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Jurnal Veteriner

jvet
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Published by Universitas Udayana

ISSN : 14118327 EISSN : 24775665 DOI : https://doi.org/10.19087/jveteriner

Core Subject : Health,

Veterinary

Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki

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Articles 19 Documents

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Search results for , issue "Vol 20 No 4 (2019)" : 19 Documents clear

Respons Stres Sapi Bali Jantan pada Proses Pengangkutan dan Pemuatan ke Atas Kapal Ternak (STRESS RESPONSE OF BALI BULLS DURING TRANSPORTATION AND LOADING PROCCESS ONTO THE CATTLE SHIP) Sri Widayati; Puji Astuti; Irkham Widiyono
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Transportation is needed for livestock distribution from production centers to consumption centers. This might potentially result in a stressful condition for the cattle. The study was aimed to observe the stress response of Bali bulls during the transport and loading proccess onto the ship. Six adult clinically healthy Bali bulls were used in this study. The animals were housed in pen at the animal quarantine installation, given dried straw and drinking water ad libitum since three days before the study. The animals were loaded on the truck in 30 minutes, transported to the harbor in 15 minutes, and loaded on to the ship in 30 minutes. On the ship the animals were kept in a pen bedded with straw and given dried straw and drinking water ad libitum for 19 hours. Venous blood samples were collected from jugular vein during the rest in pen before loading on to the truck, after loading on the ship, and about 19 hours after loading and resting in the ship pen. Serum was separated by centrifugation and stored at -20oC for cortisol analysis. The concentrations of cortisol was analyzed by using enzyme linked immunosorbent assay (ELISA). The results showed that the average of serum cortisol concentration of Bali bulls was at the level of 33.81±28.52 ng/mL in the rest period at the quarantine installation, increased significantly to the level of 138.41±102.57 ng/mL after loading onto the ship (P<0.05), and decreased again to the level of 41.54±43.47 ng/mL after 19 hours of resting period in the pen on the ship (P>0.05). It can be concluded that Bali bulls may suffer from stress during transport and loading proccess which can be recovered after resting in the ship pen.

Deteksi Gen Penyandi Resistensi ampC dan mcr-1 pada Escherichia coli penyebab Colibacillosis Unggas di Sukabumi (DETECTION OF GENE ENCODING RESISTANCE AMPC AND MCR-1 IN ESCHERICHIA COLI CAUSES AVIAN COLIBACILLOSIS IN SUKABUMI) Agustin Indrawati; Ryan Septa Kurnia; Ni Luh Putu Ika Mayasari
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Antibiotic resistance has become a global problem that can threaten human and animal health. The use of antibiotics in livestock as a treatment and control of disease is often associated with the cause of the spread of resistant bacteria. Resistance bacteria are caused by presence of resistant gene resistance that can move between bacteria. This study aims to detect the presence of genes that encode resistance to ampicillin (ampC) antibiotics and colistin (mcr-1) in Escherichia coli bacteria derived from cases of colibacillosis in Sukabumi. A total of 25 isolates of E. coli archive collection of PT. Medika Animal Lab is used in this research. All isolates identified using PCR were then tested for sensitivity using the disk diffusion method and minimum inhibitory concentrations (MICs). Isolates that are resistant to ampicillin and colistin were tested for detection of ampC and mcr-1 genes using PCR. The results of the sensitivity test showed the whole isolates were resistant to ampicillin (100%) and phosphomycin (8%), but none were resistant to colistin sulphate. The total isolate E. coli successfully detected gene encoding resistance of ampC (100%). The results of sensitivity and resistance detection test showed that the whole isolates were ampicillin resistant and had the ampC resistance-encoding gene.

