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POTENSI Jatropha curcas L. SEBAGAI ANTISEPTIK PADA PERTUMBUHAN Staphylococcus aureus, Escherichia coli, dan Candida sp. Erna Harfiani; Aulia Chaerani
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Skin disease is still a health problem in the world, especially tropical countries, including Indonesia, it needs normal control of flora among others with antiseptic. Jatropha curcas L., which contains secondary metabolite compounds suspected as an antiseptic on the skin. The study aimed to evaluate Jatropha sap can inhibit the growth of Staphylococcus aureus, Escherichia coli, and Candida albicans, and to know the optimal concentration of Jatropha curcas by invitro. The antiseptic activity of Jatropha sap was tested using agar diffusion method (Kirby-Bauer) with Mueller Hinton Agar medium and Saboround Dextrose Agar at concentrations 20%, 40%, 60%, 80% and 100. The results showed that Jatropha sap can inhibit the growth of Staphylococcus aureus, and Escherichia coli but not in Candida albicans. Treatment of 100% Jatropha sap concentration is the most optimal concentration in inhibiting the growth of Staphylococcus aureus, and Escherichia coli with an inhibitory zone of 9.75 ± 0.27 mm (medium antiseptic power) and 10.61 ± 0.2 mm (strong antiseptic power). The results show that the sap of Jatropha curcas L. grown in Indonesia has a good potential to be used as an antiseptic made from nature. Penyakit pada kulit masih merupakan masalah kesehatan di dunia terutama negara beriklim tropis, termasuk Indonesia, sehingga diperlukan pengendalian flora normal diantaranya dengan antiseptik. Getah jarak pagar (Jatropha curcas L.) yang mengandung senyawa metabolit sekunder diduga berfungsi sebagai antiseptik pada kulit. Penelitian bertujuan mengevaluasi apakah getah jarak pagar dapat menghambat pertumbuhan Staphylococcus aureus, Escherichia coli dan Candida albicans, dan mengetahui konsentrasi optimal getah jarak pagar secara invitro. Aktivitas antiseptik getah jarak pagar diuji dengan menggunakan metode difusi agar (Kirby-Bauer) dengan medium Mueller Hinton Agar (MHA) dan Saboround Dextrose Agar (SDA) pada konsentrasi 20%, 40%, 60%, 80% dan 100. Hasil penelitian menunjukkan getah jarak pagar dapat menghambat pertumbuhan bakteri Staphylococcus aureus, dan Escherichia coli namun tidak pada jamur Candida albicans. Perlakuan getah jarak pagar konsentrasi 100% merupakan konsentrasi paling optimal dalam menghambat pertumbuhan Staphylococcus aureus, dan Escherichia coli dengan zona hambat 9,75 ± 0,27 mm (daya antiseptik sedang) dan 10,61 0,2 mm (daya antiseptik kuat). Hasil menunjukkan getah jarak pagar yang banyak tumbuh di Indonesia mempunyai potensi yang cukup baik untuk dipergunakan sebagai antiseptik berbahan alam. Keyword: Antiseptik, Escherichia coli, Candida albicans, Jarak pagar (Jatropha curcas L.), Staphylococcus aureus

Kemampuan Air Kelapa Muda Sebagai Antimikroba Terhadap Bakteri Escherichia coli Penyebab Diare Arif Mulyanto; Ikhsan Mujahid; Tyas Ulfah Khasanah
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Diarrhea is a circumstance when the body loses much fluid and electrolyte through feces. According to the data obtained by Ministry of Health of the Republic of Indonesia (2016), in 2015 there were 18 cases of healthemergency of diarrhea, spread over 11 provinces and 18 regencies/cities. There were 1.213 people who got the disease, 30 of them died. Partially, villagers treated diarrhea by drinking young coconut water. This research aimsto discover the effectiveness of several kinds of young coconut water as antimicrobials to bacteria that can causediarrhea (bacteria test) such as Escherichia coli. In this research the inhibitionof young coconut water sample toward above-mentioned bacteria was held. Besides, a phytochemical screeningtest was also conducted to find out the antimicrobial substances contained in young coconut water. The data of young coconut water antimicrobial testing toward Escherichia coli, wereanalyzed by employing analysis of varian (ANOVA) with F test on 95% confidence level. Data testing wasfollowed by Duncan test on 95% confidence level. Research results indicated that young coconut water had antimicrobial ability toward Escherichia coli. Based on the result ofphytochemical screening of the young coconut water (all treatments) it was only secondary metabolite tannins detected. All treatments (of the young coconut water) could inhibit tested bacteria. It is allegedly because thewater contained tannins. The water of green coconut (used as a traditional medicine) was an effective inhibitor of bacteria growth bacteri. On E. coli the inhibition zone diameter was 11,6 mm. Keywords: young coconut water, antimicrobial, bacteria, diarrhea

ISOLASI DAN IDENTIFIKASI Shigella sp. PENYEBAB DIARE PADA BALITA Fitratul Aini
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Diarrhea is the highest cause of death for toddlers. This study aims to isolate and identify the bacteria Shigella sp. causes of diarrhea in toddlers. Diarrhea samples were obtained from RSIA Annisa, Jambi City then isolation was carried out at the Biotechnology and Engineering Laboratory of the Faculty of Science and Technology, Jambi University using SSA media and dilution and biochemical tests were carried out. The results of bacterial isolates were characterized and obtained by Salmonella sp. isolates. With the characteristic of black colonies, capable produce H2S gas and can ferment sugar.

