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UNEJ e-Proceeding
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Articles 1,005 Documents
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Mineral Nitrogen in Soil of Sugarcane Plantation of PG Jatiroto Wijaya, Ketut Anom
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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The objective of research was to study mineral N content in soil of PG Jatiroto sugarcane plantation, Lumajang, East Jawa. Mineral N of soil is very important data in utilizing the precise N fertilization method. Rate based fertilization had been discontinuedly utilized by some advanced countries since 1980s because it was considered to be environmentally unfriendly, not appropriate for precision agriculture which oriented toward inner quality.  In the future, Indonesia is also expected to implement the method of accurate N supply on sugarcane to replace the rate based method. Nitrate and ammonium content in soil analized using Cataldo method and  Conway Dish method. Soil samples were taken on two kind of soil texture (sandy soil and loamy soil). Result: content of mineral nitrogen in soil of sugarcane plantation was unexpected extrimly high (2.000-10.000 kg/ha) compare to nitrogen need of sugarcane for yielding highest sugar content (around 350 kg N/ha), nitrate-Nitrogen in general, was very much lower (20-150 kg/ha) than ammonium-Nitrogen (2.500-9.800 kg/ha). Conclusion: mineral N in soil of sugarcane plantation of PG Jatiroto extrimly high and much higher than need of N of sugarcane to produce high sugar content, ammonium-N was the majority of mineral N form in both of soil types.. Keywords: sugar plantation, soil, nitrogen-mineral, nitrate, ammonium
Physical Properties of Gel and Edible Plastic from Whey and Tapioca In Various Ratio and pH Value Lindriati, Triana; ., Herlina; Nafi, Ahmad
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Edible plastic usually made from hydrocolloids and could be produced by solvent casting method whereas gel was made before casting. Potential of whey protein combined with tapioca as edible plastic based component was studied at this research. pH of solvent could affect interaction of whey and tapioca during  gel preparation. The gel’s characters affected on plastic’s characters . The research objectives were to study effect of whey and tapioca ratio and pH of solvent on physical properties of gel and edible plastics. Randomized Factorial Block Design were used. The two factors  were  porpotion of whey protein-starch mixture (0%, 20%, 40%, 60%, 80%, 100%) and pH of solvent ( 4, 7 and 9). Increasing of whey porpotion could increase solubility of gel but decrease gel’s lightness and texture. Increasing of pH could increase gel’s solubility and decrease lightness and texture. There was significant effect (p≤0,05) of whey porpotion and pH interaction on gel’s characters. Increasing of whey porpotion could increase tensile strength and decrease elongation and solubility of edible plastics but the increasing of tensile strength was not significant (p≥0,05). Increasing of pH could increase elongation and decrease tensile strength and plastic’s solubility. There wasn’t significant effect (p≥0,05) of whey porpotion and pH interaction on edible plastic’s characters. The result showed that whey addition had decreased plastic’s characters even at 100% whey ratio tensile strength and elongation value were zero, edible plastic could not be produced from 100% whey. Keywords: texture, solubility, elongation, tensile strength, interaction
Specific sequence of Plasmodium falciparum DBL domains associated with severe malaria outcome Sulistyaningsih, Erma; Fitri, Loeki Enggar; Loescher, Thomas; Berens-Riha, Nicole
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Duffy-binding like (DBL) domains of Plasmodium falciparum are believed to be involved in erythrocytes invasion and infected erythrocytes cytoadhesion during the blood stage of malaria infection. In Plasmodium falciparum, DBL domains found in the two different protein families; Erythrocyte Binding Ligand (EBL) including EBA-175, EBA-140, EBA-181 and EBL-1, and Plasmodium falciparum Erythrocyte Membrane Protein-1. The study aimed at investigating the specific sequence of Plasmodium falciparum involved in severe malaria outcome.Blood samples from severe and uncomplicated falciparum malaria cases from Papua and South Kalimantan province, Indonesia, were collected for DNA extraction. A dried blood on filter paper were used for RNA extraction. PCR was performed using UNIEBP primers and directly sequenced. Internal var D primers were designed according to the sequencing of the ~550 bp band produced by UNIEBP primers. The nucleotide sequences were analyzed by NCBI BLAST. Multiple bands ranging from nearly 250 bp to 1 kb were resulted from gDNA in all samples. Two isolates yielded bands of 450 and 525 bp, three isolates showed three bands additionally 250 bp, one isolate presented four bands additionally 800 bp and one isolate resulted one band additionally 1 kb.Amplification of cDNA from severe malaria cases produced one to four bands ranging from 250 bp to 700 bp, and no band observed from cDNA of uncomplicated malaria. Sequencing of the 418 bp bands matched with the eba-175 gene, the 316 bp determined as DBL1a domain and 486 bp band matched with the DBLg domain isolated from placenta of PAM’s Malawian woman. The expression of a 237 bp sequence corresponding to var D gene, was detected solely in severe malaria patients, implicating an association of gene expression and manifestation of severe malaria. Further characterization of the var D gene with a larger sample size is required to draw a definite conclusion. Keywords: DBL domains, Plasmodium falciparum, severe malaria, var D gene.
