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SYNTHESIS OF METAL-ORGANIC (COMPLEXES) COMPOUNDS COPPER(II)-IMIDAZOLE FOR ANTIVIRAL HIV CANDIDATE Sucipto, Teguh Hari; Martak, Fahimah
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 7 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v5i7.1204

The human immunodeficiency virus (HIV) is viruses known as rotaviruses. Potential target for therapeutic is reverse transcriptase (RT), possesses an RNA-dependent DNA polymerase, DNA-dependent DNA polymerase and ribonuclease H fuctions. Imidazoles have high anti-HIV inhibitory activity, some derivates of imidazole reported drugs. 8-chloro-2,3-dihydroimidazole[1,2-b][1,4,2]benzodithiazine-5,5-dioxides and 9-chloro-2,3,4-trihydropyri-mido[1,2-b][1,4,2]benzodithi-azine-6,6-dioxides. This compounds succesfully identified anti-HIV activity. Copper is a bio-essential element and copper complexes have been extensively utilized in metal mediated DNA cleavage for the generation of activated oxygen species. It has been reported that teraaza macrocyclic copper coordination compounds have anti-HIV activities. Studies have shown that these macrocyclic complexes can react with DNA in different binding fashions and exhibit effective nuclease activities. Complex compounds are compounds in which there is an atom that acts as the central atom and trotter group of molecules that can be either neutral or charged ions. Application a metal-organic (complex) compounds, especially copper metal and derivates of imidazole. So, in this study can explore new anti-HIV candidate.

CELLULAR IMMUNITY ACTIVATION METHOD BY STIMULATING RD1 COMPLEX PROTEINS AS VIRULENCE MARKER ON Mycobacterium tuberculum TO ESTABLISH DIAGNOSIS ON TUBERCULOSIS AND LATENT TUBERCULOSIS INFECTION Setiabudi, Rebekah; Mertaniasih, Ni Made; Didik Handijatmo, Didik; Setyoningrum, Retno Asih
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 7 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v5i7.1205

This study was intended to invent a simpler and more affordable method to establish diagnosis on Tuberculosis (TB) and Latent Tuberculosis infection (LTBI). Similar to âQuantiferon TB Gold In Tubeâ (QFT-GIT) and T.SPOT.TB methods, the researchers also utilized âearly secreted antigenic target 6kDaâ (ESAT-6) and âcultur filtrate protein 10kDaâ (CFP-10) proteins to be induced on the specimen. ESAT-6 and CFP-10 are commercial products used to induce interferon gamma (INF-Î³) which were to be read using sophisticated and expensive equipment. This study was intended to conduct an analysis on effective cocktail protein modification, i.e. ESAT-6, CFP-10 and Ag85A/B/C, with high validity to detect cellular immunity activity through in vitro examination on peripheral blood monocyte cells of Tuberculosis-suspected patients or patients with latent tuberculosis infection. Peripheral Blood Monocyte Cells (PBMCs) activity on children tuberculosis patient or Latent Tuberculosis Infection (LTBI), adult tuberculosis patient or LTBI, which induced by cocktail protein modification and not induced, were analyzed microscopically. The activity of PBMCs on children and adult tuberculosis patient or LTBI induced by RD1 secretory proteins: ESAT-6, CFP-10, Ag85A/B/C was higher compared to PBMCs which had not been induced by the secretory proteins. Cellular debris and monocyte cells with abnormal shapes were found on PBMCs which had been induced by RD1 secretory proteins at 8 th day after culture.