Dinamika Total Protein Serum Tikus Putih (Rattus novergicus) yang Diberi Mikrokapsul Imunoglobulin-G Anti-H5N1 (DYNAMICS OF TOTAL PROTEIN SERUM OF RATTUS NOVERGICUS GIVEN MICROCAPSULES OF IMMUNOGLOBULIN G ANTI H5N1) Kathirina Beatrik Riwu Wolo; Anita Esfandiari; Sri Murtini; Retno Wulansari
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Immunoglobulin G (IgG) from colostrum of hyperimmune colostrum obtained from vaccinated cattle with H5N1 vaccine is potential to be developed into passive immunotherapy product for “bird flu” infection. The administration of passive therapy can be done orally, but the environmental conditions of the digestive tract can cause IgG damage, so the microencapsulation technique was used in this study to protect IgG. This study aimed to evaluate the absobrption of H5N1 IgG anti-Avian Influenza which was microencapsulated using rats (Rattus novergicus) as animals model. This study was conducted using 108 adult male white rats (Rattus norvegicus), Wistar strain, approx. 250 grams of body weight. Rats were divided into three treatment groups (N = 36), i.e. control group (K) were given aqua distilled water, suspension group (S) were given anti-H5N1 IgG suspension, and microcapsules group (M) were given microcapsules equivalent to suspension with the titer of 25. Necropsy was carried out at four observation points (2, 4, 6 and 8 hours) to collected blood through the heart and collected in vacutainer without anticoagulants to obtain serum. The blood serum then analyzed for total protein and globulin and albumin/globulin ratio (A/ G). The results showed that the level of total protein and globulin group M was higher and significantly different (P> 0.05) compared to K and S at the end point of observation. The A/G ratio in group K showed a significant increase (P> 0.05) at the end point of observation while in group M there was a marked decrease (P> 0.05) at the time point of 2 hours to 4 hours and then constant until the end of observation. These results showed that administration of anti-H5N1 IgG microcapsules can increase total levels of protein and globulin in the blood.

Heart Rate of Reticulated Pythons (Malayopython reticulatus) from Bali based on Its Body Length (DETAK JANTUNG ULAR SANCA BATIK (Malayopython reticulatus) BERDASARKAN PANJANG TUBUH DI BALI) Steven Dwi Purbantoro; Puveanthan Nagappan Govendan; I Gusti Made Krisna Erawan; Zefanya Christiani; Erika Erika; Yedija Putra Kusuma Wardana Rumbay; Slamet Raharjo
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study was aimed to determine the correlation between heart rate and body length of reticulated python (Malayopython reticulatus) from the island of Bali. The study offers data of the M. reticulatus heart rate value to exotic animal veterinary practitioner. Fifteen clinically healthy snakes, consists of 9 females and 6 males were used in this study. Heart rate was measured twice a day during day and night using a fetal Doppler (BF-500 eBestman®, China) and body length using a measurement tape. Heart rate was analyzed with independent t-test to time collection and correlation to body length. Results show that heart rate mean value at day and night of the M. reticulatus from the island of Bali was each 65.68±14.093 bpm and 64.53±13.637 bpm. Heart rate value of wild-caught snakes is higher (67.90±15.290 bpm) than captivebred snakes (60.92±10.039 bpm). Total length (TL), snout-vent length (SVL), and snout-heart length (SHL) mean value of M. reticulatus from the island of Bali was each 168.6±90.67 cm, 149.53±82.77 cm, and 38.7±19.28 cm. Correlation between heart rate and total length has higher R-value (R = 0.802), and followed by snout-heart length (R = 0.800) and snout-vent length (R = 0.792). Statistically, it could be concluded that heart rate during day and night time shows no significant difference, heart rate value based on how it is kept is significantly different, and heart rate was negatively correlated to body length.

