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Research Journal of Life Science
Published by Universitas Brawijaya
ISSN : -     EISSN : 23559926     DOI : https://doi.org/10.21776/ub.rjls
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 202 Documents
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Effect of Prenatal Yoga on Duration of the First Stage of Labor and Perineal Rupture in Primigravida Mothers Daniyati, Asri; Mawaddah, Shohipatul
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.5

Abstract

In primigravidas, the first stage of labor has a longer duration than multiparous. The first stage of labor in primiparous is around 13-14 hours, while in multiparous it is about 7 hours. Prolonged labor is one of the causes of maternal death. Yoga in pregnancy can maintain elasticity and strength of the ligaments of the pelvis, hips, and leg muscles, so that it can reduce pain during labor and provide space for the birth canal.  The comparative analytical research method used pre-experimental design with the one shot case study design. With a sample of 26 trimesters III primigravida pregnant women who were divided into 2 groups, namely n = 13 groups who did prenatal yoga, and n = 13 groups who did not do prenatal yoga. The analysis of this research was univariate and bivariate using the Mann Whitney test. Assessment is carried out during the delivery process. As a result, the intervention and control groups with active phase I labor duration obtained p = 0.000, and perineal rupture p = 0.000. There was a significant difference in the length of the first stage of labor and perineal rupture between the yoga group and the non-yoga group. Prenatal yoga affects the duration of the first stage of labor and perineal rupture.
The Role of Zinc Supplementation in The Lung Tissue Damage of Tuberculosis Rats Sukma Sukma Sahadewa
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.6

Abstract

Infection by Mycobacterium tuberculosis (Mtb) is a chronic contagious disease with a high mortality rate in various parts of the world, especially in developing countries with high poverty rates. The objective of the present study was to examine the effects of zinc supplementation on the lung tissue damage of the male tuberculosis rats. This study was conducted based on true experimental design, using a post-test control group design. Peribronchiolitis was found to have a higher rank in (K0, K1, K2) groups compared to the Mtb group treated with the addition of Zinc supplements (P1, P2, P3). Zinc supplementation in this study shows that there is a reduction in lung organ damage and having a positive impact on increasing the perivasculitis and granuloma. Zinc supplementation by its optimal dose of 50 mg/kg.bb/day given as additional nutrition in Mtb rats.
Phytochemical Analysis and in vitro Antibacterial Activities of Seagrass Enhalus acoroides against Staphylococcus aureus Desy Setyoningrum; Ade Yamindago; Syarifah Hikmah J. S; Maftuch Maftuch
Research Journal of Life Science Vol 7, No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.02.2

Abstract

Several studies of marine bioactive compounds have been carried out using seagrass. Enhalus acoroides is a type of seagrass that has bioactive compounds including alkaloids, flavonoids, tannins, steroids, and saponins that have potential as an antibacteria. The aim of study is to investigate the phytochemical compound contained in the crude extract of Enhalus acoroides and its antibacterial activities of Enhalus acoroides against Staphylococcus aureus. The seagrass was collected, washed, dried, grind and exposed to extraction by vacum rotary evaporator at temperature of 40°C and was analyzed for their phytoconstituents. Further, the crude extract was tested against pathogenic bacterial at different concentrations using disc diffusion method to determine the effect of bioactive compounds in E. acoroides to bacteria. In the present study, qualitative test of phytochemical from ethanol extract of Enhalus acoroides leaves contained phytochemical compound of alkaloids, flavonoids, tannins, steroids and saponins, while the extract of roots contained all of compounds except saponins. Based on research can be concluded, that Enhalus acoroides also has antibacterial activity against Staphylococcus aureus that showed by the presence of inhibition zone. The present finding suggests that the extract of seagrass Enhalus acoroides can be used an antibacterial agent from marine
The Biological Aspects Comparison of Nila Tilapia (Oreochromis Niloticus) on The Eutrophic and Oligotrophic Reservoir from Indonesia Arfiati, Diana; Saputri, Nadya Agustarina; Styaningrum, Nimas; Widyastami, Asthervina; Dina, Karina Farkha; Lailiyah, Shofiyatul; Pratiwi, Rizky Kusma; Inayah, Zakiyyah Nur
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.1

