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INDONESIAN JOURNAL OF PHARMACY

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Published by Universitas Gadjah Mada

ISSN : 23389427 EISSN : 23389486 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education (DGHE) DIKTI No. 58/DIKTI/Kep/2013.

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Articles 706 Documents

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SYNTHESIS AND ANTIMICROBIAL ACTIVITY EVALUATION OF SOME SCHIFF BASES DERIVED FROM 2-AMINOTHIAZOLE DERIVATIVES Gupta, Rajul; Fuloria, Neeraj Kumar; Fuloria, Shivkanya
Indonesian Journal of Pharmacy Vol 24 No 1, 2013
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Various substituted acetophenones (1-5) on treatment with iodine and thiourea yielded 2-amino-4-(substituted-phenyl)thiazole (1a-5a), which on further treatment with various substituted aldehydes to get N-(substitutedbenzylidene)-4(substitutedphenyl)thiazol-2-amine (1b-5b). All the synthesized compounds were characterized by their respective FTIR, H NMR and Mass data. Synthesized compounds (1b-5b) were subjected to investigation for their antibacterial and antifungal studies against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Asperigillus flavus and Asperigillus fumigatus by disk diffusion method. Compound 5b was found to be most effective with largest zone of inhibition. Key words: Thiazole, Acetophenones Antibacterial, Antifungal, Substituted Aldehydes.

Econazole depleted calcium release-activated calcium (CRAC) current through blockade of voltage-dependent Ca 2+ channels Agung Endro Nugroho; Hari Purnomo; Kazutaka Maeyama
Indonesian Journal of Pharmacy Vol 22 No 2, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Econazole is an azole antifungal agent which can block the calcium release-activated calcium (CRAC) current in human leukaemic T cell line. The phenomenon is also possible to occur in mast cell such as RBL-2H3 (rat basophilic leukemia) cells, a tumor analog of mast cells. In the study, we investigated effect of econazole on 45Ca2+ uptake into the cells in response to thapsigargin, an ATP-dependent Ca2+ (SERCA) inhibitor, by direct measurement of radiolabelled Ca2+ uptake in cells. The mechanism underlying this effect of econazole was studied using molecular modelling. In present study, econazole inhibited 45Ca2+ influx into mast cells in absence of mast cells inducer, thapsigargin. Moreover, econazole potently suppressed the 45Ca2+ influx induced by thapsigargin. It was supported that econazole also inhibited Ca2+-induced tracheal contraction. The increase of Ca2+ was stimulated by the opening of voltage-dependent Ca2+ channels activated by KCl-induced membrane depolarization. Based on molecular docking study, score of interaction (equal to energy of interaction) of 3FGO, a main protein target on Ca2+ -ATPase, with native ligan, thapsigargin and econazole were -76.941, -117.205, and -92.277, respectively. The interaction of thapsigargin and Ca2+ -ATPase was more stable than this of econazole and Ca2+ -ATPase. It suggests that it would be difficult for econazole to block the interaction of thapsigargin with Ca2+ -ATPase to increase intracellular Ca2+.In conclusion, econazole inhibited the increase of intracellular Ca2+involving the blokade of voltage-dependent Ca2+ channels, but not involving the Ca2+ -ATPase pathway.Key words :econazole, Ca2+ -ATPase, CRAC current, thapsigargin

Antiseptic activity evaluation of piper leave from Piper betle Linn extract in hand gel antiseptic preparation Sari, Retno; Isadiartuti, Dewi
Indonesian Journal of Pharmacy Vol 17 No 4, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

It is known that piper leaves (sirih) from Piper betle Linn has been used traditionally as medicine, for example for mouthwash and wound curing. The usage of hand gels antiseptic preparation was getting increased in the current time as the incidence of desease caused by virus and bacteria like SARS appears.This research was aimed to develop preparation of hand gels from piper leaves extract obtained from extraction with hot water. The formulation of gels were made using 5%, 10%, 15%, 20% and 25% of piper leaves extract in Carbopol gel base. The evaluation of antiseptics activity was carried out using the Replica method. A series of test was done by pouring the gels at hand palm and slightly tap the thumb fingerprint to nutrient agar media. The media was incubated at 37°C for 24 hours,and the number of colonies of microorganism was counted. The antiseptic activity of the gels also compared with two hand gels antiseptic preparation containing ethanol and triclosan as antiseptic agentThe result showed that the piper leaves extract gels had a transparent pale yellow performance. The gel preparation with concentration of 15% extract had activity to reduce the microorganism of skin normal flora up to 50%. Yet the 25% concentration of piper leave extract could eliminate all the microorganism. It was also known that the 15% piper leaves extract gel had no significance different with the ethanol preparation, since the 20% and 25% piper leaves extract gels had similar antiseptic activity to the triclosan preparation.Key words: piper leaves extract, gel, antiseptic activity, replica methods

