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Research Journal of Life Science
Published by Universitas Brawijaya
ISSN : -     EISSN : 23559926     DOI : https://doi.org/10.21776/ub.rjls
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 202 Documents
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Analysis of Metabolit and Antibacterial Control of Mengkudu Leaf Extract (Morinda citrifolia) on the Bacteria of Aeromonas hydrophila Yora Utami Putri Pertiwi; Arief Prajitno; Mohamad Fadjar
Research Journal of Life Science Vol 6, No 3 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2019.006.03.3

Abstract

A. hydrophila is an anaerobic facultative bacterium, which is a bacterium that can develop in conditions with or without oxygen. Therefore, it is necessary to use alternative materials that can be used to eliminate A. hydrophila, one of which is to use herbal medicines. The purpose of this study was to determine the content of active compounds in mengkudu leaf extract (M. citrifolia) using the disc test method. To test the content of secondary metabolites, phytochemical testing and FTIR (Fourier Transform Infrared Spectroscopy) were carried out. The results obtained are 100 ppm which is the minimum concentration that can inhibit the growth of A. hydrophila. Phytochemical test results obtained crude extract of positive mengkudu leaves alkaloids, saponins, triterpemoid and flavonoids. And in the FTIR test the most dominant content of phenol compounds contained in Mengkudu leaf extract which has a very important function that can be used as an antibacterial in this study. Further research is expected to carry out more in-depth characterization testing of active compounds through several tests including using LCMS or GCMS to determine the active ingredient of mengkudu leaves.
Decreasing Family Anxiety Level of Acute Coroner Syndrome with Slow Deep Breathing Relaxation Zulkifli B Pomalango; Titin A Wihastuti; Yulian W Utami
Research Journal of Life Science Vol 7, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.01.1

Abstract

Acute Coronary Syndrome Patients can cause anxiety for the family. Anxiety of ACS family in Aloei Saboe hospital was found to be a family of moderate anxiety of 18 people (81.8%) and a severe anxiety of 4 people (18.2%). This study used the research design of One Group Pretest Posttest Design with 22 respondents. Respondents were given slow deep breathing exercises, given for 15 minutes. The bivariate analysis used is the Marginal Homogeneity Test. The results of the study were obtained before being given treatment, the family with moderate anxiety amounted to 18 people (81.8%) and a heavy anxiety of 4 people (18.2). After being given treatment there was a decrease in the level of anxiety, the family with moderate anxiety numbered 9 people (40.9) and mild anxiety about 13 people (59.1%). The results of the statistical test for marginal homogeneity obtained p values of 0.000 (p<0.05). There is a slow deep breathing effect on decreasing family anxiety level of acute coronary syndrome patients. Further research, slow deep breathing actions can be combined with health education in overcoming the anxiety of families who care for patients in hospitals.
Design and Effect of Escape Vent in a Trap on the Catch of Blue Swimming Crab (Portunus Pelagicus): A Preliminary Study Rahman, Muhammad Arif; Iranawati, Feni; Sambah, Abu Bakar
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.2

Abstract

Blue Swimming Crab (Portunus pelagicus, BSC) is one of the Indonesian important fisheries commodities often captured by traps. Under Decree No. 56/2016, the Ministry of Marine Affairs and Fisheries managed the BSC, with the standard size of BSC is >10cm of Carapace Width (CW) or >60 g of Weight (W), and non-berried or carried eggs for female. This study aims to determine BSC's crawling pattern toward the trap, design an escape vent on the trap, and assess catch of BSC using vented trap and non-vented trap. The video footage was used to examine crawling pattern of 30 BSCs on the laboratory. The study also includes field experiment by deploying twenty traps (10 vented traps and 10 non-vented traps) on the sea. The study obtained two crawling patterns of BSC, crawling forward and sideways with the most BSC crawled sideways. Design of escape vent was 4.6 cm length x 2.6 cm height. The BSCs captured in the vented traps have >10cm of CW, while 14% of BSCs in the non-vented traps have <10 cm of CW. The use of escape vent did not affect the catch (weight) of BSC in the trap.
Effect of Self-Directed Videos and Simulations on Nurse Skills in Advanced Cardiac Life Support: Comparison Study Jatim Sugiyanto; Karyono Mintaroem; Titin Andri Wihastuti
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.3

