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Silkworm cocoon (Bombyx mori) accelerates wound healing in skin excision: a study on macrophage and VEGF
Sudirman, Sudirman;
Hasan, Cahya Yustisia;
Arindra, Pingky Krisna
Majalah Kedokteran Gigi Indonesia Vol 10, No 3 (2024): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada
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DOI: 10.22146/majkedgiind.87352
Silkworm cocoon (Bombyx mori) is a natural polymer composite and largely used as bio-functional material for wound healing. It consists of fibroin and sericin protein that has antibacterial effect. This study aimed to investigate the effect of silkworm cocoons (Bombyx mori) wound dressing on the number of macrophages and VEGF expression in skin excision. The subject of this study was 12 Wistar rats, which were grouped based on the duration of wound dressing application (3rd day and 6th day) and the use of dressing materials (n = 6). The rats were anesthetized with ketamin and xylazine prior to wound excision. A punch biopsy wound excision with 4 mm diameter of subcutaneous depth was made on both sides of the rat’s back, with the right side dressed with silkworm cocoon as the treatment group and the left side dressed with moist gauze application as the control group. Hematoxylin-eosin (HE) staining was performed to observe the number of macrophage cells. Immunohistochemical staining using an anti-VEGF antibody was performed to observe the expression of VEGF. Data were analyzed using a two-way ANOVA and an Independent t-test with confidence interval of 95%. Statistical analysis demonstrated a significantly higher number of macrophages in the silkworm cocoon wound dressing group on 6 days post-application (p = 0.026) and significantly higher VEGF expression in the silkworm cocoon wound dressing group on 3 days post-application (p = 0.002) and on 6 days post-application (p = 0.044). Silkworm cocoon (Bombyx mori) wound dressing can increase the number of macrophages and VEGF expression in wound excision model in Wistar rat.
Is breastfeeding duration associated with caries in stunted toddlers? a cross-sectional study
Hidayah, Riski Amalia;
Rochmawati, Mutia;
Kinasih, Amelia Sekar
Majalah Kedokteran Gigi Indonesia Vol 10, No 3 (2024): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada
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DOI: 10.22146/majkedgiind.88996
Stunting remains a significant nutritional concern in Indonesia, potentially affecting physical and cognitive development. Stunting may lead to oral health issues, specifically dental caries, due to salivary gland atrophy in malnourished children. While dental caries have multiple contributing factors, breast milk contains protective proteins and antibacterial properties that may influence caries development. This study aims to investigate the association between breastfeeding duration and dental caries occurrence in stunted and non-stunted toddlers. An analytical observational study with a cross-sectional approach was conducted using quota sampling of 30 toddlers attending Sumingkir Village Posyandu. Spearman correlation analysis showed no significant correlation between breastfeeding duration and caries in both stunted (p = 0.68) and non-stunted groups (p =0.66). Independent T-test revealed no significant mean differences between the groups (p < 0.05). However, the Mann-Whitney test demonstrated a significant median difference in caries (p = 0.006) between stunting (4(0-6)) and non-stunting groups (0(0-18)). This study concludes that breastfeeding duration showed no correlation with caries in either stunted or non-stunted toddlers; however, nutritional status (stunting) appeared to influence caries occurrence in toddlers.
Distal root hemisection of mandibular left first molar with pulp necrosis, bifurcation perforation, and symptomatic apical periodontitis
Regina, APrima Vista Okta;
Maharani, Cyntia Dewi;
Widyastuti, Andina;
Rinastiti, Margareta
Majalah Kedokteran Gigi Indonesia Vol 10, No 3 (2024): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada
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DOI: 10.22146/majkedgiind.91794
Hemisection is an endodontic surgery performed by removing one or more roots and existing crown structures to increase the retention of the remaining teeth and to correct defected dental roots that are not possible to maintain. This defect can be caused by pulp tissue or periodontal tissue. Diagnosis of endodontic-periodontal lesions is an essential determinant whether it is due to endodontic or periodontal pathogens. In this case, endodontic treatment needs to be performed in combination with hemisection surgery. The purpose of this case report is to save the tooth with furcation involvement and pulp necrosis, making it perforated on the bifurcation of mandibular first molar. A female patient aged 20 years had a large cavity in tooth 36 with symptomatic apical periodontitis. The tooth was sensitive to percussion test and negative to palpation test, and the mobility was normal. The radiographic feature showed a perforated tooth in the furcation section and furcation involvement. The diagnosis of tooth 36 was pulp necrosis with bifurcation perforation and symptomatic apical periodontitis. The patient received initial treatment, root canal treatment, and obturation on the mesial first molar, then followed by intake of the distal root of the molar. The installation of a prefabricated fiber post in the mesial root of tooth 36 and hemisection surgery were performed on tooth 36. The restoration was done in the form of a full porcelain fused to metal crown on tooth 36 with a splinted crown. Hemisection surgical treatment is a reliable alternative solution and has a good prognosis because it can maintain dental and periodontal tissue as long as possible without tooth extraction.