Karakteristik Fisik dan Nilai Organoleptik Sosis Daging Kuda Berdasarkan Level Subtitusi Tepung Tapioka (PHYSICAL CHARACTERISTICS AND ORGANOLEPTIC VALUESOF HORSE’S MEAT SAUSAGE BASED ON LEVEL SUBTITUTION OF TAPIOCA FLOUR) Bulkaini Bulkaini; Djoko Kisworo; Muhammad Yasin
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of the study was to determine the effect of the tapioca flour level substitution on physical characteristics and organoleptic values of sausage made of male horse’s meat.The material used was meat of 4.0-4.5 years old male horse and a number of spices in making sausages. This study was conducted in a One Way Completely Randomized Design with four treatments and three replications, namely P1 = Control (without tapioca flour); P2 = 15% tapioca flour; P3 = 30% tapioca flour and P4 = 45% tapioca flour. The data obtained were analyzed using analysis of variance. The treatments which significantly different were then continued analyzed using the Duncan New Multiple Range Test. The results showed that the level of tapioca flour substitution had a significant effect (P <0.05) on water holding capacity (WHC), color, aroma and texture of the sausage, while the cooking lost, tensile strength, pH, and taste were not affected significantly (P> 0.05). The substitution of 30% tapioca flour from the ingedients gave the highest value of horse’s meat sausages in WHC of (45.74 ± 8.18) %, but low in cooking losses of (3.33 ± 1.15) %, low tensile strength of (0.45 ± 0.00) kg/cm2 and normal pH (6.66 ± 0.05). Based on organoleptic values that substitution of 30% tapioca flour gave slightly brown color of sausage (3.83 ± 0.03), good aroma (5.95 ± 0.04), delicious taste (6.20 ± 0.03) and soft texture (5.68 ± 0.02).

Perkembangan Histologis Ovarium Bayi dan Anak Owa Jawa (Hylobates moloch) (HISTOLOGICAL DEVELOPMENT OF NEONATE AND JUVENILE JAVAN GIBBON (Hylobates moloch) OVARIES) Pristiani Nurantika Notosoediro; Adi Winarto; Ligaya Innocentia Theresia Antoinetta Tumbelaka
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Reproductive success is one of the biggest challenges for the existence of javan gibbon (Hylobates moloch) in the future. Basic biology information of main reproduction female organ of the species is yet unknown. The research aimed to provide information of female ovary development through histological examination. Two pairs of ovaries were collected from a neonate and a three years old female cadaver at Javan Gibbon Center. Histological techniques (cross and longitudinal sections) were applied to the collected samples using paraffin method with haematoxylin eosin (HE) and Masson’s trichrome (MT) dyes. The follicles are spread evenly in the left and right cortex ovary. The number of primordial follicles within the neonate and infant ovary was 80 815 and 34 622, respectively. In a three years old javan gibbon ovaries, the development of primordial into primary, pre-antral and antral follicles were observed. The average diameter of the follicles and oocytes were, respectively; 25.0±8.9 ?m and 20.0±13.1 ?m for primordial follicle, 48.4±22.5 ?m and 20.5±10.0 ?m for primary follicle, 79.0±49.0 ?m and 25.4±17.4 ?m for pre-antral follicle, 315.5±36.1 ?m and 32.0±17.0 ?m for antral follicle. The size of primordial and primary oocyte and follicle of javan gibbon is smaller than those of Macaca fascicularis at the same age. The connective tissue of neonate ovary was being developed while in the 3 years old female ovary, it was well-developed and clearly seen in the capsula, cortex, and medulla. Javan gibbon follicle development is strongly influenced by age.

Deteksi Anaplasma sp. pada Anjing di Bali secara Klinis, Serologis, dan Molekuler (THE DETECTIONS OF ANAPLASMA SP. IN DOGS IN BALI WITH CLINICAL, SEROLOGICAL AND MOLECULAR) Anak Agung Sagung Istri Pradnyantari; I Nyoman Suartha; I Gusti Made Krisna Erawan; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study aims to determine the infection of Anaplasma sp. in dogs in Bali with clinical, serological and molecular detections. This research method uses Cross Sectional. The samples were collected from June 2017 to July 2018. The total number of samples obtained was 109 dogs from 3.563 samples from seven veterinarian clinics in Bali. Clinical examinations and blood tests are method of clinical detection. Serological detection is using a rapid test kit E. canis & Anaplasma spp. BioNote© production. Molecular detection have been using with the Polymerase Chain Reaction technique. PCR products were sequenced at 1st BASE Laboratories Sdn Bhd, Malaysia. Data were analyzed using the MEGA 4 program. The results showed that Anaplasma sp. are present in dogs in Bali and can be detected by clinically (3,05%), serologically (55,04% base on clinically positive) and molecularly (42,20% base on clinically positive) detection. Thus, detected species Anaplasma sp. In Bali from this study can be identified as A. platys.