Abstract

Cardiac arrest is one of the highest causes of death in the world. This condition requires the handling of cardiac arrest based on the chain of survival concept. The chain of survival concept is carried out by nurses who have sufficient skills to handle cardiac arrest. Skills can be obtained either through education and training based on simulation or using technology such as self-directed videos. The purpose of this study was to identify the differences in self-direct video and simulations of nurse skills in advanced cardiac life support. This study used Quasi-Experimental Design by pretest-posttest with a control group approach. The 42 respondents were divided into two groups. Skills are measured before and after learning with each method. Data were analyzed using Wilcoxon and Mann-whitney tests. The results showed changes in skills before and after learning in the two methods, with each p-value = 0.000. There were no differences in skills changes before and after learning in both groups with a p-value = 0.437. Learning using self-directed videos and simulations can improve nurse skills in carrying out advanced cardiac life support.
The Effect of Moringa Oleifera Leaves Powder to Level of Serum Superoxide Dismutase (Sod), Lead (Pb), Zink (Zn)And Memory Function of Rat (Rattus Norvegicus) Wistar Strain Model of Autism That is Exposed by Pb Agustiana Dwi Indiah Ventiyaningsih; Hidayat Sujuti; Winda Nurtika; Septi Nur Rachmawati; Nurshalilah Nurshalilah; Raudhatul Jannah
Research Journal of Life Science Vol 7, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.01.6

Abstract

Lead exposure (Pb) may aggravate the decrease in serum SOD levels, memory function reduction and social interaction in autism. This study aims to determine the effect of Moringa oleifera on autism models of rat exposed to lead. The five groups of rat exposed to tin were studied: normal rat (C -), autistic rat (C +) and autism rats given 180 mg (T1), 360 mg (T2) or 720 mg (T3) oleifera leaves powder. Lead acetate is given through a filler tube (0.5 gr / kg) and powdered moringa leaves mixed with feed. The results showed that serum SOD levels were lower in autistic rat than normal rat and intake of Moringa olifeifera leaves powder increased serum SOD levels in autistic rats but was not seen in normal rat. The autistic rat had higher lead levels but lower zinc levels, which were not influenced by Moringa olifeira. The social interaction between rats is strongly influenced by unbidden lead with Moringa olifeira, both in normal rat and in autistic rat. Moringa oleifera will increase serum SOD levels in autistic rats but not at normal levels. Moringa olifeira does not protect significantly rat from lead causing less social interaction, nor does it lower serum Pb levels or increase Zn levels. Moringa leaves powder does not affect memory significantly.
Phenomenology Study : Family Experience in Elderly Care In Depok Jawa Barat Yati Sri Hayati; Asti Melani Astari
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.8

Abstract

The family has an important role in elderly care at home, where the majority of assistance and long-term care for the elderly is provided by the family. The nurse is responsible for assisting the family in caring for the elderly, so that in the end the family is able to provide elderly care independently at home. This study aims to explore family experiences in caring for the elderly at home. Researchers used qualitative research methods with a phenomenological approach. Data obtained through semi-structured interviews with 6 (six) participants. The research produced 5 themes: care for the elderly is a form of devotion to parents, care for the elderly requires physical and mental readiness, care for the elderly requires knowledge related to the elderly and how to care for the elderly, care for the elderly requires support from extended families, and feel the importance of supporting cadres and health workers. For conclusion, elderly families have a responsibility to meet the needs of the elderly, so that efforts are needed to improve the knowledge and skills of the family in order to properly care for and meet the needs of the elderly.
Risk Factors Related to Recurrence Rates of Acute Coronary Syndrome in Pakraman and Non-Pakraman Villages in Bali Province Ni Luh Sri Wahyuni; Tina Handayani Nasution; Edy Widjajanto
Research Journal of Life Science Vol 7, No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.02.3

Abstract

The prevalence of Acute Coronary Syndrome (ACS) is increasing in both developed and developing countries. Uncontrolled risk factors such as smoking, hypertension, and fewer activities are the main causes of ACS recurrence within 30 days after the acute presentation. This study aims to analyze risk factors related to the recurrence rate of ACS patients in Pakraman and non-Pakraman villages in Buleleng Regency, Bali Province. Observational analytics with Cross-Sectional Study are used in this study involving 130 patients within 1 month. Data is collected through two (2) stages using a questionnaire. The bivariate analysis shows that both smoking groups (p = 0.003 and p = 0.008) and hypertension groups (p = 0.02 and p = 0.01) are associated with ACS recurrence, while physical activity groups are not related (p = 0.455 and p = 0.565). Logistic regression results show that smoking (5.633) is highly associated with ACS recurrence in Pakraman villages, while hypertension (4.784) is highly associated with ACS recurrence in non-Pakraman villages. ACS handling is important in the acute period and in the long-term to prevent the risk of recurrence.
Growth of Beauveria bassiana combined with MIPC Insecticide and its efficacy to control the brown planthopper Nilaparvata lugens Aminudin Afandhi; Yayan Atma Kunjana; Fery Abdul Choliq; Amin Setyo Leksono
Research Journal of Life Science Vol 7, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.01.2

