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Dental Journal (Majalah Kedokteran Gigi)

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Published by Universitas Airlangga

ISSN : 19783728 EISSN : 24429740 DOI : -

Core Subject : Health,

Dentistry

The Dental Journal (Majalah Kedokteran Gigi) (e-ISSN:2442-9740; p-ISSN:1978-3728) is published by the Faculty of Dental Medicine, Universitas Airlangga. Its diciplinary focus is dental science and dental hygiene. The Dental Journal (Majalah Kedokteran Gigi) is published in English on a quarterly basis with each 50-60 page edition containing between nine and eleven scientific articles on research, study literature and case studies. Contributors to the Dental Journal (Majalah Kedokteran Gigi) included: dental researchers, dental practitioners, lecturers, and students drawn from Indonesia and a wide range of other countries.

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Articles 950 Documents

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Compressive strength resin komposit hybrid post curing dengan light emitting diode menggunakan tiga ukuran lightbox yang berbeda Mirza Aryanto; Milly Armilia; Dudi Aripin
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 2 (2013): June 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: The use of different polymerization methods may result in variation of mechanical properties of composite resin. Polymerization increases the conversion rate of monomers reflecting in improvement of compressive strength. Post-curing methods can be used to increase strength to the composite resin. Purpose: To determine the difference of compressive strength of post cured hybrid composite resin by using three different size of lightbox. Methods: This research was conducted in a true in vitro experiment. Research carried out by making a tube-shaped cylinder hybrid with 3 mm diameter and 6 mm height composite resin samples post cured by using 3 different size of light box, 3 cm x 3 cm x 3 cm (A), 4 cm x 4 cm x 4 cm (B) 6 cm x 6 cm x 6 cm (C), and a non post-curing control. Compressive strength test was then performed using a universal testing machine. Each sample was tested and averaged to obtain values in order to be analyzed statistically using ANOVA and multiple comparison. Results: There is an increase in compressive strength of each group, namely group A (172.9460 MPa), B (154.821 MPa), C (154.0789 MPa) and control (123.3550 MPa), and a statistically significant difference (F<0.05). Conclusion: The smaller size of the lightbox is used, the higher the compressive strength of composite resin.Latar belakang: Penggunaan berbagai metode polimerisasi dapat mengubah sifat mekanis resin komposit. Proses polimerisasi dapat meningkatkan derajat konversi monomer, sehingga dapat meningkatkan compressive strength resin komposit. Metode post curing dapat digunakan untuk menambah kekuatan resin komposit. Tujuan: Untuk mengetahui perbedaan compressive strength resin komposit hybrid yang dilakukan post curing menggunakan tiga ukuran lightbox yang berbeda. Metode: Jenis penelitian ini adalah eksperimental murni yang dilakukan secara in vitro. Penelitian dilakukan dengan membuat sampel resin komposit hybrid berbentuk tabung silinder dengan diameter 3 mm dan tinggi 6 mm yang dilakukan post curing menggunakan lightbox ukuran 3 cm x 3 cm x 3 cm (A), lightbox ukuran 4 cm x 4 cm x 4 cm (B), lightbox ukuran 6 cm x 6 cm x 6 cm (C), dan kelompok kontrol yang tidak dilakukan post curing. Kemudian dilakukan uji compressive strength dengan menggunakan alat uji universal testing machine. Data dianalisis secara statistik menggunakan ANOVA dan multiple comparison. Hasil: Terdapat peningkatan compressive strength pada tiap kelompok, yaitu kelompok A (172,9460 MPa), B (154,821 MPa), C (154,0789 MPa) dan kontrol (123,3550 MPa), dan secara statistik terdapat perbedaan yang signifikan (p<0,05). Simpulan: Semakin kecil ukuran lightbox yang digunakan, semakin tinggi tingkat compressive strength resin komposit hybrid.

