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All Journal Vegetalika Jurnal Produksi Tanaman Journal of Tropical Biodiversity and Biotechnology BIOMA : Jurnal Biologi Makassar Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Bio Educatio : The Journal of Science and Biology Education Jurnal Pengabdian Magister Pendidikan IPA Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Gema Ngabdi International Journal of Educational and Life Sciences (IJELS)
Ratna Dewi Eskundari
Universitas Veteran Bangun Nusantara
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Public Health Social Sciences Other

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Protein Profile of Tissue Culture of TRI2025 Tea Clone Eskundari, Ratna Dewi; Taryono, Taryono; Indradewa, Didik; Purwestri, Yekti Asih
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (448.215 KB) | DOI: 10.15294/biosaintifika.v11i1.17522

Abstract

Tea is well known as favourite healthy drink for almost all people over the world. Tea propagation using conventional and modern ways are now developing rapidly. However, information regarding the protein profile of tissue culture of tea plant has not been revealed yet. This study aimed to determine the difference of protein profile of tea’s tissue culture using SDS-PAGE. This study was conducted using embryonic axes of TRI2025 tea clone cultured on MS media supplemented with 2,4-D for inducing somatic embryogenesis and globular-like structure (GLS) regeneration, and MS media supplemented with BAP for inducing shoot via organogenesis. The results revealed that proteins in the size of 37.69; 54.89; 60.77; 71.35; 87.34; and 92.99 KDa might be involved at somatic embryogenesis, and about 38.69 KDa, 69.27 KDa, and 55.76 KDa respectively for GLS, initiation of shoot, and initiation of GLS derived leaf. Predicted key protein for leaf initiation both directly or through GLS was about 31-33 KDa, while for callusing were about 27.56 and 52.73 KDa, and constitutive protein was about 22.75 KDa. This study provides the first report of protein profile of tea’s tissue culture. The information obtained can be beneficial as a marker for explant for somatic embryogenesis, GLS, or organogenesis pathway and as a scientific information for further biotechnology development.
Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture Ratna Dewi Eskundari; Taryono Taryono; Didik Indradewa; Yekti Asih Purwestri
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 3 (2021): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.64403

Abstract

Tissue culture is an alternative choice of plant propagation either through somatic embryogenesis or in vitro organogenesis techniques. TRI2025 tea clone has been cultured successfully, however, the scientific information related to morphology, histology, and protein profile at an early event of culturing time has not been reported yet. This study aimed to determine the differences between those pathways, in the context of morphology, histology, and protein profile. The explants were the embryo axis of TRI2025 tea clone cultured on two different induction mediums; somatic embryogenesis and in vitro organogenesis induction medium. The results showed that most of the explants cultured on A medium developed to be a globular-like structure at 11-day after culture (DAC), while all explants cultured on B medium showed the initiation stage of in vitro organogenesis. Histological analysis showed meristem reconstruction at shoot apical meristem (SAM) and root apical meristem (RAM) at 11-DAC at explants cultured on B medium, while explants cultured on A medium showed callusing at 21-DAC. Protein profile analysis using SDS-PAGE showed protein bands of 54 and 81 KDa that only appeared at explants cultured on A medium start from 14-DAC, and those two protein bands thought to be a differentiator at the early stages of the two tissue culture techniques. Thus, these parameters can be used as early detection for plant tissue culture, especially in tea. 
Budidaya Jaringan Tanaman Teh di Indonesia Ratna Dewi Eskundari
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 5 No. 2 (2020)
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v5i2.9791

Abstract

Teh (Camellia sinensis L.) merupakan salah satu bahan baku untuk minuman teh. Usaha peningkatan produksi teh akhir-akhir ini gencar dilakukan mengingat meningkatnya populasi penduduk dunia. Perbanyakan tanaman teh dapat dilakukan secara konvensional menggunakan biji ataupun stek daun (stek batang). Selain dua cara tersebut, teh juga dapat diperbanyak melalui budidaya jaringan baik menggunakan jalur embriogenesis somatik ataupun organogenesis. Perbanyakan melalui cara ini mulai dikembangkan di Indonesia walaupun belum sebanyak yang dilakukan dengan stek batang. 
Pengaruh Pemberian Air Rendaman Teh terhadap Pertumbuhan Stek Singkong Rohmat Faoji; Ratna Dewi Eskundari; Nur Rokhimah Hanik
BIO EDUCATIO : (The Journal of Science and Biology Education) Vol 6, No 2 (2021): Bio Educatio (The Journal of Science and Biology Education)
Publisher : Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.345 KB) | DOI: 10.31949/be.v6i2.3298