Oral health attitude and behaviour among dental students
Hariyani, Ninuk;
Alghazaly, Farouk;
Rahmi, Annafsa Zaky;
Qaid, Huda Rashad;
Ahmed, Suha Mudhish Abduljalil;
Silitonga, Friska Miani;
Bayasyut, Muhammad Fahmi;
Shanbhag, Namita
Majalah Kedokteran Gigi Indonesia Vol 10, No 3 (2024): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada
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DOI: 10.22146/majkedgiind.94891
Dental students will play an important role in educating and promoting oral health. The oral health behavior of dental providers and their attitudes toward their oral health could depict the oral health of their patients. The aim of this study was to review the current studies available on the oral health attitudes and behavior of dental students. A systematic search of original articles was carried out in two databases to write this systematic review. MEDLINE (pubMed) and GOOGLE SCHOLAR. Studies published in the English, Arabic and Indonesian languages up to 31/6/2023 was carried out. Search results were managed through EndNote. The search strategy identified 180 articles (133 from Google Scholar and 47 from PubMed databases), of which 22 articles were included in the analysis after the systematic review process. Study of oral health attitude and behavior based on the Hiroshima University Dental Behavioral Inventory (HU-DBI). As a result of variation in the subjects age, measure of outcomes quantitative analysis is not showed. A total of 22 studies met the inclusion criteria. The studies included a total of 7,562 undergraduate dental students and were conducted in 18 countries. The review found that clinical students have good oral health attitudes and behavior compared with preclinical students, showing improvements in oral health attitude and behavior of dental students through the students’ educational progress. The improvement in oral health care has been shown to be linked to experience, especially for clinical students that have been in clinical settings.
Comparison of infection control practices in dental radiographic examination during the COVID-19 pandemic among various health facilities in Yogyakarta, Indonesia
Dewangga, Mella Anggia Puspa;
Shantiningsih, Rurie Ratna;
Widyaningrum, Rini;
Yanuaryska, Ryna Dwi
Majalah Kedokteran Gigi Indonesia Vol 10, No 3 (2024): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada
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DOI: 10.22146/majkedgiind.95895
Dental radiography services were at high risk of becoming sites for cross-infection during the COVID-19 pandemic. To minimize the spread of COVID-19, infection control adaptations were implemented in dental radiography services across various healthcare facilities, including Type A, B, C, and D hospitals, as well as clinical laboratories. This study aims to determine the differences in infection control practices of dental radiographic examinations during the COVID-19 pandemic at various health facilities. This cross-sectional study involved 42 dental radiographers who worked in Yogyakarta. Control infections were measured using the electronic questionnaire with 27 closed-ended questions. The data were analyzed statistically using the Kruskal-Wallis test, and descriptive analysis was continued by categorizing the control infection into high, medium, and low levels. The validity and reliability test showed that 18 questionnaire items were valid and reliable. The statistical test showed a p-value of 0.672 (p > 0.05) for the staff infection control and 0.147 (p > 0.05) for the room infection control. This study found no significant differences in infection control practices during dental radiographic examinations across various healthcare facilities during the COVID-19 pandemic. All facilities implemented high levels of infection control, with clinical laboratories showing the highest compliance. The study suggests increasing the number of questionnaire items related to room infection control and expanding the study’s scope in future research to improve accuracy and represent a broader population.
