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Biomedical Engineering
Published by Universitas Brawijaya
ISSN : -     EISSN : -     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Arjuna Subject : -
Articles 27 Documents
 1   2   3 
Comparing the spectral profiles of the javanesse gending with the classical music as the therapeutical music Nurida Finahari; Gatut Rubiono; Gatot Soebiyakto
Biomedical Engineering Vol 2, No 1 (2016)
Publisher : Biomedical Engineering

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Abstract

Objective:  So this study is trying to analyze the potency of Javanesse Gending as the therapeutic music. It is a further exploration of the finding state that the Gending Tejanatha has the spectral characteristic similarity with The Magic Flute of Mozart. Material and methods: The study was done by analyze the audio characteristics patterns of five Javanesse Gending (JG) compare with the  five classical music (CM) of Mozart and Beethoven. The audio characteristics were obtained from the sound analyzer program. The results were in form of spectral graphics. The parameters include spectral envelope, sound intensity and frequency.Result:  JG and CM have different pattern for the left and the right audio. This indicate difference stimulus for the neural, that will appear as cross references for primary audiory area. The frequencies of JG is higher than CM. The frequency and envelope indicate the variation of emotion that will be induced. It represent the sound colour (timbre). The JG timbre is determined by the material of the instruments, used to made from copper or iron. This material does infuence the emotional responses, especially to the player (pengrawit). The influence of the timbre, not as intens as the influence of the musical tempo that relate with the rhythm harmony. In the JG, the musical tempo represent the soul of this music. It is very essential. The tempo changes from the  usual pattern will raise the anxiety.Conclusion: It can be concluded that the JG has the same potency as the CM to be used as therapeutic music. The JG has the higher potency to influence emotional state than the CM due to its envelope profile.
The effect of polyphenol to visceral fat profile protein at obesity model rat Sri Rahayu Lestari; Nuning Wulandari; Siti Imroatul Maslikah
Biomedical Engineering Vol 2, No 1 (2016)
Publisher : Biomedical Engineering

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Abstract

Objective: To asses visceral fat profile protein between normal and obesity model rat. Material and Methods: Normal and obesity rat model have been treated with polyphenol from rambutan peel extract for 12 weeks. Rat were divided into 2 major group  based on their weight which normal rat and obesity model rat respectively have average weight 180-200 gram and 360-380 gram. This treatment were divided into minor group which are placebo (without treatment),  treatment with ellagic acid and treatment with polyphenol (dosage 15 mg/kg body weight; 30 mg/kg body weight and 60 mg/kg body weight. Rat were sacrificed after 12 weeks treatment to asses visceral fat profile protein and continue with running SDS PAGE12,5 %. Protein molecular weight sample were calculated with regression analysis between marker protein mobility and logarithm from marker. Band protein analysis were analyze qualitative with SDS PAGE meanwhile quantitative analysis with Gel Doc (Bio Rad). Density band protein were analyze were using Quantity One software and confirmed with Western Blotting.Result: Protein profile characteristic normal rat and obesity-model rat were in range between117-20 k Da. The amount of band protein were found in normal rat were less than the amount of protein at obesity-model rat. Conclusion: There were difference molecular weight at protein density 57 k Da for obesity model rat which has been treated with rambutan peel extract.
A Cross Correlation Between Karyotyping and Anthropometric Measurement of Down Syndrome at Malang, Indonesia Antonius Christianto Christianto; Haryudi Aji Cahyono; Djoko Wahono Soemadtji; Fatchiyah Fatchiyah Fatchiyah
Biomedical Engineering Vol 2, No 2 (2016): Biomedical Engineering
Publisher : Biomedical Engineering

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Abstract

Objective:   Herein, we reported a first study of Down syndrome chromosome analysis in Malang, Indonesia and correlation between phenotype, anthropometric measurement and karyotyping dataMaterial and methods: Karyotyping was conducted using peripheral blood culture.Result:   The karyotyping result showed that from nine patients with DS, we found two types of DS, trisomy 21 in seven patients and mosaicism in two patients. Interestingly, two patients with mosaicism have different percentages of cells with abnormal chromosomes (trisomy 21). In our cases, lower percentages of cells with abnormal chromosomes, the morphologies of patient were more likely normal control.Conclusion:    At least in, at least in part the ratio between lower and upper segment was proved to be different between normal and DS patients.
Cytogenetics profile of student with syndromic mental retardation on special schools in Banjarmasin Siti Wasilah; Husnul Khatimah; Tri Indah Minarni
Biomedical Engineering Vol 2, No 1 (2016)
Publisher : Biomedical Engineering

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Abstract

Objective:  This descriptive study aims to describe the cytogenetic profile of students with mental retardation disorders on special school in Banjarmasin conducted since 2012-2013.Material and methods:  Cytogenetic profile described based the existence of free trisomy 21, trisomy 21 with translocation, mosaic trisomy 21 and trisomy 21 partially or in the form of numeric aberration and structure of chromosomal abnormalities such as fragile X, specific deletions of chromosomal segments or the presence of a ring chromosome forms.Result:  The results obtained from the study of 22 students with physical signs of mild to severe syndromic mental retardation. The results of cytogenetic examination showed most of the sample (77.78%) with free trisomy 21 (karyotype 47, XX, + 21 or 47, XX, + 21) or a classic type of Down syndrome, 1 sample with mosaic karyotype: 46, XY (1%) / 47, XY, + 21, 1 sample with structural abnormalities of chromosomes 22 (karyotype: 46, XY, ring 22) and 2 samples with normal karyotype (karyotype: 46, XY or 46, XX).Conclusion:  Cytogenetic profile of students with syndromic mental retardation on special school in Banjarmasin are free trisomy 21, mosaic trisomy 21, structure aberration (ring chromosome 22), and a normal karyotype.
Detection of Hepatitis C Virus RNA in Blood Donors with Nested PCR Technique Supiana Dian Nurtjahyani; Mochammad Amin Amin; Retno Handajani Handajani
Biomedical Engineering Vol 2, No 2 (2016): Biomedical Engineering
Publisher : Biomedical Engineering

