cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kab. sleman,
Daerah istimewa yogyakarta
INDONESIA
Journal of Tropical Biodiversity and Biotechnology
Published by Universitas Gadjah Mada
ISSN : 25409573     EISSN : 25409581     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Journal of Tropical Biodiversity and Biotechnology (JTBB) is an authoritative source of information concerned with the advancement of tropical biology studies in the Southeast Asia Region. It publishes original scientific work related to a diverse range of fields in tropical biodiversity, functional biology, and biotechnology.
Arjuna Subject : -
Articles 23 Documents
 1   2   3 
Search results for , issue "Vol 9, No 1 (2024): March" : 23 Documents clear
Astaxanthin Production from Green Microalga Haematococcus pluvialis under Various Bean Sprout Media Concentrations and Duration of UV Radiations Hary, Biaggi Rakhmat Rheinan; Sidharta, Boy Rahardjo; Arsiningtyas, Ines Septi
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.73763

Abstract

Astaxanthin (AX) is known as a very strong antioxidant and has been utilised in many kinds of products such as foods, pharmaceutical, cosmetics, aquaculture, etc. One of the natural resources of AX is Haematococcus pluvialis which has been investigated by some researchers in order to enhance the AX production. However, the production of AX from the microalgae is still costly, hence, this present research is proposing low-cost methods namely bean sprout media (BSM) as an alternative growth media and UV radiation. The variations of BSM concentrations (2, 4, and 6 %) and times of UV radiation (1.5 and 3 hrs) were treated to H. pluvialis in laboratory conditions. BSM 4 % treatment showed an optimum growth of the microalga at 427 x 104 cell/ml (day 8) which also exhibited macrozooid, palmella, and aplanosore phases. UV radiation for 3 hr revealed that the concentration of AX production was as much as 17.37±0.04 mg/l. The research results were potential to be developed further in order to discover better and cheaper methods for scaling up AX production. 
Plant Growth Promoting Endophytic Microorganisms from Orchids for A Sustainable Agriculture Goh, Lucky Poh Wah; Jaisi, Benardette Lyovine; Jawan, Roslina; Gansau, Jualang Azlan
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.74403

Abstract

Conventional agriculture practice has heavily relied on chemical fertilizers to increase crop yield. However, long-term application of chemical fertilizers carries tremendous negative impact on the environment and is unsustainable. Hence, the search for an alternative source of fertilizers is required. Orchids are flowers and can be found in tropical countries. The growth and development of orchids are closely tied to the presence of plant growth promoting endophytic microorganisms (PGPM). PGPM harbours various beneficial traits such as potassium and phosphorus solubilization and indole acetic acid and siderophore production which enhance and support plant growth and development. This review article showed that PGPM isolated from orchids could be utilized in conventional agriculture to reduce dependency on chemical fertilizer. 
Scanning Electron Microscopy Analysis of Tea’s Embryo Axis Explant Cultured on Murashige and Skoog Medium Containing 2,4-Dichlorophenoxyacetic acid Eskundari, Ratna Dewi; Taryono, Taryono; Indradewa, Didik; Purwestri, Yekti Asih
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.76451

Abstract

Camellia sinensis L. is an important crop in Indonesia as healthy beverage that contains several secondary metabolism compounds, such as polyphenols and catechins. Tissue culture including somatic embryogenesis and organogenesis has been used for propagating plant for various needs. In this present short-communication, scanning electron microscopic (SEM) analysis of tea was conducted and discussed. This study aimed to investigate surface ultrastructure of TRI2025 embryo axis tea clone cultured on Murashige and Skoog (MS) medium containing 2,4-Dichlorophenoxyacetic acid (2,4-D). The results revealed two different forms of explant’s development, i.e. somatic embryo and transitional form between somatic embryogenesis and organogenesis; or called by “Globular-like Structure” (GLS). Surface ultrastructure analysis of somatic embryo and GLS revealed respectively many stages of somatic embryo development i.e. globular, torpedo, and cotyledon stage, and leaf development form GLS regeneration. 
Spatial Modelling Habitat Suitability of Javan Langur (Trachypithecus auratus É. Geoffroy Saint-Hilaire, 1812) in Bromo Tengger Semeru National Park (TNBTS), East Java Ningtyas, Ari Nadya; Aryanti, Nirmala Ayu; Susilo, Tander Scila Serata Dwi; Rahmadana, Mahmuddin; Agustin, Ika Yuni
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.76841

