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Research Journal of Life Science
Published by Universitas Brawijaya
ISSN : -     EISSN : 23559926     DOI : https://doi.org/10.21776/ub.rjls
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 202 Documents
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Lubricant Oil Bioremediation by Rhodococcus erythropolis Bacteria and Indigenous Bacteria Isolated from Water Contaminated with Lubricant Oil Frentina Murti Sujadi; Yahya Yahya; Andi Kurniawan; Abd. Aziz Amin
Research Journal of Life Science Vol 7, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.01.7

Abstract

The bioremediation system can be improved by using specific bacterial potential as oil-degrading bacteria which microorganisms can use hydrocarbons as a carbon source for their metabolic processes. The aim of this study is to identify R. erythropolis on degradation oil contamination and to obtain indigenous bacteria as new agent bacteria on bioremediation of oil contamination. The polluted water samples from used oil were taken from PPN Prigi, Trenggalek, East Java, Indonesia. The parameters considered were the detection and characterization of indigenous bacteria that degraded used oil. The density of bacteria was analyzed in the interval time of days 0, 2, 4, 6 and 7 and TPH was analyzed at final incubation. The results of this study indicate that the effectiveness of reducing oil concentration was used in testing the potential of bacteria from the highest was Pseudomonas aeruginosa which as indigenous bacteria isolated from water contaminated sites with application cell rate 1×108 cells/ml. It reduced of oil concentration up to 53%, and 1×106 cells/ml reduced oil concentration up to 47%. While, R. erythropolis with application cell rate 1×108 cells/ml reduced 47%. This result was found that Pseudomonas aeruginosa was effectively removed of oil concentration.
Density and Biomass of Seagrass Beds and Its Threats on Lamongan Regency Dewi, Citra Satrya Utama; Yona, Defri; Samuel, Pratama Diffi; Maulidiyah, Rizqi Aimmatul; Syahrir, Ahdiya; Putri, Yandini Eka; Rakhmawan, Hilal; Fikri, Maulana
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.9

Abstract

This study aims to determine the density of seagrass species, seagrass biomass, and threats faced by seagrass communities in the Coastal District of Lamongan. The study was conducted in August to November 2019 in three coastal villages of Lamongan Regency, namely Tunggul Village (Station 1), Kranji (Station 2), and Banjarwati (Station 3). The stages of the study consisted of: preparation, data collection on density, threat data and seagrass stand samples, sample processing, and data analysis. The results showed that the total density of seagrass species at Stations 1, 2 and 3 was 160 stands / m2; 172 stands / m2; and 185 stands / m2. Furthermore, the total biomass of seagrass at Stations 1, 2 and 3 was 4633 gbk / m2, 2136 gbk / m2, and 9234 gbk / m2. In addition to seagrass species, seagrass density values also affect seagrass biomass. As development progresses in Lamongan Regency, it is known that the biggest threat of seagrass communities in the study site is human activity (anthropogenic), among others: anchor ships when anchored and the use of coastal areas as tourist areas, which often cleans up seagrass communities because they are deemed unfit to be enjoyed tourism sector.
Profile of Amino Acid and Fatty Acid from Different Type of Worm as Feed of Broodstock Candidates for Domesticated Uceng (Nemacheilus fasciatus) Sholikah, Lik Anatus; Soeprijanto, Agoes; Kilawati, Yuni
Research Journal of Life Science Vol 7, No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.02.5

Abstract

Uceng fish (Nemacheilus fasciatus) is a fish that lives wild on the river. All this time, the people have only relied on fishing from nature to consume, but the high market demand for fish will increase river fishing activities in excess. One of the steps to suppress the decline in fish populations is domestication because currently, the fish is not yet cultivated. The domestication process by feeding worms containing amino acids and fatty acids accelerates broodstock candidates' gonadal development. This research aims to determine the type of worms suitable as feed for the growth and gonadal development of broodstock candidates. The method that used in this research is Completely Randomized Design (CRD) with 3 treatments and 3 repetitions. Based on the analysis of amino acids and fatty acids in each type of worm, the best results were obtained on Lumbricus rubellus with an amino acid content of 50.91 gr/100gr and fatty acid 292.27%.
Analysis of Platelet Function from Thromboelastography Examination in Patients with Single and Multiple Antiplatelet Therapy after Percutaneous Coronary Intervention at Dr. Saiful Anwar Malang Sasmojo Widito; Dadang Hendrawan; Dedy Irawan
Research Journal of Life Science Vol 7, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.01.3