Abstract

Singkong merupakan salah satu alternatif makanan pokok dan mudah diperbanyak menggunakan stek. Penelitian ini bertujuan untuk mengetahui pengaruh pupuk organik berupa air rendaman cangkang telur, air rendaman teh, dan air rendaman kulit bawang merah, terhadap pertumbuhan stek singkong. Hasil penelitian menunjukkan bahwa parameter panjang dan jumlah daun menunjukkan perbedaan yang signifikan di antara perlakukan yang diberikan, yaitu panjang daun tertinggi didapat dengan mengaplikasikan air rendaman cangkang telur, sedangkan parameter jumlah daun tertinggi diperoleh dengan mengaplikasikan air rendaman ampas teh. Parameter panjang akar dan panjang tunas tidak menunjukkan hasil yang signifikan, tetapi perlakuan air rendaman teh memberikan hasil terbaik dalam menginduksi panjang tunas tertinggi. Dengan didapatkannya hasil penelitian ini diharapkan dapat memberikan sumbangan ilmiah bagi petani singkong pada khususnya sehingga diharapkan dapat menambah kuantitas panen singkong per musim tanam. 
Pemilihan Bagian Eksplan pada Stek Batang Tanaman Sambung Nyawa Ratna Dewi Eskundari; Nur Rohimah Hanik
Jurnal Biologi Tropis Vol. 20 No. 2 (2020): Mei - Agustus
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.384 KB) | DOI: 10.29303/jbt.v20i2.1791

Abstract

Abstrak: Tanaman sambung nyawa (Gynura procumbens) dikenal sebagai salah satu tanaman yang bermanfaat bagi kesehatan tubuh. Daun tanaman ini diketahui mempunyai efek anti peradangan yang dapat mengurangi bahkan mengobati berbagai macam penyakit. Penelitian ini berfokus pada pemilihan bagian batang tanaman sambung nyawa yang mempunyai kapasitas terbaik sebagai eksplan stek. Penelitian ini dilakukan dengan menanam 3 bagian batang tanaman sambung nyawa yang berbeda, yaitu bagian atas (pucuk sampai dengan 10 cm ke bawah), tengah (bagian batang yang berjarak 10cm dari pucuk sampai 20 cm), dan bawah (bagian batang yang berjarak 20 cm dari pucuk hingga 30 cm). Ketiga jenis eksplan ini ditanam di tanah yang mengandung humus tanpa diberi perlakuan penambahan ZPT. Penelitian ini menggunakan rancangan acak lengkap dengan sepuluh ulangan. Hasil penelitian menunjukkan bahwa tidak ada perbedaan yang nyata antara bagian batang dengan panjang daun, tetapi terdapat perbedaan yang nyata dengan lebar daun, panjang tunas, dan panjang akar. Hasil penelitian ini diharapkan dapat menjadi rujukan bagian batang “tengah” dan atau “bawah” sebagai eksplan terbaik dalam usaha perbanyakan tanaman sambung nyawa menggunakan stek. Kata kunci: organogenesis, perbanyakan, sambung nyawa, stek.Abstract: Sambung nyawa plant (Gynura procumbens) is known as one of the plants that are beneficial for human health. The leaves of this plant are known to have anti-inflammatory effects that can reduce and even treat various diseases. This research focused on the selection of the stem part of sambung nyawa plant which has the best capacity as explant cuttings. This research was carried out by planting three different parts of the stem of the plant, namely the upper part (shoots apical up to 10 cm down part), the middle (the stem part that is 10cm from the top to 20 cm down part), and the bottom (the part of the stem which is 20 cm apart from shoots up to 30 cm down part). These three types of explants were planted in soil containing humus without being treated with the addition of PGR(s). This study used a completely randomized design with ten replications. The results showed that there was no significant differences between the stem parts with leaf length parameter, but there were significant differences in leaf width, shoot length, and root length ones. The results of this study were expected to be a reference part of the stem "middle" and or "bottom" as the best explant in the effort to propagate the sambung nyawa plants using cuttings.Keywords: organogenesis, propagation, sambung nyawa, cuttings. 
The Effect of Peanut Skin Compost Mix Variaries on Planting Media on the Growth of Dendrobium Sp Nur Rokhimah Hanik; Sri Harsono; Ratna Dewi Eskundari
Jurnal Biologi Tropis Vol. 21 No. 1 (2021): Januari - April
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i1.2465