Zinc Biofortification of Rice Using Fish Protein Hydrolysates Mixed With Zinc Sulfate. Sjaifullah, Achmad
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Zinc deficiency is one of the serious health problems experienced by half of the world population who consumes rice as a staple food. Zn biofortification of rice is aimed to increase the concentration of Zn, and its bioavailability in rice is one of a growing research. Some of the compounds used in Zn biofortification include Zn sulfate, Zn EDTA, Zn nitrate, Zn chloride, Zn organic and Zn amino acids (i.e., a combination of ZnSO4 and amino acids). Among those various compounds of zincs, Zn biofortification of rice by foliar application of solution ZnSO4 mixed with amino acid is the best in improving the concentration of Zn in polished rice and also its bio-availability. This article discusses the effect of the use of fish protein hydrolysate solution containing amino acids and peptides added with ZnSO4 for zinc biofortification of rice. Fish hydrolysate solution mixed with ZnSO4 were applied by foliar spraying on rice in three applications at intervals of 8 days, starting 73 days after planting, when the rice begins to flower. Spraying was performed in the morning, between 08:00 - 09:30 o’clock on the samples and the control plots. The results showed that the rice crop sprayed with fish hydrolysate mixed with ZnSO4 has increased the rice production by 7 % in weight and the polished rice which was sprayed with fish hydrolysate mixed with ZnSO4 contains 40 % higher of Zn (17.1 ppm) than in controls (12.0 ppm). Keywords: zinc biofortification, fish protein hydrolysate, zinc sulfates.
The Potency of Protein Extracts from Candida albicansas Bioreceptor on Immunosensor for Diagnosis of Candidiasis ., Masfufatun; Kumala, Noer; Baktir, Afaf
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Currently diagnosis of candidiasis still usingthe traditional standard blood culture method. The traditional method were less sensitive andtime consuming. The purpose of this research were to develope the more sensitive immunosensor based method, and to examine the potency of C. albicans protein extract as bioreceptor to  detect  C. albicans and its biofilm in the blood of candidiasis patients.The research methods include: (1) preparation of  digestive gland liquid of snail (Achatina fulica); (2) extraction of protein from C. albicans  through  enzimatis and mechanic methodsand (3) analyzing the protein extract as bioreseptor through immunodot assay.The research results showed that the snail enzymes has protein content 1.35 mg/ml and  specific activity 1.96 unit/mg. The snail enzyme hydrolyzed  the cell wall of C. albicans  with and without sonication, producedplanktonic extracell protein extract (PEP) and biofilmextracell proteinextract (PEB), planktonic intracell protein extract (PIP), and biofilmintracell protein extract (PIB), withprotein content 1.44; 1.29; 1.29 and 1.21 mg/ml respectively. Thebiofilm intracell protein (BIP) showed antigenic property towardantibody anti-Candida (positive control),giving red spot on imunodot assay. Immunodot assay can distinguishnegative control serum (health man) and positive Candidiasis control by using antigen 1 mg/ml and50ml serum. Keywords: C. albicans, candidiasis, biofilm, immunodot assay
Exploration of Lipase Enzyme from Soil through Metagenomic Approach Sumarsih, Sri; Baktir, Afaf; Andina, Budi Putri Ayu
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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This research aimed to explore lipase enzyme from soil through metagenomic approach. The DNA was extracted directly from the topsoil and river sediment by SDS-based lysis method. The soil genomic DNA was sheared into approximately 40-kb fragments The construction of genomic library was carried out by cloning the fragment DNA into  fosmid pCC2FOS vector and host E. coli EPI300-T1R accordance with the protocols of CopyControl™ Fosmid production library kit. Screening of lipase-producing clones was performed based on its lipolytic activity using Luria-Bertani agar plate 12.5 µg/ ml chloramphenicol, 10 µg/ml Rhodamine B and 1 % olive oil as a substrate. The assay plates were incubated at 550 C. The lipolytic activity was identified as an orange around colonies. There were 5 (five) clones of 32 screened-clones that expressed lipolytic activity. The next research will characterize the enzyme and determine the sequence of the enzyme-encoding gene. Keywords: Lipase, soil, metagenomic, fosmid vector, pCC2FOS, E. coli EPI300-T1R
Optimization pH of Enzymatic Hydrolysis of Endo-1,4-β-Xylanase for Xylooligosaccharides Production Ratnadewi, Anak Agung Istri; Kurniawan, Andika Ade; Handayani, Wuryanti
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Xylooligosaccharides (XOS) with polymerisation degree between 2 till 10 monomer  have prebiotic effect for better digestion system. In this research, production of XOS was performed by enzymatic hydrolysis of xylan with endo-1,4-β-xylanases enzyme. Endo 1,4-β-xylanases enzyme was aqcuired from Bacillus sp. isolated from termite’s abdominal.Meanwhile oat xylan was used as substrate. Optimal condition of enzymatic hydrolysis was evaluated at pH: 4, 5, 6, and 7 with incubation time from 5 to 20 h.pH 5 was optimum pH to produce XOS from 0.8 % xylan oat at 40 oC.The hydrolysis product purified and analyzed by thin layer chromatographyyielding spot of xylobiose, xylotriose, xilotetraose and xilopentaose. Further analysis by HPLC indicated dominant xilopentaosa X5 (3522 ppm) among  the other XOS   X2 (14 ppm), X3 (43 ppm) and X4 (15 ppm). Keywords: Xylooligosaccharides, endo-1,4-β- endoxylanase
