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BIOCOMPABILITY TEST OF HARUAN FISH (Channa striata) BONE HYDROXYAPATITE TO FIBROBLAST CELL AS PERIODONTAL POCKET THERAPY (In Vitro Study on BHK-21 Fibroblast Cell with Hydroxyapatite of Haruan Fish Bone (Channa striata) as Bone Graft Material) Maulidah Maulidah; Irnamanda DH; Fransiska Uli Arta Panjaitan
Dentino : Jurnal Kedokteran Gigi Vol 3, No 2 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i2.5370

Background: Periodontitis is an inflammation of the soft tissues and hard tissues that support the tooth characterized by periodontal pocket formation, recession to resorption of alveolar bone. So far, alveolar bone resorption caused by periodontitis can be treated with bone graft therapy. Xenograft is a type of bone graft that has many advantages such as can be obtained from natural materials, low in price and has minimal virus transmission. Hydroxyapatite of haruan fish bone is a type of xenograft material that has never been applied in medical field. Objective: This study aims to analyze the biocompatibility of haruan fish bone hydroxyapatite (Channa striata) against BHK-21 fibroblast cells via viability. Method: This was a laboratory experimental study with posttest only control group design, using MTT assay method and BHK-21 fibroblast cell viability was calculated using optical density formula. Results: The concentrations of 0,2109 mg/ml, 0,4218 mg/ml 0,8437 mg/ml, 1,6875 mg/ml, 3,3375 mg/ml and 6,75 mg/ml were biocompatible against fibroblast cells BHK-21 whereas at concentrations of 54 mg/ml, 27 mg/ml and 13,5 mg/ml were toxic to BHK-21 fibroblast cells. One Way Anova test and Bonferroni test showed concentrations of 54 mg/ml, 27 mg/ml and 13,5 mg/ml had significant differences to other concentrations. Conclusion: Hydroxyapatite of haruan fish bone (Channa striata) at some concentration is biocompatible against BHK-21 fibroblast cells.

KEKERASAN PERMUKAAN RESIN-MODIFIED GLASS IONOMER CEMENT SETELAH PERENDAMAN DALAM AIR SUNGAI (Penelitian Menggunakan Air Sungai Desa Anjir Pasar, Barito Kuala, Kalimantan Selatan) Selvia Shella; Muhammad Yanuar Ichrom Nahzi; Isyana Erlita
Dentino : Jurnal Kedokteran Gigi Vol 2, No 1 (2017)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v2i1.2602

ABSTRACTBackground: Resin-modified glass ionomer cement is a hybrid form of glass ionomer cement and resin. Added resin (HEMA) in this material allows its characteristics to improve, especially its resistance of acid. The water of Desa Anjir Pasar River has acidic nature with pH as low as 3-5. Purpose: The aim of this study is to find surface hardness difference of resin modified glass ionomer cement after it was soaked in the water of Anjir Pasar Village River compared to aquadest-soaked materials. Method: The study used resin-modified-glass ionomer cement specimens prepared in cylindrical-shaped with diameter of 10 mm and thickness of 2 mm. One group was soaked in aquadest (control) and another group in river water with pH of 3 for 4.5 days (equivalent to 3 years of exposure) before measuring the surface hardness of each group. Data was analyzed using parametric analysis Independent T-Test 95% (α = 0.05) which presented p value = 0.003 (p<0.05). Result: The result indicated that there was a significant difference of surface hardness between resin modified glass ionomer cement soaked in the water of Anjir Pasar Village River with pH of 3 for 4.5 days and in the aquadest. Conclusion: Based in this study there is a great differences of hardness on resin-modified glass ionomer cement materials that are immersed in river water to sterilize aquades.Keywords: Resin-Modified Glass Ionomer Cement, river water, surface hardness

EFFECTIVENESS OF KARAMUNTING FLOWER EXTRACT (Melastoma malabathricum L) ON THE STREPTOCOCCUS MUTANS GLUCOSYLTRANSFERASE ENZYME ACTIVITY Muhammad Zainal Fikri; Deby Kania Tri Putri; Muhammad Yanuar Ichrom Nahzi
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7046

