Jurnal Veteriner
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Pakan Tambahan dan Anabolik Growth Promoter Meningkatkan Kadar Hormon Pertumbuhan Sapi Bali
Ni Made Riska Adnyani;
Ni Ketut Suwiti;
I Gusti Ayu Agung Suartini;
I Nengah Kerta Besung
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
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DOI: 10.19087/jveteriner.2020.21.4.575
This study aims to determine the level of growth hormone given feed supplement and anabolic growth promoter. This research was an experimental research using Complete Random Design (CRD) with three factors. There were feed supplement, anabolic growth promoter, and sampling time. There were 20 youngmale Bali cattle and they were taken care intensively, divided into 4 group, there were control, feed supplement, anabolic growth promoter, and the combination of feed supplement and anabolic growth promoter for five months, and measured the level of growth hormone each month. The level of growth hormone was detected by using competitive Enzyme Linked Immunosorbent Assay method. The result of the research showed that feed supplement and anabolic growth promoter increased the level of bali cattle growth hormone where feed supplement with anabolic growth promoter (P1G1) was not significantlydifferent than given feed supplement without anabolic growth promoter (P1G0). Providing feed supplement optimally by farmers is highly recommended.
Ekstrak Kulit Buah Kakao (Theobroma cacao L.) Limbah Perkebunan Berpotensi sebagai Akarisida Alami Terhadap Caplak Boophilus microplus
Suryadi Pappa;
Abdul Wahid Jamaluddin;
Adryani Ris
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
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DOI: 10.19087/jveteriner.2020.21.4.611
This study was aims to exploit the potential waste of cocoa pod (Theobroma cacao L.) as a natural acaricide in ticks (Boophilus microplus). The study was conducted with a two-variable completely randomized design (CRD) with two repetitions. The sample does not differentiate between sex, body weight, and length of about 0.8 - 1 cm. Each 5 ticks were treated as follows: treat 1 extract of 10% cocoa pod husk; treatment 2 20% cocoa pod skin extract; treatment 3 30% cocoa pod husk extract; treatment 4 negative control; treat 5 positive controls. In the first variable the tick was treated with methanol extract, while in the second variable the ethanol extract was treated with 10%, 20%, 30% respectively, negative control and positive control. In treatment I used 25 ticks plus treatment II 25 ticks. Each treatment was repeated to avoid data bias so that a total of 100 ticks were used. The results showed that the cocoa pod husk extract had the best killing power at a concentration of 30% with methanol solvent at 6.21 hours while ethanol solvent at 5.91 hours. The use of extraction materials has not been able to compensate for the use of synthetic materials that can kill in minutes.
Perpanjangan Usia Tikus Tua Setelah Xenotransplantasi Human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs)
Elpita Tarigan;
Adisti Dwijayanti;
Frans Dhyanagiri Suyatna;
Indra Bachtiar;
Sandy Qlintang;
I Ketut Mudite Adnyane;
Arief Boediono
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
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DOI: 10.19087/jveteriner.2020.21.4.539
Currently, mesenchymal stem cells (MSCs) for implementing regenerative medicine in aging become interest in medical research science, especially in degerative disease and other aging problems. This research was aimed to determine the effectiveness of hUC-MSCs on inhibiting the aging process through the lifetime of the rat and the effect of intravenous administration of hUC-MSCs in phisiologycally aging female rat on the blood analysis. This study was used 40 aged female rats with 29-30 months of age divided into four groups with 10 rats each. The control rat group was given physiological NaCl (0.9%) 0.5 mL, and the treated rat group was given hUC-MSCs 1x107 cells/kg body weight in 0.5 mL NaCl 0.9%, wasinjected intravenously in caudo lateralis tail vein with stratified frequency; one time injection (SC1), three times injections (SC3) and five times injections (SC5). Perifer blood was collected from retro-ortbital sinus vein 30 days before and after injection of hUC-MSCs for hematology and blood chemistry analysis. Based on the results were obtained, it indicated that hUC-MSCs increased the survival of aging rat werein treatment group, life span of rats was extended up to 40 months compared to the average life of control rat aged up to 34±2 months. The injection of hUC-MSCs 1x107 cells/kg of body weight with one, three andfive times injection were affected to blood profiles and blood chemistry with correlation were low. The conclusions are hUC-MSCs extend the lifespan of aging rat and were affect the blood in general but in normal range of aging rat, affect in ALT and creatinin as tissue repair and tolerated by aging rat.
