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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 986 Documents
Selection of High Oil Yielding Trees of Millettia pinnata (L.) Panigrahi, Vegetative Propagation and Growth in the Field Arpiwi, Ni Luh; Sutha Negara, I Made; Simpen, I Nengah
Journal of Tropical Life Science Vol 7, No 3 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.03.11

Abstract

Millettia pinnata (L.) Panigrahi is a potential legume tree that produces seed oil for biodiesel feedstock. The initial step for raising a large-scale plantation of the species is selection of high oil yielding trees from the natural habitat. This is followed by vegetative propagation of the selected trees and then testing the growth of the clone in the field.  The aim of the present study was to select high-oil yielding trees of M. pinnata, to propagate the selected trees by budding and to evaluate the survival and growth of budded plants in the field. Pods were collected from 30 trees in Lovina Beach, Buleleng Regency, Bali. Oil was extracted from seeds using soxhlet with hexane as a solvent.  The high oil yielding trees were propagated by budding using root stocks grown from M. pinnata seeds.  Scions were taken from young branches of selected trees. Incision was made on rootstock and the same size of cut was made on a scion containing a single bud.  The scion was inserted to the incision of rootstock then closed tightly using plastic strips.   The plastic was removed when the scion grew into a little green shoot. One month after plastic removal, the scion union grew into a single shoot and then the budded plants were removed to polybags. Budded plants were planted in the field of Bukit Jimbaran, Badung Regency, Bali with 4 × 4 spacing. Results showed all budded plants successfully grow new shoots. Two months after planting the survival of budded plants was 100%. Plant height increased by 22.13 cm, stem diameter increased by 2.43 mm and the number of compound leaf increased by 2.08.  It can be concluded that four high oil yielding trees were selected from Lovina Beach and successfully propagated by budding. Survival of budded plants was 100% with vigorous growth.
The Pathophysiology and Care of Exercise Related Muscle Cramps Pandey, Abhay Kumar; Pandit, Abha; Pandey, B L
Journal of Tropical Life Science Vol 5, No 1 (2015)
Publisher : Journal of Tropical Life Science

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Abstract

Cramps are major concerns to competing athletes. The pain alone is not object of treatment as serious metabolic disturbance of electrolyte must gets due consideration. The phenomenon is dealt with some patho-physiological details and relevant clinical information, important as first hand understanding for the sports persons and their care givers.
Ethno-Edible Mushroom of Pleurotus sp., Clytocybe nebularis and Auricularia auricula in Ranupani Village, East Java Haryati, Jehan Ramdani; Azrianingsih, Rodiyati
Journal of Tropical Life Science Vol 2, No 2 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

This paper documented the knowledge about the wild edible fungal flora of Ranupani Village of East Java Indonesia that used by indigenous Tenggerese tribes. Study was conducted by using semi-structured interview and purposive sampling. Data were analyzed quantitatively descriptive. Index of Cultural Significance (ICS) was used to evaluate the importance of non-wood forest yields for local people. Identification of edible mushrooms was based on Guidelines of Flora Diversity Data Collection. This study was focus on the three edible mushrooms which are eaten mostly by the villagers, i.e. Pleurotus sp., Clytocybe nebularis and Auricularia auricula. The result is the Ranupani residents perception of edible mushrooms and its potential as an alternative source of food based on local knowledge and local wisdom of Tenggerese. Pleurotus sp. has the highest preference and intensity to be consumed with 3,2 ICS. It means culturally, this edible mushroom were not too important but intensively used as a secondary food sources such as soup (added or sauted), chips (dried) and fried. Ranupani Villagers always consumed edible mushrooms in rainy season. The ability of residents to distinguish edible mushrooms are equal to the searching ability to find edible mushroom substrate. The residents will recognized the substrate of edible mushrooms on dead trunks of Pasang Tree (Lithocarpus sundaicus), Danglu (Engelhardia spicata), Kemlandingan (Albizia Montana), Casuarina (Casuarina junghuhniana) and acacia (Acacia decurens). Residents who do not have the ability to distinguish and searching were consumer, get the edible mushrooms by buying from the searcher or distributor in packs. The characteristics of an edible mushrooms are a discrete soft flesh of the fruit body, dark color, no ring on the stipe, the presence of insects (e.g. moths) in the lamellae) and the type of mushroom substrate. There were also assisted growths of wild mushrooms by the residents’ raw chop the dead tree trunk as a substrate deliberately. Respondents who knew the time of harvesting edible mushroom were 58.3%. Pasang mushrooms (natural & assisted growth) can be found throughout the rainy season, with an interval of 12 days for subsequent retrieval. While Barat and Kuping mushrooms are found throughout the rainy season, but randomly distributed in the forest. There were unwritten but spoken hereditary local wisdom for the sustainable of the edible mushrooms in Tengger. Only Tenggerese allowed collecting and selling the edible mushrooms in Tengger area to the Tenggerese themselves and tourist (local or foreign). This wisdom is supported by TNBTS regulation that the edible mushroom were not allowed exported out of TNBTS area. This wisdom expectedly makes sure that the edible mushrooms should be sustained in the forest. Keywords:Ethno-edible mushrooms, Ranupani
Botanical Exploration and Crater Vegetation Survey of Mt. Galunggung, West Java Zuhri, Musyarofah; Wiriadinata, Harry; Astuti, Ratna Suti; Hadiwaluyo, Supan; Syamsudin, Syamsudin
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.02

