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LIVABILITY AND RECOVERY RATE OF BALI CATTLE SPERMATOZOA DURING PRESERVATION IN TRIS-BASED EGG YOLK DILUENT WITH DIFFERENT SUCROSE LEVELS Yendraliza, Yendraliza; Tania, Tania; Misrianti, Restu; Zumarni, Zumarni
Jurnal Kedokteran Hewan Vol 13, No 2 (2019): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i2.13033

Abstract

This study aimed to determine the effect of sucrose addition in tris-based egg yolk diluent in maintaining and protecting spermatozoa during preservation. The design of this study was a completely randomized design (CRD) which consisted of five treatments with ten replications. The treatments were tris-based egg yolk diluent without sucrose (P0, control), tris-based egg yolk diluent with 0.2% sucrose (P1), tris-based egg yolk diluent with 0.3% sucrose (P2), tris-based egg yolk diluent with 0.4% sucrose (P3) and tris-based egg yolk diluent with 0.5% sucrose (P4). The parameters measured were motility, livability, abnormality, intact plasma membrane, and recovery rate. Semen was evaluated 2 times, freshly after being collected and post thawed. The results showed that the increase level of sucrose addition from 0.2% to 0.5% increased the motility value from 43.59% to 48.15%, the livability value from 51.24% to 55.45%, the intact plasma membrane value from 44.66% to 48.21%, the recovery rate value from 54.6% to 60.2 %, and reduce the abnormality value from 13.49%-10.24%. It can be concluded that the addition of 0.2- 0.5% sucrose in tris-based egg yolk diluent could increase motility, livability, intact plasma membrane, recovery rate, and could reduce the abnormalities of Bali cattle spermatozoa during preservation
Co-Authors Adib C, Nino Ammar, Farikh Marzuki Antie, Santri Arming G, Andi Lis Clarissa, Clarissa Daddi, Herlina Debi, Puspita Dewi Ezha, Vira Gustina, Novia Hariandi, Ahmad Herman Ruslim Kartika Ningsih, Silvia Kosdamika, Yuhandra Conli Lestaria, Farmingga Lia Dwi Jayanti Mariana Mariana Markhmadov, Zhansaya K. Muhammad Yusuf Nehemia, Andika Paramita, Ursula Natasya Pradnya Pitriyana, Sisi Prasetiawati, Prasetiawati Raymond Raymond Restu Misrianti Riana, Marda Risnina Wafiqoh Rosifa.S, Hamidah Salsabila, Salma SEPTIANA SEPTIANA Syatriana, Eny Wijaya, Ardi Anjungie Wahyu Yendraliza - Zumarni, Zumarni