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INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 986 Documents
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White Kabesak (Acacia Leucophloea RoxB) Leaves Utilization in Concentrate on Fermentation Products and In Vitro Gas Production Lawa, E.D. Wie; Chuzaemi, S; Hartutik, Hartutik; Marjuki, Marjuki
Journal of Tropical Life Science Vol 10, No 3 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aimed to evaluate gas production and in vitro fermentation products from feed containing leaves of white kabesak (acacia leucophloea Roxb.)Willd. Feed was composed of a ratio of 60% natural grasses and 40% concentrate. The treatments were used levels of white kabesak leaves in concentrates i.e. 0, 10, 20, 30 and 40% in the dry matter (DM) basis as treatments, R0, R1, R2, R3 and R4 treatments, respectively. The results showed that inclusion of acacia leucophloea leaves in concentrate increased organic matter and crude fiber contents but decreased the crude protein content. Increasing level of acacia leucophloea  leaves in concentrate decreased  gas production (b, ml / 500 mg DM) from 198.29 (R0) to 139.93 (R4). The gas production rate (c, ml /hour) was relatively constant between 0.034 to 0.036 on R0 - R3 and 0.028 on R4. Gas production at 48 hours incubation (y, ml / 500 mg DM) decreased from 153.38 (R0) to 103.23 (R4). The NH3 concentrations ranged from 6.17-7.31 mg / 100 ml and the total VFA was 83.07-91.96 mM. The lowest C2 / C3 ratio was in R2 (2.63). The highest IVDMD was 50.18-67.14% in R0 and the lowest IVOMD was 55.04-71.35% R4. The use of acacia leucophloea leaves at level 20% in concentrates as supplements was more efficient in reducing gas production and in vitro fermentation products.
Conservation strategy of Anaphalis spp. in Bromo Tengger Semeru National Park, East Java Ade, Filza Yulina; Hakim, Luchman; Arumingtyas, Estri Laras; Azrianingsih, Rodiyati
Journal of Tropical Life Science Vol 11, No 1 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/tls.11.01.10

Abstract

The objective of the paper were to identify factors related to conservation strategy of Anaphalis in BTSNP. This study found internal factor related to strength includes the existence of species and value of Anaphalis spp., while the weaknesses include increase of habitat degradation and disturbance caused by tourism activities. The opportunities for conservation includes support of policy in biodiversity conservation and increase of ecotourism movement, while the threats aspect include less control of land uses changes and increase of mass tourism. The SWOT analyses recommends some significant strategy which are derived from combination of enhancing strength to optimizing opportunities, reducing weaknesses and optimizing opportunities, Enhancing strength to reduce threats and managing weaknesses to manage risk.  
ISOLATION OF THERMOPHILIC LIPASE PRODUCING BACTERIUM FROM HOT SPRINGS AT THE EAST COAST OF PENINSULAR MALAYSIA Abdul Hamid, Tengku Haziyamin Tengku; Abidin, Nur Asimah Zainal; Hasan, Nurmusfirah
Journal of Tropical Life Science Vol 11, No 1 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.01.01

Abstract

Hot spring is a natural habitat for thermophilic bacteria and is the major source of thermostable enzymes useful in industrial application. In Malaysia, the search for thermophilic organisms have been focused on hot springs especially on the west coast of the peninsular. In this work, lipase producing thermophilic microorganisms were isolated from east coast hot springs in the states of Pahang and Terengganu. All of the 7 isolates showed the ability to grow at 45°C and formed lipolytic zones on tributyrin agar plates. However, only isolate B2B2 and S1B4 were able thrive at higher temperatures of up to 65°C. Bacillus and Geobacillus species was found to be the dominant bacterium isolated from these hot springs. From La hot spring, isolate L1 (MT 645486), LH2, (MT645483), LH3 (MT645484), LH4 (MT 645485) and LH5 (MT 645487) were all closely related to Bacillus sp. (94 to 97%). Meanwhile, from Bentong and Sungai Lembing hot springs, isolates B2B2 (MT668631) and S1B4 (MT668632) were closely related to Geobacillus kaustophilus (98.5%) and Geobacillus thermoleovorans (98%), respectively. Strains from Geobacillus sp. were able to thrive at higher temperature and their thermostable lipases have properties useful for biotechnological applications.
Diversity of Foliar Endophytic Fungi in Artocarpus heterophyllus Lam. and Citrus reticulata Blanco of Tripura Bhattacharya, Sanchita; Debnath, Sanjit; Saha, Ajay Krishna
Journal of Tropical Life Science Vol 11, No 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.15

