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INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 986 Documents
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Growth Rate Differences of Chrysomya sp. Larvae on Rattus novergicus Wistar Strain Corpse Exposed and Unexposed to Ephedrine Toxic Dose Poeranto, Sri; Prastowo, Wening; Resmi, Diandra Laksmita; Nugraha, Rivo Yudhinata Brian
Journal of Tropical Life Science Vol 7, No 3 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.03.06

Abstract

Post Mortem Interval (PMI) is used as a parameter to define the onset, cause, manner, and time of death to help maintenance of law and identify crime victims by the age of the larvae found. Larval growth is influenced by the temperature, humidity, and contaminant (drug or toxic). This experiment used two media of dead rats (200 grams), one given ephedrine of LD50 = 266 mg/kg and the other without ephedrine. Both were put in 2 cages each containing 50 Chrysomya sp. Ten larvae were randomly taken every day for 14 days to be measured their length, weight, and duration of growth. The 3rd larvae stage in the media with ephedrine had weight gain on 5th – 6th day morning, while larvae in media without ephedrine had static weight gain on 5th day morning - afternoon and weight decrement on 6 – 7th day morning. Larva’s peak ratio of length/weight in the media with ephedrine was higher than that of larvae in the media without ephedrine. Larvae on media with ephedrine grew faster in 1st, 2nd, 3rd larvae stage, and pupal stage compared to larvae without ephedrine. Therefore, this study indicates that ephedrine can accelerate the growth rate of Chrysomya sp. larvae.
Gynura procumbens Ethanolic Extract Promotes Lymphocyte Activation and Regulatory T Cell Generation In Vitro Dwijayanti, Dinia Rizqi; Rifa'i, Muhaimin
Journal of Tropical Life Science Vol 5, No 1 (2015)
Publisher : Journal of Tropical Life Science

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Abstract

Immune system is a system of biological structures and processes within organism directed to protect against invaded pathogen. Cellular and humoral immune system mediated by immunocompetent cells such as CD4+ T cells, CD8+ T cells, CD4+CD25+ T cells, and B220 cells play important role for maintaining immunological surveillance. The purpose of this study was to determine the effect of ethanolic extract of  G. procumbens leaves (EEGL) on the profile of CD4+ T cells, CD4+CD25+ T cells, and B220+ cells. Splenic cells were isolated from BALB/c mice and cultured in RPMI1640 medium in the presence of EEGL. After 4 days of incubation, cells were harvested, stained with antibodies and analyzed by flow cytometer. The data were analyzed by one-way ANOVA with α= 0.05 and Tukey test using SPSS 16.0 for windows. The results showed that the extract of  G. procumbens could increase proliferation of CD4+CD62L T cell, CD4+CD25+T cells, and B220+ cells compared to the control. Here, we showed the biological effect of G. procumbensas medicinal herb with immunomodulatory activity andthe dose of 0.1 µg/ml and 1.0 µg/ml could promote T cell activation compared to the highest dose of 10 µg/ml. In terestingly, the dose of 10 µg/ml rather promote than inhibit B cell proliferation.
Hydro- Chemical Evolution of Palk Strait Region, Bay of Bengal govindasamy, chinavenkataraman; Arulpriya, Mani; Ruban, Packiasamy; Meenakshi, V. R.
Journal of Tropical Life Science Vol 2, No 1 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

Present analysis was carried out the physico- chemical parameters of two stations such as Thondi and Soliyakudi in the southeast Palk Strait region during the monsoon and post monsoon seasons (November 2008 – March 2009). High temperature (31ËšC) and salinity (35%o) was recorded in the month of March (post monsoon). Dissolved oxygen content was high (5.2 O2 mg. l-1) during in the monsoonal season. Compared with other nutrients such as NO3, NO2 and SiO2, the total phosphorous concentration was high in both the stations during monsoonal season. In general concentrations of all the nutrients were high in the station I during monsoon season (November and December) which could be due to the addition of nutrients through the sewage, agricultural and land run off.
Effect of Methyl Parathion on Survival and Development of Tadpoles of Indian Cricket frog Fejervarya limnocharis Puttaswamy Gowda, Gurushankara H; Krishnamurthy, Sannanegunda Venkatarama Bhatta; Venkateshaiah, Vasudev
Journal of Tropical Life Science Vol 6, No 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.08

