cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Muchtaridi
Contact Email
ijpsteditor@gmail.com
Phone
-
Journal Mail Official
ijpsteditor@gmail.com
Editorial Address
-
Location
Kota bandung,
Jawa barat
INDONESIA
Indonesian Journal of Pharmaceutical Science and Technology
Published by Universitas Padjadjaran
ISSN : 23561971     EISSN : 2406856X     DOI : -
Core Subject : Health, Science,
Computer Science & IT Medicine & Pharmacology
Jurnal Sains dan Teknologi Farmasi Indonesia (IJPST) adalah publikasi ilmiah pada seluruh aspek Sains dan Teknologi Farmasi. Jurnal ini diterbitkan 3 kali setahun untuk menyediakan forum bagi apoteker, dan profesional kesehatan lainnya untuk berbagi praktik terbaik, meningkatkan jaringan kerja dan pendekatan yang lebih kolaboratif dalam Sains dan Teknologi Farmasi.
Arjuna Subject : -
Articles 511 Documents
 26   27   28   29   30 
Design and Cloning of Gene Encoding SLPI C-Terminal Domain in Escherichia coli TOP10 Evi Umayah Ulfa; Ni Nyoman Tri Puspaningsih
Indonesian Journal of Pharmaceutical Science and Technology Vol 9, No. 3, 2022
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v9i3.36918

Abstract

Elevated levels of neutrophil elastase in CPOD (Chronic Obstructive Pulmonary Disease) airways are regarded as the main trigger of lung destruction and inflammation. SLPI (Secretory leukocyte protease inhibitor), an inhibitor protease, represents an attractive candidate for treatment in chronic lung diseases due to proteases excess. The antiprotease active site of SLPI has been located on the C-terminal domain. This study aimed to design and clone the gene encoding the C-terminal domain of SLPI (SLPIc). The gene encoding SLPIc was optimized and predicted solubility using OptimumGene™ and SoDoPe software. The nucleotide sequence of the optimized SLPIc was synthesized, inserted into the pGEX 4T-2 vector commercially by Genscript, and transformed into the Escherichia coli TOP10. The pGEX 4T-2 vector contains a glutathione S transferase (GST) gene located before the MCS to generate a recombinant protein for fusion with GST. For purification purposes, the His-tag synthesized together with SLPIc. The optimized SLPIc nucleotide sequence gave a CAI value of 0.81, GC content 52.31, and a CFD of 2%. The solubility probability of SLPI fused with GST increased from 0.124 to 0.4656. Confirmation of the transformant using restriction and sequencing analysis showed that the gene encoding of SLPI domain C-terminal optimized in the pGEX 4T-2 plasmid was successfully transformed into E. coli TOP10 as novelty of this study. The optimized SLPIc gene in pGEX 4T-2 has a high probability of being expressed in E. coli based on in-silico analysis.
Accute Toxicity of Extract From Melinjo (Gnetum Gnemon L) Leaf With Fixed Dose Procedure Method Herlina Herlina; Annisa Amriani; Dina Permata Wijaya; Ayu Adelia Lestari
Indonesian Journal of Pharmaceutical Science and Technology Vol 9, No. 3, 2022
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v9i3.33683