Abstract

Javan Langur (T. auratus) is well-known as one of endemic primates from Java, Bali and Lombok Islands. The activities of land clearing, vegetation conversing, wild hunting and illegal wildlife trading are the main causes of the extinction of the Javan Langur. It can be used as an important issue for conservation action by making prediction maps of suitable habitat potential, especially for species facing a high risk of extinction in the wild.  We were documenting an information about potential habitat for Javan langur using spatial suitability model in order to provide rigorous information as the basis for conservation activities of Javan langur in TNBTS. We used Landsat-8 TM image and geospatial data to support analysis as a representative of environmental parameters in order to develop the habitat model. We were using maximum entropy (MaxEnt) algorithm refers to Javan langur presence or absence. The results showed that the suitability of the Javan langur habitat in TNBTS has an excellent model accuracy level with an AUC (Area Under the Receiver Operating Characteristics) value of 0.964 and a standard deviation of 0.961. Parameters with the highest response values here are elevation, NDVI (Normalised Difference Vegetation Index) and temperatures. 
The Growth Response of Rendeu (Staurogyne elongata (Neese) Kuntze) to Shoot Pruning and Its Propagation by Shoot Cutting Lailaty, Intani Quarta; Astutik, Sri; Surya, Muhammad Imam
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.77078

Abstract

Rendeu (Staurogyne elongata (Neese) Kuntze) is a native Indonesian plant used as food and traditional medicine in the daily life of the people residing around Gunung Halimun-Salak National Park. Due to the potential source of herbal-based medicines and traditional food in the long-run purposes, the proper method of its propagation is required, so that Rendeu can be conserved and utilised sustainably. This study employed two research designs. First, a completely randomized design with pruning and IAA (indole-3 acetic acid) treatment was used for seedling growths. Second, plant propagation applied a factorial randomized block design: planting media types and plant growth regulator (PGR) (rootone F) treatment. Observation included the number of buds, number of leaves, number of flowers, plant biomass, root length, and relative chlorophyll content using the SPAD tool. The data were analysed using ANOVA (SPSS ver. 17.0), followed by Pearson correlation analysis. The results showed that applying IAA and leaf pruning could increase the number of buds, the number of leaves and the fresh weight of S. elongata plants compared to the control plant. The addition of rootone increased the growth of Rendeu shoot cuttings, shown in all growth parameters and chlorophyll content. Humus was the best media for Rendeu’s growth among all planting medium.  Planting media affected the increase in the number of leaves and the number of buds of S. elongata significantly. The interaction of planting media and PGR somewhat influenced root length and total leaf chlorophyll. The growth and production of S. elongata increased with the time of planting. 
Identifying Single Nucleotide Polymorphisms (SNPs) in OsFER1 and OsFER2 Genes Linked to Iron accumulation in Pigmented Indonesian Rice (Oryza sativa L.) Pratiwi, Apriliana; Bassalamah, Rizka Fahma; Elvani, I Sabila; Sebastian, Alfino; Purwestri, Yekti Asih
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.78019

Abstract

Iron (Fe) is an essential micronutrient for the well-being of plants, animals, and bacteria. In plants, iron plays a pivotal role in a myriad of metabolic processes, encompassing redox reaction, photosynthesis, respiration, chlorophyll synthesis, and nitrogen fixation. For humans, iron is indespensable for several metabolic functions, particularly in the synthesis of haemoglobin. Iron deficiency can lead to health issues on a global scale, therefore identifying key crops, such as rice for providing sufficient iron in diet intake is very important. In rice, the maintenance of iron homeostasis is orchestrated by various genes, with OsFER1 and OsFER2 acting as iron accumulator genes in leaves, stems, flowers, and grains. The primary objective of this study was to ascertain the single nucleotide polymorphisms (SNP) in the OsFER1 and OsFER2 and to assess the iron content in Indonesian local rice cultivars. To achieve this, we examined partial sequences of OsFER1 and OsFER2 to identify SNPs in the Indonesian rice cultivars used (Cempo Ireng, Pari Ireng, Hitam Kalsel, Merah Pari Eja, and Ciherang). Concurrently, the iron content in the seeds was quantified using Atomic Absorption Spectrophotometry (AAS). The analysis revealed that the OsFER1 gene sequence, specifically exon 5, exhibited a SNP in the form of a transition. In contrast, the OsFER2 gene sequences, specifically in intron 2 displayed SNPs in the form of insertions. Notably, the iron content in the seeds was highest in Cempo Ireng (black rice), while it was lowest in Merah Pari Eja (red rice) and Ciherang (non-pigmented rice). Importantly, the identified SNPs in these partial gene sequences did not exert any discernible influence on iron levels or the formation of ferritin protein. 
Growth Kinetic Modelling of Efficient Anabaena sp. Bioflocculation Rahmawati, Amalia; Rohmawati, Irma; Nurafifah, Istini; Sadewo, Brilian Ryan; Suyono, Eko Agus
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.82196

