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Research Journal of Life Science
Published by Universitas Brawijaya
ISSN : -     EISSN : 23559926     DOI : https://doi.org/10.21776/ub.rjls
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 9 Documents
 1 
Search results for , issue "Vol 8, No 3 (2021)" : 9 Documents clear
Diversity and Abundance of Insect on Shallot (Allium Ascalonicum L.) with Integrated Pest Management (IPM) and Conventional Patterns Manna, Dorkas Wantiawati; Afandhi, Aminudin; Tarno, Hagus
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.3

Abstract

Research on diversity and abundance of insect on shallot with Integrated Pest Management (IPM) and conventional patterns has been carried out. This study aims to analyze diversity and abundance of insects, damage level of shallot, and relationship between diversity and abundance of insects with growth and production of shallot in IPM and conventional land. This research was conducted in Junrejo village, Batu City in September to December 2017 while identification of insect was carried out at the Laboratory of Plant Pest and Disease, University of Brawijaya. This study uses Yellow pan trap and Pitfall trap methods. The result showed diversity index (H') of IPM land was 2.50 and of conventional land was 2.67, which both are moderate diversity. The abundance of insect on IPM land is higher (8672 individuals) compared to conventional land (3475 individuals). The level of crop damage is lower in IPM land compared to conventional land, while shallot growth and production is higher in IPM land compared to conventional land.
Indigenous Endophytic Bacteria Potentials to Control Black Rot Disease on Cabbage Towards the Development of Organic Vegetables Santoso, Verisca Agil Winanda; Ramadhani, Fatchuliani Safitri; Apriyani, Aliffia Nuraita; Aini, Luqman Qurata
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.8

Abstract

Organic farming plays an important role in achieving SDGs 2030, especially on point 2, 3, 13 and 15. Nevertheless, agriculture commodity often meets challenges in organic farming due to plant pests, for instance: on cabbage. Black rot disease on cabbage causes serious losses up to 70%. Most of farmers use chemical pesticides which leads to lots of negative impact towards the environment. Thus, its essential to control the environmentally friendly by applying bio control agents of indigenous endophyte bacteria instead. This article was aimed to examine the indigenous endophyte bacteria ability which excellences on organic cabbage as well as generating black rot caused by the bacterial pathogen X. campestris. Narrative reviews along was done through finding out the related material from International Journal articles. The result showed that there were indigenous endophyte bacteria which was able to be bio control agents towards black rot disease on cabbage, namely Pseudomonas fluorescens, Bacillus subtilis, and Streptomyces sp with effectivity level between 37-63% within resistance zone 1.44 up to 2.4 cm. Indigenous endophyte bacteria on organic cabbage had various kinds antagonist agents especially on Bacillus sp. and Pseudomonas sp. that able to resist the disease pathogen both in vitro and in vivo.
Fungal Endophytic Beauveria bassiana in Chinese Kale Against Plutella xylostella (L.) Larvae Afandhi, Aminudin; Ikawati, Silvi; Baroro, Ismatul; Afiyanti, Mufidah; Sari, Rose Novita; Handoko, Handoko; Suyono, Tri; Setiawan, Yogo; Tarno, Hagus
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.4

Abstract

This study evaluated three methods of inoculation of Beauveria bassiana endophytes from Chinese kale and then infected into Plutella xylostella L to determine the mortality rate. The endophytic B. bassiana using three different inoculation methods including seeds-soaking, leaf-spraying, and soil-wetting. The Chinese kale seeds from Winsa variety, B.bassiana isolates derived from collection planthopper. The endophytic fungus was identified to be B. bassiana based on the analysis of colony morphology. The results of this study included the degree of colonization of Chinese kale plants and the mortality of P. xylostella. The average B. bassiana colonies in leaves were 13.89%, stems 2.77% and roots 5.55%. The results showed on leaves that the seeds-soaking method obtained higher colonization of B. bassiana rate when compared to leaf-spraying and soil-wetting. The highest percentage of P. xylostella mortality was generated from seeds-soaking (45%), soil-wetting (37.5%), leaf-spraying (35%). The B. bassiana fungus which caused the fastest LT50 with a seeds-soaking (409.48 hours), and then soil-wetting (679.15 hours), leaf-spraying (1090.21 hours). The positive effects of seeds-soaking of endophytic B. bassiana and mortality of Plutella xylostell.
The Effect of Hermetia illucens larvae Formulation as Immunostimulant on Non-Specific Immune Response of Cyprinus carpio Challenged by Aeromonas salmonicida Mauludia, Farida; Maftuch, Maftuch; Nurin, Febi Nadhila; Sebastian, Andhang
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.9