Isolation and structure identification of new alkaloids from the sponge Rhabdastrella rowi Hertiani, Triana; Edrada, RuAngelie; Van Soest, Rob W.M.; ., Sudarsono; Proksch, Peter
INDONESIAN JOURNAL OF PHARMACY Vol 19 No 3, 2008
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Chemical investigation on marine sponge Rhabdastrella rowi collected from Bali, Indonesia has been performed. This study was aimed to isolate and to identify structures of the sponge secondary metabolites as well as to test their cytotoxicity activity on mouse lymphoma cell line L5178Y.Isolation procedure was performed by using different chromatography techniques. NMR spectroscopy and mass spectrometry methods were used to identify the compounds chemical structures. Cytotoxicity of the isolates was tested on mouse lymphoma cell line L5178Y by using the microculture tetrazolium (MTT) assay.This study yielded two new alkaloids, quinolin-4-ol (1) and quninazolin-4-amine (2) which were found as minor constituents of Rhabdastrella rowi. Both compounds were observed as inactive against mouse lymphoma cell line L5178Y.Key words: Rhabdastrella rowi, sponge, alkaloids

Cost-minimization analysis of intravenous sulbenicillin versus coamoxiclav for cesarean section Tri Murti Andayani; Umi Athijah; Ali Ghufron Mukti
Indonesian Journal of Pharmacy Vol 15 No 4, 2004
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The cost of antibiotic prophylaxis sulbenicillin versus coamoxiclav (in term of medication use and treatment of complications) to treat cesarean section was compared.The medical records of patient receiving intravenous sulbenicillin orcoamoxiclav for the treatment of cesarean section in PKU Muhammadiyah Hospital were retrospectively reviewed. Data were collected for patients treated from January 1, 2000 to December 31, 2000. Patient data collected included patient data based, indication of cesarean section, abdominal incision and oral antibiotics. Cost data collected included drug acquisition cost, cost of drug supplies, nursing time to administer the agents and cost of managing complication.The medical records of 98 patients were identified and reviewed. The average length of stay was 4.85 days for the sulbenicillin group and 4.90days for the coamoxiclav group. The average total including the cost of complication was Rp. 140.509 in sulbenicillin group and Rp. 376.310 in coamoxiclav group.In conclusion, the study demonstrates that intravenous coamoxiclav has a higher cost than that of intravenous sulbenicillin. This result can be used to assist institution, clinicians and pharmacists in determining the most appropriate and efficient use of drugs. These data can be a powerful tool to support various clinical and policy drug use decisions, for example included formulary management and drug use policy or guidelines.Key words: antibiotics, cesarean section, cost.

RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF FINASTERIDE AND TAMSULOSIN IN TABLET FORMULATIONS Ashu Mittal; Shikha Parmar; Syed Sarim Imam; Sadaf J. Gilani; Mohd Aqil
Indonesian Journal of Pharmacy Vol 26 No 1, 2015
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The object of the present study was to develop a simple, precise simultaneous HPLC method for estimation of Tamsulosin (TAM) and Finasteride (FIN) in bulk and pharmaceutical dosage form. The method involves the use of easily available inexpensive laboratory solvents. The separation was achieved on spherisorb C-18 column with isocratic flow detected at 210nm. The mobile phase consisted of methanol: 0.03mM phosphate buffer pH 3.5 (70:30v/v) with flow rate of 1.0mL/min. A linear response was observed over concentration range of 4-40μg/mL for tamsulosin and 10-80μg/mL for finasteride. Limit of detection and limit of quantitation for tamsulosin were 1.13μg/mL and 3.43μg/mL, and for finasteride were 1.37μg/mL and 4.21μg/mL, respectively. The recovery of tamsulosin and finasteride was found to be in the range of 98.80-100.24% and 99.33-100.33%,respectively. The low %RSD value indicated a good precision and stability of the analytical method. The analysis concluded that the method was selective for simultaneous estimation of tamsulosin and finasteride can be potentially used for the estimation of these drugs in combined dosage form.Key words: HPLC, tamsulosin, finasteride, validation, pharmaceutical dosage form

Effect of a selective GABAB receptor agonist, baclofen, on the opioid-induced antinociception and rewarding effect Arief Nurrochmad; Ozaki Masahiko; Yoshinori Yajima; Minoru Narita; Tsutomu Suzuki
Indonesian Journal of Pharmacy Vol 16 No 2, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The management of excessive adverse effects is a major clinical problem. Multiple approaches have been described to address this problem. Successful pain management with opioids required the adequate analgesia without excessive side effects. The present study was designed to investigatethe effect of a selective GABAB receptor agonist baclofen on the opioid induced antinociception and rewarding effect. In the present study, we confirmed that either morphine or fentanyl produced a dose dependent antinociceptive effect in mice using tail-flick test. The results demonstratedthat co-administration of baclofen with morphine, fentanyl or oxycodone produced the synergistic effect on antinociception in mice. In the place preference study, we found that baclofen inhibited on morphine or fentanylinduced place preference in rats. These results suggest that coadministrationof baclofen with opioids produce synergistic antinociception with less effects of place preference We propose here that co-administration of baclofen with opioids may pave the way for the new strategy for the control of pain and recommended for the adjuvant drug.Key words : opioid, place preference, rewarding effects, baclofen