Abstract

Melinjo leaves has several benefits as antidiabetic, antihyperglycemia, antioxidant and diuretic. In this experiment the acute toxicity test of ethanol extract of melinjo (Gnetum gnemon L). leaves on male white rats of wistar strain with fixed dose procedure method. The result of the screening of the phytochemical showed that the leaves of beluntas contained alcaloid, flavonoid, saponin, fenolic, and tanin. Ethanol extract of melinjo leaves was obtained by maceration using ethanol 70%. From the observational study, the dose 2000 mg/kg BW was chosen as starting dose for the main study. Experimental animal subject used in the main study were 10 male white rats of wistar which were divided into 2 group, normal group and treatment group with a dose of 2000 mg/kgBW. The result showed that there were no death or toxicity sign in all experimental animals. During the 14 days observation period, there were no significant changes in body weight of rats either in normal control group and treatmen group with a dose of 2000 mg/kg BW (p>0.05). Melinjo leaves had no effect on the macroscopic organs of liver, kidneys and heart of the test animals (p>0.05). The average level of biochemical parameters in the normal group is SGOT 54.13±11.428 U/L, SGPT 47.476±21.655 U/L, creatinine 0.632±0.199 mg/dL, and urea 14.46±2.267 mg/dL, while the 2000 mg/kg BW dose group is SGOT 60.192±14.198 U/L, SGPT 55.968±22.998 U/L, creatinine 0.756±0.204 mg/dL, and urea 16±2.561 mg/dL. It showed that the ethanol extract of melinjo leaves with a dose of 2000 mg/kgBW are the category of practically non-toxic.
Knowledge and Perception of Self-Medication of Cough Medication in Pedicab Drivers in Surabaya Amelia Lorensia; Rivan Virlando Suryadinata; Melly Eva Idamayanti; Gora Dirga Kusuma; Nyoman Yoga Diputra
Indonesian Journal of Pharmaceutical Science and Technology Vol 9, No. 3, 2022
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v9i3.33003

Abstract

Pedicab drivers who are exposed to pollution and have a smoking habit are at high risk of developing COPD (chronic obstructive pulmonary disease). The initial disturbance that occurs is a decrease in lung function which often causes complaints of cough symptoms. A person's knowledge and perception can influence how the pattern of self-medicating cough treatment so far. The purpose of this study was to determine the knowledge and perception of Self-medication of cough medicine. The research design was cross-sectional. The material of this research was in the form of information from subjects using direct questions and answers (interviews) with knowledge and perceptions of self-medicating cough medicine questionnaire. This research was conducted from September to December 2018 and used descriptive data analysis. The respondents involved were 163 peoples. Most respondents had a low level of knowledge of self-medication of cough medicine (97 of 163). The results of perception of self-medication of cough medicine indicated that most respondents had negative level (78 of 163). There was a relationship between knowledge and perception about self-medication of cough medicine (p=0.006). The proper health education strategy by increasing knowledge can also improve perceptions of self-medicine of cough medicine.
Isoniazid Microencapsulation With HPMCP HP-50 and HPMCP HP-55 (2:3) Coating Using Solvent Evaporation Method Hestiary Ratih; Gladdis Kamilah Pratiwi; Fikri Alatas; Mia Agustin; Bella Dewinta Saraswati
Indonesian Journal of Pharmaceutical Science and Technology Vol 9, No. 2, 2022
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v9i2.36513

Abstract

The combination formulation of TB drugs may cause interactions if these drugs are given simultaneously. Rifampin (RIF) decomposes in the stomach when given concurrently with isoniazid (INH), which results in a decrease in the bioavailability of RIF. The purpose of this study is to make INH microcapsules using HPMCP HP-50 and HP-55 coatings to prevent these interactions. The process of making INH: HPMCP HP-50 and HP-55 (2:3) microcapsules was done by using solvent evaporation method. The entrapment efficiency of INH: HPMCP HP-50 and HP-55 (2:3) were 83.21% and 91.57%, respectively. The dissolution test of INH: HPMCP HP-50 and HP-55 microcapsules met the requirements of the Indonesian Pharmacopoeia Edition V. The FTIR test showed that the microcapsules didn’t change the chemical composition of isoniazid or the coating on the microencapsulation so that it was concluded that no chemical reaction or decomposition occurred before and after the formation of the microcapsules. Scanning Electron Microscopy (SEM) showed a spherical microcapsule surface morphology and the active substance was well coated for INH: HPMCP HP-50 (2:3), while for INH: HPMCP HP-55 (2:3) the surface of the microcapsules was round but hollow. This study produces microcapsules that can provide a delayed release effect, so it is expected that INH: HPMCP HP-50 and HP-55 (2:3) microcapsules can be released in the intestines without interacting with RIF.
Molecular Docking Study: Phyllanthus niruri L.'s Active Compounds as Dengue Haemorrhagic Fever Therapy Riska Prasetiawati; Silviyanita Isna Seftila; Benny Permana; Novriyanti Lubis
Indonesian Journal of Pharmaceutical Science and Technology Suppl. 4, No. 1 (2022)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v1i1.42824