Abstract

Cyprinus carpio is one type of freshwater fish. In Indonesia, carp outbreaks in carp since June 2002 have raised concerns, especially for fish farmers. One of the factors causing the outbreak of this disease is the bacteria Aeromonas salmonicida, which can cause fish to get sick and even die. Immunostimulants with natural ingredients that are environmentally friendly and can be used to increase the immune response of fish, one of which is maggot. The data analysis used in this study was a simple completely randomized design (CRD) with 6 treatments difference in the dose of maggot as an immunostimulant (0%, 25%, 50%, 75%, 100%) and three replications. The independent variable is the difference in the dose of maggot as an immunostimulant. The dependent variables include survival rate, total leucocytes, differential leukocytes, and water quality (pH, temperature, and DO). The FT-IR test results obtained that the best compound content in maggot flour is flavonoid compounds where these compounds can increase immune response. So, it is proven by phytochemical tests with positive maggot flour containing flavonoids, tannins, and alkaloids. In total erythrocytes, hematocrit, hemoglobin, total leukocytes, and differential leukocytes showed the best immune response in treatment C with a dose of 75% maggot.
Nutrient Composition of Red and White Cultivars of Dried Moringa oleifera Leaves from Probolinggo, Indonesia Mudita, Dhaniar; Harijono, Harijono; Widjajanto, Edi
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.5

Abstract

Moringa oleifera is a popular plant for combating malnutrition that grows well and is commonly consumed by Indonesian as a vegetable, but local varieties' nutrient composition never was studied before. Methods: Proximate, amino acids, minerals, vitamin C, and total phenolic composition of red and white cultivars of Moringa oleifera leaves from Probolinggo were determined on a dry weight basis based on AOAC methods. Results: Crude protein and fat were higher in white cultivar (35.36%, 6.25%). Total Amino Acid in both cultivars (83.06 g/100g for red and 82.86g/100g for white) were higher than in previous studies. Total Essential Amino Acid (TEAA) percentage of both of the cultivars was 46-49% and met the ideal protein needs of children (26%) and adults (11%). Iron, Zinc, Copper, Calcium, and Manganese was higher in white cultivar (36.21±0.08 mg/kg, 16.32±0.02%, 6.76±0.08mg/kg, 1.56±0.00 mg/kg, 7.11±0.00 mg/kg), while Magnesium, Potassium, Sodium, Phosphorus was higher in red cultivar (0.61±0.00%, 0.89±0.00%, 0.65±0.00%, 0.99±0.00). The content of vitamin C and total phenols was higher in the red cultivar (4162.8mg/kg and 239.8mg/kg). Conclusions: The red cultivar were best in 10/22 parameters (45%), white cultivar was best in 14/22 (55%). Those characteristics can contribute significantly to the nutrient requirements for protein and energy malnutrition treatment.
Neutrophil Gelatinase-Associated Lipocalin (NGAL) in Urine as a Potential Non-Invasive Method for Determine Kidney Damage in Predialysis Patients Titisari, Nurina; Hernowati, Tinny Endang; Ratnawati, Retty; Fauzi, Ahmad; Gunawan, Atma; Jaya, Wiwi
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.1

