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Indonesian Journal of Tropical and Infectious Disease

IJTID
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Published by Universitas Airlangga

ISSN : 20851103 EISSN : 23560991 DOI : -

Core Subject : Health, Science,

Earth & Planetary Sciences Health Professions Medicine & Pharmacology Public Health

This journal is a peer-reviewed journal established to promote the recognition of emerging and reemerging diseases specifically in Indonesia, South East Asia, other tropical countries and around the world, and to improve the understanding of factors involved in disease emergence, prevention, and elimination. The journal is intended for scientists, clinicians, and professionals in infectious diseases and related sciences. We welcome contributions from infectious disease specialists in academia, industry, clinical practice, public health, and pharmacy, as well as from specialists in economics, social sciences and other disciplines.

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Articles 382 Documents

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EVALUATION OF Salmonella sp CONTAMINATION AND ITS ANTIBIOTICS RESISTANCE PATTERNS ISOLATED FROM BROILER MEAT SOLD AT WET MARKET IN CENTER OF SURABAYA Aprillian, Risky; Rahardjo, Dadik; Koesdarto, Setiawan
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 6 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

Antibiotic resistance now days become a main issue to the medical researches as found many positive result of antibiotic resistance test. One of the causes of antibiotic resistance is using antibiotic as a feed additive to animal. Bacteria that are resistant to antibiotics can be a danger to humans, in this case the resistant bacteria as a result of treatment errors animals, especially chickens that uses low-dose antibiotics as growth promoters. This study aimed to determine the contamination of Salmonella sp and its antibiotics resistance patterns of Salmonella sp isolated from broiler meat sold at wet market in the Center of Surabaya: (a) Pasar Kembang, (b) Pasar Kupang, (c) Pasar Dukuh Kupang, (d) Pasar Kedungsari, (e) Pasar Kedungdoro and (f) Pasar Keputran. The method that used in this study was bacteriological isolation and identification method. The method started with pre-enrichment using Buffered Pepton Water, selective enrichment using Tetrathionate Broth and Selenite Cysteine broth, selective media using Salmonella-Shigella Agar, Biochemical test using Triple Sugar Iron Agar, Simon Citrate, Methyl Red – Voges Proskauer, and Sulfide Indol Motility, and followed with susceptibility test according to Kirby-Bauer method using Mueller-Hinton Agar. The antibiotics that used in susceptibility test were: (a) Meropenem, (b) Ampicillin Sulbactam, (c) Amikacin, (d) Ofloxacin and (e) Nalidixic Acid. The results of this study were found 90% or 27 of 30 samples positive contaminated with Salmonella sp. The results of antibiotics resistance from 27 isolates 0% were resistant to Meropenem, 0% were resistant to Amikacin; 3.7% were resistant to Ampicillin-Sulbactam; 11.1% were resistant to Ofloxacin and 44.4% were resistant to Nalidixic Acid.

Serotype and Clinical Performance of Dengue Virus Infection on the Year 2009 Soegijanto, Soegeng; Darmowandowo, Widodo; Ginting, Amor Peraten; Yamanaka, Atsushi
Indonesian Journal of Tropical and Infectious Disease Vol. 1 No. 2 (2010)
Publisher : Institute of Topical Disease Universitas Airlangga

Dengue hemorrhagic fever is one of the important health problem in Indonesia, mortality rate is becoming decrease but many dengue shock syndrome cases is very difficult to be help. Previous study showed that some of DEN 2 and DEN 3 virus cases could show a clinical performance of severe dengue virus infection such as dengue shock syndrome. There are four serotype of dengue virus infection can cause primary and secondary infection. The aim of this research is to know the relationship between clinical performance of dengue virus infection and serotype dengue virus and also to know the role of primary and secondary infection and age of dengue virus cases. A prospective analytic observational study, which was conducted in Dr. Soetomo hospital since January 2009. RT-PCR was used to attempt to identify the infecting serotype from dengue virus isolated using vero cell. Antibody responses were measured by ELISA and clinical manifestation were measured with the WHO criteria 1997. Dengue serotype identification by RT-PCR was 70 patients. Virus types were DEN-2 65(92.8%), DEN-1 3(4.2%), and DEN-3 2(2.8%). Patients with DEN-1 genotype IV were more trend severe disease DSS and unusual infection. Commanly usually secondary exposure cause more severe clinical manifestation than primary exposure (p = 0.035) but in this study found that all of DEN-1 genotype IV, primary or secondary infection to show severe clinical manifestation of dengue virus infection. We can conclude that DEN-2 was the most dominant serotype in Dr. Soetomo Hospital. On Primary and secondary infection, DEN-1 genotype IV showing more severe than DEN-2 and DEN-3.