Abstract

Amphibian populations are declining due to various causes including pesticide contamination in natural habitat. We evaluated effect of Methyl Parathion (MPT) an organophosphate pesticide on survival and development of common paddy field frog Fejervarya limnocharis in a laboratory condition. Effect of 0 µg MPT/L, 500 µg MPT/L, 1000 µg MPT/L, 1500 µg MPT/L, 2000 µg MPT/L and 3000 µg MPT/L was studied using static toxicity test for a duration of 28 days. MPT reduced the survival of tadpole. The mortality increased with the increased concentration of pesticide. The development decreased with increased MPT concentrations. At higher concentrations, MPT induced slow development and tadpoles failed to metamorphose. It is assumed that slow development could affect the larval life and amphibian population in agro-ecosystem.
Observation of Frog Species in State University of Malang as a Preliminary Effort on Frog Conservation Wulandari, Dian Ratri; Habibi, Muhammad; Listyorini, Dwi
Journal of Tropical Life Science Vol 3, No 1 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.3.1.%x

Abstract

Frog is an amphibian which widely distributed around the world. Indonesia houses 450 species which represent 11% of frog species in the world. In Java Island living 42 species of frogs and toads. Frogs can be used as an environment indicator due to the presence of frog in a particular place indicates that the place is stay natural and unpolluted. State University of Malang Campus #1 which is located in the heart of Malang District has been developing rapidly, currently. Thus, it requires for the construction of new various facilities to support it widen activities. Extensive construction can be destructive even damaging to the habitat of frog, which potentially threat the frog life, if it does not take a consideration on the environmental impact, carefully. This study aimed to identify the species of frog which is survives in State University of Malang with consideration of the frog species found in 1995. Species identification conducted by observing the morphologicalcharacter. This study found four species with three species which are survive and as found in 1995; those are Duttaphrynus melanostictus, Polypedates leucomystax, and Kaloula baleta; and one new species that is Rana chalconota. This study also reveals there are four species which are extinct; those are Fejervarya cancrivora, Fejervarya limnocharis, Ingerophrynus biporcatus, and Occidoziga lima. This situation shows the decreasing amount of species from 7 to 4 within 17 years. This result indicates that there is a serious environmental degradation which caused the losing of frog habitats. Further research is needed to study the ecological condition changing in order to save the frog species.
Aspleniaceae of Tandikek Mountain West Sumatra Mildawati, Mildawati; Arbain, Ardinis; Fitrah, Hary
Journal of Tropical Life Science Vol 3, No 3 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

Research on ferns of Aspleniaceae family of Tandikek Mountain in West Sumatra, Indonesia has been done through a survey method and direct collection in the field, followed by a study at Herbarium of ANDA (Andalas University). Eleven species consisting of Asplenium affine, A. batuense, A. belangeri, A. pellucidum, A. phylitidis, A. robustum, A. salignum, A. scalare, A. tenerum, A. unilateralis, and Asplenium sp1. have been found as part of the Asplenium genus. The species of Asplenium genus have been obtained from the elevation of 1231 - 2336 meter above the sea level.Keywords: Aspleniaceae, Tandikek ountain, West Sumatra
Botanical Survey in Moyo Island, West Nusa Tenggara, Indonesia: Inventory of Flora Collection at Forest Trimanto, Trimanto; Siahaan, Febrina Artauli
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.12