Abstract

Present investigation dealt with the isolation and diversity of foliar fungal endophytes from two fruit yielding plants of Tripura state. Seasonal distributions of endophytes in respect to fruiting and non-fruiting season in two host plants were also studied. Twenty one fungal strains along with one nonsporulating hyaline form were isolated as foliar endophytes from the two host plants. In both fruiting and non-fruiting seasons, from Citrus reticulata seven foliar endophytic fungi were isolated. Whereas from Artocarpus heterophyllus in both the season eleven fungal endophytes with one nonsporulating hyaline form were isolated. The relative frequencies of isolated fungi from both A. heterophyllus and C. reticulata in fruiting and non-fruiting season significantly differed among the sampling sites, whereas relative frequencies individual endophytic fungus showed no significant differences among various sampling sites.
Balur and Improving Quality of Life Jayanti, Gatra Ervi; Subagjo, Saraswati
Journal of Tropical Life Science Vol 6, No 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.07

Abstract

Balur treatment aimed to leach free radicals overwhelmingly produced in unhealthy body with rubbed over the human body. In Balur used some amino acid and herbal such as Bawang Sabrang (Eleutherine palmifolia (L.) Merr), Kopi Balur (Mixed Coffea arabica L. with Acetosal), Coconut Water (Cocos nucifera L.), Secang (Caesalpinia sappan L.) and “Divine” smoke from cloves (Syzygium aromaticum (L.) Merr. & L. M. Perry) and Tobacco (Nicotiana tabacum L). This is an observation research of Balur application in volunteers during four years at Rumah Sehat, Malang, Indonesia. The result suggested that Balur can improve quality of life  in male volunteers i.e. 61,54% for Cancer (39 volunteers); 80% for Tumor (20 volunteers); 86,36% for Diabetes mellitus (22 volunteers); 93,33% for Heart (15 volunteers); 92,86% for Autism (28 volunteers); 72,73% for Hepatitis (11 volunteers); 100% neuron disease for (2 volunteers); 93,62% for various of disease (94 volunteers). Moreover imrovement quality of life in female volunteers i.e. 72,31% for Cancer (65 volunteers); 88,57% for Tumor (35 volunteers); 75% for Diabetes mellitus (16 volunteers); 100% for Hemorrhoid (3 volunteers); 100% for stroke (3 volunteers); 40% for Cardiomegaly (5 volunteers); 66,67% for Autism (3 volunteers); 88% for various of disease (50 volunteers) and 100% for healthy (3 volunteers). Keywords: Balur, herb in Balur process, improving quality of life
Diversity of Pteridophytes in Mount Timolan Protected Landscape, Zamboanga del Sur, Philippines Cudal, Maricris G; Calimbo, Liberty Grace L; Jr, Diosdado Sumibay; Salise, Jaidel; Gomez, Jessa; Pagaling, Meraflor; Locson, Jasper; Cahayagan, Joel; Seno, Jernol; Daculio, Jahuel Nathan
Journal of Tropical Life Science Vol 11, No 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.05

Abstract

This study serves as the first generation research on the rich yet not poorly known ferns and lycophyte species of the Mount Timolan Protected Landscape (MTPL). A total of 151 species from 62 genera and 24 families were recorded from December 2019-May 2019. Overall, the species of MTPL respectively holds 14%, 40% and 71% of the recorded total species, genera and families of ferns and lycophytes in the Philippines. The most represented families in terms of the number of genera is Polypodiaceae (10 spp.), whereas, the most represented genera in terms of the number of species were Selaginella (11 spp.). The results of the study showed that Mount Timolan Protected Landscape harbours number of some endemic species (17 spp.), critically endangered species (1 spp.), endangered species (3 spp.), vulnerable species (5 spp.), threatened, non-native species and economically important species (25 spp.). Based on conservation and ecological status of assessed ferns and lycophyte species, it is likely to strongly suggest to set species-specific conservation management strategy to protect the threatened species. However, additional studies and thorough field explorations should also be done to conduct a re-inventory on the pteridophyte species in order to arrive a more scientific results to establish a real profile of the ferns and lycophyte species of Mount Timolan Protected Landscape.
Improvement of Trimethylamine Uptake by Euphorbia milii: Effect of Inoculated Bacteria Siswanto, Dian; Thiravetyan, Paitip
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.11

Abstract

In the last few years, a great emphasis has been placed on phytoremediation of indoor air pollution studies. However, limited work has been addressed to observe the bacteria potential to assist the phytoremediation process of trimethylamine (TMA). In this work, the ability of 4 different bacteria for individual TMA removal and IAA production were observed. In addition, the enhancement of TMA removal efficiency by Euphorbia milii with various inoculating bacteria were investigated. Bacillus thuringiensis, Citrobacter amalonaticus Y19, Bacillus nealsonii, and white colony-soil bacteria (WCSB) were able to absorb TMA and produce IAA individually. B. thuringiensis and C. amalonaticus Y19 were the two most effective bacteria to improve TMA removal efficiency by the plant. Since concentrations of IAA production by individual bacterium were highly correlated with TMA removal efficiency by plants in early periods of fumigation and highly correlated with leaf IAA production of bacterially inoculated plants, two predicted mechanisms on improving TMA uptake by bacterially inoculated plants are presented: (1) bacteria migration from plant roots to leaves increases leaf IAA concentration and (2) increasing concentration of bacterially inoculated root IAA inhibits transportation of IAA from leaves to roots, resulting in higher leaf IAA concentration. The higher concentration of leaf IAA is suggested to be a factor to increase stomatal opening which improves TMA removal efficiency of the plant.
Kinetic Parameters Analysis of Liver and Kidney Catalase Under The Influence of Cadmium and Mercury In Vitro Karantika, Ellsa Anggun; Supianur, Supianur; Edyson, Edyson; Suhartono, Eko
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.01