Treatment of sharp mandibular alveolar process with hybrid prosthesis Sukaedi Sukaedi; Eha Djulaeha
Dental Journal (Majalah Kedokteran Gigi) Vol. 43 No. 3 (2010): September 2010
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Losing posterior teeth for a long time would occasionally lead to the sharpening of alveolar process. The removable partial denture usually have problems when used during mastication, because of the pressure on the mucosa under the alveolar ridge. Purpose: The purpose of this case report was to manage patients with sharp mandibular alveolar process by wearing hybrid prosthesis with extra coronal precision attachment retention and soft liner on the surface base beneath the removable partial denture. Case: A 76 years old woman visited the Prosthodontic Clinic Faculty of Dentistry Airlangga University. The patient had a long span bridge on the upper jaw and a free end acrylic removable partial denture on the lower jaw. She was having problems with mastication. The patient did not wear her lower denture because of the discomfort with it during mastication. Hence, she would like to replace it with a new removable partial denture. Case management: The patient was treated by wearing a hybrid prosthesis with extra coronal precision attachment on the lower jaw. Soft liner was applied on the surface of the removable partial denture. Hybrid prosthesis is a complex denture consisting of removable partial denture and fixed bridge. Conclusion: It concluded that after restoration, the patient had no problems with sharp alveolar process with her new denture, and she was able to masticate well.Latar belakang: Kehilangan geligi posterior dapat menimbulkan processus alveolaris tajam. Gigi tiruan sebagian lepasan mempunyai masalah selama pengunyahan karena adanya tekanan di mukosa di bawah alveolar ridge. Tujuan: Tujuan laporan kasus ini adalah untuk menjelaskan cara menangani pasien yang mempunyai prosesus alveolaris yang tajam di rahang bawah dengan dibuatkan protesis hybrid dengan daya tahan extra coronal precision attachment dan soft liner di permukaan bawah basis gigi tiruan sebagian lepasan. Kasus: Pasien wanita berumur 76 tahun datang di klinik Prostodosia Fakultas Kedokteran Gigi Universitas Airlangga. Pasien memakai gigi tiruan lekat rentang panjang di rahang atas dan gigi tiruan sebagian lepasan akrilik free end di rahang bawah, pasien mengalami masalah waktu mengunyah. Pasien tidak memakai gigitiruan lepasan rahang bawahnya karena tidak nyaman dipakai, dan pasien menginginkan pembuatan gigi tiruan lepasan rahang bawah yang baru. Tatalaksana kasus: Pada pasien ini dilakukan pembuatan Hybrid Prosthesis dengan daya tahan berupa extra coronal attachment di rahang bawah dan penggunaan bahan pelapis lunak yang diaplikasikan pada basis gigi tiruan lepasan nya. Hybrid prosthesis adalah gigi tiruan himpunan yang terdiri dari gigi tiruan lepasan dan gigi tiruan lekat. Kesimpulan: Hasil perawatan menunjukkan setelah mengganti gigi tiruan dengan gigi tiruan sebagian lepasan yang baru, pasien tidak mempunyai masalah dengan gigi tiruan yang baru akibat processus alveolaris yang tajam dan pasien dapat mengunyah dengan baik.

