Garuda - Garba Rujukan Digital

Isolation of Fungal Endophytes from Melastoma malabathricum L. and Their Potential as Antifungal Rusi Octavianti; Israwati harahap; Elsie Elsie
BIO-SITE |Biologi dan Sains Terapan Vol. 3 No. 1 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (441.976 KB)

The aim of this study was to isolation the endophytic fungi from medicinal plant Melastoma malabathricum L. and to investigate their potential antifungal activity. Isolation endophytic fungi was carried out using surface sterilized method. The fungal extracts were assessed for antifungal activity against Candida albicans using agar well diffusion method. A total of 28 isolates fungal endophytes from leaves, stems, flowers and roots of Melastoma malabathricum L. The results showed that of twelev have strong category within inhibit growth of C. albicans. Isolate 18 is endophytic fungi who produced the largest zones of inhibit is 19 mm.

Formulasi Media Produksi Bibit F2 Jamur Tiram Putih Ika Oksi Susilawati; Witiyasti Imaningsih; Arif Mulyanto
BIO-SITE |Biologi dan Sains Terapan Vol. 3 No. 1 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.402 KB)

White oyster mushroom (Pleurotus ostreatus L.) is a fungus that consumption demand by people Banjarbaru. However, the fulfillment of this fungus is still lacking, has always supplied from Java. Based on these problems, it is necessary to study the composition of the growth media F2 seeds oyster mushroom. The purpose of this study was to determine the composition ratio of constituents of seedling growth media appropriate for the growth of white oyster mushroom seeds. This research was conducted by dividing some compositions media. Comparison of composition constituents of the right media is used to make seedling growth media F2 is a mixture of sawdust with grain (50%: 50%). In addition the use of pure sawdust can also be used as a media for the manufacture of white oyster mushroom F2 seeds. The composition of the media mix sawdust with grain (50%: 50%) effectively used to create F2 seed oyster mushroom.

Keragaman Lumut Epifit di Hutan Kota dan Tepi Jalan Utama Kampus Universitas Indonesia Afiatri Putrika; Nisyawati Nisyawati; Nunik Sri Ariyanti
BIO-SITE |Biologi dan Sains Terapan Vol. 3 No. 1 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (801.158 KB)

Research on epiphytic bryophytes has been conducted in two different sites located in Universitas Indonesia (UI). Those sites were urban forest and vegetation on main street margin of the campus. This study was carried out to compare diversity of the bryophyte at both sites. Twelve plots of 25 x 25 m2 were establish at the forest, while nine of 50 m line transect were made at the street margin. Five trees of each plot or line transect were sampled. Eight sub plots of 15 x 15 cm2 were placed on each trunk base (0--200 cm) of the tree sampels. The results obtained 23 species of epiphytic bryophytes, 21 species occured in the forest and 14 species were found at street margin. The similarity of bryophyte community between the forest and street margin were high (Sorenson similarity index = 0.73). Octoblepharum albidum was the dominant species at the forest, while Calymperes tenerum was dominant at the street margin. The diversity of epiphyte bryophyte at both sites were categorized low based on Shannon Wiener index (H’< 2), however they were not different significantly.

Efektivitas Bakteri Amilolitik Asal Geopark Merangin Jambi terhadap Patogenitas Jamur Pyricularia oryzae Penyebab Penyakit Blas Daun Padi Ummi Mardhiah Batubara; Suparjo Suparjo; Hasnaul Maritsa; Nanda Fahmuin Tari; Selvi Andriani
BIO-SITE |Biologi dan Sains Terapan Vol. 3 No. 1 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.05 KB)

Rice (Oryza sativa) is a major crop product in Asia. Rice products are increasing along with the increasing of human population. Blast disease in leaf (leaf blast) is the major pathogen in rice during vegetative phase. The using of amylolytic bacteria isolated from Geopark Merangin Jambi is done as an attempt to discover biocontrol agents in order to attack infection of Pyricularia oryzae, the cause of leaf blast disease. Antimicrobial activity assay was carried out by isolating the pathogen in the infected rice crops. Pyricularia oryzae that had been cultured then were evaluated their antagonistic activity against amylolytic bacteria isolated from prior experiment. Results have shown that from 25 isolates of amylolytic bacteria taken from Geopark Merangin Jambi, 2 isolates that can be used as biocontrol agents were GM20 and GM23 capable of inhibiting the growth of Pyricularia oryzae.

