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BIO-SITE |BIOLOGI Sains Terapan
Published by Universitas Jambi
ISSN : -     EISSN : 25026178     DOI : -
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
Arjuna Subject : -
Articles 51 Documents
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DIVERSITY AND POTENTIAL OF MEGACHIROPTERA BAT AS DISPERSAL AND POLINATOR AT HARAPAN RAINFOREST, JAMBI: DIVERSITAS DAN POTENSI KELELAWAR MEGACHIROPTERA SEBAGAI DISPERSER DAN POLINATOR DI HUTAN HARAPAN, JAMBI fitria ramona
BIO-SITE |Biologi dan Sains Terapan Vol. 5 No. 01 (2019): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.112 KB) | DOI: 10.22437/bs.v5i01.7074

Abstract

Abstract Diversity and potential of Megachiroptera bat as dispersal and polinator was conducted to know diversity of Megachiroptera bats at Harapan Rainforest, potential of Megachiroptera bats as dispersal seed and pollinator and to know the daily activity of Megachiroptera bats pollinate and disperse the seeds of plants in Harapan Raiforest, Jambi . We used purposive sampling method using mist net were installed 90 traps – night. The result showed that five species of Megachiroptera bat are Cynopterus brachyotis , C. horsfieldi , C. sphinx , C. titthaecheilus and Macroglossus minimus with index of diversity value and evenness index are relatively low about 0.99 and 0.62. Kinds of Megachiroptera bats potentially as dispersal are C. brachyotis , C. horsfieldi , C. sphinx , and C. titthaecheilus and bats potentially as pollinator are C. brachyotis , C. sphinx and M. minimus. The pattern of time daily activity of Megachiroptera bats as disperse seeds and pollinate plants in Harapan Rainforest by C. sphinx and C. brachyotis was whole of night with peak activity C. sphinx at night ( 21:00 pm to 00:00 am) and C. brachyotis at end of night ( 3:00-6:00 am ). Key word : Megachiroptera bat, dispersal, polinnator, Harapan Rainforest
Isolasi dan Identifikasi Jamur Patogen pada Tanaman Nanas (Ananas comosus (L). Merr) Varietas Tangkit Selvi andriani; Fitratul Aini; Mahya Ihsan
BIO-SITE |Biologi dan Sains Terapan Vol. 5 No. 01 (2019): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/bs.v5i01.6579

Abstract

ABSTRACT Pineapple (Ananas comosus L. Merr) is a type of tropical fruits that is potential to be developed into trade commodity in Indonesia. Due to its strategic geographical condition and fertile type of soil to support their growth, the majority of Tangkit Baru villager utilize their land to cultivate the pineapples. One of the contributing factors that hinders the cultivation process is the presence of plant pest organisms that can interfere with the growth of the fruit and affect its production. The aim of this study is to determine the types of pathogenic fungi and symptoms of diseases caused by pathogenic fungi in pineapple farm in Tangkit Baru village. This study consisted of two steps: isolation and identification. Based on the result of the isolation and identification steps of pathogenic fungi in pineapple, it was found that there were three fungal isolates namely BD 01, BD 02 and LYA. BD 01 isolate was a type of fungus coming from the genus Penicillium which caused regular leaf spots, while the genus of BD 02 isolate was Phoma, which caused irregular leaf spots and LYA isolate’s genus was Trichocladium which caused wilt. Keyword : Pathogen fungi, Pineapple, Tangkit Baru Village Nanas (Ananas comosus (L). Merr) merupakan salah satu jenis buah tropis yang memiliki potensi untuk dikembangkan menjadi komoditas perdagangan di Indonesia. Kondisi geografi dan jenis tanah yang mendukung pertumbuhan nanas, menjadikan sebagian besar masyarakat di Desa Tangkit Baru memanfaatkan lahannya untuk membudidaya tanaman nanas. Salah satu faktor yang menjadi kendala dalam proses budidaya adalah adanya organisme pengganggu tanaman (OPT) yang dapat mengganggu pertumbuhan dan mempengaruhi hasil produksi nanas. Tujuan dari penelitian ini untuk mengetahui jenis-jenis jamur patogen dan gejala penyakit yang disebabkan oleh jamur patogen pada tanaman nanas di Desa Tangkit Baru. Penelitian ini terdiri dari dua tahap yaitu isolasi dan identifikasi. Tahap isolasi jamur patogen meliputi pengamatan gejala penyakit, penyiapan dan pembuatan media, pengukuran faktor lingkungan dan persiapan isolasi, dan isolasi. Tahap identifikasi meliputi pemurnian, karakterisasi dan identifikasi jamur patogen secara makroskopis dan mikroskopis. Hasil isolasi dan identifikasi jamur patogen pada nanas ditemukan lima isolat jamur yaitu BD 01A, BD 01B, BD 02A, BD 02B dan LYA. Isolat BD 01A dan BD 01B merupakan jamur dari genus Penicillium yang menyebabkan gejala penyakit berupa bercak daun beraturan, isolat BD 02A dan BD 02B merupakan jamur dari genus Phoma yang menyebabkan gejala penyakit berupa bercak daun tidak beraturan dan isolat LYA merupakan jamur dari genus Trichocladium yang menyebabkan gejala penyakit berupa layu. Kata Kunci: Fungi pathogen, nenas, desa Tangkit Baru