Antitumor activity of intratumoral injection of pcDNA3.1-p27Kip1mt followed by in vivo electroporation in a malignant Burkitt’s lymphoma cell xenograft Supriatno Supriatno; Sartari Entin Yuletnawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 4 (2012): December 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Human malignant Burkitt’s lymphomas are an uncommon type of Non-Hodgkin Lymphoma commonly affects in children. It is a highly aggressive type of B-cell lymphoma. Treatment for this malignant are still limited. However, a new strategy for refractory cancer, gene therapy is watched with keen interest. Recently, a novel method for high-efficiency and region-controlled in vivo gene transfer was developed by combining in vivo electroporation and plasmid cDNA. In the present study, a non-viral gene transfer system, in vivo electroporation in human malignant Burkitt’s lymphoma (Raji) cell xenograft was investigated. Purpose: The purpose of this study was to evaluate p27Kip1 gene therapy in Raji cell xenografts using pcDNA3.1-p27Kip1 mutant type (mt) and pcDNA3.1 empty vector (neo) with the local application of electric pulses. Methods: True experimental study using post-intervention with control group design was performed in this study. Material sample was obtained from integrated research laboratory at faculty of dentistry, Universitas Gadjah Mada, Yogyakarta. The efficiency of transfection of exogenous p27Kip1 gene by electroporation was confirmed by Western bloting analysis. To evaluate the reduction of malignant Burkitt’s lymphoma cell xenografts by this method, the volume of Raji cell xenografts in mice after electroporation with p27Kip1 mt or neo gene was measured. Results: Up-regulation of p27Kip1 protein was detected in pcDNA3.1-p27Kip1 mt. Furthermore, the growth of tumors was markedly suppressed by p27Kip1 mt gene transfection compared with transfection of neo. Conclusion: Injection of pcDNA3.1-p27Kip1 mt gene followed by in vivo electroporation has a high-potentially to suppress the growth of malignant Burkitt’s lymphoma cells. Furthermore, combination system of pcDNA3.1-p27Kip1 mt-injected tumor and electroporation might be used for human oral cancer. Latar belakang: Limfoma Burkitt’s maligna banyak terjadi pada anak-anak dan merupakan jenis yang langka dari limfoma NonHodgkin (NHL). Limfoma Burkitt’s maligna adalah tipe yang sangat agresif dari limfoma sel B. Perawatan penyakit ini masih sangatterbatas, walaupun demikian strategi baru perawatan kanker menggunakan terapi gen menjadi pusat perhatian. Suatu metode baru transfer gen untuk meningkatkan efisiensi dan kontrol area telah dikembangkan dengan mengkombinasi elektroporasi in vivo dan plasmid cDNA. Pada penelitian ini, telah diteliti sistim transfer gen non-virus dengan elektroporasi in vivo terhadap xenograft sel limfoma Burkitt’s maligna (sel Raji). Tujuan: Tujuan dari penelitian ini adalah untuk mengevaluasi terapi gen p27Kip1 terhadap xenograft sel Raji menggunakan pcDNA3.1-p27Kip1 mutant type (mt) dan pcDNA3.1 empty vector (neo) dengan aplikasi lokal elektroporasi. Metode: Jenis penelitian yang digunakan adalah eksperimen murni memakai rancangan pasca intervensi dengan kelompok kontrol. Sampel dan bahan penelitian didapat dari laboratorium riset terpadu, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta. Efisiensi transfeksi gen p27Kip1eksogen dengan elektroporasi dilakukan dengan analisis Western bloting. Untuk mengevaluasi hambatan xenograft sel limfoma Burkitt’s maligna dengan metode elektroporasi, dilakukan pengukuran volume xenograft sel Raji pada tikus pasca elektroporasi dan injeksi gen p27Kip1 mt atau neo. Hasil: Peningkatan regulasi protein p27Kip1 terdeteksi pada gen pcDNA3.1-p27Kip1 mt. Selanjutnya, pertumbuhan tumor secara signifikan terhambat oleh transfeksi gen p27Kip1 mt dibandingkan dengan transfeksi neo. Kesimpulan: Injeksi gen pcDNA3.1-p27Kip1 mt disertai elektroporasi in vivo mempunyai potensi yang kuat menghambat pertumbuhan "> sel limfoma Burkitt’s maligna. Kombinasi sistim injeksi tumor menggunakan gen pcDNA3.1-p27Kip1 mt dan elektroporasi kemungkinan dapat digunakan untuk terapi kanker oral.

Shear bond strength of self-adhering flowable composite on dentin surface as a result of scrubbing pressure and duration Ferry Jaya; Siti Triaminingsih; Andi Soufyan S; Yosi Kusuma Eriwati
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 3 (2012): September 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Self-adhering flowable composite is a combination of composite resin and adhesive material. Its application needs scrubbing process on the dentin surface, but sometimes it is difficult to determine the pressure and duration of scrubbing. Purpose: This study was aimed to analyze the effect of scrubbing pressure and duration on shear bond strength of self-adhering flowable composite to dentin surface Methods: Fifty four mandibulary third molar were cut to get the dentin surface and divided into nine groups (n = 6). Dentin surface was scrubbed with 1, 2, and 3 grams of scrubbing pressure, each for 15, 20, and 25 seconds respectively. surface was scrubbed with 1, 2, and 3 grams of scrubbing pressure, each for 15, 20, and 25 seconds respectively. Composite resin was applied incrementally and polymerized for 20 seconds. All specimens were immersed in saline solution at 37º C for 24 hours. Shear bond strength was tested for all specimens by using Universal Testing Machine (Shimadzu AG-5000E, Japan) at a crosshead speed of 1 mm/minute and analyzed by ANOVA and Post Hoc Test Bonferonni. The interface between self-adhering flowable interface between self-adhering flowable composite and dentin was observed with a Scanning Electron Microscope (JEOL JSM 6510LA). Results: The highest shear bond strength was obtained by 3 grams scrubbing pressure for 25 seconds or equal to applying the brush applicator in 0º relative to dentin surface. Conclusion: Increasing the scrubbing pressure and duration will increase the shear bond strength of self adhering flowable composite resin to dentinal surface. The highest shear bond strength was obtained when the applicator in 0º relative to dentin surface. Latar belakang: Self-adhering flowable composite merupakan gabungan resin komposit dengan material adhesif yang dalam penggunaannya memerlukan teknik scrubbing pada permukaan dentin, namun sulit untuk menentukan besar tekanan yang tepat saat scrubbing. Tujuan: Tujuan dari penelitian ini adalah untuk menganalisa pengaruh tekanan dan lama scrubbing saat aplikasi selfadhering flowable composite terhadap shear bond strength pada permukaan dentin. Metode: 54 gigi molar-3 rahang bawah dipotong sampai permukaan dentin dan dibagi atas 9 kelompok (n = 6). Scrubbing pada permukaan dentin dilakukan dengan besar tekanan 1, 2, dan Scrubbing pada permukaan dentin dilakukan dengan besar tekanan 1, 2, dan 3 gram, masing-masing selama 15, 20, dan 25 detik. Resin komposit diaplikasikan secara inkremental dan dipolimerisasi dengan sinar selama 20 detik. Spesimen direndam dalam larutan salin dan disimpan dalam inkubator pada suhu 37º C selama 24 jam. Shear bond strength diuji menggunakan Universal Testing Machine. Analisa data melalui uji ANOVA diikuti Post Hoc Test Bonferonni. Antarmuka self-adhering flowable composite resin dan dentin diamati melalui Scanning Electron Microscope (SEM). Hasil: Shear bond strength tertinggi antara self-adhering flowable composite resin dan dentin diperoleh pada tekanan scrubbing 3 gram selama 25 detik atau kuas membentuk sudut 0º dengan permukaan dentin. Kesimpulan: Semakin besar tekanan dan lama waktu scrubbing akan meningkatkan shear bond strength material self-adhering flowable composite resin terhadap permukaan dentin. Kekuatan paling tinggi diperoleh bila kuas aplikator membentuk sudut 0º dengan permukaan dentin selama proses scrubbing.

