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Sitotoksisitas Ekstrak Aspergillus fumigatus dari Daun Mekai (Albertisia papuana Becc.) terhadap Sel Kanker Payudara T47D dan MCF-7 Hasnaul Maritsa; Soekarti Moeljopawiro; Rina Sri Kasiamdari
BIO-SITE |Biologi dan Sains Terapan Vol. 1 No. 1 (2015): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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The previous studies showed that the Albertisia papuana Becc. root have cytotoxicity on breast cancer. The A. papuana root toxicity on breast cancer could not only by plant secondary metabolism, but may be also by secondary metabolism of endophytes. Aspergillus fumigatus is one of endophytes that have anticancer agent. Endophytes can be distributed dynamically in whole of plant organ, one of them are leaves. Therefore the objective of this studies were to know the presence of A. fumigatus in A. papuana leaves, and the cytotoxicity of their secondary metabolism on breast cancer cells. The sample of A. papuana were collected from Botanical Zoo of Bogor, while T47D and MCF-7 cell lines were obtained of Tropical Medicine’s Faculty, UGM. Isolation of endophytes was done by growing leaves extract on water agar 2 % medium. Secondary metabolism was extracted from fermented broth using in ethyl acetat and n-butanol. The cytotoxicity was perform by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The result showed that A. fumigatus assosiated with A. papuana leaves. Ethyl acetat extract from fermented A. fumigatus both on T47D and MCF-7 cell lines had lower (IC50. 50, 444 µg/ml and 59 µg/ml) than n-butanol (IC50. 103, 398 µg/ml and 127,188 µg/ml. It could be said that A. fumigatus from A. papuana leaves could induce cytotoxicity on T47D and MCF-7 breast cancer cells.

Variasi Karakter Kuantitatif Kepiting Uca annulipes (Brachyura : Ocypodidae) di Kepulauan Indonesia Dewi Citra Murniati
BIO-SITE |Biologi dan Sains Terapan Vol. 1 No. 1 (2015): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Uca annulipes are deposit feeder crab which is widely distributed in Indonesian mangrove areas. The systematic study of the species completely based on the qualitative character without the quantitative one. This study describes the variation in quantitative characters of U. annulipes from different locations in Indonesia. Descriptive method with univariate and multivariate analyses based on quantitative character ratio was used in this study. The univariate analysis showed that the highest value of either the quantitative character or quantitative character ratio was found from the western Indonesian population. Meanwhile, the multivariate analysis showed that U. annulipes from western Indonesia, Sulawesi, and Nusa Tenggara have a discrete cluster.

Bioaktivitas Ekstrak N Heksan Umbi Ubi Jalar AC Kuningan (Ipomoea batatas) Dalam Menghambat Pertumbuhan Beberapa Patogen Dan Pemanfaatannya Untuk Fortifikasi Makanan Ilah Nurlaelah
BIO-SITE |Biologi dan Sains Terapan Vol. 2 No. 1 (2016): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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AC kuningan sweet potato (Ipomea batatas) is one of the endemic plants in Kuningan district which became the biggest commodity. Sweet potato tuber is a food rich in vitamins and minerals that include beta carotene, polyphenols, vitamin B1, vitamin C, calcium, phosphorus, iron, potassium and sodium. This study aimed to obtain the best concentration of n-hexane extract of AC Kuningan sweet potato tubers (Ipomea batatas) in inhibiting the growth of some pathogens from the type of potato AC red and white AC Kuningan tested against microbes in the form of selected bacteria comprising E. coli, Bacillus sp and Staphylococcus sp collection of FKIP UNIKU biology laboratory, determined the content of polyphenols and antioxidant activity as well as the potential for the best formula in the sweet potato flour fortified biscuits. The method used was experimental laboratory. Data on antibacterial bioactivity was measured by paper disc diffusion method, analysed chemical compounds through spectrophotometer polyphenols and antioxidant activity with DPPH method. Analysis of the data was used for testing antibacterial that was ANOVA test. Determining the level of preference for the value of biscuit products was using hedonic test and Freadment. The results showed that the highest inhibitory potency extract of n-hexane potato red AC shown at a concentration of 2000 ppm n-hexane while to extract the white yam AC shown at a concentration of 500 ppm. Both types of potato AC Brass had fairly high polyphenol content for sweet potatoes 356.78 ± 19.25 ppm and white potatoes 206.72 ± 6.90 ppm and antioxidant activity with IC50 values are shown respectively in figures 1759.89 ± 1793.60 ± 7.18 ppm and 25.41 ppm was beneficial to fight free radicals. Test formulation 1 ranking indicated that it wasmore desirable biscuits by panelists in terms of taste, texture, color and aroma. It showed sweet potato flour helpful for fortifying foods that had nutritional value and better flavor.