Background: Caries is a chronic disease of mineral ions continuous loss caused by bacteria in oral cavity. One of the main bacteria is Streptococcus mutans. This bacterium is able to synthesize the glucosyltransferase enzyme (GTF), which function is to catalyze polysaccharides in the form of sucrose into sticky glucans. This pathogenic property also has function in helping bacteria to stick and accumulate on the tooth surface, so it can increase the occurrence of the demineralization process and dental caries. Karamunting flower extract, as a natural ingredient, has antibacterial property. This property is caused by the content of phenol, flavonoid and tannin, which can reduce the GTF S. mutans enzyme activity. Objectives: This study aimed to analyze the effectiveness of karamunting flower extract (Melastoma malabathricum L) on the activity of the Stretococcus mutans glucosyltransferase enzyme. Methods: This study used a post-test only with control group design with karamunting flower extract samples in various concentration, including 40%, 50%, 60%, 70%, measuring enzyme activity with 4 repetitions in each group. Results: The data were analyzed using One Way ANOVA, which got p = 0,000 (p <0.05) and followed by Post Hoc LSD test that showed significant difference between each group (p <0.05). Data showed that concentrations of 40%, 50%, 60%, and 70% can reduce GTF S. mutans enzyme activity. Conclusion: Karamunting flower extract has the effectiveness of reducing the GTF enzyme activity of S. mutans.

THE EFFECTIVENESS OF DAYAK ONION BULBS EXTRACT (ELEUTHERINE PALMIFOLIA (L) MERR.) AGAINST ROOT CANAL MIXED BACTERIAL (Preface Study As Root Canal Irrigation Materials) Ratih Yusnita; Muhammad Yanuar Ichrom Nahzi; Sherli Diana
Dentino : Jurnal Kedokteran Gigi Vol 3, No 2 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i2.5367

Background: Dayak onion (Eleutherine palmifolia (L) Merr) is a native plant from Central Kalimantan and has been trusted as a multifunctional plant, one of them is as an antibacterial. Purpose: To find out the difference in the effectiveness of Dayak onion bulbs extract with various concentrations on the growth of mixed bacterial of root canal to be used as an alternative root canal irrigation. Methods: This study used true experimental research design namely post-test only with control group design treated with 5 different treatments, Dayak onion bulbs extract at concentrations of 20 mg/ml, 40 mg/ml, 60 mg/ml, 80 mg/ml and 5,25% sodium hypochlorite, and done with 5 times repetitions. The antibacterial effect test was performed using diffusion method. Result: The result of average diameter of inhibition zone formed on Dayak onion bulbs extract at concentrations of 20 mg / ml, 40 mg / ml, 60 mg / ml, 80 mg / ml and 5.25% sodium hypochlorite to the root canal mixed bacterial in sequence i.e. 12.74 mm , 15.82 mm, 20.38 mm, 25.90 mm and 23.52 mm. One Way Anova Test result and Post Hoc LSD test obtained the value p=0,000 (p<0,05). It proved that there were difference of antibacterial activity of Dayak onion bulbs extract concentration 20 mg/ml, 40 mg/ml, 60 mg/ml, 80 mg/ml and sodium hypochlorite 5,25% against mixed bacteria of the root canal. Conclusion: Dayak onion bulbs extract with concentration of 80 mg/ml has greatest antibacterial activity to inhibit the growth of root canal mixed bacterial.

COMPARISON OF COLOR CHANGE IN GLASS IONOMER CEMENT (GIC) AFTER TOPICAL FLUORIDE APPLICATION Using Type II Conventional GIC and Topical Fluoride in Sodium Fluoride (NaF) and Acidulated Phosphate Fluoride (APF) Preparations Muhammad Yanuar Ichrom Nahzi; Isyana Erlita; A.M. Dewi Fathiya Julianti
Dentino : Jurnal Kedokteran Gigi Vol 2, No 1 (2017)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v2i1.2590

ABSTRACKBackground : Conventional glass ionomer cement is an applicative material used in dentistry. Topical flouride is widely used as a preventive measure against caries; it is available in various forms, such as Sodium Flouride (NaF), Stannous Flouride (SnF2), and Acidulated Phosphate Flouride (APF). There are a few considerations in selecting which form to use, and one of them is the physical characteristic which may interact and change the color of previously placed conventional glass ionomer cement. Purpose : This study aimed to find out whether there was a difference of color change in conventional glass ionomer cement after topical fluoride application between NaF and APF form. Method : This study was a true experimental research using pre and posttest with control group design. There were 18 samples divided into 2 treatment groups and 1 control group. Each group had 6 samples. The measurement was carried out using optical spectrometer (OPT 101 type of photo detector) and microvolt digital. Result: The mean color change in conventional glass ionomer cement after APF and NaF topical application was 1,7300 mv and 0,4983 mv respectively. One way ANOVA test and post hoc LSD test results showed a significant difference (p<0.05). Conclusion : Discoloration on APF topical application was higher than NaF.Keywords : Acidulated Phosphate Fluoride (APF), discoloration of conventional glass ionomer cement, Sodium Fluoride (NaF), topical fluoride.