The increasing of beta-defensin-2 level in saliva after probiotic Lactobacillus reuteri administration Tuti Kusumaningsih
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 1 (2015): March 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Commesal bacteria is an excellent inducer for beta defensin-2 (BD-2). Probiotics bacteria Lactobacillus reuteri (L. reuteri) as commensal bacteria may play the same role as an excellent inducer for BD-2. Beta defensin is natural antimicrobial peptides widely expressed in oral cavity, including in epithelium salivary gland. Streptococcus mutans (S. mutans) as the main of bacteria causing caries are sensitive to BD-2. Purpose: This research was aimed to determine whether administration of probiotic L. reuteri can increase salivary BD-2 level in Wistar rats. Methods: This research can be considered as a laboratory experimental research with a randomized control group post test only design. Twenty-four male Rattus norvegicus Wistar strain rats aged 3 months were used. They were randomly divided into four groups, namely two control groups (negative control group that was not induced and positive control group induced with S. mutans), and two treatment groups (K1: induced with L. reuteri for 14 days and S. mutans for 7 days, and K2: induced with L. reuteri and S. mutans simultaneously for 14 days). L. reuteri culture at a concentration of 108 CFU/ml and S. mutans culture at a concentration of 1010CFU/ml were induced into the oral cavity of Wistar rats. An examination of BD-2 level was then conducted by using Elisa techniques. results: There was significant difference of salivary BD-2 level among those treatment groups (p=0.001). BD-2 level in saliva was increased after the administration of L. reuteri. Conclusion: L. reuteri probiotic can increase salivary BD-2 level in Wistar rats.

