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All Journal Jurnal Analis Kesehatan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) JURNAL SKALA HUSADA: THE JOURNAL OF HEALTH
Dewi, Pande Putu Ayu Patria
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Identifikasi Cacing Pita (Taenia solium) dengan Metode Mikroskopis dan Nested PCR Rinawati, Luh Putu; Yanty, Jannah Sofi; Kurniawan, Surya Bayu; Bekti, Heri Setyo; Antarini, Anak Agung Nanak; Puryana, I Gusti Putu Sudita; Dewi, Pande Putu Ayu Patria; Rakhmawati, Aprilia
Jurnal Analis Kesehatan Vol. 13 No. 1 (2024): Jurnal Analis Kesehatan
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v13i1.4531

Abstract

Taenia solium merupakan parasit yang dapat menimbulkan infeksi taeniasis dan sistisekosis. Di Indonesia terutama di Bali kejadian taeniasis tinggi yang disebabkan kebiasan masyarakatnya mengonsumsi “lawar pork”. Daging babi yang terinfeksi dapat menjadi sumber penularan T. solium. Tujuan dari penelitian ini adalah untuk mengidentifikasi T. solium menggunakan metode mikroskopis dan Nested PCR. Penelitian merupakan penelitian deskriptif dengan desain observasional yang dilakukan terhadap 26 sampel yang diperiksa secara mikroskopis dan molekuler menggunakan Nested PCR dengan primer spesifik terhadap gen Tso31. Dari hasil penelitian menggunakan metode mikroskopis diperoleh sebanyak 21 sampel yang positif yang mengandung bagian cacing dan telur cacing, sedangkan dengan metode Nested PCR diperoleh 2 sampel positif yang memiliki berat molekul 234 bp. Identifikasi T. soliumpada manusia penting karena diperlukan untuk memberikan pengobatan tepat waktu pada penderita sekaligus untuk memutus rantai penularan parasit tersebut.
Inflammation Marker and Leukocyte Parameter Ratio on Erythropoiesis Activity of Pediatric Acute Lymphoblastic Leukemia Wande, I Nyoman; Linawati, Ni Made; Dewi, Pande Putu Ayu Patria; Ariawati, Ketut
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 31 No. 3 (2025)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v31i3.2395

Abstract

Acute leukemia is the most common malignancy found in children. A Complete Blood Count (CBC) examination can calculate the lymphocyte-to-leukocyte ratio, neutrophil-to-leukocyte ratio, and neutrophil-to-lymphocyte ratio to monitor the progress of Acute Lymphoblastic Leukemia (ALL). C-reactive protein is a marker of inflammation and can determine disease progression during treatment. The study's analytical design was observational, with a cross-sectional design conducted at the laboratory of the Clinical Pathology Faculty of Medicine, University of Udayana, and Ngoerah Hospital from March to August 2020. The study included 48 subjects, divided into three phases: induction, consolidation, and maintenance, in ALL patients. Specimen collection was collected in 2 tubes. Kolmogorov-Smirnov statistical analysis was used to assess normality, and homogeneity was evaluated with Levene’s test. The difference test was performed using the Kruskal-Wallis test. This study aimed to determine the relationship between parameters was performed with Spearman correlation. This study showed a strong correlation between hemoglobin levels and neutrophil/lymphocyte ratio (r= 0.358, p=0.015), hemoglobin and lymphocyte/leukocyte ratio (r= -0.287, p=0.048), hemoglobin and CRP (r= -0.493, p=0.000). Significant correlation between reticulocyte percentage and neutrophil/lymphocyte ratio (r= 0.505, p=0.000), neutrophil/lymphocyte ratio and CRP (r= -0.870, p=0.000), neutrophil/leukocyte ratio and lymphocyte/leukocyte ratio (r= 0.960, p=0.000). Significant correlations have been found between hemoglobin levels and the neutrophil/lymphocyte ratio (NLR), which is a measure of the lymphocyte/leukocyte ratio (LLeu-ratio), along with CRP. This relationship has been connected to inflammation in ALL patients following chemotherapy. CBC is an easy-to-use test to determine whether inflammation is present in ALL patients following chemotherapy.
ASPEK KLINIS DAN LABORATORIS PENYAKIT HEPATOMA Dewi, Pande Putu Ayu Patria; Anniwati, Leonita; Bekti, Heri Setiyo
Jurnal Skala Husada : The Journal of Health Vol 22, No 1 (2025): Jurnal Skala Husada (JSH): The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jsh:tjoh.v22i1.3811

Abstract

Hepatoma (karsinoma hepatoseluler) adalah kanker hati yang berasal dari hepatosit. Insiden hepatoma adalah 5,6 dari semua kanker yang menyerang manusia. Angka kematian dan kejadian hepatoma adalah 500.000 sampai 1 juta kasus setiap tahunnya. Faktor risiko hepatoma adalah virus hepatitis B (HBV), virus hepatitis C (HCV), sirosis hati, aflatoksin, obesitas, diabetes melitus, dan alkohol. Patogenesis hepatoma berasal dari faktor risiko dan berkembang menjadi fase kronis, infeksi yang disebabkan oleh HBV dan HCV akan menyebabkan inflamasi dan menghasilkan radikal bebas, sitokin, dan kemokin yang mengakibatkan kerusakan DNA sel, proliferasi sel, fibrosis, dan angiogenesis. Manifestasi klinis hepatoma adalah ikterus, ensefalopati hepatik, oedema anasarca, asites, dan varises. Diagnosis hepatoma ditegakkan dari anamnesis, pemeriksaan fisik, penanda tumor  seperti Alpha-1 Fetoprotein (AFP ) Lens Culinaris Agglutinin-Reactive AFP (AFP-L3) Des-g-carboxy prothrombin, Alpha-l-fucosidase (AFU), Glypican-3 (GPC3), Squamous cell carcinoma antigen (SCCA), Golgi protein 73, Hepatocyte growth factor (HGF), Transforming growth factor-beta 1 (TGF-b1), Vascular endothelial growth factor (VEGF), dan Proteomik serum serta biopsi.
Co-Authors Anniwati, Leonita Antarini, Anak Agung Nanak Ardhiana, Dewa Putu Yudhi Bekti, Heri Setyo Heri Setiyo Bekti I Nyoman Wande Ketut Ariawati Kurniawan, Surya Bayu Ni Made Linawati Rakhmawati, Aprilia Rinawati, Luh Putu Wiyata Putra, I Dewa Putu Gede Yanty, Jannah Sofi