PENGARUH ASAP ROKOK TERHADAP PERUBAHAN WARNA PADA BASIS GIGI TIRUAN RESIN TERMOPLASTIK NILON (Penelitian pada 2 Jenis resin termoplastik nilon berdasarkan suhu pembuatan 287,7° C dan 302° C) Riezki Amalia Rahmah; Debby Saputera; Dewi Puspitasari
Dentino : Jurnal Kedokteran Gigi Vol 2, No 1 (2017)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v2i1.2606

ABSTRACTBackground:Loss of teeth could impact on the mastication efficiency. To overcome this problem, the patient can wore denture or prosthesis. One of thematerials to made denture base is Valplast® and Lucitone® FRS™. Denture base material was likely to absorbed various of contaminants such as tar that were contained in cigarettes. It became a factor of discoloration on denture base. Purpose: To determine whether there was a color change of the nylon thermoplastic resin after exposed to cigarette smoke. Methods:This type of research was true experimental with post-test only and control design, the number of samples that used were 24 pieces consisting of four groups, two of them were Valplast®, one group exposed to smoke and others were not exposed to smoke and the other two groups were Lucitone® FRS™, one group were exposed to smoke and others were not exposed to smoke. Result:There were significant differences between the group of Valplast® that exposed to smoke with the group of Valplast® that not exposed to smoke of 0.000 (p<0.05) and between the group of Lucitone® FRS™ that exposed to smoke with the group of Lucitone® FRS™ that not exposed to smoke of 0.000 (p<0.05) as well as between the group of Valplast® that exposed to smoke with the group of Lucitone® FRS™ that exposed to smoke of 0.000 (p<0.05).Conclusion: There were changed of color in the nylon thermoplastic resin after exposed to cigarette smoke.Keywords: Nylon thermoplastic resin, Discoloration, Cigarette smoke.

TOXICITY TEST OF DAYAK ONION BULBS EXTRACT (Eleuthherine palmifolia (L) Merr) ON Artemia salina LEACH USING BSLT METHOD (Preface Study As Root Canal Irrigation Materials) Khairunnisa Khairunnisa; Muhammad Yanuar Ichrom Nahzi; Sherli Diana
Dentino : Jurnal Kedokteran Gigi Vol 3, No 1 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i1.4609

Background: Dayak onion (Eleutherine palmifolia (L) Merr) is a native plant from Central Kalimantan, which contains active compounds as anti-bacterials that can be used as an alternative material for root canal irrigation. High toxicity is one of the causes of root canal failure, so it takes a toxicity test to determine the toxic effects of the dayak onion bulbs extract. Purpose: To analyze the difference of toxic effects with various concentrations of dayak onion bulbs extract on Artemia salina Leach using BSLT method. Material and Methods: This study used true experimental research design, namely post-test only with control group design treated with 11 different treatments, which were dayak onion bulbs extract with concentrations 10 mg/ml, 20 mg/ml, 30 mg/ml, 40 mg/ml, 50 mg/ml, 60 mg/ml, 70 mg/ml, 80 mg/ml, 90 mg/ml, 100 mg/ml and sea water as its negative control, and done with 3 times repetitions. Result: The result of the probit analysis in the amount of LC50 was 70,371 mg/ml. The data analysis used Shapiro-Wilk and Levene’s Test results p > 0,05 means that all the data was normally distributed and homogenous. Further test with One Way Anova obtained result p < 0,05 which means there was a significant difference in the number of dead larvae between concentrations. Further test with Pos-hoc LSD obtained result that there was a significant difference at concentration 100 mg/ml with 10 mg/ml with value p=0,001. Conclusion: The highest toxic effect on dayak onion bulbs extract on Artemia salina Leach is at concentration of 100 mg/ml.