CHARACTERIzATION of CD4 + T LYMPHOCYTE FROM BONE MAROW STEM CELL USING INDIRECT IMMUNOFLUORESENCE FOR HIV & AIDS TREATMENT Purwati, Purwati; Nasronudin, Nasronudin; Rantam, Fedik Abdul
Indonesian Journal of Tropical and Infectious Disease Vol. 1 No. 3 (2010)
Publisher : Institute of Topical Disease Universitas Airlangga

Acquired immune deficiency syndrome (AIDS) is caused by Human Immunodeficiency Virus (HIV). At the beginning of infection, gp120 virus interacts with CD4 receptor at the surface of the target cell. The interaction between gp120 and CD4 leads to the occurrence of the binding of specific chemokine receptor CXCR4 and CCR5, which are also present on the membrane of the target cell. Therefore, CCR5 and CXCR4 also determine the fate of the target cell. It is the performance of CCR5 and CXCR4, guided by controlling gene that determines susceptibility or resistance to HIV infection. Coding gene CCR5 may mutate to become protective or resistant against HIV infection. In homozygote individuals, it tends to be resistant against infection, while in heterozygote individuals it tends to be susceptible to HIV infection. Objective: To characterize TCD4 lymphocyte in the next that is resistant against HIV infection by using gene therapy deletion 32 CCR5 to use for HIV & AIDS treatment. Method: Sample collection, mononucleated cell collection, lymphocyte culture, CD4 identification, CCR5 variance analysis, co-cultivation with PBMC HIV and comparison to control. Result: This study was performed in several steps, such as mononucleated cell isolation, followed with cell culture, lymphocyte purification, lymphocyte and CD4 expression identification. Conclusion: Lymphocyte T CD4 had been mature after seven passages, once passage is about 5 days so for maturity lymphocyte T CD4 need 35 days and that cell as be candidate to resistant against HIV infection by using gene therapy deletion 32 CCR5 to use for HIV & AIDS treatment.

RECCURENT LARYNGEAL PAPILLOMA Purnami, Nyilo; Fathoni, Rizka
Indonesian Journal of Tropical and Infectious Disease Vol. 3 No. 1 (2012)
Publisher : Institute of Topical Disease Universitas Airlangga

A case of respiratory papillomatosis was reported. The patient suffered from the disease since eight months old with chief complaint progressive hoarseness and dyspnea. It was diagnosed with respiratory papillomatosis and scheduled for performing tracheotomy and continued with the first microlaryngeal surgery (MLS). Decanulation was taken after 2nd surgery of removing papillomas. Finally was reported she got serial of surgery for 22 times during 18 years of age. It was costly and deteriorating quality of life. The problem remains persisted because of frequent recurrences and need for repetitive surgeries. Specimen biopsy for histologic examination was shown the signs of HPV infection, papilomatic coated squamous epithel with mild dysplasia and coilocytosis. The threatening of upper airway obstruction is the main important reason for patient's coming. The patency of airway assessed by Direct Laryngoscopy then the next treatment was decided with schedule of Micro Laryngeal Surgery (MLS). Finally the MLS treatment is just only for temporarilyrecovery. A further research to define the proper treatment in the future is required, especially for prevention of the diseases related to the viral causes of infection.