Isolasi dan Karakterisasi Bakteri Termofilik Penghasil Inulinase dari Sumber Air Panas Husnin Nahry Yarza; Yuni Ahda; Mades Fifendi
BIO-SITE |Biologi dan Sains Terapan Vol. 6 No. 01 (2021): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Bakteri termofilik penghasil inulinase merupakan bakteri yang dapat menghasilkan enzim inulinase yang tahan pada suhu tinggi.. Penelitian ini bertujuan untuk mengisolasi bakteri termofilik penghasil inulinase dari sumber air panas Padang Ganting Kabupaten Tanah Datar dan mengetahui karakteristik bakteri penghasil inulinase dari sumber air panas. Prosedur penelitian ini dilakukan beberapa tahap yaitu isolasi bakteri dari sumber air panas dengan menggunakan sampel air. Bakteri yang didapatkan dimurnikan sampai dihasilkan koloni tunggal, dan dilakukan pewarnaan gram dari isolat bakteri. Pada penelitian ini diperoleh empat isolat koloni bakteri termofilik penghasil inulinase. Hasil identifikasi secara morfologi menunjukkan bahwa Isolat koloni 1(IKA 1) berbentuk rizoid, permukaan halus, dan warna coklat. Isolat koloni 2 (IKA 2) berbentuk filamen, permukaan mengkilat dan warna coklat. Isolat koloni 3 (IKA 3) berbentuk filamen, permukaan halus, elevasi datar dan berwarna coklat. Isolat koloni 4 (IKA 4) berbentuk bulat, tepi bergelombang, permukaan halus, elevasi datar dan warna coklat. Hasil identifikasi secara mikroskopis (pewarnaan gram) pada keempat isolat koloni tergolong gram negatif dan sel berbentuk batang (bacillus). Kesimpulan adalah ditemukan 4 isolat koloni bakteri termofilik penghasil inulinase dari sumber air panas Padang Ganting Kabupaten Tanah Datar. Keywords; seleksi, karakterisasi, bakteri termofilik, enzim, inulinase Inulinase producing thermophilic bacteria is a bacteria that can produce a thermostable inulinase enzyme. This study aimed to isolate the inulinase-producing thermophilic bacteria from hot springs Padang Ganting Kabupaten Tanah Datar and knowing the characteristics of inulinase producing thermophilic bacteria from hot springs Padang Ganting Kabupaten Tanah Datar. The procedure is done several steps of research is the isolation bacteria from hot springs Padang Ganting Kabupaten Tanah Datar using water samples. Bacteria acquired up to purified single colonies, staining gram isolate bacterial. In this research get it five isolates inulinase producing thermophilic bacteria. The results of the morphological identification showed that isolates 1 (IKA 1) a rizoid shaped, surface smooth and color of chocolate. Isolates 2 (IKA 2) filament-shaped, shiny surface and color brown . Isolates 3 (IKA 3) filament-shaped, surfaces smooth, elevation and flat brown. Isolate 4 (IKA 4) is round, wavy edges, smooth surface, flat elevation and brown. The results of identified microscopically (gram stain) in all five isolates were classified as gram negative. The conclusion was found 4 isolates of inulinase-producing thermophilic bacterial colonies from hot springs Padang Ganting Kabupaten Tanah Datar. Keywords: selection, characterization, thermophilic bacteria, enzyme, inulinase
Akumulasi Mn dalam Cairan Tubuh Ligumia nasuta (Say, 1817) di Sungai Ciputri Bandung Jawa Barat Endah Kartikawati; Andhika Puspito Nugroho
BIO-SITE |Biologi dan Sains Terapan Vol. 2 No. 2 (2016): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Ciputri upriver, Bandung, West Java has polluted by heavy metal Mn coming from cow feces in the Cikidang village. The aim of research is to study Mn accumulation in the Eastern Pondmussel Ligumia nasuta (Say, 1817). Mn contents in the water, extra pallial fluid, and hemolymph were determined by Atomic Absorbance Spectrophotometer (AAS). The mussels were collected at day 0, 1, and 6 after transplantation at 3 stations: before, near, and after pollution sources. The results show that the highest Mn accumulation was in the extra pallial fluid (1,21 mmol kg-1 ww) at station 2, day 6. It showed that exposure of Mn to the mussels taken place via water.