HUBUNGAN KEBIASAAN MEROKOK DENGAN PEMBENTUKAN STAIN (NODA GIGI) PADA PASIEN DI POLI GIGI RSUD RATU ZALECHA MARTAPURA Enny Khalisa; Rosihan Adhani; Syamsul Arifin
Dentino : Jurnal Kedokteran Gigi Vol 1, No 1 (2016)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v1i1.416

ABSTRACT Background: Stain (teeth stain) is pigmented deposit on teeth surface. Stain is an esthetic problem for some people. Stain affected by some factors, one of them is smoking. Purpose: The purpose of this research is to identify the relation between smoking habit (smoking duration, cigarettes type, and cigarettes smoked per day) with formation of stain. Methods: Type of this research was observational analytic with cross sectional design. The method used was interview about smoking duration, cigarettes type, number of cigarettes smoked per day and clinical examination to look the presence of stain in teeth surface on 30 male smokers patients in dental clinic RSUD Ratu Zalecha Martapura. Results: The results presented 23 respondents (76,7%) from 30 respondents had stain formation. There was no relation between smoking duration with stain formation where p value on statistic test of kolmogorov-smirnov was 0,992 (>0,05). There was no relation between cigarettes type with stain formation where p value on statistic test of fisher was 0,071 (p>0,05). There was no relation between number of cigarettes smoked per day with stain formation where p value on statistics test of kolmogorov-smirnov was 0,652 (p>0,05). ABSTRAK Latar Belakang: Stain (noda gigi) adalah deposit berpigmen pada permukaan gigi. Stain merupakan masalah estetik bagi sebagian orang. Stain dipengaruhi oleh banyak faktor, salah satunya adalah merokok. Tujuan: Tujuan penelitian adalah mengetahui hubungan antara kebiasaan merokok (lama merokok, jenis rokok, dan rokok yang dihisap per hari) dengan pembentukan stain. Metode: Jenis penelitian ini menggunakan rancangan analitik observasional dengan desain cross sectional. Metode yang dilakukan adalah wawancara mengenai lama merokok, jenis rokok, dan jumlah rokok yang hisap per hari serta pemeriksaan klinis untuk melihat ada atau tidak pembentukan stain di permukaan gigi pada 30 pasien laki-laki perokok di poli gigi RSUD Ratu Zalecha Martapura. Hasil: Hasil penelitian yang diperoleh yaitu terdapat 23 responden (76,7%) yang memiliki pembentukan stain dari 30 responden. Tidak terdapat hubungan antara lama merokok dengan pembentukan stain, dimana nilai p pada uji statistik dengan mengunakan kolmogorov-smirnov adalah 0,992 (p>0,05). Tidak terdapat hubungan antara jenis rokok dengan pembentukan stain, dimana nilai p pada uji statstik dengan mengunakan fisher adalah 0,071 (p>0,05). Tidak terdapat hubungan antara jumlah rokok yang dihisap per hari dengan pembentukan stain, dimana nilai p pada uji statstik dengan mengunakan kolmogorovsmirnov adalah 0,653 (p>0,05).

UJI EFEKTIVITAS ANTIBAKTERI SEDIAAN TUNGGAL DIBANDINGKAN KOMBINASI SEDUHAN DAUN TEH HIJAU (Camellia sinensis) DAN MADU (Studi in Vitro terhadap Jumlah Koloni Bakteri Rongga Mulut) Tinjauan pada Mahasiswa PSKG FK Unlam Banjarmasin Angkatan 2011-2013 Wahyuni A; Nurdiana Dewi; Lia Yulia Budiarti
Dentino : Jurnal Kedokteran Gigi Vol 1, No 2 (2016)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v1i2.552