Molecular Surveillance of Dengue Virus Serotype Using Polymerase Chain Reaction in Surabaya 2013 Sucipto, Teguh Hari; Labiqah, Amaliah; Churrotin, Siti; Ahwanah, Nur; Mulyatno, Kris Cahyo; Soegijanto, Soegeng; Kotaki, Tomohiro; Kameoka, Masanori; Konishi, Eiji
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 1 (2014)
Publisher : Institute of Topical Disease Universitas Airlangga

Dengue is one of the infectious diseases which is endemic in the tropical and sub-tropical country. The disease found in Indonesia Surabaya, 1968. The symptoms of Dengue virus infections are two kinds, first DF (Dengue Fever), second DHF (Dengue Hemorrhagic Fever). This infectious disease transmitted by Aedes aegypti mosquito. Mosquitoes breed in clean water areas. More than 100,000 cases of DF/DHF ccurred in Indonesia every year. The purpose of this study were to provide information and the spread of dengue virus types in Surabaya from January 2013 to September 2013. The nalysis technique used to determine the type of dengue virus nfectionwas used PCR (Polymerase Chain Reaction). The results obtained 69% DENV-1, 27% DENV-2 isolates, 4% isolates DENV-3, and 0% DENV-4 isolates.

PREDICTION OF DENGUE FEVER EPIDEMIC SPREADING USING DYNAMICS TRANSMISSION VECTOR MODEL Widyaningrum, Retno; Partiwi, Srigunani; Rahman, Arief; Sudiarno, Adithya
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 2 (2014)
Publisher : Institute of Topical Disease Universitas Airlangga

Increasing number of dengue cases in Surabaya shows that its city has high potential of dengue fever epidemic. Although some policies were designed by Surabaya Health Department, such as fogging and mosquito's nest eradication, but these efforts still out of target because of inaccurate predictions. Ineffectiveness eradication of dengue fever epidemic is caused by lack of information and knowledge on environmental conditions in Surabaya. Developing spread and prediction system to minimize dengue fever epidemic is necessary to be conducted immediately. Spread and prediction system can improve eradication and prevention accuracy. The transmission dynamics vector simulation will be used as an approach to draw a complex system of mosquito life cycle in which involve a lot of factors. Dynamics transmission model used to build model in mosquito model (oviposition rate and pre adult mosquito), infected and death cases in dengue fever. The model of mosquito and infected population can represent system. The output of this research is website of spread and prediction system of dengue fever epidemics to predict growth rate of Aedes Aegypti mosquito, infected, and death population because of dengue fever epidemics. The deviation of infected population is 0,519. The model of death cases in dengue fever is less precision with the deviation 1,229. Death cases model need improvement by adding some variables that influence to dengue fever death cases. Spread of dengue fever prediction will help the government, health department to decide the best policies in minimizing the spread of dengue fever epidemics.

RELATIONSHIP BETWEEN CLINICAL MANIFESTATIONS AND ANTIBODY SERUM IN OUTBREAKS ANTHRAX Redhono, Dhani
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 5 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

Introduction: Anthrax is a zoonotic disease that often affects the grass-eating animals, which occurs due to the entry of spores into the bodies of animals and can be transmitted to humans. This disease often appear in certain seasons and occurs in endemic areas, including Indonesia. Cutaneous anthrax is the clinical manifestations that often arise on outstanding events in the area. This study aims to determine how the relationship between the clinical manifestations of the serum antibodies in people who are exposed to anthrax. Material and methods: This study is an observational cross sectional analytic approach, in people exposed to anthrax to assess the clinical manifestations and antibody serum Anthrax. Results: Obtained in this study respondents were 101 people with a history of contact with animals suffering from anthrax. The number of respondents with the highest age distribution was 31 to 40 years by 42%, and most were female gender, which is 57.7%, the highest level of education is 74% finished elementary school. Forty-four percent of working as a housewife. Risk factors are the most direct contact with and consume the flesh of animals as much as 34.6%. Results of Ig G antibody serum showed 50% negative, 15.4 borderline and 34.6% positive. Clinical manifestations that occur in the skin as much as 13.5%, that is the eschar on all respondents and 92.8% showed positive Ig G. While 86.5% did not show any clinical signs of anthrax, of that number 25.5% with Ig G positive, 16.6% and 57.7% showed borderline negative with p 0.02. Conclusion: There was a significant association between the clinical manifestation with antibody serum anthrax. But also found a positive Ig G without the appearance of clinical signs in the skin.