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Abstract

Objective: This study was conducted to detect hepatitis C virus (HCV) RNA among blood donors in Tuban, East Java, Indonesia,  using nested Polymerase Chain Reaction (PCR) technique.Material and methods: Seven blood donors  with positives anti-HCV were obtained from 500 blood donors from Red Cross Blood Donor Center (Palang Merah Indonesia = PMI) Tuban, East Java, Indonesia, consisting of 375 men (75%) and 125 women (25%). Detection of anti-HCV antibody prevalence in the blood donors was done by using Enzyme-Linked Immunosorbent Assay (ELISA).  The detection of HCV RNA in blood donors  sera  with positives  anti-HCV were performed using nested-PCR technique with three set of primer pairs targeting the NS5B and 5’UTR regions of HCV.  Further amplification products were visualized on a 2% agarose gel containing ethidium bromide under  UV illumination.Result: Seven positives anti-HCV blood donors sera (1.4%) were obtained from 500 blood donors sera, in PMI Tuban, East Java, Indonesia. Out of seven blood donors sera with positives anti-HCV, 6 sera (85,7%)  were positives HCV RNA by using nested PCR technique. Five HCV RNAs were detected with two set of primers based on NS5B HCV region and 1 positive HCV RNA was detected with another set of primer based on 5’UTR HCV region.Conclusion: HCV RNAs were detected in 85,7% positives anti-HCV blood donors using nested PCR technique, in PMI Tuban, East Java. Further research is needed to determine the  genotype  and  subtype  of  HCV. 
The effect of lecithin on liver function of white rats (Rattus norvegicus) induced carbon tetrachloride Lestari Dewi
Biomedical Engineering Vol 2, No 1 (2016)
Publisher : Biomedical Engineering

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Abstract

Objective: To prove that the lecithin is administered orally has hepatoprotective effect on white male rats given CCl4 intraperitoneally.Material and Methods: 45 male Wistar rats, aged 12 weeks were randomly divided into five groups. Group I, given solvent CMC - Na 0.25% dose of 0.01 ml/g bw, and were given olive oil dose of 1 ml/kg bw, intraperitoneal injection. Group II, given solvent CMC - Na 0.25% dose of 0.01 ml/g bw, and given CCl4 dose of 1 ml/kg bw,dissolved in olive oil by intraperitoneal injection. Group III, IV and V respectively given dose lecithin 90/180/360 mg/ kg bw,dissolved with 0.25% CMC-Na, and  given CCl4 dose of 1 ml/kg bw dissolved in olive oil at a ratio of 1: 1, by intraperitoneal injection. On day 10, 24 hours after administration of CCl4 and olive oil , all the rats dissected, after first anesthetized with ether by inhalation. Then the blood taken from the heart to examine the level of AST and ALT in serum and hepar were taken for  histopathological examination.Results: Giving  lecithin 90/180/360 mg/kg bw were accompanied CCl4 1 ml / kg body weight (group III, IV, and V), shown to reduce levels of SGOT in serum compared with the group that received only CCl4 and giving lecithin 90/180/360 mg/ kg bw with CCl4 1 ml/kg bw  (group III, IV, and V) is proven to reduce ALT levels compared with the group that received only CCl4. On histopathological examination of liver cells proves that lecithin dose 90 / 180/360 mg/kg bw with CCl4 1 ml/kg bw is proven to reduce the amount of cell necrosis than the group that only received CCl4.Conclusion: Lecithin has a protective effect against the liver function.
Effectiveness Of Isometric And Range Of Motion (Rom) Exercise Toward Elderly Muscle Strenght In Pasuruan Integrated Service Unit, Elderly Social Services In Lamongan Nurus Safa'ah; Ika Desti Srimurayani
Biomedical Engineering Vol 3, No 1 (2017): Vol. 3 No. 1 Biomedical Engineering
Publisher : Biomedical Engineering

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Abstract

Objective: Aging is a physiological process that will reduces the function of all organs, among other the musculoskeletal system that cause decreased muscle strenght. Under less of the muscle strenght will reduces activities and mobilization ability. Isometric and Range Of Motion (ROM) exercise is efficient alternative exercise for the elder who will begin the exercise program. This study was aimed to explain the difference of effectiveness between isometric and ROM exercise toward elderly muscle strenghtMaterial and methods: This study used to Pre Test and Post Test Design with time period approach involved 36 respondents, taken by purposive sampling. They were divided in to 2 groups; one group of 18 respondents underwent isometric exercise and the other group of 18 respondents did ROM exercise. Data were collected by checklist. Data were analyzed using Mann Whitney Test with level of significance α ≤ 0,05.Result:  Based on the result of research there were 8 (44,4%) respondents who had increased muscle strength after did isometric exercise, and were 14 (77,8%) respondents who had ones with ROM exercise. The analysis result using Mann Whitney Test showed p = 0,044 it means that H0 is rejected, so there are difference of effectiveness between isometric and Range Of Motion (ROM) exercise toward elderly muscle strenght. Range Of Motion (ROM) exercise was proven more effective to increase elderly muscle strenght who have normal movement, while isometric exercise was recommended to maintain muscle strength for the elderly who get the injured extremity and contraindications to do movement.Conclusion:  It can be used as a program to create healthy elderly and using optimally their time to do better things, such as doing ROM and isometric exercise.

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