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Quality Control Analysis of Creatinine Examination with Westgard Multi Rules and Six Sigma Wati, Wayan Shinta; Cahyani, Anak Agung Ayu Eka; Prihatiningsih, Diah; Parwati, Putu Ayu
Basic and Applied Nursing Research Journal Vol. 6 No. 1 (2025): Basic and Applied Nursing Research Journal (BANRJ)
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/banrj.06.01.05

Introduction: Creatinine is an examination parameter for kidney function and can be measured by several methods. Quality control needs to be done to ensure the results of the creatinine examination are correct. Objective: This study aims to determine the results of the quality control analysis of creatinine examination with Westgard multirules and Six Sigma in the clinical pathology laboratory at Wangaya Hospital, Denpasar, in 2023. Methods: This study used a descriptive research design, secondary data, and quality control results. Data analysis was done by determining the mean value, SD, CV, d%, sigma, and making the Levey-Jennings graph. The results showed that the bias value (d%) was still below the maximum value; the CV value in January, March, April, and July exceeded the maximum limit. Evaluation of Westgard's rules obtained the results of violations of trends, shifts, and 1- 2s, then variations in sigma values each month, namely <3 and >4 from June and September to December 2023. Results: Based on the results of the study, it can be concluded that creatinine examination obtained a good accuracy value, low precision value in certain months, there are random (1- 2s) and systematic (trend and shift) errors, and a low sigma value, which is <3 (unacceptable performance and poor performance categories). However, the sigma value has increased from June to September to December, which is >3 (marginal performance, good performance, and world-class performance categories). Conclusion: Accuracy and precision values must be maintained because good accuracy and precision values will increase the sigma value, especially in creatinine testing.

PERBEDAAN SKOR BASIL TAHAN ASAM PADA PEMERIKSAAN SPUTUM TEKNIK ZIEHL-NEELSEN KONVENSIONAL DAN PENAMBAHAN BLEACH 0,5% Ayudin, Ayudin; Bintari, Ni Wayan Desi; Parwati, Putu Ayu
Jurnal Analis Laboratorium Medik Vol 10 No 2 (2025): Jurnal Analis Laboratorium Medik
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v10i2.6337

Latar belakang: Kualitas spesimen sputum berperan krusial dalam pemeriksaan mikroskopis basil tahan asam (BTA). Pada pasien tuberkulosis paru, dahak yang melewati tenggorokan sering mengandung debris mikroskopis yang dapat mengganggu kualitas pewarnaan. Penggunaan bleach sebagai dekontaminan dinilai mampu meminimalkan gangguan tersebut dengan membersihkan lapang pandang mikroskopis. Tujuan: membandingkan skor BTA pada pewarnaan Ziehl Neelsen konvensional dan dengan penambahan bleach 0,5%. Metode: eksperimental yang dilakukan di UPTD Puskesmas Gonenggati Donggala pada Januari -April 2025. Sampel pada penelitian ini adalah sputum dari pasien dengan suspek TB paru di UPTD Puskesmas Gonenggati Donggala sebanyak 67 sampel. Pemeriksaan BTA dilakukan dengan metode pengecatan Ziehl Nelseen. Hasil: hasil pemeriksaan BTA dengan metode Ziehl Nelseen konvensional didapatkan skor negatif (83,6%), scanty (0 %), positif 1 (10,4%), positif 2 (3,0%) dan positif 3 (3,0%). Sementara itu hasil pemeriksaan BTA dengan metode Ziehl Nelseen dengan penambahan bleach 0,5% didapatkan skor negatif (82,6%), scanty (1,5%), positif 1 (10,4%), positif 2 (3,0%) dan positif 3 (3,0%). Hasil uji beda dengan Wilcoxon Range Test didapatkan hasil nilai p > 0,665. Kesimpulkan: bahwa tidak ada perbedaan hasil skor basil tahan asam pada pewarnaan Ziehl Nelseen konvensional dengan Ziehl Nelseen dengan penambahan bleach 0,5%.

Perbedaan Kadar Bilirubin Total Dengan Paparan Cahaya Yang Segera Diperiksa dan Ditunda Selama 3 jam Dewi, Made Gita Candra; Devhy, Ni Luh Putu; Parwati, Putu Ayu
Journal of Medical Laboratory Research Vol. 4 No. 1 (2025): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

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Total bilirubin examination serves to determine liver and bile duct function. Pathological hyperbilirubinemia can cause central nervous system disorders to death. Due to the importance of bilirubin results for supporting diagnosis, examinations must follow procedures. However, in the field, there are often issues like lack of control, damaged equipment, and others, resulting in delayed specimens. This study aims to determine the difference in total bilirubin levels with light exposure examined immediately and delayed for 3 hours. This experimental research used 36 sample units from 18 samples with 2 treatment groups: serum exposed to light examined immediately and serum exposed to light delayed for 3 hours. Sampling technique: accidental sampling. Data analysis used the Wilcoxon test. The results showed an average total bilirubin level of 3.1444 mg/dL for immediate examination and 3.1044 mg/dL for delayed examination. The data analysis result p-value 0.000, indicates a significant difference in total bilirubin levels between immediate and delayed examination. This difference occurs due to external and internal factors. External factors include light exposure, storage time, and inappropriate storage temperature. The blue light in lamps can affect the results, leading to a decrease in total bilirubin levels.