Abstract

Ferns are a growing medium for orchids that are starting to be protected so that their availability is very limited and quite expensive, therefore a solution is needed to reduce maintenance costs by utilizing peanut shell compost as a mixture of media. Peanut shell compost can act as a medium and fertilizer. The purpose of this study is: 1. To determine the effect of various peanut shell compost mixtures in growing media on Dendrobium orchid growth. 2. To determine the best composition of peanut shell compost mixture for Dendrobium orchid growth. The results of this study serve as information material for Dendrobium orchid collectors and entrepreneurs about the effect of various peanut shell compost mixtures in growing media on the growth of Dendrobium orchids, as well as a reference material for further research related to efforts to improve Dendrobium orchid cultivation, especially the use of peanut shells as a medium. Grow as well as compost to increase the growth of Dendrobium orchids. The research period was started from July 2020 to December 2020. The research object was a 12 month old Dendrobium orchid, the research location was in Gabahan RT 05 RW 12 Jombor Bendosari Sukoharjo. Simple randomized research design with four treatments. Treatment A / K.0 of fern growing media without peanut shell compost, treatment B / K.1 / 2 of fern media mixed with compost with a ratio of 1: 1, treatment C / K.1 / 3 media fern mixed with peanut shell compost with a ratio 2/3: 1/3, and treatment C / K.1 / 4 media of fern mixed with peanut shell compost in the ratio 3/4: 1/4. Data were measured after 12 weeks from planting. Data analysis with Variance Test (Anava test) and advanced Duncan Multiple Range Test (DMRT).  From the research it can be concluded that the variation of the peanut shell compost mixture in the growing medium has a significant effect on the growth of Dendrobium orchids on four (4) observed growth parameters (Leaf Length, Number of Leaves, Leaf Area, and Wet Weight. Moderate for the Number of tillers. There was a difference in effect, although this difference was not significant. And the treatment of compost mixture B / K.1 / 2 or with a ratio of 1: 1 gave the best effect on all observed growth parameters (Leaf Length, Number of Leaves, Leaf Area, Number of Tillers, and Gross weight. There is still a need to develop further research on peanut shell compost as a mixture for growing media, because in addition to reducing costs for growing media it can also reduce costs for purchasing fertilizer, because peanut shell compost contains a lot of N, P, and K elements. soil on growing media can reduce maintenance costs.
Phytochemical test of several eco-handsanitizer candidates Ratna Dewi Eskundari; Tri Wiharti; Nur Rokhimah Hanik; Fety Fatimah; Umi Salamah; Antik Murwani
Jurnal Biologi Tropis Vol. 22 No. 1 (2022): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i1.3258

Abstract

Ecoenzymes are natural ingredients formed from the fermentation of fruit and or vegetable residues that have many benefits. One of the benefits of ecoenzymes is that they can be used as eco-handsanitizers; which can be used as a candidate for handsanitizer for those who are allergic to alcohol-based handsanitizer. This study aims to determine the pH, antibacterial, antiviral, and antifungal content of the candidate eco-handsanitizer solution through alkaloid, flavonoid, and saponin test, also fungal-inhibition test. The candidate eco-handsanitizer solution was made from an ecoenzyme solution derived from fruit or vegetable residue and added with aloe vera gel and vitamin C or E. The results showed that eco-handsanitizer candidate solution derived from ecoenzyme solution with a dilution of 1:500 or 1:4 and added with aloe vera and vitamin E had a pH that tends to be acidic after 5 weeks of manufacture. The candidate eco-handsanitizer solution contained alkaloid compounds up to 2nd week for 1:500 dilution or up to 5th week for 1:4. Fungal test also showed positive results of one of the treatments in inhibiting fungal growth until the 11th day. These results were expected to be used as guidelines for further research, such as quantitative and qualitative tests for more sensitive alkaloids, for example using GC-MS. 
The Effect of Planting Media Composition on the Growth of Dendrobium Sp. Orchid Seedlings Nur Rokhimah Hanik; Ratna Dewi Eskundari; Tri Wiharti
Jurnal Biologi Tropis Vol. 22 No. 2 (2022): April - June
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i2.3446