Keanekaragaman Kumbang Sungut Panjang (Cerambycidae) di Kawasan Hutan Pendidikan Universitas Jambi Tia Wulandari; Winda Dwi Kartika
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Abstrak Serangga adalah kelompok hewan yang memiliki keragaman lebih tinggi daripada yang lain. Serangga memiliki manfaat dalam ekosistem dan juga dapat membantu aktivitas manusia. Cerambycid adalah sejenis serangga yang dapat mengubah komponen organik pada kayu dan batang mati. Tujuan dari penelitian ini adalah untuk mengetahui keragaman Cerambycid di hutan pendidikan Universitas Jambi. Penelitian ini diadakan pada bulan Agustus sampai November 2016. Sampel dikumpulkan dengan menggunakan perangkap Arthocarpus di 3 tempat yang berbeda. Jumlah keramik yang telah dikumpulkan adalah 561 individu dan termasuk 14 spesies. Hasil analisis data menunjukkan tingkat keragaman 0,631. Nilai indeks keanekaragaman dapat menunjukkan kondisi hutan pendidikan Universitas Jambi yang baik sebagai habitat kumbang Cerambycid. Kata Kunci: kumbang, Cerambycid, keragaman, kumbang sungut panjang Abstract Insects are group of animals that have higher diversity than anothers. Insects have benefits in ecosystem and also can help human activities. Cerambycid is a kind of insects that can transform organic matters on wood and dead stem. The aim of this study is to know the diversity of Cerambcyid in Jambi University educational forest. It is held on August untill November 2016. Samples was collected by using Arthocarpus trap in 3 different spot. The number of Cerambycid that had collected are 561 individuals and belong to 14 species. The result of data analysis showed that Cerambcyid diversity rate is classified into medium rate with diversity value reaches 0,631. Diversity index value can show Jambi University educational forest condition which is good as the Cerambcyid beetle. Keyword: Beetle, Cerambycid, Diversity, Long Horn Beetle

KOLONISASI BAKTERI ENDOFIT PADA AKAR TUMBUHAN ANDALEH (Morus macroura Miq.) Sisri Yandila; Dwi Hilda Putri; Mades Fifendy
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 2 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Endophytic bacteria are bacteria living in plant tissues and symbiotic mutualism with their host. The excess production of bioactive substances from endophytic microorganisms among others, microorganisms are easy to grow, have shorter life cycles than plants and can produce large amounts of bioactive compounds. Endophytic bacteria found in plants generally originate from the roots and then spread through xylem tissue. Endophytic bacteria that live within the plant tissue for a certain period and are able to live by forming colonies in plant tissue without harming the host. Endophytic bacteria can be isolated from sterile plant tissue surfaces or extracted from inner plant tissue. The purpose of this study was to determine the type of bacterial isolate of edofit found in the root of Andaleh plant. The method used to isolate endophytic bacteria from the root plant Andaleh is streak plate method. Identification of bacteria macroscopically and microscopically. The results obtained were 16 isolates of endophytic bacteria from the roots of Andaleh plant seen from different colony forms. Identify microscopically with Gram staining

SELEKTIVITAS ANTIKANKER EKSTRAK DAUN SIRSAK (Annona muricata) PADA LINI SEL KANKER PAYUDARA Dina Fatmawati; Suparmi Suparmi; Iwang Yusuf; Israhnanto Isradji
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 2 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Studies have been conducted on cytotoxicity of sirsak (Annona muricata) against T47D cell line. However, the selectivity of Annona muricata leaf extract against MCF-7 breast cancer has not established. This study aimed to determine the index selectivity of Annona muricata leaf extract against MCF-7 breast cancer cell line. In this quasy experimental study using 2 kind of cell line, The MCF-7 breast cancer cell line and Vero cell line as normal cell were treated with Annona muricata leaf extract at dose 1000, 500, 250, 125, 62.5, 31.5 µg/mL respectively. The MTT assay were used to evaluate the viability of both cell line after treatment with Annona muricata leaf extract. The data of viability cells were analyzed with Probit regression to determine the IC50 value of Annona muricata leaf extract in both cell lines. The selectivity of two treatment was determine based on the selectivity index (SI) that represents the ratio of IC50 value of Annona muricata leaf extract. The IC50 value of MCF-7 and normal cell were 44.94 and 184.04 respectively with selectivity index 4. This result suggested that Annona muricata had cytotoxic effect in MCF-7 breast cancer cell line but not in normal cells. In conclusion Annona muricata leaf extract has cytotoxic effect to MCF7 breast cancer cell selectively.