The effective concentration of red betel leaf (Piper crocatum) infusion as root canal irrigant solution Fani Pangabdian; Slamet Soetanto; Ketut Suardita
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 1 (2012): March 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Smear layer is a debris consisting of organic and inorganic particles of calcified tissue, necrotic tissue, pulp tissue, and dentinoblast and microorganism processes that can close the entrance to the dentin tubuli. Smear layer, will not only inhibit the penetration of disinfection materials and sealers to the dentin tubuli, but will also reduce the attachment of root canal filling material so that root canal irrigation solution is needed to dissolve the smear layer. Red betel leaf (Piper crocatum) infusion, on the other hand, contains saponin characterized as “surfactants” which can dissolve smear layer. Nevertheless, the effective concentration of the red betel leaf infusion has still not been known clearly. Purpose: This study is aimed to determine the effective concentration of the red betel leaf infusion for cleaning root canal walls from smear layer. Methods: Fiveteen extracted human teeth with straight single roots were randomized into 5 groups (n=3). The specimens were then shaped by using rotary instruments up to a size of 25/.07. During instrumentation, each canal was irrigated with 10, 20, 30 and, 40% red betel leaf infusion for treatment groups, while another was irrigated with aquadest for the control group. Root canal cleanliness was observed by using scanning electron microscope (SEM). Results: There were significant differences among treatment groups (p<0.05), except in the treatment groups irrigated with red betel leaf infusion with concentrations of 30% and 40% (p>0.05). Conclusion: It can be concluded that red betel leaf infusion with a concentration of 30% is effective for cleaning the root canal walls from the smear layer.Latar belakang: Smear layer adalah suatu debris yang mengandung partikel organik dan anorganik dari jaringan terkalsifikasi, jaringan nekrotik, proses dentinoblas, jaringan pulpa dan mikroorganisme yang dapat menutup jalan masuk ke tubuli dentin. Smear layer akan menghalangi penetrasi dari bahan disinfeksi dan sealer terhadap tubuli dentin dan mengurangi perlekatan bahan pengisi saluran akar, sehingga dibutuhkan larutan irigasi yang dapat membuang smear layer tersebut. Infusa daun sirih merah (Piper crocatum) mengandung saponin yang dikarakteristikkan sebagai surfaktan yang dapat melarutkan smear layer, tetapi sampai sekarang belum ada penelitian tentang hal tersebut. Tujuan: Penelitian ini dilakukan untuk mengetahui konsentrasi efektif daya pembersih infusa daun sirih merah (Piper crocatum) dapat membersihkan dinding saluran akar dari smear layer. Metode: 15 gigi premolar bawah manusia yang mempunyai akar lurus dibagi menjadi 5 kelompok secara acak (n=3). Gigi dipreparasi menggunakan rotary instrumen sampai Protaper F2 (30/0.02). Selama instrumentasi, dilakukan irigasi dengan infusa daun sirih merah (Piper crocatum) dan konsentrasi 10, 20, 30, 40% dan diirigasi aquadest untuk grup kontrol. Setelah itu kebersihan dinding saluran akar diperiksa dengan menggunakan scanning electron microscope (SEM). Hasil: Terdapat perbedaan yang signifikanantara masing-masing kelompok (p<0,05), kecuali kelompok yang diirigasi infusa daun sirih merah (Piper crocatum) konsentrasi 30% dan 40% (p>0,05). Kesimpulan: Dapat disimpulkan infusa daun sirih merah (Piper crocatum) dengan konsentrasi 30% efektif untuk membersihkan dinding saluran akar dari smear layer.

Oral rinse as a potential method to culture Candida isolate from AIDS patients Desiana Radithia; Hening T. Hendarti; Bagus Soebadi
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 4 (2011): December 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Candida isolate is easily sampled from oral cavity by swabbing directly on the candidiasis lesion, to be smeared onto slides for direct examination or cultured in a growth medium. This method is by far the gold standard for defining candidiasis diagnosis. However it is difficult to apply on sensitive patients and almost impossible on patients showing no clinical appearance of oral candidiasis. AIDS patients are very prone to candida infection and have a tendency of repetitive infection involving mixed species. As many candida species show different susceptibility to anti-fungal agents, it is necessary to identify the species causing the infection in the management of oral candidiasis. Oral rinse is a suggested method to obtain candida isolate to be cultured for further analysis such as species identification. This method is simple and less risky on infection transmission as less tools are required in the procedure. Purpose: This study aimed to assess the application of oral rinse as an alternative method to culture Candida isolate from AIDS patients. Methods: A cross-sectional observative study was conducted in HIV/AIDS in-Patient Facility of Intermediate Care Unit for Infection Disease, Dr. Soetomo Hospital Surabaya. Fourteen stadium 4 AIDS patients matching criteria were swabbed on 1/3-posterior of the tongue, and then given 10 ml phosphate buffer saline to rinse vigorously for 15 seconds. Both specimens were cultured on Sabouraud’s dextrose agar and colony growth was observed. Results: Candida colonies were able to grow from all 14 isolates (100%) by both methods. Qualitatively, cultures from oral rinse specimens were more populated than cultures from swab specimens. Conclusion: Oral rinse is an applicable technique to obtain Candida species isolate. This technique is safe, easy, non-invasive, and needs less tools therefore less risky for HIV transmission.Latar belakang: Isolat Candida mudah diambil dengan cara mengusap lesi candidiasis, baik untuk dioleskan pada kaca preparat untuk pemeriksaan langsung maupun dikultur. Hingga kini, metode tersebut dinyatakan sebagai “standar emas” untuk menentukan diagnosis. Namun kekurangan metode ini yaitu berisiko merangsang muntah pada pasien sensitif, dan hanya bisa dilakukan bila tampak jelas ada lesi. Candidiasis adalah penyakit nosokomial yang sering terjadi. Pasien AIDS sangat rentan terhadap infeksi oportunis ini secara rekuren dan persisten. Identifikasi spesies penyebab harus dilakukan karena berbagai spesies Candida memiliki kerentanan yang berbeda terhadap berbagai jenis antifungal. Oral rinse adalah metode pengambilan isolat Candida yang non-invasif. Isolat yang didapat bisa dikultur dan diidentifikasi, selain itu bisa dilakukan pada pasien yang belum menunjukkan adanya lesi candidiasis untuk menentukan besar risiko pasien terkena candidiasis, sehingga dapat ditentukan perlu tidaknya pemberian profilaksis antifungal. Tujuan: Penelitian ini bertujuan mengamati efektivitas metode oral rinse untuk mengisolasi Candida dari rongga mulut pasien AIDS. Metode: Penelitian observasional dilakukan di bagian Rawat Inap Unit Perawatan Intermediate Penyakit Infeksi RSUD Dr. Soetomo. Swab pada 1/3 posterior lidah dilakukan pada 14 pasien yang memenuhi kriteria, kemudia pasien diberi 10 ml phosphate buffer saline untuk berkumur kuat-kuat selama 15 detik. Spesimen yang didapat melalui kedua metode dikultur pada medium Sabouraud untuk diamati. Hasil: Koloni Candida berhasil dikultur dari 14 spesimen (100 %) melalui kedua metode isolasi. Secara kualitiatif tampak bahwa hasil kultur dari oral rinse tampak lebih subur. Kesimpulan: Oral rinse adalah metode yang dapat diaplikasikan untuk mengisolasi Candida dari rongga mulut pasien. Tekniknya mudah, aman, non-onvasif, dan tidak memerlukan peralatan dan ketrampilan khusus, sehingga mengurangi risiko transmisi HIV.