OPTIMATION OF 48 kHz ULTRASONIC WAVE DOSE FOR THE INACTIVATION OF Salmonella typhi Lestari, Dwi May; Prijo, Tri Anggono; Astuti, Suryani Dyah
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 4 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

This study was aimed to determine the effect of ultrasonic dose exposure which could decrease the viability of Salmonella typhi by using the variation of exposure time (15, 20, 25, and 30 minutes) and volume of bacterial suspension (2, 4, 6, and 8 ml) at constant power. The sample used was Salmonella typhi. Ultrasonic wave transmitter was a piezoelectric tweeter with 0,191 watts of power and 48 kHz frequency generated by the signal generator. Piezoelectric tweeter was a kind of transducer which converted electrical energy into ultrasonic energy. This research was an experimental laboratory with a completely randomized design. The decrease of bacterial percentage was calculated by using TPC (Total Plate Count). Data were analyzed by using One Way Anova. The results showed that the variation of exposure time and volume of bacterial suspension gave significant effect on the percentage of Salmonella typhi kill. The most optimal of ultrasonic dose exposure to kill Salmonella typhi was 281.87 J/ml with 100% bacterial kill.

HEPATITIS C VIRUS INFECTION IN HEMODIALYSIS PATIENTS: COMPARISON OF THE SURABAYA DIALYSIS CENTER AND JUNTENDO UNIVERSITY HOSPITAL DIALYSIS CENTRE santoso, djoko; pranawa, pranawa; Yogiantoro, Moh.; Widodo, Widodo; Wardana, Aditia; Mardiana, Nunuk; Mohani, Chandra Irwanadi; soewanto, soewanto; Shou, Ichiyu; Maeda, Kunimi; Hamada, chieko; Fukui, Mitsumine; Horikoshi, Satoshi; Tomino, Yasuhiko
Indonesian Journal of Tropical and Infectious Disease Vol. 1 No. 3 (2010)
Publisher : Institute of Topical Disease Universitas Airlangga

Hepatitis C virus infection is highly prevalence in chronic hemodialysis (HD) patients. The present study will compare prevalence of HCV positive population in difference countries where there are great contrasts in and diversity of care available to patients who have end stage renal disease. All serum samples of the 100 patients were tested for HCV antibodies, using third-generation enzyme immunoassay. The prevalence of anti-HCV was correlated with a history of blood transfusion and with duration of hemodialysis. HCV prevalences were 88% of Surabaya group and 6% of Juntendo Group, respectively. In Surabaya Group, prevalence of HCV positive was high and the risk factors are not only those of the Juntendo Group, but also a combination of poor living conditions, frequent blood transfusions, and lack of adherence. Much needs to be studied about the role of universal screening and effective techniques for primary prevention in Surabaya Group

BASIC MECHANISM OF HYPERBARIC OXYGEN IN INFECTIOUS DISEASE Widiyanti, Prihartini
Indonesian Journal of Tropical and Infectious Disease Vol. 2 No. 1 (2011)
Publisher : Institute of Topical Disease Universitas Airlangga

Hyperbaric oxygen therapy (HBOT) is the inhalation of 100 percent oxygen inside a hyperbaric chamber that is pressurized to greater than 1 atmosphere (atm). HBOT causes both mechanical and physiologic effects by inducing a state of increased pressure and hyperoxia. HBOT is typically administered at 1 to 3 atm. While the duration of an HBOT session is typically 90 to 120 minutes, the duration, frequency, and cumulative number of sessions have not been standardized. HBO has been use widely in treating gangrene diabetic, stroke, osteomyelitis and accelerating wound healing. The use of HBO in infectious disease is wide, so the mechanism of hyperbaric oxygen in infectious disease should be well-understand. This understanding could bring the proper and wise management of infectious disease and to prevent the side effect of each therapy.

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