EKSTRAKSI KOLAGEN IKAN MANYUNG DAN IKAN KAKAP SEBAGAI ALTERNATIF KOLAGEN Anggari Linda Destiana; Stefani Harum Sari
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 2 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Total fish consumption during 2011-2015 showed an increase of 6.27%. The waste is estimated to be about 75% of the total weight of the fish. Thirty percent of the total 75% of fish processing waste is bone and skin. The collagen sources on the market is limited to cowhide and pig skin derived. Therefore, collagen from fish is expected to be an acceptable alternative source for all people because of its high availability and no risk of disease transmission. This study aimed to determine the difference of collagen content in manyung fish and snapper fish. Collagen extraction of manyung fish and snapper fish was done using alkaline and acidic solution. The soluble collagen concentration was measured by the standard Bradford solution using a UV-Vis spectrophotometer. The collagen concentration of manyung skin, snapper skin, manyung bone, snapper bone, and snapper scales obtained were 206.167; 202.833; 106.167; 77.833; and 24.50 ppm. The skin of manyung fish has the highest collagen content compared to other samples.

KEMAMPUAN ISOLAT TUNGGAL DAN KONSORSIUM AKTINOMISETES LOKAL RIAU DALAM MENDEGRADASI HIDROKARBON MINYAK BUMI Novalita Dwi Fanny; Tetty Marta Linda; Atria Martina
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 2 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Pollution of crude oil on environmental can have an effect of nutrient uptake on plants and balance of ecosystems. Biodegradation. is one way to reduce petroleum pollution . The purpose of this research are determine the potential growth of actinomycetes local Riau (L11 and L121) in crude oil hydrocarbons at concentrations of 0%, 2%, 5% and to know their ability to degrade crude oil hydrocarbons at concentrations of 5%. This research observed the growth of each actinomycetes local Riau and the percentage of crude oil degradation by a single isolate and a consortium with gravimetric method. The results showed that the growth of L11 and L121 total population was not significantly different at addition crude oil of 2% and 5% but significantly different wth 0% (without crude oil). Test degradation of crude oil using single isolate gave significantly different results for the consortium isolates. Percentage of degradation crude oil by L11 was 23.5%, L121 was 19.2% and consortium isolate (L11 + L121) was 31.9% with an incubation time of 10 days in a minimum medium salts. This actinomycetes local Riau can be developed as biodegradation agents for crude oil on soil and water.

PERTUMBUHAN STEK Aquilaria malaccensis Lamk. DENGAN PEMBERIAN BIOURINE SAPI Liyona Noviolla; Suparjo Suparjo
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 2 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

ABSTRACT Aquilaria malaccensis is one of the agarwood-producing plants that have high economic value and has been included as a type of Appendix II by CITES, where its consequences in the formal trade,agarwood have to produced from agricultural trees instead of trees that come from nature. A.malaccensis cultivation can be done with vegetative propagation one of them by cutting. In plant propagation with cuttings required additional nutrients and natural auxin, in order to encourage roots and accelerate plant growth. One of them is by utilizing cow waste water and processed into biourine.The aim of this research was to find out the growth of A. malaccensis cuttings along with giving the biourine of cow and also the most optimal biourine concentration. The study used a completely randomized design (CRD) with 5 exercises and 3 replications. The treatment consisted of: B0 = 0% concentration biourine (without giving biourine), B1 = Biourine with a concentration of 25%, B2 = 50% biourine concentration, B3 = Biourine with a concentration of 75%, and B4 = Biourine with a concentration of 100%. The results of the study showed that biourine beef growth on A.malaccensis cuttings was seen in the parameters of plant height, leaf length, leaf width, shoot length and root length. However, it was not adequate for the number of leaves and the number of shoots. The best result for growth of A.malaccensis cuttings at75% concentrations.Keyword: Aquilaria malaccensis, RAL, Biourine

ANALISIS ANALISIS VEGETASI GULMA PADA LAHAN GAMBUT PERKEBUNAN KELAPA SAWIT (Elaeis queneensis Jacq) DI KELURAHAN TEBING TINGGI OKURA, KECAMATAN RUMBAI PESISIR, PROVINSI RIAU Ollyvia Rahmadani Pertiwi; Nofripa Herlina; Elsie Elsie
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 2 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

This study aims to determine the types of weed vegetation in oil palm plantations (Elaeis guineensis Jacq) in Tebing Tinggi Okura, Rumbai Pesisir Sub-District, Riau. The research procedure starts from the field survey, the determination of the station is done by purposive random sampling method and is determined by three research stations namely Station I (3-5 years), Station II (5-7 years), Station III (> 7 years). Data is analyzed base on density, frequency, important value index, species diversity index (H '), dominance index, evenness index, uniformity index or similarity. The results were obtained nine types of weeds that are Asystasia gangetica weeds, Calopogonium mucunoides Desv., Davallia denticulata, Dianella ensifolia L., Elaphoglossum blumeanum, Eupatorium inulifolium Kunt, Imperata cylindrica Raeusch, Nephrolepis bisserata Schott., and Wrightia javanica.

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Jambi

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