDA of R2 groups and R0 groups were significantly lower (P<0.05) than that of R1 groups. It was concluded that the broilers given 0.30 ppm organic Se in tropical environment had similar productivity and expression of HSP 70 with broilers kept in comfortable environment.
NUCLEAR MATURATION RATE OF SHEEP OOCYTES IN VITRO: EFFECT OF STORAGE DURATION AND OVARY TEMPERATURE Febretrisiana, A.; Setiadi, M. A.; Karja, N. W. K.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 2 (2015): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.2.93-99

Abstract

The present study was conducted to investigate the effects of duration and temperature storageof sheep ovaries on nuclear maturation in vitro of oocytes obtained from stored ovaries. Ovaries werecollected from slaughterhouse and stored in physiological saline for 2-4 h, 5-7 h and 8-10 h at varioustemperatures (27-28°, 36-37° and 4°C). Cumulus oocyte complexes (COCs) were collected with slicingmethod from each group and matured in vitro for 26 h. There was no difference between the proportionsof oocytes maturation to metaphase II when collected from ovaries stored at 27-28°C and 36-37°C for 2-7 h of slaughter. However, the percentages of oocytes from ovaries stored at 4°C were significantlylower (P<0.05) than those stored at higher temperature (69.23%, 70.83% and 45.65%, for 27-28, 36-37and 4°C at 2-4 h, respectively; 59.61%, 64,58% and 36.36% for 27-28, 36-37 and 4°C at 5-7 h,respectively). The proportion of oocytes meiosis to metaphase II (MII) were significantly (P<0.05)decreased when the ovaries stored at 27-28 and 36-37°C for 8-10 h, but not in oocytes stored at 4°C(24.37%, 7.84% and 45.23%, respectively). The maturation rate of oocytes from ovary stored at 4°C washigher than those collected from ovaries stored at higher temperature (P<0.05). This finding indicated that the storage of ovaries at 4°C for 8-10 h is effective for maintaining the development competence ofsheep oocyte better than storage at higher temperature.
TOTAL NON-STRUCTURAL CARBOHYDRATE (TNC) OF THREE CULTIVARS OF NAPIER GRASS (Pennisetum purpureum) AT VEGETATIVE AND REPRODUCTIVE PHASE Budiman, B.; Soetrisno, R.D.; Budhi, S.P.S.; Indrianto, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 2 (2011): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.2.126-130

Abstract

An experiment was conducted to determine Total Non-structural carbohydrates (TNC) of threecultivars of napier grass (Pennisetum purpureum) harvested at vegetative and reproductive phases. Thecultivars tested were Taiwan (Gt), King (Gk) and Mott (Gm) and arranged in a 3 x 2 of treatments withfour replicates following nested design. The results showed that the highest sugar content (P<0.01) wasfound in Gt cultivar and the lowest was in Gm cultivar. The highest starch content (P<0.01) was found inGk cultivar and the lowest was in Gt cultivar. TNC content of Gt and Gk cultivars were not significantlydifferent, but both were significantly higher (P<0.01) compared with the Gm cultivar. It can beconcluded, that there were differences in TNC between cultivars, however, the TNC content in Gkcultivar was not different with Gt cultivar, while Gm cultivar have the lowest (P<0.01) TNC content. Atreproductive phase all cultivars have higher (P<0.01) TNC and starch content than at vegetative phase
Effect of different transportation period on body weight loss, hematological and biochemical stress responses of sheep Lendrawati, L.; Priyanto, R.; Jayanegara, A.; Manalu, W.; Desrial, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 2 (2020): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.2.115-123

Abstract

The study was conducted to evaluate the duration of road transportation during 4, 8 and 12 hours on body weight loss, hematological and biochemical responses of sheep. Twenty-four male Javanese thin tailed sheep were separated into transported group (T4, T8 and T12) and non-transported group (NT4, NT8 and NT12). Transportation (T) groups were transported for 4, 8 and 12 h in the same open pick up with 0.28 m2/head of loading density. Body weight, haematological and biochemical blood profiles were determined. The results showed that increasing of transportation duration significantly increased (P<0.05) body weight loss and decreased (P<0.05) hematocrit, cortisol and glucose values in the blood. However, these values were in the normal range of sheep well-being. In conclusion, transported healthy Javanese thin tailed sheep under good condition without feed and water access up to 12 h can tolerate to adverse effect of transportation without disturbing their welfare.
THE ANALYSIS OF FACTORS AFFECTING THE PERFORMANCE AND BENEFIT OF KARAPAN (RACING) CATTLE BUSINESS IN MADURA ISLAND, EAST JAVA, INDONESIA Riszqina, Riszqina; Isbandi, Isbandi; Rianto, E.; Santoso, S.I.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 1 (2014): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.1.65-72

Abstract

A research was carried out to analyze the influence of productive factors on the performance ofkarapan (means racing) cattle business in Madura Island, East Java Province, Indonesia. The researchwas conducted by a survey method, with 135 karapan cattle farmers as respondents in regencies ofBangkalan, Sampang, Pamekasan and Sumenep (mainland). The data were collected in the period ofApril to August 2012. Data of zootechnique indicators variables, farmer’s motivation, allocation time oflabour, labour skills, business scale, productivity of karapan cattle, farmers' performance and benefit ofkarapan cattle business were analysed by Lisrel 8.8 program. The results showed that factors ofzootechnique, farmer’s motivation, labour skills and business scale had highly significant influence(P<0.01) on productivity of karapan cattle, but allocation time of labour did not have significantinfluence (P>0.05). The performance of karapan cattle business was highly influenced by productivity ofkarapan cattle (P<0.01). The benefit of karapan cattle business was influenced (P<0.01) by performanceof karapan cattle business. It is concluded that the farmer's benefit of karapan cattle business wasinfluenced by performance of karapan cattle, which in turn was influenced by productivity of thekarapan cattle.
Effect of binder mineral in batchery waste based feed pellet on its proximate component and energy values Sulistiyanto, B.; Utama, C.S.; Sumarsih, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.107-114

Abstract

The Experiment was conducted to study effects of binder mineral addition to the hatchery waste based pelletprocessingon its proximate component and energy value. The study may be beneficial to emerge a new feed resource for local farm businesses. The hatchery wastes those comprised of 30% of egg shells, 60% of un-hatched eggs, and 10% of culled DOC were blended, and mixed with 10% of cassava cake meal(W/W), then mixed well with mineral adsorbent (bentonites or zeolites) at 0, 1, 2, 3, 4 and 5% (W/W). The mixture was conditioned at 80 ºC for 15 min, and extruded to make a pellet with 6 mm of diameter and 30 mm in length, and dried into warm air flow dryer. The moisture, crude protein (CP), ash, ether extract (EE), crude fiber (CF), apparent metabolizable energy (AME), true metabolizable energy (TME), metabolizability(MET) and nitrogen retention (NR) were observed in each pellet. Result of the experiment showed that moisture of pellet was decreased (P<0.05) by zeolites or bentonites addition. Even though the effect ofbinder mineral addition gave different patterns, concentrations of ash, crude protein, ether extract and crude fiber of pellet product were increased by increasing level of mineral adsorbent (P<0.05). Nutrient availability as was represented by AME, TME, MET, and NR were increased by zeolites and bentonites additions (P<0.05). In conclusion, supplementations of bentonites and zeolites in the hatchery waste based pelletcould improve its proximate component concentration and nutrient availability.

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