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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary
Sarudji, Suryanie
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : journal of Basic Medical Veterinary

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PENETAPAN RETENTION TIME PROGESTERON DALAM PELARUT ELUEN PHASE MOBILE MENGGUNAKAN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Chandra, Evania Haris; Lazuardi, Mochamad; Sarudji, Suryanie; Maslachah, Lilik; Sukmanadi, Mohammad; Utama, Suzanita
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21090

Abstract

The aim of this research was to determine the level of stability of Progesterone-retention time on Eluen Phase Mobile (EPM) solution in storage period for six, eight and 12 hours using High Performance Liquid Chromatography (HPLC). The research method used posttest-only control group design by using three treatments and six repetitions. The three repetitions consist into six hours, eight hours and 12 hours. The data were obtained analyzed by Summery Independent T-Test with SPSS 24 for windows. The results showed that the progesterone treatment in EPM solvents with six-hour storage (P1) and eight hours (P2) retention time progesterone are stable but the progesterone treatment in EPM solvent with 12-hour storage (P3) showed an unstable due to storage time. Besides that there are irrelevant peak which show that many pure molecular fractions are separated in the stationary phase so that the molecular structure of the substance begins to break down. Based on these results, it can be concluded that the storage time of Progesterone in Eluen Phase Mobile began to be unstable at 12 hours of storage. 
Most Probable Number of Coliform on Beef from Krian Abattoir, Sidoarjo Pratiwi, Sambang Dewi; Harijani, Nenny; Sarudji, Suryanie; Budiarto, Budiarto; Estoepangestie, Soelih
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.36476

Abstract

The problem on food health is the high amount of bacterial contamination of food served by food industries. The quality of beef can be affected by the level of bacterial contamination in meat. Coliform is a group of bacteria used as a sanitary hygiene indicator. The purpose of this study is to measure the value of MPN Coliform in beef at Krian's abattoir of district Sidoarjo. Thirty samples were taken from the abattoir of Krian, Sidoarjo. Most Probable Number was used to determine the level of Coliform's contaminate beef. The results showed that all beef samples at abattoir were contaminated with Coliform. The average contaminant levels was 706,76 MPN/g. Twenty six samples exceed the maximum limit of Coliform bacteria contamination in accordance with Indonesian National Standard (SNI). The highest Coliform MPN value is ≥ 1600 MPN / g, whereas the lowest MPN Coliform value is 8 MPN/g.
Co-Authors Adiana Mutamsari Witaningrum Benjamin Christoffel Tehupuring, Benjamin Christoffel Budiarto Budiarto Chandra, Evania Haris Dwi, Welyanto Kurniawan Estoepangestie, Agnes Theresia Soelih Estoepangestie, Soelih Habibi, Muhammad Afif Hamid, Iwan Sharial Hani Plumeriastuti Harijani, Nenny Iwan Sahrial Hamid Kurniawati, Sepvian Dewi Lilik Maslachah Mochamad Lazuardi Murjianingsih, Fitriani Mustofa Helmi Effendi Praja, Ratih Novita Prameswari, Ratna Ajeng Pratiwi, Sambang Dewi Saputro, Amung Logam Sukmanadi, Mohammad Suzanita Utama Tyasningsih, Wiwiek Widjiati Widjiati, Widjiati Yudhana, Aditya Yunita, Maya Nurwartanti