Evaluasi karakteristik abu sekam padi dengan kitosan molekul tinggi nanopartikel sebagai bahan dentinogenesis (Characteristic evaluation of rice husk ash with chitosan high molecule nanoparticle as dentinogenesis material) Pretty Farida Sinta Silalahi; Trimurni Abidin; Harry Agusnar
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 2 (2014): June 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Mineral trioxide aggregate (MTA) and resin modified glass ionomer cement (RMGIC) are the material used for indirect and direct pulp capping due to biocompatibility, but these materials have many shortcomings. Mineral trioxide aggregate contains a little amount of arsenic and has long setting time, while HEMA containing RMGIC are cytotoxic. Rice husk ash nanoparticles (RHAn) is a potential source of silica. High molecular chitosan nanoparticles (HMCn) can stimulate the formation of reparative dentin. Combination of these two materials is biocompatible and have good sealing ability. Purpose: This study was aimed to study RHAn + HMCn used as biomaterials for prevention of pulpodentinal complex by examined at the microstructure of dentin surfaces applied with RHAn + HMCn. Methods: Twenty-four mandibular premolar teeth extracted for orthodontic purposes, were made cavity class I preparation with 3 mm depth above the cemento-enamel junction (CEJ). Then each tooth was cut in bucco-lingual direction and each part was cut using a cervical disc bur. Samples were divided into 3 groups, group I the teeth were applied with MTA; group II the teeth were applied RMGIC; group III the teeth were apllied with RHAn + HMCn. Characterization was done by using Scanning Electron Microscopy (SEM) on the interface between test material and dentin adjacent to the pulp to see surface microstructure. Results: Material microstructure of RHAn + HMCn applied to the dentine showed tags like structure which was more significant than MTA. RHAn + HMCn showed to have better sealing ability than MTA. Porosity of ASPn + HMCn was less than MTA and RMGIC. Conclusion: The study suggested that the combined RHAn + HMCn biomaterials could be used as an active biomaterial that can maintain the integrity of pulp dentinal complex.Latar belakang: Mineral trioksida agregat (MTA) dan semen ionomer kaca modifikasi resin (SIKMR) adalah bahan yang digunakan untuk pulp capping langsung dan tidak langsung karena biokompatibel, namun bahan ini memiliki banyak kekurangan. Trioksida Mineral agregat mengandung sejumlah kecil arsenik dan setting time-nya lama, sementara HEMA dalam SIKMR bersifat sitotoksik. Abu sekam padi nanopartikel (ASPn) merupakan sumber potensial dari silika. Kitosan molekul tinggi nanopartikel (KMTn) dapat merangsang pembentukan dentin reparatif. Kombinasi dari kedua bahan tersebut memiliki sifat biokompatibel dan memiliki kemampuan pelapisan yang baik. Tujuan: Penelitian ini bertujuan untuk menganalisa karakteristik mikrostruktur hubungan permukaan abu sekam padi dengan kitosan molekul tinggi nanopartikel pada jaringan dentin untuk melindungi jaringan pulpodential kompleks. Metode: Dua puluh empat gigi premolar mandibula yang diekstraksi untuk tujuan ortodontik digunakan sebagai sampel, gigi dibuat preparasi kavitas klas I dengan kedalaman 3 mm di atas cemento enamel junction (CEJ). Kemudian masing-masing gigi dibelah dua arah bucco-lingual dan setiap bagian dipotong menggunakan disc bur servikal. Sampel dibagi 3 kelompok, kelompok I diaplikasikan MTA, kelompok II diaplikasikan SIKMR, kelompok III diaplikasikan ASPn + KMTn. Karakterisasi dilakukan dengan menggunakan Scanning Electron Microscopy (SEM) pada interface antara bahan uji dan dentin berdekatan dengan pulp untuk melihat struktur mikro permukaan. Hasil: Microstructure bahan ASPn + KMTn yang diaplikasi pada dentin menunjukkan struktur seperti tag yang lebih signifikan daripada MTA. ASPn + KMTn menunjukkan kemampuan pelapisan yang lebih baik dari MTA. Porositas ASPn + KMTn lebih sedikit dari MTA dan SIKMR. Simpulan: Penelitian ini menunjukkan bahwa kombinasi biomaterial ASPn + KMTn dapat digunakan sebagai biomaterial aktif yang dapat menjaga integritas pulpa.