DEVELOPMENT AND CHARACTERIZATION OF POLYCLONAL ANTIBODY OF RECOMBINANT HUMAN INTERFERON Α2B IN NEW ZEALAND WHITE RABBIT Heni Rachmawati
Indonesian Journal of Pharmacy Vol 25 No 3, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

We have developed recombinant wild type and mutant human interferon α2b (rhIFNα2b) from synthetic gene in Escherichia coli. To identify the successful product of the proteins, immunology-based assay was suggested due to specificity for characterization. This work was aimed to develop and characterize rhIFNα2b polyclonal antibody generated in White New Zealand rabbits. The rhIFNα2b was overproduced in Escherichia coli BL21 containing rhIFNα2b synthetic gene in pET32b.The protein was obtained as inclusion bodies, refolded, purified using nickel affinity chromatography, and characterized using polyacrylamide gel electrophoresis. The purified rhIFNα2b protein was injected into rabbits for 21 days. Absorption of E.coli antibody was done using total E. coli protein to remove antibody againts host cell. The generation of antibody was monitored using dot blot and Western blot methods and quantified using Enzyme Linked Immunsorbant Assay (ELISA). To do so, rhIFNa2b was used as an antigen. The result showed that the rhIFNα2b was produced as a His-tag protein fusion of 33kDa in size. The results of dot blot and Western blot analyses strongly indicated that antibody against rhIFNα2b was generated and specifically recognized rhIFNα2b. ELISA showed that the titer of the polyclonal anti-rhIFNα2b was 1:10.000. In conclusion, polyclonal antibody spesifically against rhIFNα2b protein was successfully detected with high titer after 21 days after rabbit immunization.

CYP1A1 and GSTm expression of hepatocytes induced by 7,12-dimethylbenz(a)anthracene and the influence of ethanolic extract of Gynura procumbens Iwan Sahrial Hamid; Sugiyanto .; Edy Meiyanto; Sitarina Widyarini
Indonesian Journal of Pharmacy Vol 20 No 4, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The cytochrome P-450 (CYP) and glutathione S-transferase (GST) enzyme systems may influence the biological effects of carcinogens. As such, these enzymes may predict the developmental risk of breast cancer, as well as be potential targets for chemoprevention. The purpose of this study was to compare the expression of CYP1A1 and GST* between treatment groups. Expression of CYP1A1 and GST* was quantified in liver tissue from 18 female Sprague Dawley rats aged 40 days, which randomly divided into six treatment groups. Those are base line group (without DMBA and ethanolic extract treatment), DMBA induced cancer group, the other two groups was administrated by DMBA after treated by ethanolic extract in two different doses, 300 mg /kg BW and 750 mg/kg BW. The last two groups were given two doses of extract ethanolic only, without initiated of DMBA. The ingestion of the extract was carried for three weeks and the ingestion of DMBA was performed twice in the third week. The expression CYP1A1 and GST was quantified by immunohistochemistry. CYP1A1 expression was significantly higher (P < 0.05) in cancer group (DMBA) as compared with other groups. On the other hand, GST expression was lower in cancer group (P < 0.05). Result of this study demonstrated that ethanolic extract leaves of G. procumbens in 300 mg/kg BW dose could inhibit CYP1A1 stronger than are other and induced GST* level. Has effect on ethanolic extract leaves of G. procumbens has ability in played role of as blocking agent in preventing initiation stage of carsinogenesis, therefore it should be taken into account for chemopreventing agent in mammary carsinogenesis.Key words : Gynura procumbens, breast cancer, Chemopreventive, CYP1A1, GSTμ

THE EVALUATION OF SINGLET OXYGEN QUENCHING AND SUNSCREEN ACTIVITY OF CORN COB EXTRACT Suryanto, Edi; Momuat, Lidya Irma; Yudistira, Adithya; Wehantouw, Frenly
Indonesian Journal of Pharmacy Vol 24 No 4, 2013
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The corn cob waste has been reported to have antioxidant activity. Active compound such as antioxidant has been considered as potential sunscreen resources. The objectives of this research were to determine singlet oxygen quenching and sunscreen activity of corn cob. Corn cob was extracted using ethanol 20, 40, 60 and 80% by reflux for 2h at 78oC. The singlet oxygen quenching activity was evaluated by photooxidation of linoleic acid. Singlet oxygen quenching activity was conducted using linoleic acid as substrate containing erythrosine as a sensitizer and exposed under continuous illumination (4000 lux) with the fluorescent light for up to 5h. The sunscreen activity was evaluated by sun protection factor (SPF) using spectrophotometry UV-Vis. Ethanol extract 80% (E80) shows the highest total phenolic content followed with E60, E40 and E20. The result shows that the lowest singlet oxygen quenching activity was E20 (15.63%), and the highest was E80 by 65.63% percentage of inhibition. SPF value of E20, E40, E60, and E80 at concentration 200μg/mL were 7.52; 12.24; 15.81 and 17.78, respectively. SPF value increase with the increasing of concentration, total phenolic content and singlet oxygen quenching activity. The conclusion of this research was corn cob extract possess phytochemical compound having potency as singlet oxygen quencher and sunscreen active compound.Keywords: corn cob, phenolic phytochemical, singlet oxygen quenching, sunscreens

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