Abstract

Fern is the best growing medium for orchids, because it is protected, a solution is needed to find a substitute for fern media. In this study, a combination of stem charcoal, husk charcoal and peanut shell compost was chosen. The combination of the three was chosen because the charcoal sticks and husk charcoal are relatively sterile and the peanut shell compost can function as a medium and fertilizer. The purpose of this study 1. To determine the effect of the composition of the planting medium on the growth of Dendrobium sp orchid seeds 2. To determine the best composition of the growing media for the growth of Dendrobium sp orchid seeds. The research time was 2.5 months, the object of the research was the Dendrobium sp. orchid aged 12 months, the research location was at Paranet's house, Gabahan Village, RT 05 RW 12, Jombor Sukoharjo. Simple randomized study design with 4 treatments. Treatment A Planting orchid seeds with a mixture of ferns and peanut shell compost in 10 pots. Treatment B was planting orchid seeds with a mixture of stem charcoal, husk charcoal, and peanut shell compost in a ratio of 1:1:1 for 10 pots. Treatment C was planting orchid seeds with a mixture of charcoal stems, husk charcoal and peanut shell compost in a ratio of 1/4:1/4:1/2 for 10 pots. Treatment D was planting orchid seedlings. The variables observed were the number of leaves, leaf length, leaf area, number of tillers, and wet weight measured 12 weeks after treatment. Data analysis using Varieties Test (Anova or Kruskal Wallis Test) followed by Duncan's Multiple Range Test (DMRT) or Mood Median Test. Growth parameters were observed (leaf length, number of leaves, leaf area, and wet weight). As for the number of tillers, there was a difference in influence, although the difference was not significant. Treatment The combination of K.1/2 media or with a comparison of peanut shell compost, charcoal stems and husk charcoal 1/2:1/4:1/4 gave the best effect on the 4 observed growth parameters (Leaf Length, Leaf Number, Leaf Area). , and wet weight). From the results of this study, collectors, orchid lovers and orchid entrepreneurs can use a combination of stem charcoal, husk charcoal and peanut shell compost as a substitute for ferns with a mixture of charcoal stems, husk charcoal, and peanut shell compost in a ratio of 3/8:3/ 8:2/8.
Pelatihan Pembuatan Eco-enzyme sebagai Handsanitizer di RT.001/008 Kelurahan Bulakrejo Kecamatan Sukoharjo Kabupaten Sukoharjo dalam Upaya Mewujudkan Desa Mandiri Tangguh Covid-19 Ratna Dewi Eskundari; Tri Wiharti; Nur Rokhimah Hanik
Jurnal Pengabdian Magister Pendidikan IPA Vol 4 No 4 (2021)
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.638 KB) | DOI: 10.29303/jpmpi.v4i4.1256

Abstract

The Covid-19 pandemic which has lasted for about 2 years has had a negative impact in almost all parts of the world, including in this partner communities. This community service activity aims to provide training to partner communities regarding the manufacture of one of the ecoenzyme diversification products in the form of an eco-handsanitizer. This activity has a sequence of situation analysis of partner communities, counseling and training on making eco-handsanitizer, and evaluation. The evaluation results showed that the community partners can make eco-handsanitizers after this outreach.
Pemberdayaan Masyarakat selama Pandemik Covid-19 di RT 001/008 Bulakrejo Kecamatan Sukoharjo Kabupaten Sukoharjo Ratna Dewi Eskundari; Agus Purwanto; Suwarto
Jurnal Pengabdian Magister Pendidikan IPA Vol 5 No 1 (2022): Januari - Maret
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.395 KB) | DOI: 10.29303/jpmpi.v5i1.1358

Abstract

The Covid-19 pandemic has a negative impact, especially in the economic sector throughout the world, including in Indonesia, and in this activity is the RT community. 001/008 Bulakrejo Sukoharjo Regency. The purpose of this community service is to empower the community in RT 001/008, Bulakrejo Village, Sukoharjo District, Sukoharjo Regency during the Covid-19 pandemic. The method used in this service was the lecture and mentoring method. The material presented was the manufacture of ecoenzyme with raw materials in the form of fruit and vegetable waste. Initial observations of partners' conditions showed that the community in RT 001/008, Bulakrejo Village, Sukoharjo District, Sukoharjo Regency has not been able to make environmentally friendly ecoenzyme that can be economically useful, for example, can be used as plant biological fertilizer or fish feed. For this reason, further assistance was needed so that in making this ecoenzyme more acceptable to the community so that it can make more production and a wider market. The results of community service activities carried out three times showed that partner communities can practice making and harvesting ecoenzymes. Thus, it can be said that this activity succeeded in meeting its target.
Co-Authors Agus Purwanto Agus Purwanto AGUS PURWANTO Ahmad Rosyid Alya Febrina Azzahra Antik Murwani Anwari Adi Nugroho, Anwari Avivi Khoirunnida Cahyanti, Fiky Ariska Cahyanti, Fiky Ariska Cahyanti Dian Andhi Saputra Didik Indradewa Didik Indradewa Didik Indradewa Fety Fatimah Fiky Ariska Cahyanti Fitriani, Rindi Diah Ayu Fitriani Marta, Fadhillah Mudinillah, Adam Muhammad Nur Hidayad Nur Rohimah Hanik Nur Rokhimah Hanik Oktavianingtyas, Desty Oktavianingtyas Pangestu, Prestiani Yulia Qurota A'yun Rindi Diah Ayu Fitriani Riska Satya Graha Putrimulya Rohmat Faoji Sri Harsono Sri Harsono, Sri Suwaji Handaru Wardoyo Suwaji Handaru Wardoyo Suwaji Handaru Wardoyo Suwarto Suwarto Taryono Taryono Taryono Taryono Taryono, Taryono Tri . Wiharti Tri Wahyuni Tri Wiharti Tri Wiharti Tri Wiharti Umi Salamah Wahyuni, Tri Wahyuni Yekti Asih Purwestri Yekti Asih Purwestri