A relationship between preoperative anxiety and postoperative pain in odontectomy patients under general anesthesia at Moewardi Regional General Hospital
Mursyid, Farhandika;
Septiawan, Debree;
Lisetiawati, Lisetiawati
Majalah Kedokteran Gigi Indonesia Vol 10, No 3 (2024): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada
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DOI: 10.22146/majkedgiind.95909
Odontectomy is a surgical procedure to extract impacted teeth and also commonly performed to address dental and oral health issues. One symptom observed prior to the procedure is anxiety that may cause patients to avoid dental care. One effect attributed to preoperative anxiety in odontectomy patients includes a decrease in pain control, encompassing postoperative pain, possibly through Hypothalamic-Pituitary-Adrenal (HPA) axis. Postoperative pain itself is a complex issue requiring effective management to ensure good healing. This study aimed to determine the relationship between preoperative anxiety and postoperative pain in odontectomy patients under general anesthesia at Moewardi General Hospital and to measure the relationship between preoperative anxiety and postoperative pain intensity in patients undergoing odontectomy under general anesthesia at Moewardi General Hospital. This was a cross-sectional study of which the data were obtained through interviews. A total of 30 samples were selectedfrom the Dental and Oral Outpatient Clinic at Moewardi General Hospital scheduled for odontectomy under general anesthesia from December 2022 to January 2023. The Amsterdam Preoperative Anxiety and Information Scale (APAIS) questionnaire and Visual Analogue Score for Pain were used respectively to measure preoperative anxiety and postoperative pain. Statistical analysis of the relationship was conducted using the Pearson Correlation Test to assess the strength of the relationship. The average preoperative anxiety level was 17.17 and the postoperative pain intensity was 3.93 with the correlation coefficient of 0.587 (p < 0.05), indicating that the relationship fell within moderate and significant category. Preoperative anxiety exhibited a moderate and significant correlation with postoperative pain in odontectomy patients under general anesthesia.
Effect of 10% lemongrass (Cymbopogon citratus) and 3% hydrogen peroxide solution immersion on the dimensional stability of alginate impression
Indriani, Lenni;
Habar, Ike Damayanti;
Sonda, Angelitha Patricia
Majalah Kedokteran Gigi Indonesia Vol 10, No 3 (2024): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada
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DOI: 10.22146/majkedgiind.98582
Pathogenic bacteria can easily spread through impression materials, especially alginate. One of the chemicals disinfection agents used is hydrogen peroxide and natural disinfection alternatives, such as the decoction of lemongrass leaves. The aim of this study is to determine the effect of immersing Alginate impression in a disinfection solution of lemongrass (Cymbopogon citratus) and 3% hydrogen peroxide on the dimensional stability. The samples of this research consisted of 27 alginate impression samples of the upper jaw teeth prepared by manipulating alginate powder with water according to the manufacturer’s ratio and a lemongrass solution prepared by boiling 300 grams of lemongrass leaves 3000 ml of distilled water to obtain 300ml of lemongrass leaf decoction. The 27 samples were divided into three groups: the control group soaked in aquadest, one group soaked in 3% hydrogen peroxide, and one group soaked in 10% lemongrass decoction. The dimensional stability was measured by using a caliper for the horizontal dimension and then analyzed by using the Kruskal-Wallis test. This statistical analysis found a p-value of <0.05 in the horizontal dimension, indicating a difference in the horizontal dimensional stability of the alginate impression after immersion among the hydrogen peroxide, lemongrass leaves (C. citratus), and control groups. Further comparisons among the groups by using the Mann-Whitney Test resulted in a p-value of <0.05 for all the comparisons among the horizontal dimension of the groups. The dimensional changes of the alginate impression were slightly larger in the immersion of 10% lemongrass solution compared to the 3% hydrogen peroxide solution.