Abstract

Comparison of the length, weight and condition factor of Nila Tilapia (Oreochromis niloticus) was studied on oligotrophic waters (Wonorejo Reservoir) conducted from July to August 2018 and eutrophic waters (Selorejo Reservoir) on February to March 2019. All of the reservoir are in the East Java, Indonesia. The total sample from Wonorejo Reservoir about 79 fish from 3 times sampling (every two weeks), but the fish Sample from Selorejo Reservoir are 35 from 4 times sampling every week. The Nila Tilapia from Wonorejo Reservoir are 15.3 ± 2.5 cm (length) and 74.6 ± 38.6 grams (weight) but fish from Selorejo are 17.8 ± 2.2 cm average length and 114 ± 35 grams for the weight. The condition factor of Nila Tilapia in both reservoirs was relatively the same, with an average value was 1.01. The Nila Tilapia caught from Selorejo Reservoir (eutrophic) is relatively heavier and bigger than in the Wonorejo Reservoir (oligotrophic) even though the hook size is smaller. Fish from the Selorejo Reservoir (eutrophic) is easier to get food so that the average fish is larger.
Correlation Analysis of Knowledge and Education Against the Implementation of Basic Life Support (BHD) in Patients of Cardiac Arrest by Nurses in Emergency Installation and ICU of General Hospital of Dr. Soedarso Pontianak Maulidah Maulidah; Dhelya Widasmara; Titin Andri Wihastuti
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.2

Abstract

Cardiac arrest is a condition where the heart stopped abruptly, and this condition usually occurs in someone who has had heart disease or has never experienced it. In terms of clinical, someone who is experiencing a state of cardiac arrest found no signs of a palpable pulse and other signs of circulation. Efforts in terms of handling cases of the cardiac arrest itself need the role of nurse, one cardiac arrest treatment efforts by nurses are implementing Basic Life Support. The factors that affect the implementation of BHD itself is knowledge and education. The purpose of this research is analizing the correlation between knowledge and education on the implementation of BHD in cardiac arrest patients by nurses in the Emergency Installation and ICU of the General Hospital of Dr. Soedarso Pontianak. This study uses a cross-sectional approach with descriptive corelational type. The population in this study are 56 nurses. Sampling technique using total sampling. The result of this study, based on two variables: the knowledge and education have the same p-value that is p=0.000 or α <0.05. It means that there is a correlation between knowledge and education on the implementation of BHD in patients with cardiac arrest in General Hospital of Dr. Soedarso Pontianak.
The Potency of UB Forest Bacteria as Chitinolytic Bacteria to Inhibit Anthracnose Disease on Cayenne Pepper Nava Karina; Achmad Roekhan; Cindy Diah Ayu Fitriana; Luqman Qurata Aini
Research Journal of Life Science Vol 7, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.01.5

Abstract

Anthracnose disease is a major disease in cayenne pepper triggered by fungal pathogen Colletotrichum capsici which can cause yield losses of up to 90%. Control of disease generally utilizes synthetic fungicides, but it damages the environment and human health. Hence, alternative controls that are ecofriendly are required. One of the attempts is to make use of chitinolytic bacteria's potential. This study aimed to screen UB Forest chitinolytic bacteria and to determine their potential in suppressing anthracnose in cayenne pepper. Stages of research include a screening of 78 UB Forest bacteria as chitinolytic bacteria, selection of chitinolytic bacteria that are antagonistic against C. capsici, inhibition test of chitinase crude extracts against C. capsici growth, controlling anthracnose disease in cayenne using chitinolytic bacteria, and molecular identification. Based on the results of the selection, there were 76 isolates of UB Forest bacteria classified as chitinolytic bacteria, 74 bacteria antagonistic against C. capsici. Chitinase crude extracts significantly affected C. capsici growth inhibition compared to fungicide treatment. The application of Pseudomonas aeruginosa (UB 11) and Bacillus cereus (UB 12) can control anthracnose disease in cayenne pepper with the intensity of 41.67% and 38.89% respectively with the efficacy level of 58.33% and 61.11%.
Potential Analysis of Lemna sp. Extract as Immunostimulant to Increase Non-Specific Immune Response of Tilapia (Oreochromis niloticus) against Aeromonas hydrophila Armando, Eric; Lestiyani, Ayu; Islamy, R. Adharyan
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.6