Anterior makeover on fractured teeth by simple composite resin restoration Eric Priyo Prasetyo
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 3 (2011): September 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: In daily practice dentists usually treat tooth fractures with more invasive treatments such as crown, veneer and bridges which preparation require more tooth structure removal. While currently there is trend toward minimal invasive dentistry which conserves more tooth structure. This is enhanced with the vast supply of dental materials and equipment in the market, including restorative materials. Provided with these supporting materials and equipment and greater patient’s demand for esthetic treatment, dentists must aware of the esthetics and basic principle of conserving tooth which should retain tooth longevity. Purpose: This article showed that a simple and less invasive composite resin restoration can successfully restore anterior esthetic and function of fractured teeth which generally treated with more invasive treatment options. Case: A 19 year-old female patient came with fracture on 21 and 22. This patient had a previous history of dental trauma about nine years before and was brought to a local dentist for debridement and was given analgesic, the involved teeth were not given any restorative treatment. Case management: The fractured 21 and 22 were conventionally restored with simple composite resin restoration. Conclusion: Fracture anterior teeth would certainly disturbs patient’s appearance, but these teeth could be managed conservatively and economically by simple composite resin restoration.Latar belakang: Dalam praktek sehari-hari pada umumnya dokter gigi merawat fraktur dengan restorasi invasif seperti mahkota, veneer dan jembatan yang semuanya memerlukan pengambilan jaringan gigi lebih banyak, sedangkan saat ini trend perawatan gigi lebih menuju kearah invasif minimal yang mempertahankan jaringan gigi sebanyak mungkin. Keadaan ini ditunjang oleh tersedianya berbagai macam bahan dan peralatan kedokteran gigi di pasaran, termasuk bahan restorasi. Dengan tersedianya bahan dan peralatan yang mendukung serta tingginya permintaan pasien akan perawatan estetik, dokter gigi harus mengetahui estetik dan prinsip dasar dari konservasi gigi yaitu mempertahankan gigi selama mungkin. Tujuan: Laporan kasus ini menunjukkan bahwa restorasi resin komposit sederhana yang tidak invasif dapat memperbaiki estetik dan fungsi geligi fraktur yang umumya dirawat dengan macam restorasi yang lebih invasif. Kasus: Seorang wanita berusia 19 tahun datang dengan fraktur pada gigi 21 dan 22. Pasien tersebut memiliki riwayat trauma pada giginya sejak sembilan tahun sebelumnya dan telah dibawa ke dokter gigi untuk debridemen dan pemberian analgesik saja, tanpa perawatan restoratif. Tatalaksana kasus: Gigi fraktur pada 21 dan 22 diperbaiki dengan restorasi resin komposit sederhana. Kesimpulan: Fraktur pada gigi anterior akan sangat mengganggu penampilan pasien, akan tetapi fraktur tersebut dapat dirawat secara konservatif dan ekonomis dengan restorasi resin komposit sederhana.

