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Articles 200 Documents

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Estimation of Structures Under Ancient Archaeological Sites Site of Jabung Temple, Probolinggo, Indonesia Using Geolistrik Resistivity Method Kosmas Isdarmadi; Adi Susilo; Sunaryo Sunaryo
Natural B, Journal of Health and Environmental Sciences Vol 2, No 1 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.natural-b.2013.002.01.8

The sounding of geoelectrical resistivity distribution using dipole-dipole configuration around the Jabung temple site located in the Jabung Candi village, Paiton, Probolinggo District, East Java province, Indonesia has been done by using Resistivitymeter OYO MacOhm. The aim of the research was to determine the distribution and depth of rock below the Jabung temple area also as an information for investigation and discovery efforts of ancient objects which are still burried. Aquisition are taken by ten lines and ±20.042 m2 of coverage area. Line 1, 2, 3, 4, 5, 6 located around in Menara Sudut temple at length of measurement line12 m and at a distance of electrode spacing are 1 m, where lines 1 and 4 located in outcrop of Menara Sudut tample. While lines 7, 8, 9, and 10 located in all area of Jabung temple. Lines 7 and 8 have length of measurement lines 150 m with spacing 10 m, while lines 9 and 10 have length of measurement lines 200 m with spacing 4 m, where line 8 located pass to main Jabung tample. The processing of data was using RES2DINV software. Resistivity values that are used to determine the temple rock is the resistivity value of sandy clay. Base on geoelectrical investigation and interpretation shows the sandy clay as temple rock with resistivity value 33 Ω.m to 92.6 Ω.m on average depth of 1.79 m and the others remain of tample rock around the main Jabung tample. This research shows geoelectrical method is good idea to subsurface sounding especially for archeology case, it caused this method is simple and more cheap than ekskavation method.

Determination of Seismicity with Grid Searching Method (Case Study of Gempabumi Pulung, Ponorogo Regency February 2011) Petrus Demon Sili; Adi Susilo; Sukir Maryanto
Natural B, Journal of Health and Environmental Sciences Vol 2, No 1 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Pulung Region, Ponorogo major city, Indonesia is one of the area in Indonesia which is prone of the local earthquake threat. Since 1963, there was about 7 big earthquakes, where 1 earthquake was destroyed earthquake and 2 events occured at the Pulung region. The two those earthquakes occured at Pulung were in 1993 and 1999, with the magnitude was 3.7 SR and 5.6 SR respectively. The last event was on February 2011, therefore it’s necessary to study and determine the seismicity. In addition the relocation analysis using Grid Searching seismicity showed that the distribution of earthquakes shifted to the East direction, with the coordinat of from 7.8122º to 7153º SL and 111.6360o to 111.7303° EL, the depth of earthquakes varies between 0.532 km and 7250 km, and the magnitude was between 1.4 and 2.0 SR. It is hoped that this result can be used for the govermnment of Ponorogo to mitigate of earthquake impact.

Effect of Metal Ions on Pectinase Activity from Aspergillus niger on Purifying Guava Fruit Dian Puspita Anggraini; Anna Roosdiana; Sasangka Prasetyawan; Diah Mardiana
Natural B, Journal of Health and Environmental Sciences Vol 2, No 1 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Pectinase can be used on various fruit juices clarification; however, the activities can be affected by their metal ions content. The purpose of this study was to determine the type of metal ions inhibition i.e. Zn2+; Mg2+ and Ca2+ on the pectinase activity of Aspergillus niger, and its potential application in guava juice clarification. Activity assays performed at pH 5, temperature 50°C during 55minutes and each ion concentration were 2-10 mM. Galacturonic acid concentration, as the product of pectin substrate hydrolysis, used as the basis for determining its activity and analyzed by visible spectrophotometry. The results showed that the ions concentration of Zn2+ and Mg2+ more than 4 mM were inhibitor for pectinase activity while Ca2+ ion concentration was 6mM. KI values for the metal ions Zn2+; Mg2+ and Ca2+ at concentrations of 6mM were 176.99; 326.09 and 280.37 mM respectively. Pectinase from Aspergillus niger were able to reduce 18% of pectin of guava juice.