Abstract

NGAL expression in acute kidney failure is well known. It is approved that NGAL expression occurs earlier than the level of BUN and creatinine in acute kidney failure. NGAL is not only expressed in the blood but also in the urine, where urine collection has many advantages over blood collection. This study aims to observed the expression of NGAL in predialysis patients and to determine the correlation between NGAL serum and NGAL urine of patients. The sample was taken from healthy persons as control and predialysis patients. The examination of BUN, creatinine, and urinalysis were done to approve the diagnose in predialysis patients. While NGAL in serum and urine were analyzed using the Enzyme-Linked Immunoabsorbent Assay (ELISA). The results showed that the concentration of NGAL in serum was higher in the predialysis patients compared to the healthy subjects (p<0.05). There was a strong positive correlation between the NGAL in the serum and the NGAL in the urine (r= 0.98 and p<0.000). It is concluded that the non-invasive examination of NGAL in urine can be choose rather than using serum NGAL. However, it must be noted that NGAL could be used for chronic renal failure in predialysis patients as long as other biomarkers have been proven.
The Ambulance Location Can Influence Emergency Medical Service Response Time: A Literature Review Sanjana, I Wayan Edi; Wihastuti, Titin Andri; Muslihah, Nurul
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.6

Abstract

Factor of the accident was the highest cause of mortality. The mortality or death caused by accident could be avoided through a fast and appropriate first aid. The ambulance was a component of EMS that must reach the incident location in a quick time. This research was aimed to identify the effects of ambulance station location and ambulance response time.  In the literature review, four databases were used as EBSCOhost, Proquest, Science Direct, and SpringerLink and through access help from Brawijaya University library. PRISMA method was exerted in this research to select the articles and choose as many as 15 research articles that fulfill inclusion criteria. The response time of EMS was very influential for patient care and safety. The standby ambulance location can affect the officer's response time. The determination of the ambulance location for the EMS service provider was very significant to offer an optimal service.
Analysis of the Relationship between Snake Fruits Sidempuan (Salacca sumatrana Becc.) and Riring (Salacca zalacca var. amboinensis) using the Morphological Characterization Approach Matatula, Erfina Anace; Ashari, Sumeru; Soegianto, Andy
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.2

Abstract

Genetic relations estimation can be used for germplasm management activities, identifying cultivars, selecting parents for crossing, and reducing the number of individual samplings in a wide range of genetic diversity. The research's fundamental purpose is to analyze kinship relationships and study the genetic diversity between the snake fruit Sidempuan plant from South Tapanuli with snake fruit Riring plants from Ambon Island. Characterization research was conducted in Padang Sidempuan, South Tapanuli, and Riring Village, Ambon Island, using Radford characterization guidelines. The morphological characterization results showed that the Sidempuan and Riring bark's ecotypes were divided into 3 main clusters and had a kinship relationship with the value of genetic similarity of 85% -100%. Even though it has similarities, there were several distinguishing characters, namely the color of the upper surface of the leaf, the folding of the edges of the leaf blade, the taste of the flesh of the fruit, the height of the plant, and the category of snake fruit flowers. In a nutshell, although they looked similar, the two ecotypes were not identical.
Abundance and Diversity of Fruit Flies Species (Diptera: Tephritidae) in Bogor and Depok District Octavia, Evy; Tarno, Hagus; Himawan, Toto; Setiawan, Yogo
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.7

Abstract

Fruit flies (Diptera: Tephritidae) are important pests of horticultural crops. This study investigates the abundance and species richness of Bogor and Depok District as a center area horticulture commodity based on Cue Lure Trap. Each Cue Lure Trap was observed to record the abundance and species richness of fruit flies at seven days intervals from June until August 2021 in Bogor District (Babakan, Mekarsari and Parigi Mekar) and Depok District (Bedahan and Pancoran Mas), West Java, Indonesia. A total of 1,025 individuals were collected and consist of nine species of fruit fly i.e., Bactrocera albistrigata, B. carambolae, B. neocognata, B. verbascifoliae, Dacus conopsoides, D. longicornis, Zeugodacus calumniata, Z. caudatus, and Z. cucurbitae. The number of individuals of fruit flies was highest in the Bedahan, Depok District (F = 5.454, P = 0.002). Zeugodacus cucurbitae was the most abundant species (792 individuals), representing 77.27% of the specimens collected during the sampling period. The highest Shannon–Wiener diversity index of all fruit flies captured in this study was in Bedahan, Depok (1.11) and the lowest in Parigi Mekar, Bogor (0.23). A Bray–Curtis analysis showed that the species compositions of fruit flies on Bedahan and Parigi Mekar had a similarity of 70.1%.

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