ABSTRACTGreen tea has various active substances; one of them is polyphenol, mostly cathechin, an active compound which can protect teeth from caries because of its anti-streptococcal activity. A high content of minerals in honey has alkali characteristic thus the disinfectant trait in oral cavity. The aim of this study was to assess whether single and combined preparations of brewed green tea and honey can decrease bacterial colony count in oral cavity. This study was quasi experimental with pretest and posttest controlled group design. Samples of 38 Students of Dentistry Study Program Universitas lambung Mangkurat were divided into 19 groups: 3 groups were given 25%, 50%, 100% green tea in single preparations, 4 groups were given 6,25%, 12,5%, 25%, 50% honey in single preparations, and 12 groups were given combined preparations of green tea and honey. Bacterial colony before and after gargling grown on isolated medium was counted using colony counter. Repeated anova test showed p value of 0,037 (p < 0,05), stating that there was a significant difference among treatment groups. In conclusion, combined preparations of 50% green tea + 12,5% honey and 100% green tea + 25% honey were more effective in decreasing bacterial colony count. ABSTRAKTeh hijau mempunyai beberapa komponen aktif yang salah satunya adalah polifenol berupa katekin, suatu senyawa aktif dalam melindungi gigi dari karies karena memiliki aktivitas anti-streptococcal. Kandungan mineral yang tinggi pada madu mempunyai sifat basa (mengandung unsur alkali) sehingga dapat berfungsi sebagai desinfektan terhadap rongga mulut. Penelitian ini bertujuan untuk mengetahui apakah sediaan tunggal dan kombinasi seduhan daun teh hijau dan madu dapat menurunkan jumlah koloni bakteri rongga mulut. Penelitian ini bersifat eksperimental kuasi dengan rancangan pretest and posttest controlled group design. Sampel sebanyak 38 orang mahasiswa Program Studi Kedokteran Gigi Fakultas Kedokteran yang dibagi dalam 19 kelompok yaitu sebanyak 3 kelompok perlakuan sediaan tunggal teh hijau menggunakan konsentrasi 25%, 50%, dan 100%, sebanyak 4 kelompok perlakuan sediaan tunggal madu menggunakan konsentrasi 6,25%, 12,5%, 25%, dan 50%, sebanyak 12 dengan kelompok perlakuan sediaan kombinasi teh hijau dan madu. Jumlah koloni yang tumbuh pada media isolasi sebelum dan sesudah berkumur dihitung menggunakan alat colony counter. Hasil uji Repeated anova menunjukan nilai p = 0,037 (p < 0,05), terdapat perbedaan yang bermakna diantara perlakuan. Dapat disimpulkan bahwasediaan kombinasi teh hijau 50% dengan madu 12,5% dan teh hijau 100% dan madu 25% lebih efektif dalam menurunkan jumlah koloni bakteri.

INHIBITORY TEST OF FLAVONOID PROPOLIS KELULUT EXTRACTS (G. thorasica) ON Porphyromonas gingivalis AS AN ETIOLOGIC FACTOR OF CHRONIC PERIODONTITIS Valentina, Ursula; Oktiani, Beta Widya; Panjaitan, Fransiska Uli Arta
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7065

Background: Natural ingredients have been used recently for its ability in curing various diseases and reducing the risk of antibiotic resistance. One of these natural ingredients is propolis. Bee propolis Geniotrigona thorasica from South Kalimantan has a strong antibacterial activity with active compounds such as flavonoids. Flavonoids can damage cell membranes and can fight the expansion of Î²-lactamase enzyme produced by gram-negative bacteria. Porphyromonas gingivalis is a gram-negative bacterium that causes chronic periodontitis with a prevalence of 80%. Objective: This study was to find out the inhibitory test of flavonoid propolis kelulut extract at the concentration of 0.1%; 0.3% and 0.5% on Porphyromonas gingivalis bacteria. Method: This type of research used a pure experimental method with post-test only and control group design. One-way ANOVA statistical test was performed with follow-up test of Post Hoc Dunnetâ€™s T3. Treatment was given to 4 groups with 5 repetitions. Observations were carried out using digital calipers in all groups after incubation for 24 hours with a temperature of 37â„ƒ. Results: One Way Anova and Post Hoc Dunnet's T3 showed significant differences in the diameter flavonoid propolis extract inhibitory zone against Porphyromonas gingivalis. The average value of Flavonoid extract inhibitory zone after 24 hours observation with a concentration of 0.5%; 0.3%; 0.1% and sterile distilled water were 25.24 mm; 18.04 mm; 13.58 mm and there was no inhibitory zone observed in sterile distilled water group. Conclusion: Extract of flavonoid propolis kelulut at the concentration of 0.1%; 0.3% and 0.5% can inhibit the growth of Porphyromonas gingivalis as an etiologic factor of chronic periodontitis.

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Contact Name Rifky Ananda

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Location Kota banjarmasin, Kalimantan selatan INDONESIA

Abstract

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Contact Name
Rifky Ananda

Contact Email
fkg@ulm.ac.id

Phone
-

Journal Mail Official
dentino.ulm@gmail.com

Editorial Address
-

Location
Kota banjarmasin,

Kalimantan selatan

INDONESIA