Mycobacterium leprae BACILLEMIA IN BOTH TWINS, BUT ONLY MANIFEST AS LEPROSY IN ONE SIBLING Sukmawati, Netty; Agusni, Indropo; Listiawan, M. Yulianto; Prakoeswa, Cita Rosita S.; Adriaty, Dinar; Wahyuni, Ratna; Iswahyudi, Iswahyudi
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 7 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v5i7.1206

Leprosy in twins is rarely reported. A 19 years-old male student, from Lamongan district, was diagnosed as Multibacillary (MB) leprosy in the Skin and STD Clinic of Dr. Soetomo General Hospital Surabaya. Multiple anesthetic skin lesions were found, but the bacteriologic examination was negative for Acid Fast Bacilli (AFB). Histopathology examination support the diagnosis of BL type of leprosy. His twin brother that has been lived together since born until present seems healthy without any complaints of skin lesions and have no signs of leprosy. When a serologic examination for leprosy was performed, a high anti PGL-1 antibody level was found in patient (IgM anti PGL-1 2937 and IgG anti PGL-1 3080 unit/ml) while his healthy twin brother showed only low level (IgM 745 and IgG 0 unit/ml). Interestingly when a PCR study was performed to detect M.leprae in the blood, both of them showed positive results. Using the TTC method, a genomic study of for M.leprae, it is revealed that both samples were identic ( 27x TTC repeats). According to patientâs history, he had a traffic accident and got a wound in the knee seven years ago, while the skin lesions seems started from this area around three years ago before it spread to other parts of the body. The patient was treated with Multi-drug therapy (MDT) while his sibling got a prophylactic treatment for leprosy. After 6 months of treatment, the leprosy skin lesions were diminished and the serologic anti PGL-1 has been decreased. His healthy brother also showed a decrease in anti PGL-1 level and no skin signs of leprosy.

CYTOTOXICITY OF JUSTICIA GENDARUSSA BURM F. LEAF EXTRACTS ON MOLT-4 CELL Widiyanti, Prihartini; Prajogo, Bambang; Hikmawati, Ni Putu Ermi
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 7 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v5i7.1207

Justicia gendarussa Burm f. (Acanthaceae) is known for its activity as a male contraceptive and anti-HIV properties. The present study was designed to evaluate extracts of J. gendarussa for cytotoxicity activity against MOLT-4 cells. The cytotoxic activity of the fractionated-extract and 70% ethanol extracts of J. gendarussa leaves on MOLT-4 cells were evaluated using a WST-1 assay. The treatment cells, control cells without treatment and control media were also tested in duplicate. The absorbance was measured at a wavelength of 450 nm using a microplate absorbance reader (Bio-Rad). The average absorbance measures formazan produced by viable cells that metabolize the WST-1 reagent. Then the data was analyzed with regression analysis Microsoft Excel 2007 program to determine the concentration with 50% cell viability (50% Cytotoxicity Concentration, CC50). The CC50 values of the fractionated-extract and 70% ethanol extract of J. gendarussa leaves were 94 Î¼g/ml and 78 Î¼g/ml, respectively. The cytotoxicity of fractionated-extract and 70% ethanol extract of J. gendarussa leaves were not significantly different (p > 0.05). It can be concluded that the fractionated-extract and 70% ethanol extract of J. gendarussa leaves are not toxic to MOLT-4 cells.

BRADYCARDIA AND TACHYCARDIA DETECTION SYSTEM WITH ARTIFICIAL NEURAL NETWORK METHOD S, Delima Ayu; Arisgraha, Franky; Apsari, Retna
Indonesian Journal of Tropical and Infectious Disease Vol. 3 No. 2 (2012)
Publisher : Institute of Topical Disease Universitas Airlangga

Heart disease is one disease with high mortality rate in the world. Based on WHO records from 112 countries at 2004, the rate is 29% of all deaths each year. Medical devices are necessary to diagnose one's health as an indication of a disease. Nowadays, Indonesia still imports medical devices, for the diagnosis of heart failure, from abroad. This research aims to assist the monitoring of cardiac patients with bradycardia and tachycardia appearances of message condition patient's heart rate at the same time. The results were displayed with the output of bradycardia condition of the heart rate (heart rate less than 60 beats per minute) or tachycardia (heart rate over 100 beats per minute). The system displayed the data read from the heart to the PC embedded system to monitor the condition of the patients under decisions based on backpropagation neural network. Classification system could be performed quite well, training data and by testing the 10 pieces, the optimal weight gain was 1727 iteration, the learning rate was 0.1122, and the error was below 0.001 (0.0009997).