Abstract

Dengue Haemorrhagic Fever is a disease caused by the dengue virus through a mosquito vector Aedesaegypti. NS3 Helicase is known as one of nonstructural proteins consisting of some essential enzymesfor virus replication. Nowadays ivermectin has been developed as an anti-dengue haemorrhagic feverwith therapy target NS3 Helicase. The therapeutics drug for dengue haemorrhagic fever has not beenfound specifically. Methanol extract of meniran (Phyllanthus niruri L.) reported the activity to denguevirus with a concentration of 15,63 μg/mL. This research aimed to study the interactions and affinityof the active compound of meniran with the receptor (NS3 Helicase) and to know ADME and toxicityprofile. From 56 active compounds of meniran, there was one best candidate as dengue haemorrhagicfever therapy which has energy binding (ΔG) and Inhibition Constanta (IC) lower than native ligandand ivermectin, it is nirurin with energy binding -4.87 kcal/mol. These candidate compounds havegood absorption and distribution profiles so they are thought to be candidates for dengue fever therapyby targeting the NS3 Helicase receptor which is better than ivermectin and native ligands.
Antihyperuricemia Activity Of Kupa (Syzygium polycephalum) Seed Extracts In Male White Mice Ira Rahmiyani; Trian Nur'aripin; Anisa Pebiansyah; Resha R. Shaleha
Indonesian Journal of Pharmaceutical Science and Technology Suppl. 4, No. 1 (2022)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v1i1.42882

Abstract

Hyperuricemia is a clinical condition of increasing uric acid in the blood beyond normal. Increasedlevels of uric acid can accumulate monosodium urate crystals. Kupa fruit seeds contain flavonoids,saponins, tannins, and polyphenols which have antihyperuricemia activity. This study aims todetermine the best antihyperuricemia activity of several kupa seed extracts. The study was conductedin vivo using male white mice. Negative, positive, n-hexane extract, ethanol extract, and ethyl acetateextract were induced by acetylsalicylic acid 5.04 mg/20gBW of mice and fructose 67.2 mg/20gBWof mice orally for 3 days. On the 3rd day of treatment, the positive group was given allopurinol 0.997mg/20gBW and the 3 test groups were each given a different extract of 1.82 mg/20gBW mice orallyfor 7 days. The results showed the ethanol extract of Kupa fruit seeds had the best antihyperuricemiaactivity with a decrease in uric acid levels of 68.29%. Based on the results, the group of mice treatedwith ethanol extract had significantly different activity from the negative control group p <α (0.015<0.05). The ethanol extract showed the best antihyperuricemia activity approaching allopurinol as apositive control.
Antioxidant Activities of Scrub Body Lotion Extract of Surian Leaves (Toona sinensis) with Powder Scrub Date Seeds (Phoenix dactylifera) Uce Lestari; Yuliawati Yuliawati; Fathnur Sani; Yuliana Yuliana; Muhaimin Muhaimin
Indonesian Journal of Pharmaceutical Science and Technology Suppl. 4, No. 1 (2022)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v1i1.42828