Kajian Etnozoologi Hewan yang Dikonsumsi pada Komunitas Orang Rimba di Taman Nasional Bukit Duabelas Kabupaten Sarolangun Masyithah Masyithah; Bambang Hariyadi; Winda Dwi Kartika
BIO-SITE |Biologi dan Sains Terapan Vol. 2 No. 2 (2016): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Bukit Duabelas National Park (TNBD) has become habitat for many kinds of flora and fauna as well as the area Orang Rimba’s odyssey. Orang Rimba have interacted with the environment for many years so that produce traditional knowledge about the use of animals that are consumed. The assessment of orang Rimba’s Local wisdom is very important to document their traditional knowledge which eroded by modernization and as an effort to preserve the animals consumed. This study aimed at investigating the species or animals for consumptions, describing orang rimba’s knowledge about wildlife utilization that can be consumd, hunting and how to treat animals that consumed. The study was conducted in a Orang Rimba community in TNBD Sarolangun that is used Snowball sampling to select the respondent. The data has been obtained by descriptive analysis. Techniques of data collection through in-depth interview, observation participative, documentation, and literature. These results indicate the Orang Rimba utilized 65 species of wild animals for consumptions, it consists; 13 species of mammals, 13 species of reptiles, 1 spesies of amphibian, 11 species of brids, 21 species of pisces, and 6 species of invertebrates. Orang Rimba have knowledge in utilizing animal consumption derived from ancestors and experience. This community utilizing animal protein needs through hunting activities. They combine hunting techniques with the equipment used to obtain hunted. The ravin processed in a simple way as boiled, baked, and disalay (smoked). moreover, This study suggested further necessary documentation regarding the use of animals that are consumed by the Orang Rimba in a different location and identification of animal species consumed. Keywords: ethnozoology, animal consumptions, Orang Rimba, TNBD
Pengaruh Kadar Thiamine (Vitamin B1) terhadap Lebar Tudung Jamur Tiram Putih (Pleurotus ostreatus) dan Sumbangsihnya pada Materi Ciri dan Peran Jamur di Kelas X MA/SMA Siti Jariah; Munir Abdul Mungin; Fitratul Aini
BIO-SITE |Biologi dan Sains Terapan Vol. 2 No. 2 (2016): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Indonesia is one of the centers of biodiversity in particular areas of food, which one of them is from a species of white oyster mushroom (Pleurotus ostreatus). To improve the effectiveness and efficiency of production time associated with fulfilling the request of the oyster mushroom (Pleurotus ostreatus) is high in the market, the utilization of thiamine (vitamin B1) need to be investigated. This study aims to determine the levels of thiamine (vitamin B1), which is best used in the growth of oyster mushroom (Pleurotus ostreatus). As for the levels of thiamine (vitamin B1) used in this study use the unit ppm (parts per million), namely: B0 (control / without giving thiamine), B1 (thiamine with levels of 0.1 ppm), B2 (thiamine grading 0.2 ppm), B3 (thiamine with levels of 0.3 ppm). Parameters measured were the growth of oyster mushroom (Pleurotus ostreatus) is wide hood (cm). This study used quantitative experiments and research design complete randomized design with four treatments and six times as many repetitions as well as research along with the observations made on 21 June to 11 July 2015 (in 20 days) in the House of White Oyster Mushroom Production Nurseries, Ogan Komering Ulu Timur (OKUT). Administration of thiamine (vitamin B1) performed 2 days and watering is done every day, morning and evening using a sprayer. Low temperatures and high humidity is maintained by using hygrothermometer. Conclusions from the study and based on data analysis (ANOVA / Analysis of Variance) and F test showed that administration of thiamine (vitamin B1) does not provide an effective influence on the growth of oyster mushroom (Pleurotus ostreatus). Information thiamine (vitamin B1), which serves for the growth of oyster mushroom (Pleurotus ostreatus) is expected to be beneficial to its development as a commercial commodity mushrooms.