ROLE OF BREAK CLUSTER REGION (BCR) - ABELSON MURINE LEUKIMIA (ABL) EXAMINATION IN CHRONIC MYELOGENOUS LEUKEMIA (CML) Sosiawan, Agung
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 2 (2014)
Publisher : Institute of Topical Disease Universitas Airlangga

Chronic myelogenous leukemia (CML) is a clonal bone marrow stem cell disorder associated with a characteristic chromosomal translocation called the Philadelphia chromosome which caused a proliferation of mature granulocytes (neutrophils, eosinophils and basophils) and their precursors, increasing unregulated growth of predominantly myeloid cells in the bone marrow and its accumulation in the blood. As myeloproliferative disease, Chronic Myelogenous Leukemia or CML is a malignancy of the sixth-highest, reaching 15% of all blood malignancies in adults with an incidence of 1.1 per 100,000 population (Ugroseno, 2012). The CML diagnosis is made based on a presence of Philadelphia chromosome due to the existence of a reciprocal translocation of chromosomes 9 and chromosome 22 t (9.22), and raises the fusion of Break Cluster Region (BCR) gene of chromosome 22 on band q11 by Abelson Murine Leukemia (ABL). The fused BCR-ABL gene has BCR sequences of different length, so it produces a protein that has a different molecular weight. Despite having different length of BCR sequences, however, the length of fuses ABL gene sequence is constant. Associated with this different BCR sequence length are the three variations of the BCR-ABL gene fusion. The first variation is a Major Break Cluster (M-BCR), the BCR gene break is found in exon 2 in e13-E14 region. This type of CML is the fusion of BCR exon b2 or b3 to ABL exon a2, forming two major transcripts of the b2a2 or b3a2, which has a protein product with 210 kD weight or referred to as p210. The second variation is Minor BCR (m-BCR), which has e1a2 fusion. CML with BCR-ABL gene fusion of this type has a protein product with a molecular weight of 190 kDa or called p190. The third variation is micro-BCR (m-BCR), with BCR gene break between exons E19 and e20b that form mRNA transcripts e19a2, with BCR-ABL protein P230. This fused gene can be detected with qualitative multiplex PCR.

UPDATE MANAGEMENT CONCURRENT INFECTION BETWEEN DENGUE VIRAL AND SALMONELLA Wikanesthi, Dyah; Sari, Desiana W; Chilvia, Eva; Soedirham, Oedojo; Kurniasari, Lely; Soegijanto, Soegeng
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 3 (2014)
Publisher : Institute of Topical Disease Universitas Airlangga

Since Januari 2013, Soerya Hospital has found many cases with positive result of IgM Salmonella along with NS1 or IgM & IgG Dengue. The clinical manifestations mostly are high fever, headache, vomiting, malaise and plasma leakage. Some of them with convulsion and unconsciousness. Therefore in order to get well of care management, this clinical phenomena should be studied carefully. The aim of this research is to get update management concurent Dengue Viral and Salmonella infection. Observational study had been done, since Januari 2013 until Juli 2013. Purposive sampling in 30 case of concurent Dengue Viral and Salmonella infection compared with 30 case of Dengue Viral infection alone. Diagnosis has published based on WHO 2011 criteria. By using anti vomiting drug, anti pyretic, anti convulsion and antibiotic for Salmonella infection and rehidration using Ringer Acetate, combining Ringer Asetat andDextrose 5% or combining Ringer Asetat Saline 0,225% or solution of Dextrose 5% and Saline 0,45 during 4–5 days hospitalization. The result show that all cases were recovered and got well. There is no significant different between concurent Dengue Viral and Salmonella infection compared with Dengue Viral infection alone. Some cases showed that length time to stay in hospital become1–2 days longer. It was due to delayed getting antibiotic for Salmonella infection. All cases had got first drugs accurately in a clinical manifestation that has been daily showed. It was as a problem solving for saving all the cases.