Abstract

Each year, approximately 3 million people with coronary heart disease worldwide undergo percutaneous coronary intervention (PCI). Dual antiplatelet therapy (DAPT) with aspirin and P2Y12 inhibitors became the primary therapy for 6-12 months after PCI. DAPT can be continued > 12 months at a high risk of thrombosis. About 9-10% of patients with dual antiplatelet therapy still experience ischemia. The platelet function examination by thromboelastography (TEG). This research is an analytic observational study using a cross-sectional method. This study was conducted in Saiful Anwar General Hospital. Patients were divided into two groups: (1) on-single antiplatelet therapy; (2) on-dual antiplatelet therapy. The outcome measured result of the platelet function test was divided into standard, low platelet function, and platelet hypercoagulability. An analysis of the differences between single or multiple antiplatelet administration and the platelet function results was performed. There were 52 research subjects, each group of single and multiple antiplatelet therapies as many as 26 people, most of the subjects were male (82.6%) with a mean age of 57. The results of this study showed that there was no significant difference in the results of platelet function examinations between single and multiple antiplatelet therapies after 12 months of dual antiplatelet therapy
The Potency of UB Forest Chitinolytic Bacteria to Promote Plant Growth and Inhibit Damping off Disease on Soybean Roekhan, Achmad; Dayanti, Ayu Ike; Oktaviani, Rahmania; Dewi, Fibrianti Shinta; Anastasya, Nabilla Alya; Aini, Luqman Qurata
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.4

Abstract

Damping off disease in soybean plants is caused by the fungal Rhizoctonia solani. The damping off disease causes a yield loss of up to 85-100%. The purpose of this study was determining the ability chitinolytic bacteria consortium of UB Forest's in suppressing damping off disease in vitro and in vivo as well as its potential to stimulate the growth of soybean plants. The research stages included isolation of the pathogenic fungus R. solani and the pathogenicity test. Rejuvenation chitinolytic bacterial isolates of UB Forest, test chitinolytic bacteria antagonist of UB Forest against R. solani fungus, test of Plant Growth Promoting (PGP) activities, synergy test of selected chitinolytic bacterial isolates, in vitro test for the inhibition of chitinase crude extract against R. solani, and inhibition test of chitinolytic bacteria consortium against damping off disease. The selected chitinolytic bacteria were code bacteria UB12, UB19, and UB52 with plant growth promoting activities with inhibition percentage of the pathogen R. solani of 73.9%, 67.4%, and 71.7%. The best chitinolytic bacterial isolates were the genus Bacillus sp. and Pseudomonas sp. The inhibition test of chitinase crude extract showed an inhibition percentage of 25-55%.
Potential the Black Soldier Fly (Hermetia illucens) in Feed Formulation for Growth of Common Carp (Cyprinus carpio L.) Ayu Azkiyah Azizah; Arning Wilujeng Ekawati; Happy Nursyam
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.5

Abstract

The utilization of maggot as larvae from black soldier fly is an alternative substitution of fish meal as the primary protein source in fish feed. Maggot is cultivated in 3 different cultivation, the medium used is organic waste, tofu waste, and wheat pollard. The method that used in this research is Completely Randomized Design (CRD) with 3 treatments and 3 replication. This study aims to determine the effect of various types of maggot growth media, 1) with maggot biomass parameters, containers, and growing media conditions maggot, 2) then, exact proximate composition, total amino acids content of the prepupae samples were determined. Based on the results of the analysis, each dose of treatment (media) has an influence on the different maggot biomass. The best media treatment results are pollard flour. The proximate analysis showed that BSF larvae had a protein content of 50,88% and also fat content of 15%.
Consortia of Indigenous Rhizobacteria to Control Bacterial Wilt in Ginger Naziha Diyanatur Rosiyah; Ayu Wike Widiasari; Achmad Roekhan; Restu Rizkyta Kusuma
Research Journal of Life Science Vol 7, No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.02.1

Abstract

Ginger (Zingiber officinale Rosc.) is a type of rhizome plant that has high potential to be developed in Indonesia as a medicinal plant or spice. Inhibiting factors in ginger production is Ralstonia solanacearum which causes bacterial wilt disease. Rhizobacteria is a potential biological agent to control the disease. It is necessary to review from various study on rhizobacteria both the use of antagonistic bacteria singly or in a consortia. Aimed to analyze various sources of scientific literature regarding the potential of antagonistic bacteria singly or consortia compared to synthetic bactericides in controlling of R. solanacearum. The results showed that three recommended antagonist bacteria were found, namely Pseudomonas fluorescens, Bacillus subtilis, and Bacillus thuringiensis. which has the potential to control bacterial wilt disease. The rhizobacterial consortia are more effective as a sustainable, safe and environmentally friendly control on bacterial wilt disease with a percentage of disease incidence 10%. In addition, the rhizobacterial consortia are three and six times more effective than the single antagonistic bacteria and bactericides respectively.
Eating Behavior of Imago Aulacophora similis Oliver on Cucumber (Cucumis sativus L.) with Treatment Plant Growth Promoting Rhizobacteria (PGPR) Dwi Wulandari; Luqman Qurata Aini; Hagus Tarno
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.10