Abstract

Lemna sp. is known to have several bioactive compounds and polysaccharide macromolecules that can function as immunomodulators to affect non-specific immune responses to increase the body's resistance to pathogens. This study aims to determine the potential of catfish eye extract as an immunostimulant by observing non-specific tilapia immune parameters. The extraction method used was 96% ethanol maceration for 2 days with a ratio of 1: 4. The experimental design used a Completely Randomized Design with 5 treatments (doses 0.2, 0.4, 0.6 mg/kg, control + and control -) and 3 replications. The non-specific parameters of immunity observed included total plasma protein (Bradford method), superoxide dismutase and lysozyme activity. The data obtained will be analyzed using ANOVA, if there is a significant difference, it will be further tested with Duncan Multiple. Range Test (DMRT). The results showed that the highest total plasma protein was found in treatment C (giving an extract of 0.3 mg/kg body weight) with an average total plasma protein after 12 days of maintenance of 4.99 g / dL. The extract dose of 0.3 mg/body weight showed a rapid decrease in SOD and increase Lysozyme activity.
The Effect of Pregelatinization with Heat and Moisture Treatment on Physicochemical and Pasting Characteristics of Red Glutinous Rice Flour Jhauharotul Muchlisyiyah; Hera Sisca Prasmita; Teti Estiasih; Ratna Palupi Nurfatimah
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.7

Abstract

Red glutinous rice is native from Pacitan and is still limited to local region consumption. The color of the rice flour showed that it has bioactive content, which is the potential to wider the use. The effect of pregelatinization using heat and moisture treatment by boiling on physicochemical (starch, amylose, anthocyanin, gel consistency, Lab color, SEM figure) and pasting properties of red glutinous rice flour was determined. The time and temperature of pregelatinization were 5, 10, and 15 minutes and 50°C, 60°C, and 60°C. The experiment was done with a completely randomized design with three replications. Starch content range to 62-77%, amylose content 3.29-5.75%, anthocyanin 2.64-6.71 mg/100g, Moisture 6.16-7.45, L 72.5-73.95, redness 5.11-6.00, yellowness 8.87-9.62, and gel consistency 9.7-12 cm. the increase of time and temperature of pregelatinization lead to decrease of amylose, starch, and anthocyanin content, and viscosity. After pregelatinization, the setback viscosity was lower it means that the rate of retrogradation is lower than native starch. SEM figure also showed that pregelatinization using heat and moisture treatment did not destroy the starch granules.
Characteristics of Tuna Viscera (Thunnus sp.) Hydrolysate Protein Fermented by Bacillus licheniformis Galih Nugroho; Arning Wilujeng Ekawati; Hartati Kartikaningsih
Research Journal of Life Science Vol 7, No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.02.4

Abstract

This study aims to analyze the nutritional composition of the degree of hydrolysis and amino acids in the internal organ waste of tuna and the protein hydrolyzate of tuna's internal organs after fermentation using the Bacillus licheniformis bacteria. The analysis showed that the protein content of tuna offal was 53.52%, and after fermentation by Bacillus licheniformis, bacteria were able to increase protein levels from 56.04. The degree of hydrolysis of protein (DH) showed an increase of 13.24% in tuna offal to 22.28% of protein hydrolyzates are fermented tuna innards. The total essential amino acids and non-essential amino acids in the fermented tuna innards' protein hydrolyzates increased during the fermentation process. The highest levels of essential amino acids were found in arginine as high as 3.632965 at the 96th hour, and the lowest histidine was 1.082602. In contrast, for the highest non-essential amino acids, there was glycine at 8.52223, and the lowest for tyrosine was 1.272592.
Morphological Characteristics of Bioactive Compounds on Api-Api Mangrove Leaves Extract (Avicennia marina) Based on Leaves Age Muhamad Zaky Zamani; Arief Prajitno; Mohamad Fadjar
Research Journal of Life Science Vol 6, No 3 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2019.006.03.4

Abstract

Api-api leaves A. marina has a variety of active compounds that have been confirmed to too wordy be used as antibacterial agents, where harvesting of mangrove leaves is carried out at the location of Betoyoguci Gresik and differentiated by age, this age differentiation is based on morphological characteristics because it has color differences significant. Where age is one of the factors that affect the level of active compounds found in leaves. Extraction results of mangrove api-api gave different yield levels in each age level. The order of yields from the largest to the smallest is the old leaves 3%, fall 2.86% and young leaves 2.04%. Whereas based on phytochemical test the best extracts were obtained, namely old leaves, fall leaves and young leaves, FT-IR results with 10 detected absorption files which indicate the presence of functional group characteristics that can be used as an illustration of the presence of antibacterial compounds. In the LC-MS test with identification of several molecular weights which indicated a signal derivative of flavonoid compounds in the form of dihydroquercetin, quercetin-3-O-β-D-xylopyranoside, quercetin-3-O-β-D-galactopyranoside, isohametin-7-O-pentoside and routine, and derivatives of detected alkaloid compounds in the form of berberine and papaverine.

Page 15 of 21 | Total Record : 202

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