Transformation of Plasmid pET Endo-1,4-β-xilanase from E. coli TOP10 to E. coli BL21 Santoso, Agung Budi; Kurniawati, Eka Yuni; Ratnadewi, Anak Agung Istri
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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In this research we successfully transmited Plasmid pET- Endo from E.coli TOP10 to E.coli BL21. Plasmid pET Endo is recombinant plasmid base on pET-30a(+)  inserted with gene of endo-1,4-β-xilanase isolated from bacillus subtilis sp which is originally living in termite abdomen. First step is isolation of plasmid pET-Endo from E.coli TOP10 with alkaline lyses. Denaturation and renaturation of DNA occurred then separated with centrifugation. Plasmid pET-Endo is smaller than Chromosome DNA. Agarose gel electrophoresis confirmed that Plasmid pET-Endo has isolated. Electrogram of pET-Endo show the band in 6022 bp compare to empty plasmid pET-30a(+) 5422 bp. E.Coli BL21 got pretreatment with  CaCl2 solution to make cell competent for transformation. Isolated pET-Endo inserted to E.coli BL21 with heat shock method. Resistance test with antibiotic has done to know the result of transformation. E.coli BL21 contained plasmid pET-Endo will survive in kanamycin agar media. Colony of E.coli BL21 then cultured in liquid media and examinee it’s growth phase. IPTG as gene inducer given after 2,5 hour of inoculation. Crude enzyme tested for xilanase activity and well proved. Isolation of plasmid pET-Endo then running in Agarose gel electrophoresis also confirm good result of transformation.
The Influence of Supplementary Feeding (Probiotic and Azolla pinnata) on Protein and Amino Acids Content in Patin Fish Oktavianawati, Ika; Hermiastuti, Meirinda; Rahmawati, Novita; Handayani, Wuriyanti; Winata, I Nyoman Adi
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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One of the important factors in fish farming is feeding. Feed intake is able to affect the growth and production of fish, especially on nutritional content such as protein. In this research, Patin (Pangasius djambal), a catfish, were given variations in feed, i.e pellets only (P1) as a control; pellets and probiotic (P2); pellets and Azolla pinnata (P3). A. piñata (known locally as matalele) is a water plant that has complete essential amino acids including threonine, valine, methionine, isoleucine, leucine, phenylalanine, lysine, histidine, arginine and tryptophan. Probiotic is living microbial cells that affects the speed of feed fermentation and helps the absorption of food in the gastro intestinal tract. The results show that P2 gains more weight than P1 and P3. This feed variations also give different levels of crude protein in fish meat P1, P2, and P3 that are in the order 14.62%; 15.74%; and 13:35%. Based on the results of LC-MS analysis, the amount and the dominant amino acids content in the protein of P1, P2, and P3 are 13 amino acids–aspartic acid; 13 aminoacids–glutamic acid; and 14 amino acids and is predominantly asparagine. Keywords: protein, amino acid, patin, probiotic, Azolla pinnata
Screening and Isolation of Cellulolytic Bacteria From Bagasse and Characterization of The Cellullase Produced Hartanti, Lanny; Susanto, Fandy; Utami, Caesilia Putri; Sukarti, Emi; Setiawan, Henry Kurnia; Ervina, Martha
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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The research of screening and isolation of cellulase-producing bacteria from bagasse had been done. The objectives of the research were to obtain cellulolytic bacteria isolate, to determine the genus of the isolates and also to characterize the crude enzymes produced. One isolate with the highest activity was chosen to be purified further bystreak plate and pour plate methods in carboxyl methyl cellulose(CMC)  agar media. The genus of the pure isolated bacteria was further characterized by macroscopic and microscopic properties, and biochemical assays using conventional methods and MicrobactTM Kit: gram-negative identification system (Oxoid). The result showed that the isolated cellulolytic bacteria had a close character similarity to Bacillus pumilus. The isolate was grown on MHB + CMC media with 2 hours of adaptation phase followed by logarithmic phase until 21 hours of incubation. Cellulase was started to be produced after 4 hours of fermentation and reached the highest activity at 21 hours of fermentation. The cellulase produced had the pH optimum at pH 5 and a temperature optimum at 60 °C, with optimum activity ranging from 0.0231 to 0.0264 U /ml. One unit of cellulase activity is defined as the amount of enzyme that will catalyze the production of 1 mg of glucose per minute at 37°C and pH 7.0. Keywords:  bagasse, bacteria isolation, cellulolytic, biochemical characterization, enzyme characterization

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