Pengaruh Pemberian Hormon Naftalen Acetyl Acyd (NAA) dan Kinetin pada Kultur Jaringan Nanas Bogor (Ananas comosus (L.) Merr.) cv. Queen Imam Mahadi
BIO-SITE |Biologi dan Sains Terapan Vol. 2 No. 2 (2016): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Pineapple (Ananas comosus (L.) Merr.) is one tropical fruits are highly favored by the Indonesian people and the potential for export commodities. This research is aimed to determine the effect of NAA and kinetin with different concentrations of Murashige Skoog medium (MS) to mikropropagasi Bogor pineapple (Ananas comosus (L.) Merr.) cv. Queen. The research design used was completely randomized design (CRD) factorial with two factors, each factor consists of 4 degree of concentration are: NAA hormone concentrations (0-1 ppm) and kinetin (0-3 ppm) with 3 replications. Parameters observed that the percentage of explants growing, lengt of shoot ,number of shoots and number of roots. The data were analyzed further test ANAVA and DMRT at 5% level. The results showed that the percentage of explants all treatments grew 100%, lengt of shoot is 7,73 cm in control, the highest number of shoots in treatment N0,25K3 is 13.67, and the highest number of roots in the treatment N1K0 is 11.67 weeks after initiation. The results concluded that the combined treatment N0,25K3 is the best treatment for micropropagation of pineapple cv. bogor Queen.
Sitotoksisitas Fraksi Piper porphyrophyllum terhadap Sel Kanker T47D Yus Hargono Cahyaning Yudi; Hartanto Nugroho
BIO-SITE |Biologi dan Sains Terapan Vol. 2 No. 2 (2016): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Genus Piper has been using traditionally as medicines. Piper aborescen, P. crocatum, P. Imperiale, P. longum, and P. methysticum have ability to inhibit breast cancer proliferation. This research goal is to know cytotoxic activity of Piper porphyrophyllum fraction against T47D cancer cell. Extraction it’s leaves use soxhlet with chloroform as solvent then use vacuum liquid chromatography. Cytotoxic activity of Piper porphyrophyllum fraction determined by MTT assay. The result showed that fraction group A has lowest IC50 (36.22 µg/ml).
Skrining Triterpenoid dan Formulasi Granul dari Ekstrak Daun Mengkudu (Morinda Citrifolia L.) sebagai Neuroprotektor pada Perokok Dhigna Luthfiyani Citra Pradana
BIO-SITE |Biologi dan Sains Terapan Vol. 2 No. 2 (2016): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

The objective of this research was triterpenoids screening and made granules formulafrom Morindacitrifolia leaf extract as neuroprotector for smoker. Morinda citrifolia leaf extract was made by maceration using 70% ethanol. The granules of Morinda citrifolia leaf extract was composed by 3 formulas containing 10% leaf extract of Morinda citrifolia and with different levels of manitol (20% F I, F II 25%, 30%). All of formulas comply the qualification of affects fixed corner and granules flow time that base on Farmakope Indonesia. The variation of manitol concentration significantly affects fixed corner and granules flow time. The greater level of mannitol concentration 20% to 30%produced fixed corner and granules flow time of the granulesare greater.
Potensi Isolat Bakteri Pelarut Fosfat Asal Tanah Gambut Riau dalam Memproduksi Hormon Indole Acetic Acid (IAA) dan Pengaruhnya Terhadap Perkecambahan Benih Cabai Merah (Capsicum annuum L.) Dwi Wahyuni; Tetty Marta Linda; Wahyu Lestari
BIO-SITE |Biologi dan Sains Terapan Vol. 2 No. 2 (2016): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Indole Acetic Acid (IAA) is a group of auxin hormone role in regulating the growth and development. Bacterial isolated from peat soils of Riau known activity in dissolving the phosphate in the Pikovskaya medium and Red-Yellow Podzolic Soil. The purpose of this study was to test isolates from peat soils of Riau in producing IAA hormone and its effects on the germination seeds of red pepper. The results showed that the production ofIAA in Nutrient Broth (NB) not signifficant to all treatments, but tends to be highest on GGO6 that is equal to 9.72 ± 5.91 ppm. The addition of L-tryptophan in the media NB indicated that GGO2 (24.51 ± 5.53 ppm) and GGO6 (19.61 ± 1.80 ppm) significantly to GGO4 (11.33 ± 4.12 ppm). Soaking seeds of red pepper on each bacteria tend to increase the rate of seed germination. Shoot length on GGO5 not different from GGO1, GGO2 and GGO3, but significantly different with the GGO4, GGO6 and control. While GGO1 and GGO3 also significantly different with control. Root length for all treatments significantly different with the control except GGO4.

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