BSA Immobilization on QCM Sensor with Hydrophobic-Hydrophilic Properness Modification of Polystyrene Surface Using Ultraviolet (UV) Radiation Fenny Irawati; Setyawan P. Sakti; Unggul P Juswono; Aulanni’am Aulanni’am
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Research has been done to improve the optimization of the BSA (Bovine Serum Albumin) immobilization process on QCM sensor by modifying the nature of the hydrophobic-hydrophilic polystyrene surfaces using ultraviolet radiation (UV). The silver electrode of QCM sensor coated with a solution of polystyrene using spin coating technique. Sensor with polystyrene coating was heated using an oven until it reaches a temperature of 200 °C and then irradiated with ultraviolet light. A 30 µL of distilled water was dropped on the sensor surface and the formed contact angle was observed. The observation of the contact angle showed that there was a change in the nature hydrophobicity of sensor surface irradiated with UV. BSA immobilization was done by injecting a certain amount of BSA concentration in PBS solution on top of the sensor. We found that best surface for immobilization of BSA is one which is without UV irradiation. This means that the immobilization is mainly caused by hydrophobic force.

Variant Clustering Analysis Amorphophallus muelleri Blume Found in East Java Based on Molecular Marking CslA Mannan Synthase Encoder with PCR-RFLP Technique Novie Ary Priyanti; Arik Arubil; Laras Estri Arumningtyas; Rodiyati Azrianingsih
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Amorphophallus muelleri Blume is a ubiquotus plant found all over Indonesia and has the potential to be food substitute due to its high glucose content. This research was conducted to investigate the porang’s clustering based on CslA gene glucose coding by using PCR-RFLP technique. Porangs used as the subjet of this study were obtained from 4 different locations in East Java, wich are : (1) Nganjuk, (2) Madiun, (3) Blitar and (4) Jember, analysed by morphological characteristic and molecular traits. Morphological analysis showed that porang derived based on their stalk pattern. BlitarI, JemberI, MadiunI variant have large prismatic stalk patterns, Jember1 and Nganjuk2 have a tight prismatic stalk pattern. Glucomannan content analysis showed that highest content is from Blitar2 5,469% and the lowest is Madiun2 0,2313%. The amplification of CslA gene sequence using primary AkCslA680F and AkCslA1288R showed that CslA gene sequence was amplified on 200 bp. The cutting by using HaeIII restrictive enzyme produced two monomorphic band on 1000 bp and 2000 bp, and 8 polymorphic band on 100 bp-400 bp. The result of this analysis showed that porang had high diversity. The analysis of among variants clustering based on their molecules data showed that grouping fell into 3 clusters, which were: cluster I (JemberI, BlitarI and MadiunI variants), cluster II (Jember2, Blitar2 and Nganjuk2 variants) and cluster III (Blitar2 variant). The shortest distance was found between JemberI and BlitarI variants, while the furthest was found in Blitar2 variants.

Temperature Effect of Extraction on Physicalochemical Properties Glukomanan Origin Amorphophallus muelleri Blume Nunung Harijati; Serafinah Indriyani; Retno Mastuti
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

This study had aims to find the optimum temperature for the extraction of the glucomannan with good physicochemical characters . The method that used for extraction was the stirring method according varies temperatures (35-55-75oC), followed by centrifugation with speed 1500 rpm for 30 minutes at temperatures 25°C. Glucomannan was coagulated using 95% isopropyl alcohol. Parameters that observed included glucomannan yield, ash and water content, flour color, lightness of glukomanan as well as calcium oxalate contents. The results showed that glucomannan extraction at 55°C gave optimal results (63.1% per dry weigh of tuber), followed by 52.2% and 39.91% for extracted temperature 35°C and 75°C respectively. There was no different significantly in color of glucomannan flour in between temperature which had L value 25.17; 27.23 and 26.37 correspond with extraction temperature 35°C, 55 °C, and 75°C. The calcium oxalate content of all treatment was 0.03-0,11% . The treatments also gave 1.17-2.00% ash content, 4.6-5.53% water content and 49.5-2654.5 Cp of viscosity.