Abstract

Botanical exploration at Mt. Galunggung protected forest, West Java was conducted in June 2014. This study was aimed to collect plant living specimen for ex-situ conservation purpose of Kuningan Botanic Garden and to conduct vegetation analysis in crater area. Random inventory method at 600-1200 m asl was used to collect plant in that area. Vegetation analysis of tree, shrub and herb were used line transect methods. Result showed that 273 collection numbers of plant living species were collected from Mt. Galunggung, which consisted of 213 species, 143 genus and 72 families. Plant families which most collected were Moraceae, Euphorbiaceae, Arecaceae and Lauraceae respectively. In crater area was collected a lot of pioneer plant, such as dawola (Trema cannabina Lour.), paku tiang (Cyathea spp.), nangsi (Villebrunea rubescens (Blume) Blume), muncang cina (Ostodes paniculata Blume), mara (Macaranga tanarius (L.) Mull.Arg.), and kareumbi (Homalanthus populneus (Geiseler) Pax). Furthermore, in secondary remnant forest were collected typical plants of Java mountains such as saninten (Castanopsis argentea (Blume) A.DC., puspa (Schima wallichii Choisy) and some of tree seedlings from Lauraceae family. Vegetation affected by eruption in Mt. Galunggung is in early stage of forest succession and still develops to next stage of succession. Tree, shrub and herb in the crater area were dominated by Cyathea contaminans (Wall. Ex Hook.) Copel., Calliandra callothyrsus Meisn. and Ageratina riparia (Regel) R.M.King & H.Rob. with H’ was 0.35, 0.31 and 0.30 respectively.
Discussing implications of fast depleting rural ponds on the globally threatened wetland winter migratory bird in Haryana: a Case Study of Nigdu village pond in Karnal District Gupta, Rohtash Chand; Kaushik, Tirshem Kumar
Journal of Tropical Life Science Vol 3, No 2 (2013)
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Abstract

Spatial Distribution and Genetic diversity of Tripneustes gratilla in Ambon Island Silahooy, Veince Benyamin; Hakim, luchman; Toha, Hamid; ., widodo
Journal of Tropical Life Science Vol 3, No 3 (2013)
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Abstract

Tripneustes gratilla an important ecological role in various habitats because T. gratilla is directly or indirectly recycling key from the nutrients in the waters of Ambon Island. Ambon Island has a distinctive topography, thus causing inhibition of water exchange period naturally in Ambon Bay and feared to complicate the exchange of genes between populations thus causing inbreeding. In addition the increasing environmental pollution can narrow the habitat of Tripneustes gratilla. Therefore, this study aims to determine the spatial distribution and genetic diversity of sea urchins (Tripneustes gratilla) on Ambon island based on cytochrome c oxidase subunit 1 gene. Alignment results showed the presence of genetic variation of T. gratilla on the Ambon island. This is shown in several polymorphic base where there has tranversi experience, transition, and insertion. COI gene sequence similarity values of T. gratilla on Ambon island is around 98.4 to 100%. Distribution of T. gratilla population found on Ambon island generally have a relatively small number of individuals. From four locations that just only one location have large enough number of individuals. However, the results of genetic analysis using COI gene showed the presence of genetic closeness between T. gratilla despite located in different locations.
The Classification of Bambusa spp. from Celebes Based on the Micromorphological Characters of Leaf Epidermis Liana, Alin; Purnomo, Purnomo; Sumardi, Issirep; Daryono, Budi Setiadi
Journal of Tropical Life Science Vol 7, No 3 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.03.02