Endodontic Care for Patients with Polycythemia: Clinical Considerations and Case Analysis
Nugroho, Dian Lestari Putri;
Nugraheni, Tunjung;
Imandini, Aldila;
Rinastiti, Margareta
Majalah Kedokteran Gigi Indonesia Vol 10, No 3 (2024): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada
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DOI: 10.22146/majkedgiind.91425
Polycythemia is a myeloproliferative neoplasm characterized by uncontrolled proliferation of red blood cells due to mutations in early hematopoietic cells, often linked to Janus kinase 2 (JAK2) gene mutations. Polycythemia vera (PV), the primary form of the disease, leads to increased blood viscosity and stasis, raising the risk of thrombotic events such as stroke and myocardial infarction. Secondary polycythemia results from hypoxic conditions, stimulating erythropoietin production. Diagnosis is confirmed through hemoglobin and hematocrit levels, with treatment aimed at reducing thrombosis and hemorrhage risks. Low-risk patients are treated with aspirin and phlebotomy, while high-risk cases may require cytoreductive therapies like hydroxyurea.In our case, a patient presented with necrosis and horizontal bone loss in tooth 36, complicated by polycythemia. Due to the increased risk of bleeding and inflammation, the decision was made to prioritize endodontic treatment, avoiding periodontal surgery. The patient, considered low-risk for PV complications due to the absence of thrombosis history, was managed conservatively. Preoperative coordination with hematologists was essential to optimize hematologic parameters and minimize potential risks.The goal of endodontic treatment was to disinfect and seal the root canal, reducing inflammation in the surrounding periodontal tissues and preventing reinfection. This approach allowed for effective management of the patient's oral condition while minimizing systemic risks. This case highlights the importance of a multidisciplinary approach in managing dental treatment for patients with polycythemia, ensuring both local and systemic complications are effectively addressed.
Cherubism with peripheral reparative giant cell granuloma in the mandible and maxilla
Sukawijaksa, Hendra;
Arindra, Pingky Krisna;
istadi, Didit;
Haryosuwandito, Erdananda;
Lutfianto, Muhammad Bakhrul
Majalah Kedokteran Gigi Indonesia Vol 10, No 3 (2024): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada
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DOI: 10.22146/majkedgiind.91469
Cherubism is an autosomal dominant disease that causes abnormal osteoblast-osteoclast function, resulting in progressive enlargement around the maxillofacial area due to replacement of normal bone with fibrous tissue and immature bone. The clinical and radiographic signs of cherubism include bilateral radiolucent multi-ocular lesions of the mandible and maxilla, causing bilateral cheek swelling. A 9-year-old boy came to the Oral Surgery of Sardjito Hospital, Yogyakarta, with a complaint of enlargement of both cheeks nine months ago. The patient complained that the swelling on the maxilla enlarged and felt disturbing for his appearance. The enlargement was painless, not easy to bleed, hard, and disturbing when eating and sleeping. Radiograph examination demonstrated bilateral maxillary and mandibular multi-ocular-radiolucent lesions. Three stages of surgery were performed: biopsy-incision with the histopathological result being benign fibrous histiocytoma, curettage-reshaping of the mandible 1 month after biopsy, and curettage-reshaping of the maxilla 9 months after the mandible surgery. The histopathological test of the lesions in the maxilla and mandible showed the same result: peripheral reparative giant cell granuloma. Twelve months postoperatively, the patient had no complaints, showed no disturbances in eating and sleeping, and regained a symmetrical face. The patient's initial curettage and reshaping resolved the masticatory complaints, improved facial aesthetics, and reduced lesions' expansion at the bilateral mandibular and maxillary bones.
Combined techniques of buccal fat pad and buccal advancement flap for revision of failed oroantral fistula closure treatment
Setjadiningrat, Raden Sonny Aditya Santosa;
Istadi, Didit;
Arindra, Pingky Krisna;
Rosanto, Yosaphat Bayu
Majalah Kedokteran Gigi Indonesia Vol 10, No 3 (2024): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada
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DOI: 10.22146/majkedgiind.91493
Oroantral fistula (OAF) is a complication of dental extraction that is defined as open pathological communication between oral cavity and sinus of the maxillary with the involvement of epithelization of a canal. Oroantral fistula will cause infection, impaired healing, and chronic sinusitis. This article reports a case of OAF in tooth 16 which occurred after 11 months following unsuccessful sinus closure treatment. Many approaches are widely used for the closure of OAF, such as using a buccal fat pad (BFP), buccal advancement flap (BAF), or a combination. This study aims to describe the combined techniques of BFP and BAF in OAF closure of large defects with a history of previous closure failure. A 31-year-old male patient came to our hospital for the presence of OAF for 2-3 weeks. The patient underwent OAC closure at another hospital 11 months prior, but the complaint recurred. Diagnosis of OAF was carried out using the Valsalva test. A combination of BFP and BAF techniques was done to close the OAF after the removal of the epithelial. Evaluation of the treatment after 30 days showed adequate healing and full closure of OAF. OAF closure with the combined techniques of BFP and BAF displays promising results for the revision of failed OAF closure treatment.