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Articles 1,005 Documents
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Technical Functional Properties of Crude Water Soluble Polysaccharide From Durian Seed (Durio zibethinus Murr.) ., Herlina; Lindriati, Triana; Novijanto, Noer; Anggraini, Ayu
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Durian seeds can be utilized as a producer of Water Soluble Polysaccharide (WSP) that can be applied to food products because crude extract of WSP is hydrocolloid. WSP interacts with other components, directly and indirectly affect processing applications, food quality, and acceptance.Water binding, solubility, viscosity and surface activity are important properties, determining usefulness and final product quality in a food system. The absence of extraction conditions that support so that the need for improved methods to variations in temperature and long extraction. This study aim to determine the effect of temperature and long extraction that produces the highest yield value and to determine the functional properties of the WSP.  The results of this study are the highest yield values in the 50 oC temperature extraction ang long extraction 2 hours amounted to 2,26%, solubility in water of 51,61%; lightness 34,14; water holding capacity (WHC) 2339,35%;  oil holding capacity (OHC) 425,36%; emulsion power 55,77m2/g; emulsion stability 585,50 minutes, foaming power 157,39 ml/g; foaming stability 25,43%. Long extraction increase the value of WHC and OHC but discrease the value of water solubility, foaming power and lightness. Keywords: durian seed, water soluble polysaccharide, extraction, technical functional properties.
The Efficiency of GFP Gene Transformation on Peanut Embryo somatic Using Agrobacterium and Particle Bombardment Avivi, Sholeh; Dietzgen, Ralf G.; Higgins, Colleen M.; ., Sudarsono
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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The aims of this research were to (1) compare the effectiveness of established Agro bacterium mediated transformation and bombardment mediated transformation on peanut (2) evaluate the integration of green fluorescent protein (GFP) and hygromycin resistance (hygr) gene on the peanut embryosomatics. To achieve those objectives we shoot 1200 explants using bombardment and transform 1000 explants using Agrobacterium mediated transformation. Both of Agrobacterium mediated transformation and particle bombardment could give positive embryos transformed.  Particle bombardment gave 4.5% transformation efficiency while Agrobacterium mediated transformation gave only 1.8%. From this research, we concluded that particle bombardment more efficient and gave more transgenic explants than Agrobacterium mediated transformation. Keywords: agrobacterium, bombardment, GFP.
Dye-Sensitized Solar Cells (Dssc) Using Natural Dyes Extracted From Red Cabbage And Counter Electrode Based Tio2-Graphite Composites Haryati, Tanti; Mulyono, Tri; Oktavianawati, Ika; Badrianto, Wawan
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Dye sensitized solar cells (DSSC) has been a popular research topic which benefits in high energy conversion efficiency and less cost in fabrication process. The components of DSSC consist of photoanode, electrolyte, and counter electrode that are arranged as sandwich. The photoanode contains ITO (indium tin oxide) glass covered by TiO2 particles, while the counter electrode contains ITO glass covered by composite of TiO2-graphite as counter electrode and red cabbage extract, as a dye agent. The cell was fabricated in various amount of graphite, i.e 10%; 30%; and 50 %, and time of soaking i.e 2; 13; 24; and 48 hours. The performance of the solar cell per active area of ​​1 cm2 was tested on irradiation of 75 Watt halogen lamp (distance 15 cm) and measured by multimeter and rheostat. The optimum synthesis condition of DSSC was obtained with composition of TiO2:graphite on the counter electrode is 70%:30 %, and soaking time in dye agent is for 24 hours. This cell results in an optimum performance, Isc = 0.028 mA ;Voc = 0.344 mV, FF = 0.29 and = 0.0055 % efficiency. Keywords: DSSC, Red Cabbage, Counter Electrode
The Inhibitionof Bacterial Metalloenzymes and Fungal Protein Synthesis on Explants Surfaces by Sterilizing Agents Savitri, Wina Dian
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Surface sterilization is a very important key for reaching a successful explants introduction to the in vitro environment. However, to get the best protocol of explants surface sterilization is not easy. Each explants has its characteristics and the contaminants (which usually are bacteria and fungus) are vary depending on its source. In order to know the best sterilization protocol of Phaleria macrocarpa leaves, some sterilizing agents were tested. This experiment suggests that ethylene bis dithiocarbamate may eradicate fungal infection by metalloenzymes inhibition, which is showed by 91.7% survivals. In the other hand, streptomycin sulfate may eliminate bacterial infection by inhibition of protein synthesis, which is showed by 95.8% to 98.3% survivals depending on the exposure time. Keywords: ethylene bis dithiocarbamate, metalloenzymes, streptomycin sulfate, protein synthesis, sterilization