Abstract

Free radicals from exposure to ultraviolet radiation (UVB) cause dull, rough, dry, cracked skin andpremature aging so that they can damage skin tissue and result in fatal skin cancer. To prevent this,antioxidants are needed. Plants that have potential as antioxidants are Surian leaves and date seedswhich contain lots of flavonoids and phenolics. To make it easier to use, it is processed into a scrubbody lotion using a date seed powder scrub (Phoenix dactylifera). This study aimed to find a scrubbody lotion formula that has the best physical properties and is stable during storage and the bestformula has the strongest antioxidant activity. Each formula contains ethanol extract of Surian leaves(Toona sinensis) with a concentration of 0.1%; 0.3%; 0.5%; 0.7%; 0.9%. After that, an evaluationof the physical properties of scrub body lotion preparation was carried out. From the results of theevaluation of the physical properties of the best scrub body lotion preparations, stable in storage andhedonic tests and safe for irritation tests, formula 5 contains ethanol extract of surian leaves with aconcentration of 0.9% and has an antioxidant activity of IC50 28,554 ppm with a very strong category.
Antibacterial Activities of Guava Leaves and Klutuk Banana by In Vitro as Antidiarrhea Ira Rahmiyani; Anna Yuliana; Mitha Anggitha; Vera Nurviana
Indonesian Journal of Pharmaceutical Science and Technology Suppl. 4, No. 1 (2022)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v1i1.42944

Abstract

Klutuk banana fruit and red shoots of guava leave added with salt used by Batra in Jayaratu SingaparnaVillage to treat diarrhea. The green shoots of guava used by the community in treating diarrhea thanklutuk bananas. This study aims to compare the activity of klutuk banana flesh (K) and red shoots ofguava leaves (M) with the addition of salt (G), which Batra commonly uses with green shoots of guavaleaves (H) used by the general public and their combinations to inhibiting Escherichia coli bacteria.This study was conducted in vitro with Simplicia test samples of guava leaf shoots and banana klutukcharacterized. Each sample was crushed, and the extract was taken and tested for antibacterial activity.Data analysis used One Way Analysis of Variant (Anova) and continued with the Least SignificantDifference (LSD) test. The results showed that samples M, H, K, HG, MHG, and MH providedresistance to E. coli ATCC 25922 with a significance value of 0.000 (p<0.05), whereas the HG and Hgroups did not show a significant difference (P >0.05). The red and green shoots of guava leaves, singlyor in combination have potent antibacterial activity. The variety of red shoots and green leaves of guavawith a mixture of salt (MHG) concentration of 100% (1:1:1) had the highest MIC value compared tothe other groups.
Molecular Docking and Toxicity from Temulawak Rhizome (Curcuma xanthorrhiza Roxb.) against COX-2 Meilinda setya praceka; Ellen N. Yunita; Cleopatra D. Semesta; Refitha N. Putri; Nazwa N. Mikdar; Elsa N. Sitinjak; Luthfi U. Setyawati; Muchtaridi Muchtaridi
Indonesian Journal of Pharmaceutical Science and Technology Suppl. 4, No. 1 (2022)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v1i1.43808

Abstract

Temulawak rhizome (Curcuma xanthorrhiza Roxb.) is a rhizome that comes from the Zingiberaceaetribe. Temulawak rhizome is commonly used as a traditional medicine in Indonesia as an antiinflammatory.The purpose of this study was to provide information on the potential of temulawakrhizome as a COX-2 inhibitor drug candidate and its toxicity to shrimp larvae (Artemia salina Leach.).The methods used are Lipinski Rule of Five prediction, PreADMET, molecular docking, pharmacophorescreening, and BSLT toxicity test. The results obtained show that the lowest Gibbs energy is producedby curcumin (-9.65 kcal/mol), has a pharmacophore hit value, meets the Lipinski rule of five, predictsa good pharmacophore profile, but curcumin has mutagenic properties and is classified as toxic afterbeing tested. with the BSLT method. So that it can be concluded that curcumin has the potential tobecome an anti-inflammatory drug, but further studies are needed and modifications to the molecularstructure of the compound can be carried out so that the tested compound can produce better activity.
Formulation And Characterization Of Buccal Film Nanoemulsion Apigenin As Antidiabetic Ardianes Firmansya; Fajar Setiawan; Lusi Nurdianti; Anna Yuliana
Indonesian Journal of Pharmaceutical Science and Technology Suppl. 4, No. 1 (2022)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v1i1.42829