Efektivitas Pelaksanaan Rencana Pengelolaan Lingkungan dan Rencana Pemantauan Lingkungan (RKL-RPL) Pertambangan Batubara PT. Adaro Indonesia di Kabupaten Tabalong, Provinsi Kalimantan Selatan Iid Moh Abdul Wahid; Budhi Gunawan; Teguh Husodo
BIO-SITE |Biologi dan Sains Terapan Vol. 2 No. 1 (2016): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Environmental Impact Assessment (EIA) is a planning and preventive document. This is one of the environmental protection and management efforts in order to prevent environmental damage. In Tabalong there are more than 30 business agent and / or activities that have made EIA mining, 24 of which are mining operations. PT Adaro Indonesia is the largest company in Tabalong with a concession area of 35,536 hectares and a production capacity of 80 million tons / year and has had environmental documents (EIA) and Environmental Permit. During this time PT. Adaro Indonesia, in operation, has been carrying out management and environmental monitoring (RKL-RPL) in the area of mining, but on the other hand the number of public complaints due to alleged pollution and / or destruction of the environment by the activity of PT. Adaro Indonesia remains the case that many questioned the implementation of its EIA (RKL-RPL). This research aims to study the effectiveness and the factors that affect the implementation of the EIA (RKL-RPL) coal mining PT. Adaro Indonesia. This research was conducted using qualitative-quantitative approach (mix method) with concurrent triangulation strategy model. The research data obtained through observation, interviews, questionnaires and review of the literature. The results showed that the implementation of the RKL-RPL PT. Adaro Indonesia is said to be effective with each aspect of obedience (compliance) 95%, institutional 91.67%, Monitoring (supervision) 92.86% and handling public complaints66.67%. The factors that influence the effectiveness of the implementation of the EIA PT. Adaro Indonesia is communication and coordination, resources (staff, skills, information, authority, and facilities), regulation and government policy, funding, enforcement and legal certainty (incentives / disincentives), and bureaucratic institutional structure.

Identifikasi Bakteri Tanah di Kebun Botani Biologi FKIP Universitas Jambi Okti Chairani; Retni S Budiarti; Winda Dwi Kartika
BIO-SITE |Biologi dan Sains Terapan Vol. 2 No. 1 (2016): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Soil bacteria has important role in the life cycle of an ecosystem. Their life activities affect the characteristics of the biologic, physical, and chemical environment. Biology study program of education faculty in Jambi University has a plot of land made into a botanical garden. Thus, this botanical garden needs some information about the fertility of the land used, and one of them is to know the type of bacteria in its soil as a biological factor. The purpose of this research is to determine the genus of bacteria found in soil of Biology Botanical Garden at Education Faculty (FKIP) of Jambi University, Mendalo. This research was conducted from April to July of 2015, in both biology botanical garden and biology laboratory at Education Faculty of Jambi University. The kind of this research is explorative descriptive. Soil sample was collected by using 4 squared plots measuring 12 m x 12 m. In each plot made 5 subplots and soil samples was taken from 3 points of each subplot by random sampling. The identification was done by observing colony morphology, gram staining, and biochemical tests. The result of this research was gained gained 9 genus of bacteria, they were Paracoccus, Nitrosococcus, Bacillus, Cellulomonas, Acinetobacter, Micrococcus, Pseudomonas, Azotobacter, and Zymomonas. It is suggested to further researchers to study about other microorganisms in the soil Biology Botanical Garden that have role in maintaining and improving soil fertility there.

Isolasi dan Identifikasi Jamur Potensial Pendegradasi Selulosa pada Limbah Pelepah Kelapa Sawit D\di Daerah Kabupaten Rokan Hulu, Riau Filza Yulina Ade
BIO-SITE |Biologi dan Sains Terapan Vol. 2 No. 1 (2016): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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In plant there are the cellulose component composition pretty much. Usually there are some microorganisms that can degrade cellulose. A research about isolation and identification of fungus degradation of cellulose on waste palm fronds, had been done using purposive sampling method. This research aimed to look the type of fungus species from waste palm fronds which have the potential to degrade of cellulose and to know can be able in cellulose degradation. Result showed that species of fungus have cellulose degradation potential. Kinds of fungus isolate which founded are Aspergillus niger, Aspergillus oyzae and Rhizopus oryzae. All the fungus isolate have good and big potential in cellulose degradation with afforded to starch degrade at medium to fast rate.

Rotifera pada Area Bekas Tambang Emas di Kabupaten Sawahlunto Sijunjung Silvi Susanti; Abizar Abizar
BIO-SITE |Biologi dan Sains Terapan Vol. 2 No. 1 (2016): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Rotifers is one of many zooplankton commonly found in freshwater. As the heterotrophic animal rotifers were able to recycle organic material so that the rotifers could live in mined areas or kolong. An abundance of rotifers and chemical physics factor measurements taken at six stations using the method descriptive survey conducted to determine the relationship with kolong’s age. The results showed that the station over the age of 12 months have been located in waters climactic conditions.