Perubahan Patologi Sistem Hepatobiliari Sapi Bali Terinfeksi Fasciola gigantica
Ida Bagus Oka Winaya;
Anak Agung Ayu Mirah Adi;
I Ketut Berata;
I Made Kardena;
Ida Bagus Windia Adnyana;
Ida Bagus Kade Suardana
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
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DOI: 10.19087/jveteriner.2020.21.4.654
Liver fluke infections is an important parasitic disease that common attack cattle, buffalo and others ruminant. The disease in the tropics is caused by Fasciola gigantica trematodes. Fasciolosis found in Bali cattle besides causing high economic loss also has the opportunity infect humans. This study aims to determine the pathology changes of Bali cattle hepatobiliary system infected by F. gigantica. In January2019 were examined a hundred of liver tissue Bali cattle. Based on the sex as many as 75 bali cattle are cows and 25 are bulls. On macroscopic examination was found fifteen bali cattles in gallbladder containing of F. gigantica. In positive Fasciolosis the surface of liver look uneven with enlargement of lymph nodes. The tissue of Bali cattle liver infected with F. gigantica is cut into 1x1x1 cm and put in a pot that has been filled with 10% neutral buffered formaline. The fixed tissue then processed in a tissue processor and stained with hematoxylin-eosin (HE). Microscopic examination was found bile ducts proliferation and blocked of the lumen accompanied by cholangio cirrhosis. Found longitudinal pieces of Fasciola giganticaliver fluke surrounded by fibrous connective tissue and multifocal coagulative necrosis with fibroblast proliferation. In some location the infiltration of neutrophil cells around the bile ducts can also be seen. Congestion, bleeding, and neutrophil infiltration are also seen in areas of necrosis. In the bile duct wall bleeding, adenomatus hyperplasia, infiltration of mononuclear cells with mild intensity and foci ofcalcification are found. It can be concluded that there was a change with severe intensity accompanied by chronic inflammation in the hepatobiliary system of Bali cattle with Fasciolosis.
Profil Leukosit Serta Imbangan Neutrofil dan Limfosit pada Kambing Peranakan Etawah yang Sedang Bunting
Sus Derthi Widhyari;
Setyo Widodo;
I Wayan Teguh Wibawan;
Anita Esfandiari;
Chusnul Choliq
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
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DOI: 10.19087/jveteriner.2020.21.4.581
This research aimed to determine the profiles of total leukocytes and neutrophil -lymphocyte ratio (N/L) in pregnant etawah crossbreed goats. Six etawah crossbreed goats around 3-6 years old and body weight around 30-50 kg were used in this study. Blood samples were taken from jugulars vein in non pregnant (K), and at gestational age 12, 14, 16 and 18 weeks. Blood is inserted into a tube that containsEDTA (Ethylene Diamine Tetraacetic Acid) anticoagulant to obtain whole blood. Whole blood were analyzed to total leukocyte cells and leukocyte types. The results showed that total leukocytes profiles tend to increase during pregnancy. Lymphocytes stable until 16 weeks of pregnancy and tends to decrease at the end of pregnancy. The N/L ratio increase at the end of pregnancy, this condition indicates an increase in the number of neutrophils accompanied by decreased lymphocyte cells and can be found in etawah crossbreedat 18 weeks of gestation.