Perbedaan Kadar Kolestrol Total Pada Pengonsumsi Kopi dan Bukan Pengonsumsi Kopi Di Banjar Juwet Abiansemal Astuti, Ni Kadek ary; Parwati, Putu Ayu; Cahyani, Anak Agung Ayu Eka
Journal of Medical Laboratory Research Vol. 4 No. 1 (2025): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

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Coffee contains bioactive compounds such as caffeine, cafestol, and kahweol that can influene lipid metabolism. Consumption of unfiltered coffee (boiled coffee) has the potential to increase cholesterol levels. This study aimed to determine the difference in total cholesterol levels between coffee consumers in Banjar Juwr, Abiansemal. The research employed a quantitative comparative method with an observational design. A total of 70 male respondents age 20-50 years (35 coffee consumers and 35 non-consumers) were selected using purposive sampling. Total cholesterol levels were measured using the test strip method (Easy Touch GCU). Data were analyzed using the Independent Sample T-Test. The mean total cholesterol levels of coffee consumers was 170.49 mg/dL, while that of non-consumers was 178.80 mg/dL. Statistical analysis showed p=0.103 (p>0.05), indicating no significant difference between the two groups. Conclusion Black coffee consumption in Banjar Juwet was not proven to significantly affect total cholesterol levels. Other factors such as dietary patterns, phhysical activity, and health history are presumed to play a greater role.

Effect of Variation in Examination Delay Time on Hematocrit Results Dian Kirana Dewi, Gusti Ayu Wulan; Parwati, Putu Ayu; Thrisna Dewi, Ni Luh Putu
Jurnal Kesehatan Cendikia Jenius Vol. 3 No. 2 (2026): April 2026
Publisher : CV. CENDIKIA JENIUS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70920/jenius.v3i2.397

Hematocrit examination is an important parameter in assessing the balance of red blood cells; however, delays in examination frequently occur in laboratories and may affect result accuracy. Therefore, this study is necessary to determine the impact of delayed examination time on hematocrit results. The purpose of this study was to determine the effect of variations in examination delay time on hematocrit results. This study used an experimental design with a sample size of 10 respondents selected using purposive sampling. Data collection was conducted through immediate hematocrit examination and after a 2-hour delay in December 2025, then analyzed using the Shapiro-Wilk test and Paired Sample T-Test. The results showed that the mean hematocrit value for immediate examination was 42.0% and decreased to 41.8% after the delay, with a difference of 0.2%. Statistical analysis showed a p-value of 0.000 (p<0.05), indicating a significant effect of delay time on hematocrit results. However, the changes were relatively small and not clinically significant. In conclusion, examination delay affects blood sample stability; therefore, it should be considered in laboratory quality control, and further research using different methods is recommended.

PERBEDAAN SKOR BASIL TAHAN ASAM PADA PEMERIKSAAN SPUTUM TEKNIK ZIEHL-NEELSEN KONVENSIONAL DAN PENAMBAHAN BLEACH 0,5% Ayudin, Ayudin; Bintari, Ni Wayan Desi; Parwati, Putu Ayu
Jurnal Analis Laboratorium Medik Vol 10 No 2 (2025): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

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Latar belakang: Kualitas spesimen sputum berperan krusial dalam pemeriksaan mikroskopis basil tahan asam (BTA). Pada pasien tuberkulosis paru, dahak yang melewati tenggorokan sering mengandung debris mikroskopis yang dapat mengganggu kualitas pewarnaan. Penggunaan bleach sebagai dekontaminan dinilai mampu meminimalkan gangguan tersebut dengan membersihkan lapang pandang mikroskopis. Tujuan: membandingkan skor BTA pada pewarnaan Ziehl Neelsen konvensional dan dengan penambahan bleach 0,5%. Metode: eksperimental yang dilakukan di UPTD Puskesmas Gonenggati Donggala pada Januari -April 2025. Sampel pada penelitian ini adalah sputum dari pasien dengan suspek TB paru di UPTD Puskesmas Gonenggati Donggala sebanyak 67 sampel. Pemeriksaan BTA dilakukan dengan metode pengecatan Ziehl Nelseen. Hasil: hasil pemeriksaan BTA dengan metode Ziehl Nelseen konvensional didapatkan skor negatif (83,6%), scanty (0 %), positif 1 (10,4%), positif 2 (3,0%) dan positif 3 (3,0%). Sementara itu hasil pemeriksaan BTA dengan metode Ziehl Nelseen dengan penambahan bleach 0,5% didapatkan skor negatif (82,6%), scanty (1,5%), positif 1 (10,4%), positif 2 (3,0%) dan positif 3 (3,0%). Hasil uji beda dengan Wilcoxon Range Test didapatkan hasil nilai p > 0,665. Kesimpulkan: bahwa tidak ada perbedaan hasil skor basil tahan asam pada pewarnaan Ziehl Nelseen konvensional dengan Ziehl Nelseen dengan penambahan bleach 0,5%.

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