Effect of Redox Time on Carbon Based Purity Made from Coconut Shell Simple Carbonization Zain Asrori; Lailatin Nuriyah; Djoko H Santjojo; Masruroh Masruroh
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Purification of carbon from coconut shell has been done by redox reaction method. Carbon was produced with simple heating carbonization method at 700 °C. Oxygen reduction process from the carbon was carried at temperature of 350 °C for 200 mL/min and 300mL/min with the H2 flow rate for 60 min, 120 min, and 180 min. Synthesis of carbon through simple heating carbonization method obtaining (93.84 ± 2.21) % of carbon content. Redox time optimization method successfully increased levels of carbon purity up (97.87 ± 1.47) %. The increase in carbon content was caused by the loss of a functional group with carbon-oxygen bond (C-O) and sulfur (S-O), as evidenced from FTIR test results.

Determination of Internal Structure of Semeru Volcano Based on Seismic Attenuation Imagery Hena Dian Ayu; Adi Susilo; Sukir Maryanto; Muhamad Hendrasto
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Characteristic of medium could be defined by physical parameters such as seismic velocity and attenuation. Amplitude, hyocentral distance, frequency and attenuation coefficient is required to determine the Q-factor value or attenuation in effort on imaging the internal structure. In this study, we used volcanic earthquakes data of Semeru Volcano recorded 2009 that consist 54 seismogram (24 A-type volcanic earthquakes and 24 explosion volcanic earthquakes. The Q-factor value of A-type volcanic earthquakes is 4 - 392 or about 78,87 and the Q-factor value of explosion volcanic earthquakes is 4 - 288 or about 51,39. In term of the image quality, it can be seen that the attenuation on Jongring Seloko active caldera and under Jongring Seloko have low attenuation value or high Q-factor value thas was called the zone beneath. The zone beneath is interpreted to associated with hot material that may indicate the magma chamber that concentrated in the depth interval of 1-1,8 km from the top of Semeru Volcano. The zone beneath have structural trends on West-East-Southeastern-South sector out the volcano. The internal structure of Semeru Volcano is predominance of sedimentary rocks, sandstone and rocks with gas in pore space. Although in some deep layer have internal structure as metamorphic rocks and igneous rock.

Study of Antibodies Expression with Specific Peptide Stimulation Specimens from gp350 / 220 (EBV) in Cell B Bambang Pristiwanto; Muhaimin Rifa’i
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Nasopharyngeal Carcinoma (NPC) is caused by Eipstein Barr Virus (EBV) which attack nasopharyngeal epithelium. EBV uses protein gp350/220 in the infection mechanism in nasopharyngeal epithelial surface receptor, CR2. The purpose of this study was to test the vaccine candidate at in vivo method. Mus musculus(Balb/C) mice were used as animal model. In this experiment mice pups in the age of 2 weeks were used. Injection using a specific peptide sequences from EBV showed higher expression of IgM (2.01%) compared to control (1.02%). Injection of this peptide to mice pups also increase the expression of IgM. After injection with this peptide, IgD also increase (75.74) compare to control (65.59%) without manipulation. In contrast, injection of this peptide decreases the expression of IgA antibodies (0.57%) significantly compare to control (1.93%).

Effect of Reduction Reaction Temperature on Carbon Purification based on Coconut Shell Marsi Bani; Djoko H Santjojo; Masruroh Masruroh
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

The purification carbon from coconut shell through carbonization with pyrolysis method at temperature 600 °C for 1 hour has been done. Carbon is refined in various temperature such as 150 oC, 200 °C, 250 °C, 300 oC, and 350 °C. The flow rate of carbon refining is 200 mL/minute for 1 hour using a reduction reaction method with hydrogen as the reducer to reduce oxygen content in the carbon. The result show indicates that the maximum carbon content 96,53 % at 350 °C. The increasing carbon content during the reduction process because hydrogen attaches oxygen to establish H2O bond and it is proved by the result of FTIR test.

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