Melacak Kekerabatan Brucella Sp. Menggunakan Teknik Reaksi Rantai Polimerase dengan Primer Pendek
Difa Widyasari;
Eko Sugeng Pribadi;
Fachriyan Hasmi Pasribu;
Widya Septiningtyas;
Jati Adiputra
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
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DOI: 10.19087/jveteriner.2020.21.4.493
Brucellosis is a zoonotic disease that has spread throughout the world and has an important impact on both human and animal health. The four species of Brucella that cause disease in humans are Brucella abortus, B. suis, B. melitensis and B. canis, and B. melitensis as the most pathogenic species. This Research used 46 samples were collected from Government Small Ruminants Abattoir in Bogor Regency. Thirty two spleen samples were examined by previous research and showed a positive result when were tested with CFT and PCR techniques, but sequencing has not yet been done. Fourteen serum and spleen samples were examined by the similar techniques. The Research aimed to determined the genetic relationship of Brucella sp. using a PCR technique with a specific short primer to B. mellitensis. Cloning technique was applied previously to five PCR positive samples before sequencing. Cloning and sequencing result of the Sample 91 showed higher homology to B. melitensis and B. abortus for 127 nucleotide lengths, 97.6% -100% and 99.2% -100% respectively. In the phylogenic tree, the Sample 91 was part of B melitensis sequences 1, 2,and 3 with accession numbers LT962930.1 and LT962936.1, B abortus sequences 1 and 2 with accession numbers CP033079.1 and B. abortus sequence 1 with accession number CP034695.1. Sample of 95, 97, 7, and 13 have lower homologies than Sample 91.
Ekstrak Minyak Kelapa Daun Gamal (Gliricidia sepium) Sebagai Akarisida Botani untuk Skabies pada Kambing
Dyah Haryuningtyas Sawitri;
Yuningsih Yuningsih
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
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DOI: 10.19087/jveteriner.2020.21.4.617
Scabies is a contagious parasitic skin disease caused by Sarcoptes scabiei. In Indonesia, this disease considerably remains a major problem in animal health, particularly in goats. The aim of this study is to investigate the effectivity of gamal leaves coconut oil extract (GLEC) for scabies treatment in goat. This research was divided into 2 parts: 1. Preparing Gamal Leaves Coconut Oil Extract (GLCE) which included4 stages of study: collection of gamal leaves; measurement coumarin level in gamal leaves; gamal leaves extraction with coconut oil; and extract stability test and 2. Application of GLCE in goats includes 3 stages of study : artificial S. scabiei infestations in goats; GLCE treatment in goats with 3 concentrations (12.5%, 25%, 50%); and drug safety testing. The results showed that the concentration of coumarin in the gamal leaves which was effectively for scabies drugs was > 1000 ppm. The 12.5%, 25%, 50% of GLCE concentrations are safe as botanical acariside and can reduce 100% of S.scabiei mite populations after 4x, 3x and 2x treatment at 1 week intervals, respectively. The 50% concentration of GLCE is safe, most effective, efficient and stable in storage for scabies drug.
Faktor-Faktor Risiko Kawin Berulang pada Sapi Potong di Kecamatan Licin, Kabupaten Banyuwangi, Jawa Timur
Putri Nurmalia Damayanti;
Trilas Sardjito;
Ragil Angga Prastiya
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
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DOI: 10.19087/jveteriner.2020.21.4.550
Nowadays, beef cattle farms in Licin Subdistrict, Banyuwangi Regency have problems in providing beef demand because the less of productivity. This problems occured due to the repeat breeding case and decrease the efficiency and productivity of livestock. It suspected because of the lack of hygine and sanitation from farmers and inseminator. This study aimed to correlate the factors and cases of repeat breeding on beef cattle in Licin Subdistrict, Banyuwangi Regency. It was a descriptive study which used 69 cow withsimple random sampling method. Primary data was obtained from direct observation of livestock, then continued with interviewing farmers using in-depth study system. While secondary data is obtained from the records of inseminator. It included the term of farming, farmer’s ability to detect estrus cycle, housingand the feeding of beef cattle. The collected data was analyzed by descriptive statistic, then processed by using SPSS 22.0. Chi Square Test (X2) was used to determine the relation between the causal factors and repeat breeding. The results of this study indicate the prevalence of repeat breeding in Licin Subdistrict, Banyuwangi Regency by 64%. All the factors such as the term of farming, farmer’s ability to detect estrus cycle, and feeding of beef cattle are correlated with the incidence of repeat breeding.