Asosiasi Cerbera manghas pada Komunitas Tumbuhan Bawah di Areal Hijau Universitas Jambi Mahya Ihsan
BIO-SITE |Biologi dan Sains Terapan Vol. 3 No. 1 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (366.154 KB)

Berbagai jenis tumbuhan dalam komunitasnya cenderung hidup berdampingan dengan tumbuhan lain baik yang sejenis maupun yang berbeda jenis. Hubungan ketertarikan untuk tumbuh bersama pada tumbuhan dikenal dengan sebutan asosiasi. Salah satunya komunitas tumbuhan di Universitas Jambi didominansi oleh komunitas tumbuhan Cerbera manghas. Tumbuhan ini tumbuh menyebar di seluruh areal kampus. Berdasarkan hasil analisis, dari 24 spesies yang pada plot pengamatan tumbuhan yang memiliki INP ≥ 10% hanya 6 tumbuhan, yaitu Axonopus sp. , Albizia sp., Tinospora sp., Paspalum sp., Gleichenia sp. dan Lygodium sp. Sedangkan nilai indeks asosiasi IO, tingkat kekuatan asosiasi yang terbesar dimiliki oleh Albizia sp., Gleichenia sp. dan Lygodium sp. Hal tersebut menunjukkan ketiga tanaman tersebut memiliki hubungan asosiasi dengan tumbuhan Cerbera manghas yang ditunjukan dengan nilai Indeks Ochiai mendekati 1.

Isolasi dan Identifikasi Cemaran Bakteri Salmonella sp. pada Daging Ayam dan Ikan Mentah Hasna Ul Maritsa; Fitratul Aini; Ardiansyah Saputra; Desri Santi Nurhakim; Greace Meisinta Sihombing
BIO-SITE |Biologi dan Sains Terapan Vol. 3 No. 2 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Food is a basic need that must be free from microorganisms contamination Salmonella sp. is a gram-negative bacteria that often contaminates food, especially meat, and causes Salmonellosis disease. This study aims to isolate and identify Salmonella sp. which allegedly contaminating the sample of chicken meat and raw fish. Samples from traditional market around Jambi University. The tests were conducted at the Laboratory of Biotechnology and Engineering, Faculty of Science and Technology, Jambi University. Isolation was performed by dilution method on SSA medium (Salmonella Shigella Agar), isolates were characterized by macroscopic-microscopic morphology, and biochemical test. The results showed that raw chicken meat was positively contaminated with Salmonella sp., while fish meat was not. Gram-negative bacillus isolate character is yields H2S black deposits and is capable of fermenting glucose, lactose and sucrose.

Kajian Tanaman Penyerap Timbal (Pb) dan Pengikat Karbon di Lingkungan Kampus Universitas Jambi Fitratul Aini; Siti Mardiyah; Fitri Wahyuni; Aulia Ul Millah; Mahya Ihsan
BIO-SITE |Biologi dan Sains Terapan Vol. 3 No. 2 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Kampus Universitas Jambi di daerah Mendalo memiliki jumlah mahasiswa terbanyak dibandingkan dengan kampus UNJA yang lain, dan sebagian besar mahasiswa menggunakan kendaraan bermotor. Kampus ini juga berada di jalan lintas sumatera yang dilalui oleh banyak kendaraan yang menghasilkan asap yang mempengaruhi polusi udara di sekitar kampus. Berbagai jenis tanaman diyakini dapat menyerap karbon dan timbal (Pb) sehingga mampu mengurangi dampak polusi. Untuk itu perlu dilakukan penelitian untuk mengetahui tanaman yang efektif menyerapan timbal (Pb) dan mengikat karbon pada beberapa tanaman di Kampus Universitas Jambi, Mendalo. Penelitian ini dilaksanakan selama 6 bulan di Laboratorium Bioteknologi dan Rekayasa, FST UNJA dan Laboratorium Terpadu UNPAD Bandung. Metode dalam penelitian ini menggunakan metode survei untuk menentukan lokasi penelitian yaitu gerbang utama (stasiun I), gerbang ATM (Stasiun II), area rektorat (Stasiun III), area fakultas ekonomi (Stasiun IV), dan area FST serta kehutanan (Stasiun V). Data yang dikumpulkan berupa kadar timbal (Pb) dan karbon. Hasil penelitian menunjukkan bahwa tanaman yang mampu menyerap kadar timbal terbesar adalah bintaro sedangkan tanaman penyerap karbon terbaik adalah mahoni. Besarnya kadar timbal dan karbon tanaman dipengaruhi beberapa faktor diantaranya lokasi pengambilan sampel, intensitas kendaraan, umur daun tanaman, dan jenis tanaman