Abstract

The objectives of this study were to evaluate the efficacy of MIPC to control the brown planthopper Nilaparvata lugens, one of the most damaging pests of rice in the field and determine the effect of various of its doses on the growth of the fungus B. bassiana. The research was carried out in a rice field and in the Biological Agents Development Laboratory, Department of Pests and Plant Diseases, Faculty of Agriculture, Brawijaya University, Indonesia. The field efficacy test of the MIPC insecticide was conducted by applying 0.5-, 1.0-, 1.5-, and 2.0-kg/ha doses. The insecticidal compatibility test of MIPC with B. bassiana was conducted in 0.5-, 1.0-, 1.5-, and 2.0-g/L doses of MIPC. Application of MIPC insecticide at 0.5-, 1.0-, 1.5-, and 2.0-kg/ha doses had no significant difference in reducing N. lugens population in the vegetative phase (28 to 70 days after planting). The MIPC doses of 1.0, 1.5, and 2.0 g/L were highly toxic or incompatible with the growth of B. bassiana. Meanwhile, a sublethal concentration of MIPC insecticide of 0.5 g/L was non-toxic to B. bassiana. Therefore, this study recommends the use of 0.5 g/L MIPC combined with B. bassiana fungi.
Optimized Expression Condition of CIDRα-PfEMP1 Recombinant Protein Production in Escherichia coli BL21(DE3): A Step to Develop Malaria Vaccine Candidate Setyoadji, Winnie Almira; Sulistyaningsih, Erma; Kusuma, Irawan Fajar
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.3

Abstract

Malaria is still an essential epidemiological disease worldwide, including in Indonesia. Several approaches are performed to control the disease, as well as vaccine development. The Cysteine-rich interdomain region α of Plasmodium falciparum erythrocyte membrane protein 1 (CIDRα-PfEMP1) is a pivotal domain in the malaria pathogenesis make it a malaria vaccine candidate. The development of the malaria vaccine is performed using recombinant technology. Recombinant protein production is an important step. The study aimed to determine the optimized condition for CIDRα-PfEMP1 recombinant protein expression in Escherichia coli BL21(DE3) expression system. Serial IPTG concentrations from 0.05, 0.1, 0.3, and 0.5 mM and two different incubation periods of 4 h and 8 h were optimized. The recombinant protein expression was visualized in SDS-PAGE, measured using the Bradford protein assay, and calculated using software Image J. SDS-PAGE visualization showed a 27 kDa band expressed CIDRα-PfEMP1 recombinant protein. The optimized condition for CIDRα-PfEMP1 recombinant protein expression was at 0.03 mM IPTG concentration and 8 h incubation period.
Cytochrome Oxidase C Subunit I (COI) for Identification and Genetic Variation of Loaches (Nemacheilus fasciatus) Amalia Ayuk Riyadini; Maheno Sri Widodo; Mohamad Fadjar
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.4

Abstract

Nemacheilus fasciatus is an Indonesian freshwater fish species that can be found in river waters on Java. This fish has a morphological similarity between species. This study aims to identify species of samples found and know their genetic kinship. This study used the Cytochrome Oxidase subunit I (COI) gene as a molecular marker, and then the results were analyzed using MEGA X software. The genetic structure and phylogeny of N. fasciatus sequences were combined with outgroup species from GenBank and analyzed using Maximum Likehood (ML), Pairwise Genetic Distance and Bootstrapping Phylogeny Model of Kimura 2 Parameters. The results showed that the primary pairs of LCO1490 and HCO2198 used to amplify the sample N. fasciatus with COI as a marker. The nucleotide frequencies of these loaches are A=26.5%, T=23.8%, C=19.9% and G=29.9%. The estimated Transition/Transversion bias (R) is 0.60. The number of haplotype diversity (Hd) was 0.972, and nucleotide (Pi) diversity was 0.05115. The kinship of N. fasciatus compared to the outgroup is closer to N. pallidus than N. chrysolaimos. Research needs to be conducted with a larger sample size for the genetic diversity of N. fasciatus in Indonesia.

Page 13 of 21 | Total Record : 202

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