Studi Zooplankton di Danau Teluk Kota Jambi Mahya Ihsan; Ummi Mardhiah Batubara; Hesti Riany; Ika Oksi Susilawati; Teguh Sumarsono
BIO-SITE |Biologi dan Sains Terapan Vol. 2 No. 1 (2016): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Lake Teluk natural potential is very rich in zooplankton. However, the there are many untapped potential, so that appropriate unknown environmental factors to support the growth, reproduction, and abundance of zooplankton that is potential as fish food can be cultivated properly. Therefore, there needs to be research related to environmental factors and abundance of zooplankton in Lake Teluk Jambi city in order to see the natural potential of fish forage in the area. This study was conducted at three stations in the Lake Teluk Jambi and Biotechnology Laboratory, Faculty of Science and Technology, the Jambi University. The method used in this research was the survery and analysis. Measured parameters were factors abundance of zooplankton and the physical and chemical environmental factors. The identification results obtained three phyla of zooplankton (protozoa, rotifers and sponges) and 14 genuses that were Platytrichotus, Centropyxis,Perispira, Corythion, Acropisthium, Turania, Trinema, Euglypha, Enteroplea, Proalinopsis, Pedipartia, Trocospheera, Filinia, Meyenia. Analysis of physical and chemical environmental factors showed solubility values (DO) range 4,8 - 6,8 mg/L, the water temperature ranges from 29-30 °C and oxygen consumption (BOD) in the range of 3,7 to 6,6 mg/L relatively optimal for the growth of zooplankton, but have not found any zooplankton that have the potential to be cultivated as a natural fish feed.

Uji Aktivitas Antibakteri Ekstrak Etanol Biji Ganitri (Elaeocarpus sphaericus Schum.) terhadap Pertumbuhan Bakteri Penyebab Demam Typhoid secara In vitro”. Dewi Maesyaroh; Ega Bramaseta Imansyah; Hilda Febiana Nafratilova; Nimas Putri Anggarani; Sunawan Sunawan
BIO-SITE |Biologi dan Sains Terapan Vol. 3 No. 2 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Abstrak Penelitian yang dilakukan di Laboratorium Biologi Universitas PGRI Banyuwangi ini, bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.) dalam menghambat pertumbuhan bakteri penyebab demam typhoid secara in vitro dan untuk mengetahui Konsentrasi Hambat Minimum (KHM) ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.). Metode yang digunakan ialah metode sumuran dengan control positif tetrasiklin 1% dan control negative aquades steril. Serial konsentrasi ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.) yang digunakan sebesar 10%, 20%, 30%, 40%, dan 50%, dan pada uji KHM menggunakan serial konsentrasi sebesar 1%, 2,5%, 5%, 7,5%, dan 10%. Berdasarkan hasil uji, zona hambat terbesar ditunjukkan oleh konsentrasi 50% dengan rata-rata diameter zona hambat sebesar 1.030 cm dan zona hambat terkecil ditunjukkan konsentrasi 10% dengan rata-rata diameter zona hambat sebesar 0.203 cm. Hasil uji ANOVA nilai (F.Hit>F. Tabel) dengan F.Hit sebesar 17.638 dan F.Tabel sebesar 2.85 serta nilai signifikasi sebesar 0.000 (P<0.05), karena nilai P<0.05 maka dapat disimpulkan bahwa terdapat Pengaruh konsentrasi ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.) terhadap pertumbuhan bakteri uji. Hasil uji Duncan menunjukkan ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.), pada konsentrasi 50% mempunyai zona hambat yang berbeda nyata atau berbeda signifikan terhadap konsentrasi perlakuan 10%, 20%, 30%, kontrol negatif dan kontrol positif, namun tidak berbeda nyata dengan konsentrasi 40%. Sedangkan KHM ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.) yang masih mampu menghambat pertumbuhan bakteri uji pada konsentrasi 1% dengan rata-rata diameter zona hambat sebesar 0.037 cm.

Pertumbuhan Tanaman Sawi Hijau (Brassica juncea L.) dengan Pemberian Kompos Berbahan Dasar Daun Paitan (Thitonia diversifolia) Istarofah Istarofah; Zuchrotus Salamah
BIO-SITE |Biologi dan Sains Terapan Vol. 3 No. 1 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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This study aimed to determine the growth of mustard green (Brassica juncea L.) by addion Paitan leaves (Tithonia diversifolia) based compost at different doses and to determine the optimum dose of compost. This study used a completely randomized design (CRD) with one factor that is the dose of Paitan leaves based compost and the treatments were: A1 = 0.25 kg of compost, A2 = 0.5 kg of compost, A3 = 0.75 kg of compost, A4 = 1 kg of compost, A5 = 1.25 kg of compost and control (without compost), and each treatment was added by the soil so that the total weight was 2.5 kg in each polybag. Observation parameters were the plant height, leaf number, leaf length, leaf width, wet weight and dry weight of plants. The data were tested with Analysis of Variant (ANOVA) and followed by LSD test at 5% level. Based on the results of this study, it was concluded that the growth of mustard green plant by addition Paitan leaves based compost at various doses showed different growth of plants. the A3 Treatment is the most optimum dose to raise the growth of mustard green.

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Kota jambi,

Jambi

INDONESIA