Biologi Reproduksi dan Polinasi Buatan Tumbuhan Senduduk (Melastoma malabathricum L.) Lilis Suryani
BIO-SITE |Biologi dan Sains Terapan Vol. 3 No. 2 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Abstract Melastoma malabthricum is the type with the widest area of spread compared with other species in the genus Melastoma. This research uses floral phenology observation method and pollination experiment by using bagging technique, emasculation and artificial pollination. Stigma receptivity was tested according to the Matson method. The results showed that M. malabathricum’s flowers take 14 until 21 days from bud to flower bloom. Stigma M. malabathricum is already present and receptive before anthesis flower. Fruit can be formed through self pollination (autogamy) and cross pollination (xenogamy). M. malabathricum pollination approaches the xenogamy facultative.

Pengaruh Pemberian Bokashi Campuran Alang-alang (Imperata cylindrica L.) dan Kotoran Kambing Terhadap Pertumbuhan Tanaman Sawi (Brassica rapa L.) Susilo Susilo; Liza Verizza Triansyah; Maryanti Setyaningsih
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian bokashi campuran alang-alang (Imperata cylindrica L.) dan kotoran kambing terhadap pertumbuhan tanaman sawi (Brassica rapa L.). Metode penelitian yang digunakan adalah metode eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL), yang terdiri dari 5 perlakuan dengan 5 ulangan. Parameter pertumbuhan yang diamati dalam penelitian ini meliputi berat segar, panjang akar dan berat kering tanaman. Hasil penelitian menunjukkan bahwa perlakuan P2 (dosis 20 g) mendapatkan nilai rata-rata terbaik untuk berat segar dan berat kering yaitu secara berturut-turut 30,692 g dan 1,680 g, dengan nilai persentase yaitu 301,49% dan 390,70% terhadap kontrol. Sedangkan pada parameter panjang akar tanaman hasil nilai rata-rata paling baik terdapat pada P3 yaitu 5,46 cm dengan persentase 177,27% terhadap kontrol. Berdasarkan uji statistik yang dihitung menggunakan uji ANAVA 1 Faktor, pertumbuhan tanaman sawi pada parameter berat segar, panjang akar dan berat kering tanaman yang secara berturut-turut menunjukkan bahwa Fhitung ≥ Ftabel, yaitu 3,40 > 2,87; 2,87 ≥ 2,87 dan 4,86 > 4,43. Oleh karena Fhitung ≥ Ftabel, maka kelima perlakuan dinyatakan berpengaruh signifikan terhadap parameter berat segar dan panjang akar tanaman dan berpengaruh sangat signifikan pada parameter berat kering tanaman. Berdasarkan hasil tersebut dapat disimpulkan bahwa bokashi campuran alang-alang (Imperata cylindrica L.) dan kotoran kambing berpengaruh terhadap pertumbuhan tanaman sawi (Brassica rapa L.).

Deteksi Cemaran Bakteri Escherichia coli dalam Jaruk Tigaron pada Pasar Sungai Andai dan Pasar Lama Kota Banjarmasin Ika Oksi Susilawati; Rini Saidah
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Jaruk tigaron is one of the traditional fermented foods originating from South Kalimantan made by fermented tigaron flowers (Crataeva nurvala, Buch HAM). The process of making traditionally allows contamination by the bacteria Eschericia coli (E.coli). Escherichia coli is the most commonly used a microbe as an indicator of faecal contamination in water, even food or beverages, including various types of jaruk. This final project aims to detect Escherichia coli on jaruk tigaron thatdistributed in Banjarmasin. The method was used in detecting Escherichia coli is the Most Probable Number (MPN) with five test stages are estimation test, confirmatory test, complementary test, biochemical test (TSIA and IMVIC). The media was used is a Lactose Broth Single Strength (LBSS) by 3 series for forecasting test, Brilliant Green Bile Broth (BGLB) media for confirmatory test, Eosin Methylene Blue Agar (EMBA) media for complementary test, TSIA test and IMVIC test. The test results was obtained by MPN index on samples (a) 28 samples (b)> 1.100 and sample (c) 20. The detection results is Escherichia coli on jaruk tigaron thatdistributed in Banjarmasin declared negatively containing Escherichia coli but only contain Coliform bacteria. Based on SNI 7388: 2009, the determination of the maximum limit of microbial contamination in food is the absence of Coliform and Escherichia coli bacteria in 100 ml of sample

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota jambi,

Jambi

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official -

Editorial Address -

Location Kota jambi, Jambi INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota jambi,

Jambi

INDONESIA