CYTOTOXICITY OF JUSTICIA GENDARUSSA BURM F. LEAF EXTRACTS ON MOLT-4 CELL Widiyanti, Prihartini; Prajogo, Bambang; Hikmawati, Ni Putu Ermi
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 1 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

Justicia gendarussa Burm f. (Acanthaceae) is known for its activity as a male contraceptive and anti-HIV properties. The present study was designed to evaluate extracts of J. gendarussa for cytotoxicity activity against MOLT-4 cells. The cytotoxic activity of the fractionated-extract and 70% ethanol extracts of J. gendarussa leaves on MOLT-4 cells were evaluated using a WST-1 assay. The treatment cells, control cells without treatment and control media were also tested in duplicate. The absorbance was measured at a wavelength of 450 nm using a microplate absorbance reader (Bio-Rad). The average absorbance measures formazan produced by viable cells that metabolize the WST-1 reagent. Then the data was analyzed with regression analysis Microsoft Excel 2007 program to determine the concentration with 50% cell viability (50% Cytotoxicity Concentration, CC50). The CC50 values of the fractionated-extract and 70% ethanol extract of J. gendarussa leaves were 94 Î¼g/ml and 78 Î¼g/ml, respectively. The cytotoxicity of fractionated-extract and 70% ethanol extract of J. gendarussa leaves were not significantly different (p > 0.05). It can be concluded that the fractionated-extract and 70% ethanol extract of J. gendarussa leaves are not toxic to MOLT-4 cells.

Natural Growth Factor: Platelet Rich Plasma Stimulates Proliferation of Fibroblast Cell Culture Setiawati, Ernie Maduratna
Indonesian Journal of Tropical and Infectious Disease Vol. 1 No. 2 (2010)
Publisher : Institute of Topical Disease Universitas Airlangga

Platelet rich plasma(PRP) is a source of many natural growth factor that can modulate wound healing. PRP has become a valuable adjunct in dentistry. Fibroblast cell in ligament periodontal play on important role in periodontal regeneration. The study was performed to investigate the influence PRP 10%–100% on the proliferation of fibroblast cell culture in vitro. A fibroblast culture was estabilished from baby hamster kidney (BHK-21) were tested on proliferation was measured with MTT assay after induced PRP 10–100%. We showed that PRP stimulate the proliferation of fibroblast, PRP 80% as the optimal choice to a good enough proliferative stimulus. PRP has proven to be effective at improving surgical results in periodontal surgery. PRP also show promise in periodontal regenerative medicine.

NORWEGIAN SCABIES IN AIDS PATIENT: A CASE REPORT Pratamasari, Meita Ardini; Agusni, Indropo; Prakoeswa, Cita Rosita Sigit; Astari, Linda; Sandhika, Willy
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 2 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

Scabies is a skin infection caused by Sarcoptes scabiei var. hominis. This disease may present severe clinical manifestations in immune-compromised patient, well-known as Norwegian scabies or crusted scabies.A 36-year old man with AIDS had chief complaint thick crust almost all over his body in this case. History of household member infected by scabies before was present. Clinical findings show hyperpigmented macules unsharply marginated, covered with thick scales and accompanied by papules, fissures, and erotion. T cell CD4 level was 12 cell/Î¼L. Scraping examination showed scabies infection and so did the histopathology examination. This patient was treated by topical Permethrin 5% combined with 2-4 ointment application in between permethrin usage. Before topical scabicide was given, thick crust was previously treated by topical urea 10% and wet dressing by normal saline. On day 14 after the patient first came there was lesion improvement.

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Kota surabaya,

Jawa timur

INDONESIA