Gambaran Mikroskopis Berbagai Organ Merpati yang Menunjukkan Gejala Tortikolis dan Identifikasi Virus Penyakit Tetelo Berdasarkan Uji Agar Gel Presipitasi
Risang Aji Dewandaru;
Ferdinand Prayogo Cahyo Santoso;
Sugiyono Sugiyono;
Michael Haryadi Wibowo
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
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DOI: 10.19087/jveteriner.2020.21.4.519
This study aimed to determine the microscopic conditions of organs of pigeons that suffered from torticollis and identify the cause of disease in torticollis pigeons. Three pigeons showed torticollis symptoms were obtained as sample and marked as pigeon A, B and C, respectively. Isolation of pathogen with inoculation into embryonated pigeon eggs obtained from parents with no history of vaccination and not indicated ND. Identification of ND with agar gel precipitation (AGP) test. Observation of microscopical changes with histopathologic preparation using hematoxylin and eosin staining. Histopathological examination showed that pigeons was done severe neuritis vagus, trakheitis, pneumonia, air sacculitis, hepatitis, pankreatitis, nefritis, jejunoileitis, ileocolitis and orchitis. Perivascular cuffing found in brain. Degenerative changes found in the hepar and ren. Cardiac severe necrotic lesion, and depletion in white pulp area of spleen. Proventricular tissue showed flattening of mucosal epithelium, congestion lesions found in pulmonary tissue. The results of slow hemagglutination test of pigeon egg allantoic fluid, which tested for hemagglutination (HA) showed positive HA result with titers varying between 2, 32, and 64. Serological identification carried out with the AGP test on all culture samples against ND antiserum showing positive results of ND virus. Based on these dara, it can be concluded that the pigeons withsymptoms of torticollis is caused by ND virus.
Apoptosis Neuron Nukleus Merah pada Tikus Model Penyakit Parkinson Akibat Induksi Parakuat Diklorida
Ayu Miftahul Khasanah;
Niswah Nurul Fahma;
Tri Wahyu Pangestiningsih
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
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DOI: 10.19087/jveteriner.2020.21.4.588
Red nucleus plays a role in motoric control, so that disturbanced of neurons in this nucleus could be affected on motor impairment. Previous study reported that paraquat (PQ) could induced Parkinson disease, a neurodegeneratif disease that affected subtantia nigra pars compactca and clinically characterized by motoric disorders. Apoptosis is a programed cell death that physiologically normal for the body, but in large quantities will cause progresive cell death. This study aimed to observed apoptosis in rats RN neurons following by PQ injection. Ten male Wistar rats with the aged of 3 month, devided into two groups of five. Control group was given aquadest and treatment group was given PQ, injected intraperitoneally, dosage 7 mg/kg BW, twice a week for three weeks. At day 24th all rats were anesthetized using ketamin dosage 40 mg/kg BW and xylazine dosage 5 mg/kg BW, perfused intracardially using 4% paraformaldehyde and mesencephalons were collected. Mesencephalons were processed for histological preparations using the paraffin method and cut stereologically in coronal section, in 4 ?m thickness. Immunohistochemistry staining was done using caspase 3 antibody as a marker of apoptosis. Data were analyzed descriptively and quantitatively. The results in both groups showed RN neurons were large size and stelat or fusiform in shape. The percentage of apoptotic neurons in the RN of treatment group was significantly increased (80,4%±13,8%) compared to the control group (26,6%±18,32%) (P<0.01). In conclusion, following PQ exposurethere is a significant increased of apoptosis neurons number in the RN of rat model of Parkinson disease.