Karakteristik Molekuler Virus Avian Orthoavulavirus 1 Genotipe VII yang Diisolasi dari Tabanan Bali (MOLECULAR CHARACTERISTIC OF AVIAN ORTHOAVULAVIRUS 1 GENOTYPE VII ISOLATED FROM TABANAN BALI) Anak Agung Ayu Mirah Adi; I Nyoman Mantik Astawa; I Nengah Wandia; I Gusti Agung Arta Putra; Ida Bagus Oka Winaya; Anak Agung Keswari Krisnandika; Anak Agung Oka Wijaya
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Newcastle disease (ND) is a very harmful avian disease, endemic in Indonesia and various parts of the world. The causative agent is ND virus or Avian orthoavulavirus 1 (AOAV-1). This virus is an RNA virus with wide genetic variation. Based on the genome length, it can be classified into AOAV-1 Class I and II. Class I are generally avirulent whereas Class II are consist of both virulent and avirulent viruses, currently there are 18 genotypes of the class II. To find out the molecular characteristics of AOAV-1 currently circulating in the field, isolation and identification of viruses from laying hens that was suspected ND from Tabanan Bali in 2017, was performed. The isolated viruses hereafter named as Tabanan1/ARP / 2017. A one-step RT-PCR reaction was carried out to amplify NP, F and HN gene fragments from the virus using three specific pairs of AOAV-1 primers. The obtained nucleotide sequences are then used in phylogenetic analysis. For phylogenetic analysis several strains of AOAV-1 from class II representing genotype I-VII as well as one strain from Class I were accessed from GenBank. From the analysis of the F gene nucleotide sequences, it was found that Tabanan 1 / ARP / 2017 is a genotype VII virus with an amino acid sequence at the F protein cleavage site is 112 R-R-Q-K-R-F117, a typical virulent strain. Phylogenetic analysis using nucleotide sequences NP and HN genes also positioned this isolate in genotype VII. At the nucleotide level, genetic distance with virulent isolates that was isolated in 2007 and 2010 were 8.26% and 1.08% while at the amino acid level were 5.26% and 0.64%. There were found mutations in amino acids at positions 107 and 108 of F protein.

Respons Imun Puyuh (Coturnix coturnix Japonica) Dewasa yang Mendapat Ekstrak Daun Singkong dalam Mengatasi Dampak Cekaman Panas (IMMUNE RESPONSE OF ADULT QUAILS (COTURNIX COTURNIX JAPONICA) TREATED WITH CASSAVA LEAF EXTRACT TO OVERCOME HEAT STRESS) Koekoeh Santoso; Anindita Sista Widyadhari; Okti Nadia Poetri; La Jumadin
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of the research was to prove the potency of cassava leaf extract to overcome heat stress of adult quails on the variable of antibody titer of Newcastle Disease (ND), MDA level, and total protein. The research was divided into four groups and conducted in 6 replications for each group, consisting of control group, group A, B, and C. All the groups were exposed to heat stress, and then treated with cassava leaf extract with different dosages for 5,292 mg/168 g body weight, 10,584 mg/168 g body weight, and 21,168 mg/168 g body weight for 28 days after being adapted for a week. Variables of antibody titer of Newcastle Disease (ND), MDA level, and total protein were measured every week. The result showed that HI titer of overall tested groups was less than 2 HI units. HI titer contains positive antibody of antibody titer of ND if it reaches 16 HI units. HI test in the present study showed that all of quail serums contained negative antibody of ND. The level of MDA fluctuated with the highest and smallest value was found in group B and control group, respectively. In addition, the administration of cassava leaves extract tended to decrease total protein, where control group was significantly different to both group A and C (P < 0,05). In conclusion, cassava leaves extract has the potential to decrease the stress level, but the administration up to 21,168 mg/168 g BB has not been able to decrease the level of MDA in quails that suffered from heat stress. In Dosage 21, 168 mg extract of cassava leaves was potential to lower stress level so that it was unable to stimulate immune respond to form ND titer antibody.

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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

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Editorial Address Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

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I G. Made Krisna Erawan

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krisnaerawan@unud.ac.id

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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

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Kota denpasar,

Bali

INDONESIA