Abstract

Cadmium (Cd) and mercury (Hg) are toxic metals that affect human organs function, including liver and kidney. This toxic activity is because the heavy metal could induce oxidative stress and interfere antioxidant activities, including catalase (CAT). The present study was aims to evaluate the effect of Cd and Hg to liver and kidney CAT kinetic parameters in vitro. In this experiment, liver and kidney were taken from male rats (Rattus novergicus). Sample the homogenized and divided into three groups with; T0 served as control which contains liver or kidney homogenate + H2O2, T1 which contains liver or kidney homogenate + H2O2 + 0.03 mg/L CdSO4; and T2 which contains liver or kidney homogenate + H2O2 + 1 mg/L Hg. Solutions then incubated at 37ºC for 1 hour and then was prepared for CAT activity measurement. The CAT activity was measured using spectrophotometer at 240 nm. For measuring the kinetic parameters, different concentration of H2O2 were used. The kinetics parameters (Km and Vmax) were calculated using Lineweaver-Burk plot. The results shows that Cd and Hg could decrease the affinity of CAT-H2O2 complex which expressed by the higher Km and Vmax values. Also from the results, Cd has better activity to decreased the affinity of CAT-H2O2 complex than Hg. From this results, it can be concluded that Cd and Hg treatments could inhibit CAT activity in liver and kidney in vitro.
The expression of OsPLA2-III and OsPPO genes in rice (Oryza sativa L.) under Fe toxicity stress Turhadi, .; Hamim, .; Ghulamahdi, Munif; Miftahudin, .
Journal of Tropical Life Science Vol 11, No 2 (2021)
Publisher : Journal of Tropical Life Science

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Abstract

Lipid is an important biomolecule in plant because of their structural and functional roles in plant cells. Moreover, lipid could be a signal molecule in defense system of plants under biotic and abiotic stresses. Detoxification process of toxic substances, which is one of the defense system and controlled by various genes, plays an important role in tolerance mechanism in plant under stress conditions, including iron (Fe) toxicity. Plants develop various tolerance strategies to cope the Fe toxicity. The objective of this research was to investigate the expression of OsPLA2-III and OsPPO genes under Fe stress condition. Two-weeks-old seedlings of two rice varieties, i.e., IR64 (Fe-sensitive toxicity) and Pokkali (Fe-tolerant toxicity) were used. Both varieties were treated with 1 (control) and 400 ppm of Fe in the form of FeSO4.7H2O in the nutrient culture solution. The leaf bronzing, chlorophyll content, and relative expression of OsPLA2-III and OsPPO genes were observed. An in-silico study was also performed to predict the interaction between OsPLA2-III and OsPPO proteins. The results showed that the Fe toxicity induced leaf bronzing, reduced leaf chlorophyll content, and increased the OsPLA2-III and OsPPO gene expression levels. Both genes are suggested to have a role in plant tolerance mechanism during Fe toxicity stress via lipid signaling pathway.
Predominant Bacterial Diversity in Rheumatoid Arthritis Rat After Treated with Caprine CSN1S2 Protein Suyanto, Eko; Fatchiyah, Fatchiyah
Journal of Tropical Life Science Vol 11, No 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.01

Abstract

Introduction: Rheumatoid arthritis (RA) is an autoimmune and systemic inflammatory disease influenced by microbial abnormalities (dysbiosis) in the intestinal that affect changes in metabolism and immune system disorders. This study aimed to investigate the predominant intestinal microbiota in complete Freund’s Adjuvant-induced rheumatoid arthritis rats after treated with caprine milk CSN1S2 protein through fecal analysis based on PCR-DGGE and to construct the phylogenetic tree of bacteria as the evolutionary relationship. Method: The experimental animals were divided into 6 groups with 2 types of rat model, namely control rat (C group, CM group, and CY group) and RA rat (RA group, RAM group, and RAY group). Predominant cultivable microbiota was obtained by direct culture and analyzed using PCR-DGGE with some specific primers. The DNA sequences were analyzed and aligned using bioinformatics software to construct the phylogenetic tree. Results: We found that Lactobacillus group significantly increased in the control rat model and the predominant intestinal bacteria in RA rats were Enterococcus group (Enterococcus faecium and Enterococcus faecalis). Conclusions: The caprine milk CSN1S2 protein influences the composition of microbiota in RA rats with the emergence of predominant bacteria that are considered species of the Bacillus group, closely related to Bacillus coagulans, which can be promoted the growth of B. coagulans to suppress pathogenic bacteria in the development of RA disease.

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