Abstract

The cucumber harvests failure occurred mainly due to the destruction of crops by the leaf-eating beetle A. similis Oliver. Many ways are taken especially pesticides that are synthesized widely because it is considered the fastest and most potent to overcome pest disorders. Based on, one of the control techniques that can be used is PGPR, which is a group of bacteria that can be utilized to strengthen plants against pest attacks and plant diseases, so research needs to be done by using this technique to change the feeding behavior of the A. similis Oliver beetle on cucumber plants. The research was conducted at the Green House Department of Pest and Plant Diseases University of Brawijaya Malang in September 2018 to March 2019. It was conducted using Randomized Block Design (RBD) 5 treatment, i.e., PGPR, urea fertilizer and control (without treatment). Based on the results of the study shows that: Application of PGPR on cucumber plant can reduce damage on either leaves or flower due to the eating behavior of imago A. similis Oliver and Application of PGPR can increase the cucumber plant growth during the eating behavior of imago A. similis Oliver.
Effect of Centella asiatica Extract on Locomotor Activity and Hsp60 Expression in Zebrafish models of Parkinsons Rodhiyan Rakhmatiar; Mulyohadi Ali; M. Dalhar
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.1

Abstract

Parkinson's disease characterized by a decrease in motor activity is a progressive neurodegenerative disorder caused by the degeneration of dopaminergic neurons. Centella asiatica is suspected of having a neuroprotectant effect and is not yet known how Centella asiatica role in the prevention of Parkinson's disease. The study was conducted to prove the effect of Centella asiatica extract on the expression of Hsp60 and locomotor activity zebrafish models of Parkinson's with rotenone exposure. The study was conducted using 25 zebrafish divided into various groups. Centella asiatica extract and rotenone exposure have given for 28 days, observed locomotor activity on days 0, 7, 14, 21 and 28. The expression of Hsp60 measured using immunohistochemical techniques. There is a significant difference between locomotor activity at various doses of Centella asiatica (p<0.05) with a very strong correlation (r=0.929; p<0.01) where the higher doses of Centella asiatica, the higher locomotor activity. Found a significant difference between the reduced expression of Hsp60 to various Centella asiatica dose group (p<0.05) but no correlation between the expression of Hsp60 with Centella asiatica dose groups and locomotor activity. Centella asiatica extract is able to increase locomotor activity and decrease the expression of Hsp60 in zebrafish models of Parkinson's.
Plankton Community Structure as Bioindicator Trophic Status of Jatigede Reservoir Waters Rosadi Rosadi; Muhammad Musa; Tri Djoko Lelono
Research Journal of Life Science Vol 7, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.01.4

Abstract

Jatigede Reservoir in Sumedang Regency is a land mass planning designed as a multi-function reservoir. The main water source for this reservoir is from Cimanuk River, which flows through Garut Regency, and has many industrial activities around the river flow. This research was conducted to assess the trophic status of water pollution in Jatigede Reservoir by utilizing plankton as a bioindicator agent. Samples were collected from 9 observation stations from November 2018 until January 2019. The results showed that 26 species of phytoplankton from 7 divisions including Dinophyta, Cyanophyta, Chlorophyta, Chrysophyta, Euglenophyta, Bacillariophyta, and Charophyta was found in Jatigede Reservoir at about 461 ind/m3. Zooplankton abundance of 6 species from 2 divisions of Rotifera and Copepoda at 2 ind/m3. The average phytoplankton diversity index was 0.93 and zooplankton diversity index was 0.23. The average phytoplankton evenness index was 0.44 and zooplankton evenness index was 0.24. The average dominance of phytoplankton was 0.58 and dominance of zooplankton was 0.25. Based on the plankton community structure, the trophic status of Jatigede Reservoir was classified to moderate polluted (eutroph) to heavily pollutants (hypereutroph). The dominant species was Perinidium sp from Dinophyta division.

Page 14 of 21 | Total Record : 202

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