Abstract

Bioflocculation is a harvesting technique that employs flocculant agents such as bacteria and microalgae. The benefit is the absence of a chemical-added flocculant. Because bacteria need a particular medium, microalgae flocculant agents are more effective. This study used Anabaena sp. to collect fat, protein, and carbohydrates from the Glagah consortium. Three replications of those microalgae were grown in 300 ml of Bold Basal Medium culture for eight days. On the day of harvest, flocculant microalgae (Anabaena sp.) and non-flocculant microalgae (Glagah) were combined to accomplish flocculation. On the day of harvest, parameters were observed by combining Anabaena sp. with the Glagah consortium in the ratios 1: 1, 0.5: 1, and 0.25: 1. There were three times of each parameter test. Utilizing a wavelength of 750 nm, the proportion of precipitation was calculated spectrophotometrically. Bligh and Dyer were used to measure the lipids. The phenol sulfate technique was used to calculate the amount of carbohydrates. By employing the Bradford method, proteins were quantified. Biofocculation percentages and carbohydrate content were optimum on a ratio of 0.25:1. Lipid and protein content were optimum on a ratio of 1:1.
A Mini Review on Analysis of Potential Antibacterial Activity of Symbiotic Bacteria from Indonesian Freshwater Sponge: An Unexplored and A Hidden Potency Setiawan, Edwin; Hermanto, Michael Einstein; Abdulgani, Nurlita; Prasetyo, Endry Nugroho; Riani, Catur; Wulandari, Dyah; Budiharjo, Anto
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.82682

Abstract

Marine sponges have been investigated as potential bioresources because of their symbiotic relationship with microbes such as Actinobacteria that produce antibacterial substances. In contrast, a group of sponges, that inhabits freshwater environments called freshwater sponges (Order Spongillida Manconi & Pronzato, 2002) and consists of only one percent among all of the sponges’ species (Phylum Porifera Grant, 1836), has  not yet intensively examined.  For this reason, we screened, determined, evaluated, and reviewed by examining several databases in Scopus, Pub Med, and Google Scholar related to potential aspects of symbiotic bacteria and their antibacterial substances that can be further utilised  and developed into synthesised  antibacterial compounds, based on published metagenomic data of symbiotic bacteria in freshwater sponges. At the same time, we compared a composition of those freshwater symbionts to marine sponges’ symbionts whether those possess a similar composition or not. Moreover, a current report and a revisit study of freshwater sponges in East Java, initiate further direction on mapping of those symbiotic bacteria from Indonesia that can be nominated as potential groups possessing antibacterial properties. 
Chrysin Inhibits Indonesian Serotype Foot-and-Mouth-Disease Virus Replication: Insights from DFT, Molecular Docking and Dynamics Analyses Susilo, Agus; Cahyati, Miftakhul; Nurjannah, Nurjannah; Pranowo, Dodyk; Hermanto, Feri Eko; Primandasari, Elma Putri
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.83140