Antimicrobial proteins of Snail mucus (Achatina fulica) against Streptococcus mutans and Aggregatibacter actinomycetemcomitans Herluinus Mafranenda DN; Indah Listiana Kriswandini; Ester Arijani R
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 1 (2014): March 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Achasin and mytimacin-AF are proteins of snail mucus (Achatina fulica) which have antimicrobial activity. Snail mucus is suspected to have other proteins which have antimicrobial activity against Streptococcus mutans and Aggregatibacter actinomycetemcomitans the oral pathologic bacteria. Purpose: The study were aimed to characterize the proteins of snail mucus (Achatina fulica) that have antimicrobial activities to Streptococcus mutans and Actinobacillus actinomycetemcomitans, and to compared the antimicrobial effect of achasin and mytimacin-AF. Methods: The sample of study was the mucus of snails which were taken from Yogyakarta Province. The isolation and characterization of protein were conducted by using SDS-PAGE method, electroelution, and dialysis. Nano drop test was conducted to determine protein concentration. The sensitivity test was conducted by using dilution test, and followed by spectrophotometry and paper disc diffusion tests. Results: The study showed that proteins successfully characterized from snail mucus (Achatina fulica) were proteins with molecular weights of 83.67 kDa (achasin), 50.81 kDa, 15 kDa, 11.45 kDa (full amino acid sequence of mytimacin-AF) and 9.7 kDa (mytimacin-AF). Based on the dilution test, Achasin had better antimicrobial activities against Streptococcus mutans, while mytimacin-AF had better antimicrobial activities against Aggregatibacter actinomycetemcomitans. But the paper disc diffusion test result showed that Achasin had antimicrobial activities against Streptococcus mutans and Aggregatibacter actinomycetemcomitans, while mytimacin-AF had no antimicrobial activities. Conclusion: The proteins with molecular weights of 50.81 kDa, 15 kDa, 11.45 kDa were considered as new antimicrobial proteins isolated from snail mucus. Achasin, had better antimicrobial activities against Streptococcus mutans, while mytimacin-AF had better antimicrobial activities against Aggregatibacter actinomycetemcomitans.Latar belakang: Achasin dan mytimacin-AF adalah protein lendir bekicot (Achatina fulica) yang memiliki aktivitas antimikroba. Lendir bekicot diduga memiliki protein lain yang memiliki aktivitas antimikroba terhadap Streptococcus mutans dan Actinobacillus actinomycetemcomitans bakteri patologis oral. Tujuan: Penelitian ini bertujuan untuk mengkarakterisasi protein lendir bekicot (Achatina fulica) yang memiliki aktivitas antimikroba terhadap Streptococcus mutans dan Aggretibacter actinomycetemcomitans, dan membandingkan efek antimikroba protein achasin dan mytimacin-AF. Metode: Sampel penelitian adalah lendir bekicot yang diambil dari Provinsi Yogyakarta. Isolasi dan karakterisasi protein dilakukan dengan metode SDS-PAGE, elektro-elusi, dan dialisis. Nano drop test dilakukan untuk menentukan konsentrasi protein. Uji sensitivitas dilakukan dengan menggunakan uji dilusi, dan diikuti oleh spektrofotometri dan tes difusi kertas cakram. Hasil: Protein dari lendir bekicot (Achatina fulica) yang ditemukan adalah protein dengan berat molekul 83,67 kDa (achasin), 50,81 kDa, 15 kDa, 11,45 kDa (urutan asam amino penuh mytimacin-AF) dan 9,7 kDa (mytimacin- AF). Berdasarkan uji dilusi, Achasin memiliki aktivitas antimikroba yang lebih baik terhadap Streptococcus mutans, sedangkan mytimacin-AF memiliki aktivitas antimikroba yang lebih baik terhadap Aggregatibacter actinomycetemcomitans. Namun hasil uji difusi cakram kertas menunjukkan bahwa Achasin memiliki aktivitas antimikroba terhadap Streptococcus mutans dan Aggegatibacter actinomycetemcomitans, sementara mytimacin-AF tidak memiliki kegiatan antimikroba. Simpulan: Protein dengan berat molekul 50,81 kDa, 15 kDa, 11,45 kDa merupakan protein antimikroba baru diisolasi dari lendir bekicot. Achasin, memiliki aktivitas antimikroba yang lebih baik terhadap Streptococcus mutans, sedangkan mytimacin-AF memiliki aktivitas antimikroba yang lebih baik terhadap Actinobacillus actinomycetemcomitans.

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Saka Winias

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