Abstract

Apigenin (4`,5,7-trihydroxyflavone) is a flavonoid of subclass flavones that have antidiabetictherapeutic activity but limitations BCS Class II low solubility of 2.16 μg/L. To overcome theselimitations, the development of nanoemulsion formulation technology, increasing solubility increasesdissolution, absorption and bioavailability. It is incorporated into the buccal film for easy applicationand direct access to the systemic circulation. This study aims to obtain apigenin nanoemulsion withthe best characterization and buccal film that meets the characterization. The method was carried outexperimentally in the manufacture of nanoemulsions by spontaneous emulsification, a buccal filmby solvent casting, and the characterization. 10 nanoemulsion formulas met the characterizationwith globule size <20.34nm, polydispersity index <0.131, zeta potential close to 0mV, pH 6.23-6.59, %transmittance close to 100% and best F10 incorporated into buccal film has 29x the solubilitycompared to apigenin with p≤0.05. All buccal films met the characterization with F3 having a 2x fasteronset of release than F1&F2 with 86.07% diffusion and 97.9333 mg/sheet. Thus, it was concludedthat the formulation and characterization of buccal film fulfilled the characterization and F10 apigeninnanoemulsion increased the solubility 29-fold with the buccal film F3 having a faster onset of release.

Page 28 of 52 | Total Record : 511

 26   27   28   29   30 

Filter by Year

2014 2026


Reset
Filter By Issues
All Issue Vol 13, No 1 (2026) Vol 12 (2025): Vol. 12 Suppl. 2 (2025) 2025: Vol. 12 Suppl. 1 (2025) Vol 12, No 3 (2025) Vol 12, No 2 (2025) Vol 12, No 1 (2025) 2024: Suppl. 6, no. 3 (The 3rd Mandala Waluya International Conference on Pharmaceutical Science and Vol 11, No 3 (2024) Vol 11, No 2 (2024) Vol 11, No 1 (2024) 2024: Suppl. 6, No. 2 (Universitas Halu Uleo Conference) 2024: Suppl. 6, No. 1 (Special Issue for Mulawarman Pharmaceutical Conference) Suppl. 5, No. 2 (2023) Special Issue for The 3rd Bandung International Teleconference on Pharmacy (B Vol 10, No 3 (2023) Vol 10, No 2 (2023) Vol 10, No 1 (2023) Suppl. 5, No. 1 (2023) Vol 9, No. 3, 2022 Vol 9, No. 2, 2022 Vol 9, No 1 (2022) Suppl. 4, No. 1 (2022) Vol 8, No 3 (2021) Vol 8, No 2 (2021) Vol 8, No 1 (2021) Suppl. 3, No. 1 (2021) Vol 7, No 3 (2020) Vol 7, No 2 (2020) Vol 7, No 1 (2020) Vol 6, No 3 (2019) Vol 6, No 2 (2019) Vol 6, No 1 (2019 In Press) Vol 6, No 1 (2019) Suppl. 2, No. 3 (2019) Suppl. 2, No. 2 (2019) Suppl. 2, No. 1 (2019) Vol 5, No 3 (2018) Vol 5, No 3 (2018) Vol 5, No 2 (2018) Vol 5, No 2 (2018) Vol 5, No 1 (2018) Vol 5, No 1 (2018) Suppl 1, No. 1 (2018) Suppl 1, No. 1 (2018) Vol 4, No 3 (2017) Vol 4, No 3 (2017) Vol 4, No 2 (2017) Vol 4, No 2 (2017) Vol 4, No 1 (2017) Vol 4, No 1 (2017) Supp 1, No 1 (2017) Supp 1, No 1 (2017) Vol 3, No 3 (2016) Vol 3, No 3 (2016) Vol 3, No 2 (2016) Vol 3, No 2 (2016) Vol 3, No 1 (2016) Vol 3, No 1 (2016) Vol 2, No 3 (2015) Vol 2, No 3 (2015) Vol 2, No 2 (2015) Vol 2, No 2 (2015) Vol 2, No 1 (2015) Vol 2, No 1 (2015) Vol 1, No 2 (2014) Vol 1, No 2 (2014) Vol 1, No 1 (2014) Vol 1, No 1 (2014) More Issue