Abstract

Species of Bambusa had widespread in Celebes, especially for Bambusa striata and Bambusa vulgaris. As an effect of the lacking of flowering, species identification mainly depends on vegetative features, and leaf epidermal micromorphology has proven to be useful in bamboo taxonomy. The objective of this research was to describe the classification of Bambusa from Celebes based on the micromorphological characters of leaf epidermis. The specimens were collected from wild population. The samples of leaf were collected from five member of Bambusa i.e.: Bambusa blumeana, Bambusa maculata, B. striata, B. vulgaris and Bambusa sp. Micromorphological characters were identified using Scanning Electron Microscopy (SEM). Leaf epidermis characters separated B. blumeana from other species of Bambusa. Furthermore, B. striata were closely related to B. maculata in variation of bulliform cells. As an invention, we release peltate hair as a new type of trichome in Bambusa. The presence of various prickles, trichomes and bulliform cells may be especially useful in delimiting species.
Bovine And Human Zona Pellucida 3 Gene Glycans Site Prediction Using In Silico Analysis Mubarakati, Nurul Jadid; Aulanni'am, Aulanni'am; Sumitro, Sutiman Bambang; Ciptadi, Gatot
Journal of Tropical Life Science Vol 4, No 3 (2014)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.04.03.08

Abstract

Zona pellucida is one of the protective layer of the egg cell and has a function as an intermediary species-specific fertilization. Glycoproteins of human and bovine zona pellucida is composed of three types, namely ZP1, ZP2 and ZP3. ZP3 gene has amino acid sequence homology with other mammals. Oligosaccharides components of the zona pellucida glycans are composed from units of asparagine residues (N -linked) and serine/threonine (O -linked). The aims of this study was to analyze the DNA sequences of human and bovine and further predicts glycans site on amino acid sequence of human and bovine ZP3. In this study, ZP3 gene fragments have been isolated from bovine and humans were analyzed in Silico. This work were conducted by comparing the data of DNA sequence from human and bovine PCR product using NCBI BLAST. The results showed that there were similarities at amino acid positions number 23-38. bZP3 sequence had three glycans site (Asn-X-Thr/Ser) and one site on hZP3 glycans. One of the sites was conserved between the two species.
Effect of Dietary Amorphophallus sp From East Java on LDL-C Rats (Rattus novergicus Wistar Strain) Harijati, Nunung; Widyarti, Sri; Azrianingsih, Rodiyati
Journal of Tropical Life Science Vol 1, No 2 (2011)
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Abstract

One of indication of obesity is high LDL-C . Obesity has serious risk to health, it can cause heart disease and stroke. Effort to lower obesity using drugs have significant side effects such as insomnia, increased blood pressure, dry mouth and so forth. Therefore using natural products that contain glucomannan to reduce obesity and LDL-C is good choise. Glucomannan in the global market derived from Amorphophallus konjac. In this study, we used glucomannan from Amorphophallus endemic East Java to reduce fattened Wistar rats. Amorphophallus that used include Amorphophallus muelleri, A.variabilis (variant: Brongkos 32; Brongkos 5; Wonorejo, Brangsi) and Amorphophallus campanulatus (Var Selopuro). Amorphophallus diet was given to white rats of Wistar strain that previously fattened using a mixture of cholesterol and lard, in addition to PARS (Chicken Feed Race Super) as a basic food. Amorphophallus konjac was used as control. Measurement of LDL-C was conducted in four different time points, namely 1). Prior to dietary cholesterol, 2). 24 days after the dietary cholesterol, 3) 11 days after Amorphophallus (+cholesterol) diet, 4). 25 days after Amorphophallus (+cholesterol) diet. The results showed that the diet of A.variabilis potentially lowered blood cholesterol levels for their respective 22.98%, 5.85% and 7.37% for consecutive variant Brongkos 32; Brongkos 5; Wonorejo. Diet from A.campanulatus and A.konjac had not been able to reduce cholesterol to the end of observation (25 days).Keywords: LDL-C, A.variabilis, A.muelleri, A.campanulatus, A.konjac
Apigenin, an Anticancer Isolated from Macaranga gigantifolia Leaves Fajriah, Sofa; Megawati, Megawati; Darmawan, Akhmad
Journal of Tropical Life Science Vol 6, No 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.02

Abstract

Apigenin, a flavonoid compound has been isolated from the ethyl acetate fraction of methanol extract of Macaranga gigantifolia leaves. Isolation and purification of apigenin conducted using chromatographic method, and chemical structure characterized based on spectroscopic data. In vitro anticancer activity test against murine leukemia P-388 cell line showed potential activity with IC50 14.13 μg/mL.Key words: apigenin, flavones, Macaranga gigantifolia, anticancer, murine leukemia P-388 cell line.

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