Abstract

Chrysin, a predominant compound in Propolis, possesses diverse bioactivities, including antiviral properties. However, its antiviral efficacy against the Indonesian Foot-and-Mouth Disease Virus (FMDV) serotype remains unexplored. This study investigates Chrysin's inhibitory potential against FMDV Indonesian serotype by targeting the 3C Protease (3CP), a vital enzyme for viral replication. Multiple sequence alignment was used to reveal unique characteristics of the Indonesian serotype's 3CP compared to global serotypes. Density Functional Theory (DFT) calculations assessed Chrysin's interaction with 3CP based on electronegativity. Molecular docking and molecular dynamics analyses evaluated Chrysin's inhibitory activity against 3CP, using homology modeling for the Indonesian serotype's 3CP structure. Luteolin, a known FMDV 3CP inhibitor with a similar structure to Chrysin, served as a reference. Results showed distinct 3CP sequences in the Indonesian serotype compared to O serotypes and others. Chrysin exhibited potential electron-donor activity with lower HOMO and LUMO values than Luteolin, but they had similar energy gaps, i.e., 4.016 and 4.044 eV, respectively. Molecular docking indicated similar binding affinities, with Chrysin (-6.365 kcal/mol) and Luteolin (-6.864 kcal/mol) bound to active site residues. Molecular dynamics analysis demonstrated stable 3CP-Chrysin and 3CP-Luteolin complexes, with minor differences in Radius of gyration (Rg) and Root-Mean-Square Fluctuation (RMSF) below 1 Å. From the ligand stability point of view, Chrysin had comparable stability with Luteolin. However, Chrysin formed fewer hydrogen bonds and displayed greater free-binding energy than Luteolin during simulation periods. These findings suggest that Chrysin holds promise as an inhibitor of the Indonesian serotype's FMDV 3C Protease. 
Diversity of Santigi (Pemphis acidula J.R.Forst. & G.Forst.), A Mangrove Association in Tomini Bay, Sulawesi, Indonesia Baderan, Dewi Wahyuni K.; Rahim, Sukirman; Angio, Melisnawati H.; Akbar, Muh. Nur; Jannah, Magfirahtul; Retnowati, Yuliana; Utina, Ramli
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.83889

Abstract

Pemphis acidula is a wild plant in rocky or sandy coastal areas and mangrove ecosystems. Different geographic characteristics may affect plant adaptability and have an impact on the emergence of various genotypes. This study was performed to reveal the phenetic relationship and genetic variation of P. acidula in 3 different areas in Tomini Bay, Gorontalo Province, Indonesia. We took 3 samples from each location and analysed them using 14 morphological characters and molecular approaches based on ISSR markers and ITS gene. The results showed that P. acidula on Olele had bigger sizes in some morphological features compared to the plants in other study areas. The phenetic analysis showed that P. acidula at Biluhu and Dulanga were more closely related, although P. acidula at the 3 locations had 100% similarity. Genetic variation analysis showed the highest genetic similarity based on ISSR markers was found in Dulanga and Biluhu samples (76.8%). Phylogenetic based on ITS gene revealed that Olele samples were in the same clade with P. acidula accession from GenBank (genetic distance 0-0.19%), while Biluhu samples were a sister group (genetic distance 24.97-25.03%) even though their percentage identity corresponds to P. acidula (81.34%). Plant adaptation to different habitat conditions may affect the genetic diversity of P. acidula. 

Page 1 of 3 | Total Record : 23

 1   2   3 

Filter by Year

2024 2024


Reset
Filter By Issues
All Issue Vol 9, No 4 (2024): December Vol 9, No 3 (2024): September Vol 9, No 2 (2024): June Vol 9, No 1 (2024): March Vol 8, No 3 (2023): December Vol 8, No 2 (2023): August Vol 8, No 1 (2023): April Vol 7, No 3 (2022): December Vol 7, No 2 (2022): August Vol 7, No 1 (2022): April Vol 6, No 3 (2021): December Vol 6, No 2 (2021): August Vol 6, No 1 (2021): April Vol 5, No 3 (2020): December Vol 5, No 2 (2020): August Vol 5, No 1 (2020): April Vol 4, No 3 (2019): Desember Vol 4, No 2 (2019): August Vol 4, No 1 (2019): April Vol 3, No 3 (2018): December Vol 3, No 2 (2018): August Vol 3, No 1 (2018): April Vol 2, No 2 (2017): December Vol 2, No 1 (2017): June Vol 1, No 2 (2016): December Vol 1, No 1 (2016): June More Issue