Abstract

Nusa Tenggara consists of some small islands, one of them is Moyo Island. The diversity of plant species in this island is not really known for certain. This research was determined to observe the diversity of plant spe-cies in Moyo Island forest. The research was conducted in April 2013 by using floristic analysis method. The characteristic of Moyo Island forest is lowland evergreen rain forest. The results showed there were 60 tree species recorded in Moyo Island forest. There were many fruiting trees and seedling from the trees which show healthy growth, indicated that the plant regeneration in this forest is went well. The diversity of Pteri-dophytes and orchids were not high. Epiphytic fern which often found in the forest were Drynaria quersifolia and Platycerium bifurcatum and terrestrial orchid that dominated in the forest was Nervilia aragoana. Tuber plant was often found in this forest and grew prolifically were Tacca, Dioscorea and Amorphophallus. In coastal area lived a population of Pandanus tectorius. There were three new record plants found. The first was epiphytic orchid: Pteroceras javanica, the second was the epiphytic plant: Hoya verticillata and wild tuber plant: Tacca leontopetaloides.
Potential Anti-cancer and Anti-bacterial Activities of Philippine Echinoderm Extracts Layson, Rodyl J.; Rodil, Maria Criselda A.; Mojica, Elmer-Rico E.; Deocaris, Custer C.
Journal of Tropical Life Science Vol 4, No 3 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Blooming Stimulation of Microcystis in Sutami Reservoir Using Nutrients Nitrate and Phosphate in Different ratio Retnaningdyah, Catur; Suharjono, Suharjono; Soegianto, Agoes; Irawan, Bambang
Journal of Tropical Life Science Vol 1, No 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Microalgae Cyanobacteria from the genus Microcystis has the potential to bloom in Sutami reservoirs that located in Malang, Indonesia at a particular time. Microcystis is a notorious species because it can produce toxins that are dangerous to other organisms. The objective of this research is to determine the influence of different ratios of Nitrate and Phosphate on the growth of Microcystis in Sutami reservoir. The study was conducted from April to November 2009 and was carried out in situ in the reservoir Sutami with quasi experiment using a completely randomized design. Sutami reservoir water which already contained plankton community therein, including Microcystis were treated with five variations ratio of Nitrate and Phosphate (10, 20, 40, 80, and 160) and untreated water as control. Experiments carried out by using a transparent plastic bag diameter 1 m and 1.5 m long, inserted into the water and be immersed in the water by using bamboo. Calculation of Microcystis abundance and physic-chemical quality of water is done every three days for a month. The results showed that the higher concentration of orthophosphate in the media water of Sutami reservoir have the maximum abundance of Microcystis. Abundance of Microcystis grown in Sutami reservoirs have positively correlated with actual levels of nitrate, nitrite, total phosphate, conductivity, pH, temperature, ratio of Nitrate to Phosphate treatment and water KMnO4 value.Keywords: Blooming Microcystis, the ratio of nutrients Nitrate to Phosphate, Sutami reservoir
Cocoa extract has activity on selectively killing of breast cancer cells line tunjung-sari, ariza budi; Mahriani, Mahriani; Tiningrum, Gusti Agung Perias; Wahyudi, Teguh; Jati, Misnawi
Journal of Tropical Life Science Vol 5, No 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.04

Abstract

Effect of the cocoa crude extract on mortality of breast cancer cell lines i.e. MCF-7, T47D and normal cell (Vero), was observed. Crude cocoa extract prepared from a freshly dried cocoa bean that was containing 14% catechin and 0.6% caffeine. Catechin and caffeine content were modulated to 2-folds (28% catechin or 1.2% caffeine) and 3-folds (42% catechin or 1.8% caffeine) by adding pure compounds. Extracts were dissolved in dimethylsulfoxide (DMSO) at concentrations ranging from 200 to 1600 μg/ml. The positive control was doxorubicin (0.5-16 μg/ml in DMSO). Cell lines (MCF-7, T47D, and Vero) were incubated in test sample for 24h at 37°, prior to 3-(4,4-dimetylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The absorbance of each well was measured at 550 nm, and lethal concentration (LC50) was calculated. The cocoa extract induced mortality of breast cancer cell lines but not in Vero cells. The effect on MCF-7 was greater than on T47D, given the LC50 was 1236 μg/ml (MCF-7) and 1893 μg/ml (T47D). Cytotoxic potential of cocoa extract was much lower than doxorubicin whose LC50 was0,777 μg/ml (MCF-7) and 0,082 μg/ml (T47D). Increasing catechin content to 2-folds did not significantly affect LC50 value, but 3-folds catechin content reduced LC50 to 1021 μg/ml. Meanwhile increasing caffeine content to 2-folds significantly reduced LC50 to 750 μg/ml, however, 3-fold content resulted in slightly higher LC50 at 780 μg/ml. This indicates that cocoa extract have anti-cancer potential, and purification may improve this property .

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