Molecular Dynamic Simulation for Thermal Stability Properties of Endo β-Mannanase Enzyme Yulandi, Adi; Hermosaningtyas, A A; Sutanto, Sheila; Suwanto, Antonius
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Endo β-mannanase or mannanase hydrolyse the β-D-1,4 mannopyranoside linkages in β-mannan intomanno-oligosaccharides. Four mannanases, named MAN1, MAN2, MAN3 and MAN4, were isolated from palm kernel meal waste have potential as thermostable mannanases. Series of enzymatic assay to characterize enzyme properties may affect longer time and higher cost. Homology modeling and molecular dynamic simulation are reliable and faster alternative assay to determine enzyme properties by analyzing enzymes’ three-dimensional structure. The structureswere constructed using homology modeling approach using Modeller. Template 2QHA was chosen for having more than 98% sequence similarity with targets. The homology models and template were simulated using molecular dynamics software GROMACS 4.6 for 10 ns production time each at 300 K, 323 K and 353 K. Both targets share the same (β/α)8 TIM barrel folding type similar to template 2QHA The basic analysis of molecular dynamic simulation (root mean square deviation and root mean square fluctuation) showed that both enzymes were thermostable, albeit compared to template 2QHA amino acid residues substitution in samples contribute for different thermostable profile. However, MAN2 was appeared to be more stable at high temperature than other samples. Keywords: endo β-mananase, homology modeling, molecular dynamic simulation
The Effect of Bitter Melon Extract (Momordica charantia)in Inhibition of NFkB Activation in Leptin treated HUVECS Purwandhono, Azham; Bumi, Candra
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
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Abstract

The obesityincreases the riskof morbidityandmortalityof type2diabetesandcardiovasculardisease. Leptin, an adipocytokine that regulate metabolic process, was increase in obesity. Hyperleptinemia activate the transcriptionfactorNuclearFactorKappaB(NF-kB), leading to the occurrence of inflammation. The chronic inflammation in vessels would cause the dysfunction of endothelial cell.Bitter melon (Momordica charantia) has beenused by many peoplefor the treatmentof diseases. Studies showthatbitter melon fruitcontainssaponins(charantins) andalkaloids. This study aimed toinvestigate the effect of bitter melon extract on the level of activated NF-κB. Cultured endothelial cell, HUVECs (Human Umbilical Vein Endothelial), is used for a model of endothelial cells. There are 5 groups: 1) HUVECs culture without leptin as negative control; 2) HUVECs culture with leptin as positive control; and 3 groups given various dosages of bitter melon (0.1 mg/ml; 0.05 mg/ml; 0.01 mg/ml; 0.005 mg/ml; 0.001 mg/ml) and Leptin. Leptin (500 ng/ml) is added on HUVECs to stimulate responds of endothelial cells mimicking obesity condition. Momordica charantiawere given 2 h before leptin treatment. The level of activated NF-KB was measured by ELISA. This study showed that leptin significantly induce NFkB activation and the treatment of bitter melon inhibit NFkB activation significantly (p<0.05). Keywords: Leptin, Endothel, bitter melon, NFkB
The Making of Salty Soy Sauce From Koro Benguk (Mucuna pruriens) (Study of Saline Concentration of Salt Solution and Duration of Maromis Fermentation) Mulyadi, Arie Febrianto; ., Wignyanto; Hidayah, Eka Yaniar Putri Nur
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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The objectives of this study were to determine the saline concentration and moromi’s fermentation duration of koro benguk salty soy sauce at best organolepticly and determine consumers’ preferences towards koro benguk salty soy sauce from the best treatment results. The study was conducted using a randomized design method using two factors: the saline concentration (17%; 20%; and 23%) and duration of moromi’s fermentation (2; 3; and 4 weeks). The best treatment results based on the Friedman test was on the saline concentration of 17% and moromi’s fermentation duration was 4 weeks, with the NP value of 1,000; had a preference color level of 5:40 (liked); aroma of 4.30 (rather liked); flavor of 4.55 (rather liked); and viscosityof 5.05 (liked). The obtained protein was  7.14%; and dissolved solids of 27 brix. Consumers’ preferences towards the best treatment showed that product of koro benguk salty soy sauce was acceptable to consumers. Keywords: Moromi’s Fermentation, Soy Sauce, Saline Concentration, Koro Benguk
The Effect Of Cholecalciferol On Fasting Blood Glucose In Streptozotocin-induced Hyperglycemia Mice Sakinah, Elly Nurus
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Glucose uptake into skeletal muscle cells require insulin-dependent and insulin independent signaling pathways, both leading to the translocation of glucose transporter-4 (GLUT4) to the plasma membrane. Insulin resistance occurs due to failure of insulin signaling to translocate GLUT4 resulting in the failure of glucose uptake and causing hyperglycemia. Cholecalciferol is known to have a function in regulating calcium homeostasis was shown to increase the synthesis of insulin and increasing insulin sensitivity. The purpose of this study is to explain the role of cholecalciferol to decreased fasting blood glucose in streptozotocin-induced hyperglyemia mice. 30 mice adapted for one week and then induced using 150mg/kgBW streptozotocin (STZ) intraperitoneally, After experiencing hyperglycemia mice were divided into 5 groups (n=6 each), Group I (hyperglycemic control), group II (25ng cholecalciferol), group III (50ng cholecalciferol), group IV (100ng cholecalciferol), and group V (metformin 300mg/kgBBB). Cholecalciferol given orally for 14th days. On day 15th the examination of fasting blood sugar levels were taken and the mice. Fasting blood sugar levels measured using a glucometer. Based on statistical analysis showed that there were significant differences in fasting blood sugar levels between treatment groups (p<0,001). Based on univariate regression analyses there was negative correlation of cholecalciferol with fasting blood glucose (p<0,001). Cholecalciferol may lower fasting blood sugar levels in hyperglycemia mice models. Keywords: cholecalciferol, fasting blood glucose, STZ.
Lead Distribution on Marsilea crenata and Its Effect on Growth and Total Protein Content Nurhayati, Arry Y.; Hariadi, Yuda C
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

The increasing risk of lead poisoning such as neuro-behavioral disorders and neurological damage; hypertension, brain and kidney damage; DNA and antioxidant defense system cell damage in developing countries due to the increase in lead contaminated environment. This research was aimed to investigate the effects of prolonged lead on protein levels in the Marsilea crenata plant. The plants have been used for generation by local farmers as a daily source of vegetables during crop growing season. The plants are also known as an exotic cuisine known as Semanggi Surabaya. Marsilea crenata were grown inside a glasshouse using a modified Hoagland solution in different levels of Pb. The growth, Pb content, and total protein content were then measured for eight weeks during exposure. The result has shown that the root of Marsilea crenata has the capability to absorb lead and translocate them inside its shoots and leaves, with the biggest amount were translocated to the younger leaves during one week of exposure and was then accumulated in the old leaves after longer exposures. Total protein content was higher on younger leaves than in older leaves, followed by shoot and then the root. The result has shown that with deep knowledge of Marsilea crenata it is possible of using it as a phytoremediator or as a source of food since the total protein was translocated on the edible part. Still, awareness is still needed since the plant may contain high levels of Pb with the existence of stress symptoms. It should be avoided to reduce the risk of lead poisoning. Keywords: Lead, Marsilea crenata, growth, protein
Isolation and Physicochemical Properties of Rice Bran Protein from Heat-Stabilized Rice Bran Kusumawaty, Inneke; Fardiaz, Dedi; Andarwulan, Nuri; Widowati, Sri; Budijanto, Slamet
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Isolation and the physicochemical properties of rice bran protein concentrate (RBPC) produced from unstabilized and heat-stabilized Pandanwangi and Ciherang rice bran were studied. Rice bran stabilization process optimization done on previous research resulted in the extrusion conditions at a temperature of 130.96oC and screw speed 26.65 Hz. Kjeldahl analysis showed that protein content of unstabilized rice bran protein concentrates (URBPC) and stabilized rice bran protein concentrates (SRBPC) of Pandanwangi and Ciherang were 60.76%, 61.38%, 60.19%, and 60.23% respectively. Amino acid composition showed that polar amino acid composition of RBPC Ciherang was higher than that in Pandanwangi leading to its solubility. The protein percentage of acid-soluble glutelin of Pandanwangi was higher than that in Ciherang rice bran protein concentrate. The molecular weight were in range from 11.19 to 60.29 kDa. Glutelin differentiated into α-glutelin (30-39 kDa) and β-glutelin (19-25 kDa). The  RBPCs from two varieties had similar denaturation temperatures (77.22 - 77.99oC) with enthalpy ranged between 109.72 J/g and 200.98 J/g. Foaming stability and emulsion activities had similiar pattern with solubilities profile and showed no significant difference between varieties (p> 0.05). This finding shows potential protein concentrate of  both heat-stabilized and unstabilized rice bran as food ingredient. Keywords: Heat-stabilized rice bran, rice bran protein, physicochemical properties

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